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The map label for this gene is lepA [H]

Identifier: 209554291

GI number: 209554291

Start: 369550

End: 371349

Strand: Reverse

Name: lepA [H]

Synonym: UUR10_0331

Alternate gene names: 209554291

Gene position: 371349-369550 (Counterclockwise)

Preceding gene: 209554228

Following gene: 209554041

Centisome position: 42.47

GC content: 30.44

Gene sequence:

>1800_bases
ATGGACAAAAAGTTCATCCGTAATTTTAGTATCATCGCCCATATTGATCATGGTAAATCAACATTATCAGATCGAATTAT
TGAATTTACAAATACTTTAAGTAAACGTGAAATGACAAACCAAATTTTAGATTCTATGGACATTGAACGTGAACGTGGAA
TTACTATCAAATTAAACGCTGTGCAAATTAAATATCATGCTAGAGATAATAACGAATATTTAATTCATTTAATCGATACT
CCAGGACACGTTGATTTTACTTATGAAGTTTCGCGAAGTTTAGCTGCTTGTGAGGGTGCAATTTTAGTTGTAGATGCTGC
ACAAGGAATTGAAGCTCAAACGCTATCTAATGTTTATTTAGCATTAGAAAACAATTTAGAAATTGTGCCAACCATTAATA
AAATTGATTTACCATCAGCAGATCCTGAACGTGTTAAAAAAGAAATTGAAGATGTTATTGGTTTAGATACATCAGATATT
CCTTTAATTTCAGCAAAAACAGGTTTAAACATTCAAGATGTACTAGAAGCAATTATTAAACATGTACCACCACCATTAGA
TGCTAATGATGATGCTAAATTACAAGCCTTAATCTTTGATTCTTTCTACGATTCTTACAAAGGTGTTGTTTGTTTAGTTC
GTATAAAACAAGGAACAATTAAAGTTGGTGATAAAATTCGTATGATGGCCAACAATAAAGACTATATTGTTAGCGAATTA
GGAATTAGAACACCAAAAATTGTTAATAAAACTGAATTAGTAGCTGGAGAAGTTGGATGAGTAGCTGCTGCTATTAAAAC
AGTTAAAGATATTAATGTTGGGGATACAATTACCCATGCTAATAACCCTGCTGATAAACCATTGCCAGGTTATAAAAAAA
TCTTACCAATGGTTTATTGTGGTCTATATCCAATTGATACTAGCCAATACGATGATTTAAAAGAAGCAATGGCTAAAATT
TCATTATCAGATGCTGCATTAACTTATGAATATGAAACATCACAAGCGCTAGGATTTGGAATTCGTTGTGGATTTTTAGG
ATTATTACATATGGATGTAATTCGTGAACGAATTGCACGTGAATTTAATATTGAATTAATCTTAACTGCACCTTCAGTAA
TTTATAAAATTGAGTTAACAAATAATCAAGAAATTAGTATCGATAGTCCTGCTAAAATGCCAGAACCAACTAATATTAAA
GCAATTAAAGAACCATTTGTTAAATTAGCAATCATTACACCAGATAATTATGTTGGTGCTATTATGGAATTGTGTCAATC
ACGAAGAGGATCTTACCAAGATTTAGAAGTTATTGATGGTACAAGACGACGATTAATTTATAAAATGCCATTAGCTGAAA
TTATGTATAGTTTTTTTGATTCACTAAAATCAATTACTAAAGGATATGCAACCATGGATTACGAACTAATTGGCTATCAA
GCTGAAAAATTAGTTAAAATTGATATTATGTTAAATGGTAATAAAGTTGATGCTTTAAGTATTATTGCACATCGTGATTT
TGCTTATGGTAAATCAAAAATTATTTGCGAAAGATTAAAAGAAGTGATTCCAAAACACCAATTTGAAATTCCTATCCAAG
CTTCAATTGGATCAAAAATTATTGCTCGTGAAACTATTAAAGCAGTACGTAAGGACGTAATTGCTAAATGTTATGGTGGA
GATGTTTCAAGAAAGAAAAAACTTCTAGAACAACAAAAAGAAGGTAAAAAACGTCTAAAAGCCATTGGAAACGTTGATGT
TCCACAAGATGCCTTTGTTAAAGTTTTAAGTGAAAACTAA

Upstream 100 bases:

>100_bases
TATGTAACAACTCTTTCTTGGAGTTGTTTTTTTATTTTTATTACTTTAAAATAATTTAAAATATAATAATGTTTTTAAAA
AAGTAAAGAGTGGTTATTAT

Downstream 100 bases:

>100_bases
TACCAATTATTTATGTTGAATTTTGTCAAAGATCTAAATTTTGAAATTAATCAAAAGAAGATTATTGTTAAAGTTTGTTT
ACAACCAAAAATTAAATACA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNAVQIKYHARDNNEYLIHLIDT
PGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYLALENNLEIVPTINKIDLPSADPERVKKEIEDVIGLDTSDI
PLISAKTGLNIQDVLEAIIKHVPPPLDANDDAKLQALIFDSFYDSYKGVVCLVRIKQGTIKVGDKIRMMANNKDYIVSEL
GIRTPKIVNKTELVAGEVGWVAAAIKTVKDINVGDTITHANNPADKPLPGYKKILPMVYCGLYPIDTSQYDDLKEAMAKI
SLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIAREFNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTNIK
AIKEPFVKLAIITPDNYVGAIMELCQSRRGSYQDLEVIDGTRRRLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQ
AEKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKIIARETIKAVRKDVIAKCYGG
DVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN

Sequences:

>Translated_599_residues
MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNAVQIKYHARDNNEYLIHLIDT
PGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYLALENNLEIVPTINKIDLPSADPERVKKEIEDVIGLDTSDI
PLISAKTGLNIQDVLEAIIKHVPPPLDANDDAKLQALIFDSFYDSYKGVVCLVRIKQGTIKVGDKIRMMANNKDYIVSEL
GIRTPKIVNKTELVAGEVG*VAAAIKTVKDINVGDTITHANNPADKPLPGYKKILPMVYCGLYPIDTSQYDDLKEAMAKI
SLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIAREFNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTNIK
AIKEPFVKLAIITPDNYVGAIMELCQSRRGSYQDLEVIDGTRRRLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQ
AEKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKIIARETIKAVRKDVIAKCYGG
DVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN
>Mature_599_residues
MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNAVQIKYHARDNNEYLIHLIDT
PGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYLALENNLEIVPTINKIDLPSADPERVKKEIEDVIGLDTSDI
PLISAKTGLNIQDVLEAIIKHVPPPLDANDDAKLQALIFDSFYDSYKGVVCLVRIKQGTIKVGDKIRMMANNKDYIVSEL
GIRTPKIVNKTELVAGEVG*VAAAIKTVKDINVGDTITHANNPADKPLPGYKKILPMVYCGLYPIDTSQYDDLKEAMAKI
SLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIAREFNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTNIK
AIKEPFVKLAIITPDNYVGAIMELCQSRRGSYQDLEVIDGTRRRLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQ
AEKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKIIARETIKAVRKDVIAKCYGG
DVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=599, Percent_Identity=50.0834724540901, Blast_Score=610, Evalue=1e-175,
Organism=Homo sapiens, GI94966754, Length=132, Percent_Identity=46.2121212121212, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=38.6666666666667, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=39.5973154362416, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=44.0298507462687, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=44.0298507462687, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=44.0298507462687, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=44.0366972477064, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=44.0366972477064, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=44.0366972477064, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI53729339, Length=246, Percent_Identity=28.8617886178862, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI53729337, Length=246, Percent_Identity=28.8617886178862, Blast_Score=88, Evalue=3e-17,
Organism=Homo sapiens, GI217272894, Length=176, Percent_Identity=32.3863636363636, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI217272892, Length=176, Percent_Identity=32.3863636363636, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI34147630, Length=257, Percent_Identity=28.7937743190661, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI94966752, Length=97, Percent_Identity=41.2371134020619, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI223555963, Length=283, Percent_Identity=24.7349823321555, Blast_Score=70, Evalue=8e-12,
Organism=Homo sapiens, GI5729864, Length=283, Percent_Identity=24.7349823321555, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=593, Percent_Identity=54.8060708263069, Blast_Score=676, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=509, Percent_Identity=29.0766208251473, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1790835, Length=161, Percent_Identity=31.6770186335404, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789559, Length=253, Percent_Identity=31.2252964426877, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1789738, Length=161, Percent_Identity=34.7826086956522, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1789108, Length=362, Percent_Identity=25.414364640884, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789737, Length=240, Percent_Identity=28.75, Blast_Score=70, Evalue=6e-13,
Organism=Escherichia coli, GI1790412, Length=240, Percent_Identity=28.75, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17557151, Length=610, Percent_Identity=41.3114754098361, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17556745, Length=472, Percent_Identity=23.5169491525424, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=143, Percent_Identity=37.0629370629371, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=158, Percent_Identity=33.5443037974684, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=36.0902255639098, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=35.5555555555556, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17552882, Length=177, Percent_Identity=31.0734463276836, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994658, Length=222, Percent_Identity=27.4774774774775, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556456, Length=249, Percent_Identity=30.5220883534137, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=246, Percent_Identity=29.2682926829268, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=42.6421404682274, Blast_Score=494, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6323098, Length=181, Percent_Identity=34.8066298342541, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=39.5973154362416, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=39.5973154362416, Blast_Score=103, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=113, Percent_Identity=41.5929203539823, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=35.8620689655172, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=248, Percent_Identity=29.0322580645161, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI78706572, Length=599, Percent_Identity=44.5742904841402, Blast_Score=521, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24582462, Length=163, Percent_Identity=38.0368098159509, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=40.2684563758389, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI28574573, Length=140, Percent_Identity=40.7142857142857, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=35.2941176470588, Blast_Score=94, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21357743, Length=163, Percent_Identity=32.5153374233129, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI28572034, Length=269, Percent_Identity=27.8810408921933, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=248, Percent_Identity=28.6290322580645, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17864358, Length=248, Percent_Identity=28.6290322580645, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921738, Length=250, Percent_Identity=29.2, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66846; Mature: 66846

