Click here to switch to the map view.

The map label for this gene is proS [H]

Identifier: 209554280

GI number: 209554280

Start: 536688

End: 538106

Strand: Reverse

Name: proS [H]

Synonym: UUR10_0487

Alternate gene names: 209554280

Gene position: 538106-536688 (Counterclockwise)

Preceding gene: 209554191

Following gene: 209554048

Centisome position: 61.53

GC content: 28.61

Gene sequence:

>1419_bases
ATGGCTAAGAAATTAGAAAAAATTATAACAAGAAACGAGAATTTTGCTGATTGATACACAAGTATTGTTAATAATGCAAA
ATTAATTCAATATACAGATATTAAGGGAATGATGGTTTTCCAACCAAATGCTTGAGCAATTTGAGAAGCTATTAAAAATC
AAATTGATCTTGAATTTAAAAAACATGGCGTTCGCAATCTTGCTATGCCAACTTTAATTCCATTAAGTGAATTTCAAAAA
GAAAAAGATCATATTGAAGGTTTTGCACCTGAATTGTTTATGGTAAACCAAATTGGTGATAAAAAGCTTGACAATCCCTA
TGCTATACGCCCCACAAGTGAAATTTTATTTTGTAACTATTTTAAAAATATTGTTAATAGTTATAATGACTTACCTATTA
AAAACAACCAATGATGTTCAGTGATGCGTGCTGAAAAAACAACACGTCCATTCTTAAGAAATGCTGAATTTCATTGACAA
GAGTTACATGCTATTTTTGCAAGTGAACATGAAGCTGATGAGTTTGCTAAAACAATTTTAGATGTATACACTGATTTTGT
TCAAAACTATTTATGCATTCCTGTTATTAAAGGTTTAAAAACACCATGAGAACGTTTTGCCGGCGCTCAAAAAACATATA
CAATCGAAGCAATGATGCAAGATGGGCAAGCCTTGCAATCAGCAACAAGTCATTATCTAGGCCAATTTTTTGCTAAAGCT
TATGATATTAAATTTCAAGGCCAAGATAATCAAATGCATTATGTACATCAAATGTCAGCTGGTTTATCAACACGTATTAT
TGGTGCTTTAATTATGGTTCATGCTGATGATCAAGGTTTAATTTTACCTCCAGATATTGCTTTTAACCAAATCGCTATTT
TATCGATCTTTGCTAATAAAAATCCACAATTATTAACAATTAGTGAACAAATTCGTAATGAATTAAGTGATTATCGTTTA
TTTGAAGATCATAGTGATAAAGGTGTAGGCTATAAATTAGCACAACAAGAAATTGAGGGAACACCAATTTGTATTTTAGT
TGGTGTTAAAGAACTTGCTAACCAACAAGTTGTACTTGTTCGTCGTGATACACATGAAAAAATTAATGTTAATTTAATTG
ATTTAAAATCAACTATTAAAAAATTATTGCTAGATATTAAAACAAATATTTATCAAAAAGCAAAAAAACAATTAGATGAA
TCAATTGTTTTTGTAAATAGTATTGAAGAACTAAAACAAGTAATTGCTCAAAATAAAATGGCTAAAGCTTTTTTTGATGG
TTCAAAAGAAGATGATGAACAAATTAAATTATTAACAAATGCATCAACTCGTTGTATTTTTGATGAAACTCAAAGTGGAC
AATGTTTTTATACAAATAAAAAAACAAACAAATTAACTTTATTCGCACGAGCTTATTAA

Upstream 100 bases:

>100_bases
AATAAACGCTTTAAAAACATGTTTAGAGCAATATAAACAATAAAATTACATTAAATATTTATTAAAATTAAATATAAAAA
AGTAGGAATAAATAGATAAA

Downstream 100 bases:

>100_bases
CATTAATAAAATAGATTAAAAAAGTTTATTTCATATATAATTTAAATTAATTATAGAAGTTTAAAATTTAAAATTAGAAA
ACAATTAATTTTTGGAGTTA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAKKLEKIITRNENFADWYTSIVNNAKLIQYTDIKGMMVFQPNAWAIWEAIKNQIDLEFKKHGVRNLAMPTLIPLSEFQK
EKDHIEGFAPELFMVNQIGDKKLDNPYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQWCSVMRAEKTTRPFLRNAEFHWQ
ELHAIFASEHEADEFAKTILDVYTDFVQNYLCIPVIKGLKTPWERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKA
YDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILSIFANKNPQLLTISEQIRNELSDYRL
FEDHSDKGVGYKLAQQEIEGTPICILVGVKELANQQVVLVRRDTHEKINVNLIDLKSTIKKLLLDIKTNIYQKAKKQLDE
SIVFVNSIEELKQVIAQNKMAKAFFDGSKEDDEQIKLLTNASTRCIFDETQSGQCFYTNKKTNKLTLFARAY

Sequences:

>Translated_472_residues
MAKKLEKIITRNENFAD*YTSIVNNAKLIQYTDIKGMMVFQPNA*AI*EAIKNQIDLEFKKHGVRNLAMPTLIPLSEFQK
EKDHIEGFAPELFMVNQIGDKKLDNPYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQ*CSVMRAEKTTRPFLRNAEFH*Q
ELHAIFASEHEADEFAKTILDVYTDFVQNYLCIPVIKGLKTP*ERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKA
YDIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILSIFANKNPQLLTISEQIRNELSDYRL
FEDHSDKGVGYKLAQQEIEGTPICILVGVKELANQQVVLVRRDTHEKINVNLIDLKSTIKKLLLDIKTNIYQKAKKQLDE
SIVFVNSIEELKQVIAQNKMAKAFFDGSKEDDEQIKLLTNASTRCIFDETQSGQCFYTNKKTNKLTLFARAY
>Mature_471_residues
AKKLEKIITRNENFAD*YTSIVNNAKLIQYTDIKGMMVFQPNA*AI*EAIKNQIDLEFKKHGVRNLAMPTLIPLSEFQKE
KDHIEGFAPELFMVNQIGDKKLDNPYAIRPTSEILFCNYFKNIVNSYNDLPIKNNQ*CSVMRAEKTTRPFLRNAEFH*QE
LHAIFASEHEADEFAKTILDVYTDFVQNYLCIPVIKGLKTP*ERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAY
DIKFQGQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILSIFANKNPQLLTISEQIRNELSDYRLF
EDHSDKGVGYKLAQQEIEGTPICILVGVKELANQQVVLVRRDTHEKINVNLIDLKSTIKKLLLDIKTNIYQKAKKQLDES
IVFVNSIEELKQVIAQNKMAKAFFDGSKEDDEQIKLLTNASTRCIFDETQSGQCFYTNKKTNKLTLFARAY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro) [H]

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62241042, Length=501, Percent_Identity=35.7285429141717, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71991342, Length=490, Percent_Identity=35.1020408163265, Blast_Score=274, Evalue=6e-74,
Organism=Caenorhabditis elegans, GI71991346, Length=358, Percent_Identity=37.7094972067039, Blast_Score=237, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321808, Length=432, Percent_Identity=36.1111111111111, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24649466, Length=496, Percent_Identity=35.8870967741936, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24649468, Length=496, Percent_Identity=35.8870967741936, Blast_Score=260, Evalue=2e-69,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 53096; Mature: 52965

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKLEKIITRNENFADYTSIVNNAKLIQYTDIKGMMVFQPNAAIEAIKNQIDLEFKKHG
CCHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHCHHHHHCC
VRNLAMPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNPYAIRPTSEILFCNYFKN
CHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHH
IVNSYNDLPIKNNQCSVMRAEKTTRPFLRNAEFHQELHAIFASEHEADEFAKTILDVYTD
HHCCCCCCCCCCCCCCEEECCHHCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FVQNYLCIPVIKGLKTPERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAYDIKFQ
HHHCCCHHHHHHCCCCHHHHCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEC
GQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILSIFANKNPQLLT
CCCCCCHHHHHHHCCHHHHHEEEEEEEEECCCCCEECCCCCCCCEEEEEEEECCCCCEEE
ISEQIRNELSDYRLFEDHSDKGVGYKLAQQEIEGTPICILVGVKELANQQVVLVRRDTHE
EHHHHHHHHHHCEECCCCCCCCCCHHHHHHHCCCCCEEEEEECHHHCCCEEEEEEECCCC
KINVNLIDLKSTIKKLLLDIKTNIYQKAKKQLDESIVFVNSIEELKQVIAQNKMAKAFFD
EEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCC
GSKEDDEQIKLLTNASTRCIFDETQSGQCFYTNKKTNKLTLFARAY
CCCCCCCCEEEEECCCCEEEEECCCCCCEEEECCCCCEEEEEEECC
>Mature Secondary Structure 
AKKLEKIITRNENFADYTSIVNNAKLIQYTDIKGMMVFQPNAAIEAIKNQIDLEFKKHG
CHHHHHHHHCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHCHHHHHCC
VRNLAMPTLIPLSEFQKEKDHIEGFAPELFMVNQIGDKKLDNPYAIRPTSEILFCNYFKN
CHHCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHH
IVNSYNDLPIKNNQCSVMRAEKTTRPFLRNAEFHQELHAIFASEHEADEFAKTILDVYTD
HHCCCCCCCCCCCCCCEEECCHHCCHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FVQNYLCIPVIKGLKTPERFAGAQKTYTIEAMMQDGQALQSATSHYLGQFFAKAYDIKFQ
HHHCCCHHHHHHCCCCHHHHCCCCCEEEHHHHHHCCHHHHHHHHHHHHHHHHHHEEEEEC
GQDNQMHYVHQMSAGLSTRIIGALIMVHADDQGLILPPDIAFNQIAILSIFANKNPQLLT
CCCCCCHHHHHHHCCHHHHHEEEEEEEEECCCCCEECCCCCCCCEEEEEEEECCCCCEEE
ISEQIRNELSDYRLFEDHSDKGVGYKLAQQEIEGTPICILVGVKELANQQVVLVRRDTHE
EHHHHHHHHHHCEECCCCCCCCCCHHHHHHHCCCCCEEEEEECHHHCCCEEEEEEECCCC
KINVNLIDLKSTIKKLLLDIKTNIYQKAKKQLDESIVFVNSIEELKQVIAQNKMAKAFFD
EEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHCC
GSKEDDEQIKLLTNASTRCIFDETQSGQCFYTNKKTNKLTLFARAY
CCCCCCCCEEEEECCCCEEEEECCCCCCEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA