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The map label for this gene is gltX [H]

Identifier: 209554403

GI number: 209554403

Start: 869539

End: 870987

Strand: Reverse

Name: gltX [H]

Synonym: UUR10_0704

Alternate gene names: 209554403

Gene position: 870987-869539 (Counterclockwise)

Preceding gene: 209554027

Following gene: 209554507

Centisome position: 99.6

GC content: 26.64

Gene sequence:

>1449_bases
ATGAAAATTAGAACACGTTATGCTCCATCACCAACTGGTTATTTACATATTGGTGGAGCGCGAACCGCTTTGTTTAATTA
TTTATTAGCTAAAGCTTATGATGGTGATTTCATTATTCGCATTGAAGATACTGATGTTGAAAGAAATGTGGAAGGTGGAA
TTGATTCTCAATTTAATTTTTTAGAATGAATGGGAATTGTTGCTGATGAATCAATTCGTAATCCTAAAACATTTGGACCA
TATATTCAATCACAAAAACTAAAGCATTATGAAGCATTAGCTTTAGATTTAGTTGCACAAAAAAAAGCTTATTTTTGCTT
TTGTAGTAAAGAACGATTAGATGCTGATCGTGAATTAGCTGAAAAATTACATGAAACACCTAAATATAAACGTCATTGTT
TAAATTTAAATGAACAAACAATTCAAGCCAATTTATTAGCAAATAAAGAATATACAATTCGTTTAAAAATTGATGAAAAC
AACGAGTATAGTTGAGATGATTTAATTCGGGGTAAAATCAGTATTCCAGGCAGTGCTTTAACCGATCCTGTTATTTTAAA
GTCAAATAAAATTGCAATGTATAATTTCGCTGTTGTAATCGATGATTATGAAATGCAAATTTCGCATGTTATTCGTGGCG
AAGAACATATTTCTAATACGCCTTATCAATTAGCAATTGCGCAAGCGCTAAATTATGATTTATCTAAAATTAAATATGGT
CATTTATCAATTATTGTTGATGAAACAGGTAAAAAATTATCAAAGCGTAATTTAGCTTTAAAACAATTCGTATCTGATTA
TGAAAAAGATGGCTATTGACCACATGCTATTACTAATTTTGTAGCATTATTAGGTTGAAGTCCAAAAAATAATCAAGAGA
TTATGAGTTTAGTAGAAATGGTTGAGAATTTTGATGTTAATAATTTATCAAAATCACCTGCTTTTTTTGATATTAATAAA
ATGAATTGATTTTCAACACAATACTTTAATAATATTTCACAAGATGAATTTATTGATTTTGTTAAGACTCATCCACTAAC
AAAAGAATTAGTTTTAAAAGATACTACTTTCATTGATAAAGCACTATTATTTAAATCACACATTGTTAATTTAAAACAAT
TAATTAATTTAGTTGATGAACAATTTAATTCTAATAAACAATTATTAGAAGAAGATGTAAATCATATTAAAAATAATCAA
CTAACAAATGTTGTACAAGTTTTTTATGAACAATTAATAGTTAGTGAAAAATTTGATGAACAATCAATCAAAGAAGTAAT
TAAGCAAGTTCAAAAAACAACAAACAATAAAGGTGCTAATTTATACATGCCAATTCGAATTGCTACAACGTTTAGTTCGC
ATGGTCCTGAATTAGCGAAAACTATTTATTATTTAGGACGTGAAAATGTATTAAAAAATTTACAAAGTATTTTAAAAGTT
TTAGGTTAG

Upstream 100 bases:

>100_bases
TATAACGAATTATTGAGAGAGGGTTTTTATACAATTTTAAGCGGAGCAGGTAGCTCATTTATTTGCATAAAATTAAAAGA
TAAGGAAAATCAAGTTATTC

Downstream 100 bases:

>100_bases
TTGCGTTATGGTAAAAAATTTTTTTATAAAAGATCCTATTCATGGTTTAATCTCTTTTGATGAGCAATATGAATGAGCTT
ATGAGCTAATTAAGACAAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYDGDFIIRIEDTDVERNVEGGIDSQFNFLEWMGIVADESIRNPKTFGP
YIQSQKLKHYEALALDLVAQKKAYFCFCSKERLDADRELAEKLHETPKYKRHCLNLNEQTIQANLLANKEYTIRLKIDEN
NEYSWDDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDLSKIKYG
HLSIIVDETGKKLSKRNLALKQFVSDYEKDGYWPHAITNFVALLGWSPKNNQEIMSLVEMVENFDVNNLSKSPAFFDINK
MNWFSTQYFNNISQDEFIDFVKTHPLTKELVLKDTTFIDKALLFKSHIVNLKQLINLVDEQFNSNKQLLEEDVNHIKNNQ
LTNVVQVFYEQLIVSEKFDEQSIKEVIKQVQKTTNNKGANLYMPIRIATTFSSHGPELAKTIYYLGRENVLKNLQSILKV
LG

Sequences:

>Translated_482_residues
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYDGDFIIRIEDTDVERNVEGGIDSQFNFLE*MGIVADESIRNPKTFGP
YIQSQKLKHYEALALDLVAQKKAYFCFCSKERLDADRELAEKLHETPKYKRHCLNLNEQTIQANLLANKEYTIRLKIDEN
NEYS*DDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDLSKIKYG
HLSIIVDETGKKLSKRNLALKQFVSDYEKDGY*PHAITNFVALLG*SPKNNQEIMSLVEMVENFDVNNLSKSPAFFDINK
MN*FSTQYFNNISQDEFIDFVKTHPLTKELVLKDTTFIDKALLFKSHIVNLKQLINLVDEQFNSNKQLLEEDVNHIKNNQ
LTNVVQVFYEQLIVSEKFDEQSIKEVIKQVQKTTNNKGANLYMPIRIATTFSSHGPELAKTIYYLGRENVLKNLQSILKV
LG
>Mature_482_residues
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYDGDFIIRIEDTDVERNVEGGIDSQFNFLE*MGIVADESIRNPKTFGP
YIQSQKLKHYEALALDLVAQKKAYFCFCSKERLDADRELAEKLHETPKYKRHCLNLNEQTIQANLLANKEYTIRLKIDEN
NEYS*DDLIRGKISIPGSALTDPVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDLSKIKYG
HLSIIVDETGKKLSKRNLALKQFVSDYEKDGY*PHAITNFVALLG*SPKNNQEIMSLVEMVENFDVNNLSKSPAFFDINK
MN*FSTQYFNNISQDEFIDFVKTHPLTKELVLKDTTFIDKALLFKSHIVNLKQLINLVDEQFNSNKQLLEEDVNHIKNNQ
LTNVVQVFYEQLIVSEKFDEQSIKEVIKQVQKTTNNKGANLYMPIRIATTFSSHGPELAKTIYYLGRENVLKNLQSILKV
LG

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=496, Percent_Identity=29.8387096774194, Blast_Score=223, Evalue=4e-58,
Organism=Escherichia coli, GI1788743, Length=494, Percent_Identity=31.1740890688259, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI226510925, Length=255, Percent_Identity=30.5882352941176, Blast_Score=102, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71993394, Length=495, Percent_Identity=34.3434343434343, Blast_Score=221, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6324540, Length=340, Percent_Identity=36.1764705882353, Blast_Score=196, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6324742, Length=315, Percent_Identity=25.3968253968254, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321192, Length=321, Percent_Identity=25.8566978193146, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=30.7228915662651, Blast_Score=218, Evalue=7e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54572; Mature: 54572

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYDGDFIIRIEDTDVERNVEGGIDSQFNF
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
LEMGIVADESIRNPKTFGPYIQSQKLKHYEALALDLVAQKKAYFCFCSKERLDADRELAE
EEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCCHHHHHH
KLHETPKYKRHCLNLNEQTIQANLLANKEYTIRLKIDENNEYSDDLIRGKISIPGSALTD
HHHHCHHHHHHHCCCCHHHEEEEEEECCEEEEEEEECCCCCCCCHHHEEEEECCCCCCCC
PVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDLSKIKYGHL
CEEEECCEEEEEEEEEEEECHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCHHHEEECEE
SIIVDETGKKLSKRNLALKQFVSDYEKDGYPHAITNFVALLGSPKNNQEIMSLVEMVENF
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
DVNNLSKSPAFFDINKMNFSTQYFNNISQDEFIDFVKTHPLTKELVLKDTTFIDKALLFK
CCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SHIVNLKQLINLVDEQFNSNKQLLEEDVNHIKNNQLTNVVQVFYEQLIVSEKFDEQSIKE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
VIKQVQKTTNNKGANLYMPIRIATTFSSHGPELAKTIYYLGRENVLKNLQSILKVLG
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKIRTRYAPSPTGYLHIGGARTALFNYLLAKAYDGDFIIRIEDTDVERNVEGGIDSQFNF
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCC
LEMGIVADESIRNPKTFGPYIQSQKLKHYEALALDLVAQKKAYFCFCSKERLDADRELAE
EEECEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHCCCHHHHHH
KLHETPKYKRHCLNLNEQTIQANLLANKEYTIRLKIDENNEYSDDLIRGKISIPGSALTD
HHHHCHHHHHHHCCCCHHHEEEEEEECCEEEEEEEECCCCCCCCHHHEEEEECCCCCCCC
PVILKSNKIAMYNFAVVIDDYEMQISHVIRGEEHISNTPYQLAIAQALNYDLSKIKYGHL
CEEEECCEEEEEEEEEEEECHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCHHHEEECEE
SIIVDETGKKLSKRNLALKQFVSDYEKDGYPHAITNFVALLGSPKNNQEIMSLVEMVENF
EEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
DVNNLSKSPAFFDINKMNFSTQYFNNISQDEFIDFVKTHPLTKELVLKDTTFIDKALLFK
CCCCCCCCCCEEEEECCCCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
SHIVNLKQLINLVDEQFNSNKQLLEEDVNHIKNNQLTNVVQVFYEQLIVSEKFDEQSIKE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHH
VIKQVQKTTNNKGANLYMPIRIATTFSSHGPELAKTIYYLGRENVLKNLQSILKVLG
HHHHHHHHCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA