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The map label for this gene is thiI

Identifier: 209554375

GI number: 209554375

Start: 514686

End: 515864

Strand: Reverse

Name: thiI

Synonym: UUR10_0463

Alternate gene names: 209554375

Gene position: 515864-514686 (Counterclockwise)

Preceding gene: 209554245

Following gene: 209554609

Centisome position: 58.99

GC content: 27.57

Gene sequence:

>1179_bases
ATGAAACCAATAATTTATATTAAATATGGCGAATTAACACTAAAGGGTAAAAATCGTGCCCAATTTATTAAAGTTTTAGT
ACACAACATTAAACAAATGCTATTAGAATATCACGAATTAGTGTACCAAGTTGGTTATGATAATTTAAAAATTATTAACT
TAGAAAAATATAACTTACAACAAGTTATTAATGATTTACAACAAGTTTATGGAATTGCTTTTATTTGCGTTGCTTATCAA
GTCAATAAAGAAATTAATGAAATTCAATTAGCATGCAATAAACTTGTTAATAATACTGATCAAACTTTTAAAATTGAAGC
TCGTCGAAATGATAAAAGTTTTATATATGATTCAATGCAAATCAAACAAATTTGTGCTACTTATTTATTACAAAACCAAC
CTACACTAAAAGTTGATGTTCATCATCCGCAATTGTTAATTAATATTGAAATTAAACATGATTGTGCTATTGTTTATGGT
CATAAAATTCCCGGTGCTAAAGGTCTACCAGTTGGTATTAATGGTAAAGCCTTAGTACTATTATCAGGTGGTATTGACTC
TCCTGTTGCTTCGCGATTAATTATGAAGCGAGGTATTAGTGTTGATTTTATTACTTTTATTACCCCACCACACACTTCAC
AAAAAGCTTTAGATAAAACAATTGCATTAGCAAAACAAATAACATTAAATAATCATTTAACTAAAGCGAATTTATATGTA
TGCAATTTCACTAAATTACAAGAAGAAATTGCTCATATTTCTAAAGAATCTTATCGAATTACGCTTATGCGTCGTTATTT
TATGCGGATTGCTAAGCGTTTAGCAATAGATATTAAAGCAGGTGCTTTAGTAACGGGTGAAGCACTTGGGCAAGTAGCTT
CACAAACTTTAAATAGTATGCAAACTATTAGTAGTGTTTTAAATGATTTTTTAGTTTTAAGACCTTTAATTACTTATGAT
AAACAAGAAATTATTAGCTTAGCTAAACAATTTAATACTTATGAATTATCAATTTTACCATATGATGATTCATGTAGTTT
GTTTGCACCTAAAAATCCAACAACTAATCCAAATGTTCAAACAGCTATGAAACTAGAGCAAGAGTCGTTGGTTTTAGATG
CGATTTATGAATTAGTTTTTACTAAAGAAATTACTAAAATTAATTTATCATCAAAATAG

Upstream 100 bases:

>100_bases
TCAAAAGCAAAAACTTGGTATGAAGAATACATTATTAAAGGGATTAGCCCTAAATTAAGCAAAGATGATCTTGAATGATA
TAAGGTATGAGTTAATAAAT

Downstream 100 bases:

>100_bases
GAGAGTTATTATGCAAAAAGATTTGTTAAATAAGTTAATTGAACAAACATATGGTTTTAGTAAAATTAGTATTTTTGTTC
ATACAAATCCCGATTGTGAT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MKPIIYIKYGELTLKGKNRAQFIKVLVHNIKQMLLEYHELVYQVGYDNLKIINLEKYNLQQVINDLQQVYGIAFICVAYQ
VNKEINEIQLACNKLVNNTDQTFKIEARRNDKSFIYDSMQIKQICATYLLQNQPTLKVDVHHPQLLINIEIKHDCAIVYG
HKIPGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISVDFITFITPPHTSQKALDKTIALAKQITLNNHLTKANLYV
CNFTKLQEEIAHISKESYRITLMRRYFMRIAKRLAIDIKAGALVTGEALGQVASQTLNSMQTISSVLNDFLVLRPLITYD
KQEIISLAKQFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAMKLEQESLVLDAIYELVFTKEITKINLSSK

Sequences:

>Translated_392_residues
MKPIIYIKYGELTLKGKNRAQFIKVLVHNIKQMLLEYHELVYQVGYDNLKIINLEKYNLQQVINDLQQVYGIAFICVAYQ
VNKEINEIQLACNKLVNNTDQTFKIEARRNDKSFIYDSMQIKQICATYLLQNQPTLKVDVHHPQLLINIEIKHDCAIVYG
HKIPGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISVDFITFITPPHTSQKALDKTIALAKQITLNNHLTKANLYV
CNFTKLQEEIAHISKESYRITLMRRYFMRIAKRLAIDIKAGALVTGEALGQVASQTLNSMQTISSVLNDFLVLRPLITYD
KQEIISLAKQFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAMKLEQESLVLDAIYELVFTKEITKINLSSK
>Mature_392_residues
MKPIIYIKYGELTLKGKNRAQFIKVLVHNIKQMLLEYHELVYQVGYDNLKIINLEKYNLQQVINDLQQVYGIAFICVAYQ
VNKEINEIQLACNKLVNNTDQTFKIEARRNDKSFIYDSMQIKQICATYLLQNQPTLKVDVHHPQLLINIEIKHDCAIVYG
HKIPGAKGLPVGINGKALVLLSGGIDSPVASRLIMKRGISVDFITFITPPHTSQKALDKTIALAKQITLNNHLTKANLYV
CNFTKLQEEIAHISKESYRITLMRRYFMRIAKRLAIDIKAGALVTGEALGQVASQTLNSMQTISSVLNDFLVLRPLITYD
KQEIISLAKQFNTYELSILPYDDSCSLFAPKNPTTNPNVQTAMKLEQESLVLDAIYELVFTKEITKINLSSK

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=391, Percent_Identity=28.9002557544757, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_UREU1 (B5ZBR5)

Other databases:

- EMBL:   CP001184
- RefSeq:   YP_002284855.1
- ProteinModelPortal:   B5ZBR5
- SMR:   B5ZBR5
- GeneID:   6984292
- GenomeReviews:   CP001184_GR
- KEGG:   uue:UUR10_0463
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   UURE95664:UUR10_0463-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 44449; Mature: 44449

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS51165 THUMP

Important sites: BINDING 264-264 BINDING 286-286 BINDING 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIIYIKYGELTLKGKNRAQFIKVLVHNIKQMLLEYHELVYQVGYDNLKIINLEKYNLQ
CCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
QVINDLQQVYGIAFICVAYQVNKEINEIQLACNKLVNNTDQTFKIEARRNDKSFIYDSMQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHEECHHH
IKQICATYLLQNQPTLKVDVHHPQLLINIEIKHDCAIVYGHKIPGAKGLPVGINGKALVL
HHHHHHHHHHCCCCCEEEEECCCEEEEEEEEECCEEEEEECCCCCCCCCEECCCCCEEEE
LSGGIDSPVASRLIMKRGISVDFITFITPPHTSQKALDKTIALAKQITLNNHLTKANLYV
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
CNFTKLQEEIAHISKESYRITLMRRYFMRIAKRLAIDIKAGALVTGEALGQVASQTLNSM
EEHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHH
QTISSVLNDFLVLRPLITYDKQEIISLAKQFNTYELSILPYDDSCSLFAPKNPTTNPNVQ
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCEECCCCCCCCCCHH
TAMKLEQESLVLDAIYELVFTKEITKINLSSK
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC
>Mature Secondary Structure
MKPIIYIKYGELTLKGKNRAQFIKVLVHNIKQMLLEYHELVYQVGYDNLKIINLEKYNLQ
CCCEEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
QVINDLQQVYGIAFICVAYQVNKEINEIQLACNKLVNNTDQTFKIEARRNDKSFIYDSMQ
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHEECHHH
IKQICATYLLQNQPTLKVDVHHPQLLINIEIKHDCAIVYGHKIPGAKGLPVGINGKALVL
HHHHHHHHHHCCCCCEEEEECCCEEEEEEEEECCEEEEEECCCCCCCCCEECCCCCEEEE
LSGGIDSPVASRLIMKRGISVDFITFITPPHTSQKALDKTIALAKQITLNNHLTKANLYV
EECCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEE
CNFTKLQEEIAHISKESYRITLMRRYFMRIAKRLAIDIKAGALVTGEALGQVASQTLNSM
EEHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHHHHH
QTISSVLNDFLVLRPLITYDKQEIISLAKQFNTYELSILPYDDSCSLFAPKNPTTNPNVQ
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCCCEECCCCCCCCCCHH
TAMKLEQESLVLDAIYELVFTKEITKINLSSK
HHHHHHHHHHHHHHHHHHHHHHHHEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA