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The map label for this gene is aroE

Identifier: 209693707

GI number: 209693707

Start: 84984

End: 85805

Strand: Direct

Name: aroE

Synonym: VSAL_I0083

Alternate gene names: 209693707

Gene position: 84984-85805 (Clockwise)

Preceding gene: 209693706

Following gene: 209693708

Centisome position: 2.56

GC content: 38.81

Gene sequence:

>822_bases
ATGGATAAGTATGCAGTCTTTGGTCATCCCATTAAGCAAAGCAAATCTCCTTTTATTCATACATTATTCGCTCGTCAAAC
AATACAACCTTTGGAATATGTTGCTATTGAGGCTCCAGTGGATGGCTTTCTTAATTCGGTAACTCATTTTTTTTCTAATG
GGGGTAAAGGCTGTAATATTACCGTACCCTTTAAAGAAGAAGCATTTCAATTTGCCGATCAATTAACAGATAGAGCAAAA
TTGGCTGGTGCGGTTAATACGCTAAAAAAGCTGGATGATGGAATTATTCTCGGTGATAACACGGACGGTGAAGGATTAGT
TCAAGACTTACTGCAATATCAGGTACCACTTCAAGATAAGCGTATCTTATTAATTGGAGCAGGAGGGGCAGCACGAGGTG
TACTTCTTCCCTTATTAAAACAAAACCCCTCTTCTATTACCGTAGTTAATCGCACTTATGAAAAAGCAGAAGCACTAGCT
GAAATATTTTCAGCGTATGGTGCTGTAGAGGCGGTTCGCATGAGTGACGTGACACGATCGTTTGATGTGATAATTAATTC
AACGTCTGCCAGTTTATCTGGAGAGTTACCTAATATTAGTCCAGTGATTTTTTCTAAAGGCAGTATCAGCTATGACATGA
TGTATGGCAAAGGTAAAACGGTCTTTAATCAATGGGCATTAGATAATGATGTATACCAAGCTTATGATGGATTAGGAATG
TTAGTTGGGCAAGCTGCGGAAAGCTTTACTGTATGGAGAGGATTACGTCCTAGCAGCAAACAAATACTACGAGAACTTCG
CAAAAATTTAGAAGGTATGTAA

Upstream 100 bases:

>100_bases
TGCAAAAACTGGAGAGACATTACGCCAAGGCTAATGCATCTACTATTTCTTTCAGGTAAGGTTAGTTCAGTTTAACTTTA
ATCATTGAAAGGAATCACTC

Downstream 100 bases:

>100_bases
TGAATCAATTCTCTTTTCTGATGGGCAACAATGGAACCCTGAAAAGCAATGTATTATTTTATATGCTCAACAAATGGGTA
ATTTAATACCATGTCTTGTC

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MDKYAVFGHPIKQSKSPFIHTLFARQTIQPLEYVAIEAPVDGFLNSVTHFFSNGGKGCNITVPFKEEAFQFADQLTDRAK
LAGAVNTLKKLDDGIILGDNTDGEGLVQDLLQYQVPLQDKRILLIGAGGAARGVLLPLLKQNPSSITVVNRTYEKAEALA
EIFSAYGAVEAVRMSDVTRSFDVIINSTSASLSGELPNISPVIFSKGSISYDMMYGKGKTVFNQWALDNDVYQAYDGLGM
LVGQAAESFTVWRGLRPSSKQILRELRKNLEGM

Sequences:

>Translated_273_residues
MDKYAVFGHPIKQSKSPFIHTLFARQTIQPLEYVAIEAPVDGFLNSVTHFFSNGGKGCNITVPFKEEAFQFADQLTDRAK
LAGAVNTLKKLDDGIILGDNTDGEGLVQDLLQYQVPLQDKRILLIGAGGAARGVLLPLLKQNPSSITVVNRTYEKAEALA
EIFSAYGAVEAVRMSDVTRSFDVIINSTSASLSGELPNISPVIFSKGSISYDMMYGKGKTVFNQWALDNDVYQAYDGLGM
LVGQAAESFTVWRGLRPSSKQILRELRKNLEGM
>Mature_273_residues
MDKYAVFGHPIKQSKSPFIHTLFARQTIQPLEYVAIEAPVDGFLNSVTHFFSNGGKGCNITVPFKEEAFQFADQLTDRAK
LAGAVNTLKKLDDGIILGDNTDGEGLVQDLLQYQVPLQDKRILLIGAGGAARGVLLPLLKQNPSSITVVNRTYEKAEALA
EIFSAYGAVEAVRMSDVTRSFDVIINSTSASLSGELPNISPVIFSKGSISYDMMYGKGKTVFNQWALDNDVYQAYDGLGM
LVGQAAESFTVWRGLRPSSKQILRELRKNLEGM

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=271, Percent_Identity=50.1845018450184, Blast_Score=269, Evalue=1e-73,
Organism=Escherichia coli, GI1787983, Length=270, Percent_Identity=28.1481481481481, Blast_Score=95, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6320332, Length=284, Percent_Identity=27.8169014084507, Blast_Score=81, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_ALISL (B6EP29)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261635.1
- ProteinModelPortal:   B6EP29
- GeneID:   6986300
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0083
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- ProtClustDB:   PRK00258
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 29876; Mature: 29876

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKYAVFGHPIKQSKSPFIHTLFARQTIQPLEYVAIEAPVDGFLNSVTHFFSNGGKGCNI
CCCCEECCCCHHCCCCCHHHHHHHHHHCCCHHEEEEECCHHHHHHHHHHHHHCCCCCCEE
TVPFKEEAFQFADQLTDRAKLAGAVNTLKKLDDGIILGDNTDGEGLVQDLLQYQVPLQDK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCCC
RILLIGAGGAARGVLLPLLKQNPSSITVVNRTYEKAEALAEIFSAYGAVEAVRMSDVTRS
EEEEEECCCCCCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDVIINSTSASLSGELPNISPVIFSKGSISYDMMYGKGKTVFNQWALDNDVYQAYDGLGM
EEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHH
LVGQAAESFTVWRGLRPSSKQILRELRKNLEGM
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKYAVFGHPIKQSKSPFIHTLFARQTIQPLEYVAIEAPVDGFLNSVTHFFSNGGKGCNI
CCCCEECCCCHHCCCCCHHHHHHHHHHCCCHHEEEEECCHHHHHHHHHHHHHCCCCCCEE
TVPFKEEAFQFADQLTDRAKLAGAVNTLKKLDDGIILGDNTDGEGLVQDLLQYQVPLQDK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHCCCCCCC
RILLIGAGGAARGVLLPLLKQNPSSITVVNRTYEKAEALAEIFSAYGAVEAVRMSDVTRS
EEEEEECCCCCCHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FDVIINSTSASLSGELPNISPVIFSKGSISYDMMYGKGKTVFNQWALDNDVYQAYDGLGM
EEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHH
LVGQAAESFTVWRGLRPSSKQILRELRKNLEGM
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA