Click here to switch to the map view.

The map label for this gene is traC [H]

Identifier: 209693654

GI number: 209693654

Start: 22530

End: 23489

Strand: Direct

Name: traC [H]

Synonym: VSAL_I0021

Alternate gene names: 209693654

Gene position: 22530-23489 (Clockwise)

Preceding gene: 209693653

Following gene: 209693656

Centisome position: 0.68

GC content: 39.9

Gene sequence:

>960_bases
ATGACTTATTCAACCACTATCAATTCAACAATCACTAAAGAAACAGAAGCGACTAAAGCAAGAGCAGGCTATCCCAAAAA
TGAAATGGTTAAAAAGCGTAAAACAACAAAAAAACGCGCAGTCGTAAAAGTATCTAAAGACATTTATCAAATGGTGACTG
ATCGCATTATTGCAGCATTAGAAAGTGGAGTTAAACCGTGGGCGTGTCCGTGGGATAGAACGAATGAATGCTCAATGTTA
CCGATGAACTTTAAAACAAAAGCGAATTATTCAGGGGTAAATATTCTACTGCTTTGGAGTGAAACGGTGATGAAAGGTTT
CACAAGCCCTTACTGGTTGACCTATAACCAAGCCGTTGAGCTAGGCGGTAACGTTATCAAAGGCCAAAAAGGAACCCCAA
TTATCTATTATAAAAATTGGGAAAAAGAAAACGACGACGGAAAAACAGAAATTATCCCAATGTTAAAAACCTTTACTGTG
TTTAATCTTGATCAAGTAGAAGGCATCGAAAAGCCAGCGGTTACAGTGGCAGAAGTGAGAGAGAAAAACGAATTTGTAAC
GTTAGATCACGTAGTCGAGGCCATCAATGCAACTGGAATTAAAATCAATCATTGTGGTATTCGTGCGTTTTATTCGTCTG
CACACGATGAAATCACATTGCCTATGGTTGACCGTTTTAATTCTTCTTATGACTATTACGCGACGACATGGCATGAATTG
GTTCACGCAACAGGTCACAAAACCCGTTTAGACCGTAATTTAAAAAACAGCTTTGGTTCTAAAGATTATGCGTTTGAGGA
ATTGATTGCCGAGTTAGGCGCAGAGTTCTGCTGTGCTGATTTGGGGATTGTGGGCGAGGTGCAACATGAAAGCTATATCG
CAAGCTGGCTAGAGCGTTTGAATGGCGATAAGAAATTTATTTTTAAAGCCGCCAGTCAAGCAAGGCGCATCAATGGCTAA

Upstream 100 bases:

>100_bases
TTCGCCGCGTTGCGCTTTTGCAAGGCAGAACCATGTTAGGAGTTCGGACAGTCAGGCGAAGACGAAGATGAAAACTCACT
TAAACATAGGAAAAACAGCC

Downstream 100 bases:

>100_bases
TTAAACGATAAAAAAGAAAAGGCTTAAAGCACTTATATGACAAGTAACTTTAAGCCTAAACCACTTAACTAACGTAAGGA
ATATAAAATTATGACCGATA

Product: antirestriction protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTYSTTINSTITKETEATKARAGYPKNEMVKKRKTTKKRAVVKVSKDIYQMVTDRIIAALESGVKPWACPWDRTNECSML
PMNFKTKANYSGVNILLLWSETVMKGFTSPYWLTYNQAVELGGNVIKGQKGTPIIYYKNWEKENDDGKTEIIPMLKTFTV
FNLDQVEGIEKPAVTVAEVREKNEFVTLDHVVEAINATGIKINHCGIRAFYSSAHDEITLPMVDRFNSSYDYYATTWHEL
VHATGHKTRLDRNLKNSFGSKDYAFEELIAELGAEFCCADLGIVGEVQHESYIASWLERLNGDKKFIFKAASQARRING

Sequences:

>Translated_319_residues
MTYSTTINSTITKETEATKARAGYPKNEMVKKRKTTKKRAVVKVSKDIYQMVTDRIIAALESGVKPWACPWDRTNECSML
PMNFKTKANYSGVNILLLWSETVMKGFTSPYWLTYNQAVELGGNVIKGQKGTPIIYYKNWEKENDDGKTEIIPMLKTFTV
FNLDQVEGIEKPAVTVAEVREKNEFVTLDHVVEAINATGIKINHCGIRAFYSSAHDEITLPMVDRFNSSYDYYATTWHEL
VHATGHKTRLDRNLKNSFGSKDYAFEELIAELGAEFCCADLGIVGEVQHESYIASWLERLNGDKKFIFKAASQARRING
>Mature_318_residues
TYSTTINSTITKETEATKARAGYPKNEMVKKRKTTKKRAVVKVSKDIYQMVTDRIIAALESGVKPWACPWDRTNECSMLP
MNFKTKANYSGVNILLLWSETVMKGFTSPYWLTYNQAVELGGNVIKGQKGTPIIYYKNWEKENDDGKTEIIPMLKTFTVF
NLDQVEGIEKPAVTVAEVREKNEFVTLDHVVEAINATGIKINHCGIRAFYSSAHDEITLPMVDRFNSSYDYYATTWHELV
HATGHKTRLDRNLKNSFGSKDYAFEELIAELGAEFCCADLGIVGEVQHESYIASWLERLNGDKKFIFKAASQARRING

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 36187; Mature: 36055

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSTTINSTITKETEATKARAGYPKNEMVKKRKTTKKRAVVKVSKDIYQMVTDRIIAAL
CCEECCCCCHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESGVKPWACPWDRTNECSMLPMNFKTKANYSGVNILLLWSETVMKGFTSPYWLTYNQAVE
HHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECHHHHHHCCCCEEEEEHHHHH
LGGNVIKGQKGTPIIYYKNWEKENDDGKTEIIPMLKTFTVFNLDQVEGIEKPAVTVAEVR
CCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEECCHHHCCCCCCCHHHHHHH
EKNEFVTLDHVVEAINATGIKINHCGIRAFYSSAHDEITLPMVDRFNSSYDYYATTWHEL
HCCCEEEHHHHHHHHHCCCEEEECCHHHHHHHCCCCCEEEHHHHCCCCCCCHHHHHHHHH
VHATGHKTRLDRNLKNSFGSKDYAFEELIAELGAEFCCADLGIVGEVQHESYIASWLERL
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCEECHHHHHHHHHHHHHH
NGDKKFIFKAASQARRING
CCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TYSTTINSTITKETEATKARAGYPKNEMVKKRKTTKKRAVVKVSKDIYQMVTDRIIAAL
CEECCCCCHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESGVKPWACPWDRTNECSMLPMNFKTKANYSGVNILLLWSETVMKGFTSPYWLTYNQAVE
HHCCCCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEEEECHHHHHHCCCCEEEEEHHHHH
LGGNVIKGQKGTPIIYYKNWEKENDDGKTEIIPMLKTFTVFNLDQVEGIEKPAVTVAEVR
CCCCEEECCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHEECCHHHCCCCCCCHHHHHHH
EKNEFVTLDHVVEAINATGIKINHCGIRAFYSSAHDEITLPMVDRFNSSYDYYATTWHEL
HCCCEEEHHHHHHHHHCCCEEEECCHHHHHHHCCCCCEEEHHHHCCCCCCCHHHHHHHHH
VHATGHKTRLDRNLKNSFGSKDYAFEELIAELGAEFCCADLGIVGEVQHESYIASWLERL
HHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHCCCCEECHHHHHHHHHHHHHH
NGDKKFIFKAASQARRING
CCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]