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNA
CCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEE
VQIKYHARDNNEYLIHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYL
EEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHCEEEE
ALENNLEIVPTINKIDLPSADPERVKKEIEDVIGLDTSDIPLISAKTGLNIQDVLEAIIK
EEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHH
HVPPPLDANDDAKLQALIFDSFYDSYKGVVCLVRIKQGTIKVGDKIRMMANNKDYIVSEL
HCCCCCCCCCCCEEEEEEHHHHHHCCCCEEEEEEEECCCEEECCEEEEEECCCCEEEHHC
GIRTPKIVNKTELVAGEVGVAAAIKTVKDINVGDTITHANNPADKPLPGYKKILPMVYCG
CCCCCCCCCHHHHEECCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHC
LYPIDTSQYDDLKEAMAKISLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIARE
CEECCCCCHHHHHHHHHHCEECCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHH
FNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTNIKAIKEPFVKLAIITPDNYVGAI
CCEEEEEECCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHH
MELCQSRRGSYQDLEVIDGTRRRLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQA
HHHHHHHCCCCCCHHHHCCCHHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
EKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKII
CEEEEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCHHH
ARETIKAVRKDVIAKCYGGDVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MDKKFIRNFSIIAHIDHGKSTLSDRIIEFTNTLSKREMTNQILDSMDIERERGITIKLNA
CCCHHHCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCEEEEEEE
VQIKYHARDNNEYLIHLIDTPGHVDFTYEVSRSLAACEGAILVVDAAQGIEAQTLSNVYL
EEEEEEECCCCCEEEEEECCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCHHHHCEEEE
ALENNLEIVPTINKIDLPSADPERVKKEIEDVIGLDTSDIPLISAKTGLNIQDVLEAIIK
EEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHH
HVPPPLDANDDAKLQALIFDSFYDSYKGVVCLVRIKQGTIKVGDKIRMMANNKDYIVSEL
HCCCCCCCCCCCEEEEEEHHHHHHCCCCEEEEEEEECCCEEECCEEEEEECCCCEEEHHC
GIRTPKIVNKTELVAGEVGVAAAIKTVKDINVGDTITHANNPADKPLPGYKKILPMVYCG
CCCCCCCCCHHHHEECCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHC
LYPIDTSQYDDLKEAMAKISLSDAALTYEYETSQALGFGIRCGFLGLLHMDVIRERIARE
CEECCCCCHHHHHHHHHHCEECCCEEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHHH
FNIELILTAPSVIYKIELTNNQEISIDSPAKMPEPTNIKAIKEPFVKLAIITPDNYVGAI
CCEEEEEECCCEEEEEEECCCCEEECCCCCCCCCCCCCHHHCCCCEEEEEECCCHHHHHH
MELCQSRRGSYQDLEVIDGTRRRLIYKMPLAEIMYSFFDSLKSITKGYATMDYELIGYQA
HHHHHHHCCCCCCHHHHCCCHHHEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCC
EKLVKIDIMLNGNKVDALSIIAHRDFAYGKSKIICERLKEVIPKHQFEIPIQASIGSKII
CEEEEEEEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCHHH
ARETIKAVRKDVIAKCYGGDVSRKKKLLEQQKEGKKRLKAIGNVDVPQDAFVKVLSEN
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA