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The map label for this gene is dnaN [H]

Identifier: 209551896

GI number: 209551896

Start: 4494050

End: 4495168

Strand: Reverse

Name: dnaN [H]

Synonym: Rleg2_4329

Alternate gene names: 209551896

Gene position: 4495168-4494050 (Counterclockwise)

Preceding gene: 209551900

Following gene: 209551895

Centisome position: 99.06

GC content: 61.48

Gene sequence:

>1119_bases
ATGCGTATTACTATTGAGCGGTCAAACCTGTTGAAATCGCTGAACCACGTGCACCGCGTGGTCGAACGTCGCAACACGAT
CCCGATCCTGTCCAACGTATTGCTCAAGGCCGACGGCCAGAACCTCGACATGAAGGCGACCGACCTCGACCTCGAAATCA
CCGAGGCGACGCCGGCAAGCGTCGAACAATCAGGCGCCACCACCGTTCCGGCCCATCTTCTCTACGATATCGTGCGCAAG
CTTCCCGACGGCTCGGAAGTGCTACTGGCCACCAGCAGCGATGGCGGCTCGATGACCGTGCAGTCCGGCCGCTCGAAATT
CTCGCTGCAGTGCCTGCCGGAATCCGACTTTCCCGATCTGACCGCTGGCACCTTCACGCATTCCTTCAAGCTGAAGGCGA
CCGATCTGAAAATGCTGATCGACCGCACGCAGTTTGCGATCTCGACCGAGGAGACGCGCTATTATCTCAACGGCATCTTT
TTCCACACGATCGAGAGCAAGGGCGAGCTGAAGCTCCGGGCAGTCGCGACCGACGGCCACCGGCTGGCGCGCGCCGATGT
CGATGCGCCCTCCGGCTCCGAAGGCATGCCCGGCATCATCATTCCGCGCAAGACTGTGGGAGAATTGCAGAAGCTGGTCG
ACAATCCGGAGGTGACGGTGACCGTCGAGGTCTCCGACGCCAAGATCCGCCTGACGATCGGCTCGATCGTCATGACCTCG
AAACTGATCGACGGCACCTTCCCGGATTACCAGCGGGTCATCCCGACCGGCAACGACAAGGAAATGCGGGTCGACTGCAC
GACCTTCGCCCAGGCCGTCGACCGCGTCTCCACAATTTCCTCCGAGCGCGGCCGCGCCGTGAAGCTGGCGCTGTCCGAGG
GACAGCTGATGCTGACGGTCAACAATCCGGATTCCGGCAGCGCCACGGAAGAAGTCGCCGTCGGCTACGACACGGATGCG
ATGGAAATCGGCTTCAATGCCAAATACCTGCTCGACATCACCGCGCAGCTTTCCGGCGAGGAAGCGATCTTCCTGCTTGC
CGATGCCGGTTCGCCGACGCTGATCCGTGACACGGCCGGCGACGATGCGCTTTACGTGCTGATGCCGATGCGCGTCTGA

Upstream 100 bases:

>100_bases
CTGCCATTTTGCCCGTCCACTCCCCCGGTCTCAGGCTGGGAAAGCATTCTTCGGGTGCTCGACGCGCCCGGACAGTCACG
GTCGAAAGCGGTAGCATTCC

Downstream 100 bases:

>100_bases
CGGGAACGAAGGCCATCCGTCTCCGTTTTTCCTCCTGTTAAGTGGAGGGTTGCTCCTCCTGTGAAAACGGAGGATCCGAT
GATGAAAAAGGCGGGAACGG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRITIERSNLLKSLNHVHRVVERRNTIPILSNVLLKADGQNLDMKATDLDLEITEATPASVEQSGATTVPAHLLYDIVRK
LPDGSEVLLATSSDGGSMTVQSGRSKFSLQCLPESDFPDLTAGTFTHSFKLKATDLKMLIDRTQFAISTEETRYYLNGIF
FHTIESKGELKLRAVATDGHRLARADVDAPSGSEGMPGIIIPRKTVGELQKLVDNPEVTVTVEVSDAKIRLTIGSIVMTS
KLIDGTFPDYQRVIPTGNDKEMRVDCTTFAQAVDRVSTISSERGRAVKLALSEGQLMLTVNNPDSGSATEEVAVGYDTDA
MEIGFNAKYLLDITAQLSGEEAIFLLADAGSPTLIRDTAGDDALYVLMPMRV

Sequences:

>Translated_372_residues
MRITIERSNLLKSLNHVHRVVERRNTIPILSNVLLKADGQNLDMKATDLDLEITEATPASVEQSGATTVPAHLLYDIVRK
LPDGSEVLLATSSDGGSMTVQSGRSKFSLQCLPESDFPDLTAGTFTHSFKLKATDLKMLIDRTQFAISTEETRYYLNGIF
FHTIESKGELKLRAVATDGHRLARADVDAPSGSEGMPGIIIPRKTVGELQKLVDNPEVTVTVEVSDAKIRLTIGSIVMTS
KLIDGTFPDYQRVIPTGNDKEMRVDCTTFAQAVDRVSTISSERGRAVKLALSEGQLMLTVNNPDSGSATEEVAVGYDTDA
MEIGFNAKYLLDITAQLSGEEAIFLLADAGSPTLIRDTAGDDALYVLMPMRV
>Mature_372_residues
MRITIERSNLLKSLNHVHRVVERRNTIPILSNVLLKADGQNLDMKATDLDLEITEATPASVEQSGATTVPAHLLYDIVRK
LPDGSEVLLATSSDGGSMTVQSGRSKFSLQCLPESDFPDLTAGTFTHSFKLKATDLKMLIDRTQFAISTEETRYYLNGIF
FHTIESKGELKLRAVATDGHRLARADVDAPSGSEGMPGIIIPRKTVGELQKLVDNPEVTVTVEVSDAKIRLTIGSIVMTS
KLIDGTFPDYQRVIPTGNDKEMRVDCTTFAQAVDRVSTISSERGRAVKLALSEGQLMLTVNNPDSGSATEEVAVGYDTDA
MEIGFNAKYLLDITAQLSGEEAIFLLADAGSPTLIRDTAGDDALYVLMPMRV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=41.8666666666667, Blast_Score=284, Evalue=8e-78,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40438; Mature: 40438

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00041 HTH_ARAC_FAMILY_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITIERSNLLKSLNHVHRVVERRNTIPILSNVLLKADGQNLDMKATDLDLEITEATPAS
CEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCEEEECCEEEEECCCCCC
VEQSGATTVPAHLLYDIVRKLPDGSEVLLATSSDGGSMTVQSGRSKFSLQCLPESDFPDL
HHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
TAGTFTHSFKLKATDLKMLIDRTQFAISTEETRYYLNGIFFHTIESKGELKLRAVATDGH
CCCCEEEEEEEEECHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLARADVDAPSGSEGMPGIIIPRKTVGELQKLVDNPEVTVTVEVSDAKIRLTIGSIVMTS
EEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCEEEEH
KLIDGTFPDYQRVIPTGNDKEMRVDCTTFAQAVDRVSTISSERGRAVKLALSEGQLMLTV
HHHCCCCCCHHEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
NNPDSGSATEEVAVGYDTDAMEIGFNAKYLLDITAQLSGEEAIFLLADAGSPTLIRDTAG
CCCCCCCCCCEEEEECCCCCEEECCCCEEEEEEEEECCCCEEEEEEECCCCCEEEEECCC
DDALYVLMPMRV
CCEEEEEEECCC
>Mature Secondary Structure
MRITIERSNLLKSLNHVHRVVERRNTIPILSNVLLKADGQNLDMKATDLDLEITEATPAS
CEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEECCCCCCEEEECCEEEEECCCCCC
VEQSGATTVPAHLLYDIVRKLPDGSEVLLATSSDGGSMTVQSGRSKFSLQCLPESDFPDL
HHCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCEEEEEEECCCCCCCC
TAGTFTHSFKLKATDLKMLIDRTQFAISTEETRYYLNGIFFHTIESKGELKLRAVATDGH
CCCCEEEEEEEEECHHHHHHHHHHEEEECCCCEEEEEEEEEEEECCCCCEEEEEEECCCC
RLARADVDAPSGSEGMPGIIIPRKTVGELQKLVDNPEVTVTVEVSDAKIRLTIGSIVMTS
EEEEECCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCEEEEEEECCCEEEEEECCEEEEH
KLIDGTFPDYQRVIPTGNDKEMRVDCTTFAQAVDRVSTISSERGRAVKLALSEGQLMLTV
HHHCCCCCCHHEECCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEE
NNPDSGSATEEVAVGYDTDAMEIGFNAKYLLDITAQLSGEEAIFLLADAGSPTLIRDTAG
CCCCCCCCCCEEEEECCCCCEEECCCCEEEEEEEEECCCCEEEEEEECCCCCEEEEECCC
DDALYVLMPMRV
CCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9079919; 8226640 [H]