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The map label for this gene is nodL [H]

Identifier: 209551819

GI number: 209551819

Start: 4426202

End: 4426750

Strand: Direct

Name: nodL [H]

Synonym: Rleg2_4251

Alternate gene names: 209551819

Gene position: 4426202-4426750 (Clockwise)

Preceding gene: 209551818

Following gene: 209551820

Centisome position: 97.54

GC content: 66.12

Gene sequence:

>549_bases
ATGCCGATGAGCGAACGCGAGAAAATGGCGGCAGGCGAATGGTATTGCTGCCTGGACGACGAACTCGATCTCTTGCGCCG
CCAAGCGCGCCTGGCGGTCCATGCGCACATCACGCTGCCGCCGGACGAGCGCGGCGCCATGGCGCCGGCCCTGAGGGCGC
TCTTTGCGGGCGTGGCGCCTGATGTCTTCATCGAATCGCCATTTCACTGCTCCTACGGCATCAACATCACGCTCGGCGAA
CGCGTCTATTTCAATGCCGGCTGCACCATCCTCGATTCCGGCCGGGTGATCATCGGCGACCGCAGCATGTTCGGGCCGGG
GGTGCAGATCTATTGCGCCGAGCATCACAAGGAGGCCGCACTTCGCAGCGCCGGCATCGAGATCGCCCGGCCGGTTTCGA
TCGGTAGCGATGTCTGGATCGGCGGCGGCGCCATCATCCTCGGCGGCGTCACGATCGGCAAGGGCGCGATCGTCGGTGCA
GGCGCCGTGGTGACGCGGGATGTGCCGGCCGGCGCAACAGTGGTCGGCAATCCGGCCCGGATCCGATAA

Upstream 100 bases:

>100_bases
GCCGATGCCGCGGGCCTTGCCATCGCCCGTGCCTGGGTCGGCTTGATCGAACCGGAAGAGATCCGGTTGCGGTCGCAAAT
TTCCGCTTAGGGGCGATGCG

Downstream 100 bases:

>100_bases
CGCCGGCAAGACGGCATTCCAAGCGGCCTTGCCACCTCGACAGCGGCGATCAATCCGCTAGATCATCGGCGAACGGGAGG
AAATATCATGACAGCACAGA

Product: transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 181

Protein sequence:

>182_residues
MPMSEREKMAAGEWYCCLDDELDLLRRQARLAVHAHITLPPDERGAMAPALRALFAGVAPDVFIESPFHCSYGINITLGE
RVYFNAGCTILDSGRVIIGDRSMFGPGVQIYCAEHHKEAALRSAGIEIARPVSIGSDVWIGGGAIILGGVTIGKGAIVGA
GAVVTRDVPAGATVVGNPARIR

Sequences:

>Translated_182_residues
MPMSEREKMAAGEWYCCLDDELDLLRRQARLAVHAHITLPPDERGAMAPALRALFAGVAPDVFIESPFHCSYGINITLGE
RVYFNAGCTILDSGRVIIGDRSMFGPGVQIYCAEHHKEAALRSAGIEIARPVSIGSDVWIGGGAIILGGVTIGKGAIVGA
GAVVTRDVPAGATVVGNPARIR
>Mature_181_residues
PMSEREKMAAGEWYCCLDDELDLLRRQARLAVHAHITLPPDERGAMAPALRALFAGVAPDVFIESPFHCSYGINITLGER
VYFNAGCTILDSGRVIIGDRSMFGPGVQIYCAEHHKEAALRSAGIEIARPVSIGSDVWIGGGAIILGGVTIGKGAIVGAG
AVVTRDVPAGATVVGNPARIR

Specific function: Acetyltransferase implicated in the O-acetylation of Nod factors [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=178, Percent_Identity=44.3820224719101, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1786537, Length=181, Percent_Identity=33.1491712707182, Blast_Score=113, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6322243, Length=180, Percent_Identity=38.3333333333333, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 19194; Mature: 19063

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMSEREKMAAGEWYCCLDDELDLLRRQARLAVHAHITLPPDERGAMAPALRALFAGVAP
CCCCCCHHHCCCCEEEEECCHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHCCCC
DVFIESPFHCSYGINITLGERVYFNAGCTILDSGRVIIGDRSMFGPGVQIYCAEHHKEAA
CEEECCCCCEECCEEEEECCEEEEECCCEEEECCEEEEECCCCCCCCEEEEECCCCHHHH
LRSAGIEIARPVSIGSDVWIGGGAIILGGVTIGKGAIVGAGAVVTRDVPAGATVVGNPAR
HHHCCCEEEECEECCCCEEECCCEEEEEEEEECCCEEEECCEEEEECCCCCCEEECCCCC
IR
CC
>Mature Secondary Structure 
PMSEREKMAAGEWYCCLDDELDLLRRQARLAVHAHITLPPDERGAMAPALRALFAGVAP
CCCCCHHHCCCCEEEEECCHHHHHHHHHEEEEEEEEECCCCCCCCHHHHHHHHHHCCCC
DVFIESPFHCSYGINITLGERVYFNAGCTILDSGRVIIGDRSMFGPGVQIYCAEHHKEAA
CEEECCCCCEECCEEEEECCEEEEECCCEEEECCEEEEECCCCCCCCEEEEECCCCHHHH
LRSAGIEIARPVSIGSDVWIGGGAIILGGVTIGKGAIVGAGAVVTRDVPAGATVVGNPAR
HHHCCCEEEECEECCCCEEECCCEEEEEEEEECCCEEEECCEEEEECCCCCCEEECCCCC
IR
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 62 {glucose}} 90 {maltose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1558960; 8801423; 11481432 [H]