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The map label for this gene is purU [H]

Identifier: 209551777

GI number: 209551777

Start: 4382937

End: 4383821

Strand: Reverse

Name: purU [H]

Synonym: Rleg2_4206

Alternate gene names: 209551777

Gene position: 4383821-4382937 (Counterclockwise)

Preceding gene: 209551778

Following gene: 209551776

Centisome position: 96.6

GC content: 59.1

Gene sequence:

>885_bases
ATGAAAAGCTATGTATTGACGGTATCCTGCAAGTCGACGCGCGGCATCGTTGCGGCGATTTCGAGCTATCTGGCGGAGAA
GGGCTGCAACATCATCGACTCCAGCCAGTTCGACGATCTCGATACCGGCAAGTTCTTCATGCGTGTCTCGTTCATCTCGG
AAGAAGGCCTGTCCGGCTCAGAGATCGGCGCCGATTTTGCCGCCGTCGCCGCGCCCTTTTCGATGGAATACGAATTCCAC
GACAGCGAAAGCCGTATGAAGGTGCTGCTGATGGTGTCGCGCTTCGGCCATTGTCTCAACGACCTGCTCTACCGCTGGAA
GATCGGCGCCCTGCCGATCGATATCGTCGGCGTCGTCTCCAACCATTTCGACTATCAGAAGGTCGTGGTCAACCACGACA
TTCCCTTCCACCATATTCCGGTCACCAAGGCCAACAAGGTTCAGGCCGAAGCCCATATCATGGAGGTCGCCGAGCAGACC
GGCACCGAACTGATCGTGCTCGCCCGCTACATGCAGATCCTGTCGGACGAAATGTGTCAGAAGATGTCGGGCAAGATCAT
CAACATCCACCATTCCTTCCTGCCGAGCTTCAAGGGCGCCAACCCCTACAAGCAGGCCTACGGACGCGGCGTCAAGCTGA
TCGGGGCAACCGCCCATTACGTCACCGCCGATCTCGACGAAGGCCCGATCATCGAGCAGGACACGGCCCGCATCACCCAT
GCGCAGTCGCCAGACGACTATGTCTCGATCGGCCGCGACGTCGAAAGCCAGGTGCTGGCCCGGGCCATCCACGCCCATAT
CCACCACCGCACCTTCATCAACGGCAATAGAACCGTGGTCTTCCCGGCAAGCCCCGGCAGCTACGCCTCCGAACGCATGG
GTTGA

Upstream 100 bases:

>100_bases
TCTTGCAATTTGTCGCATGAATGAAAGCGCACCGGCCTGTTCGCGGCCCAGGCTTTGATCTAAGCACGGCCGCGGCCTTG
AAGAGGGAAGAGGGAGAGAC

Downstream 100 bases:

>100_bases
ACGCGGTCGAAACCACATCTCTTTTGCTGGCAGACGACAATCTCCCGTTGCCTGCCAGCAGCAAGTATTTGATTATCGTC
TTCAATAGGTAATCGAATCA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MKSYVLTVSCKSTRGIVAAISSYLAEKGCNIIDSSQFDDLDTGKFFMRVSFISEEGLSGSEIGADFAAVAAPFSMEYEFH
DSESRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIPVTKANKVQAEAHIMEVAEQT
GTELIVLARYMQILSDEMCQKMSGKIINIHHSFLPSFKGANPYKQAYGRGVKLIGATAHYVTADLDEGPIIEQDTARITH
AQSPDDYVSIGRDVESQVLARAIHAHIHHRTFINGNRTVVFPASPGSYASERMG

Sequences:

>Translated_294_residues
MKSYVLTVSCKSTRGIVAAISSYLAEKGCNIIDSSQFDDLDTGKFFMRVSFISEEGLSGSEIGADFAAVAAPFSMEYEFH
DSESRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIPVTKANKVQAEAHIMEVAEQT
GTELIVLARYMQILSDEMCQKMSGKIINIHHSFLPSFKGANPYKQAYGRGVKLIGATAHYVTADLDEGPIIEQDTARITH
AQSPDDYVSIGRDVESQVLARAIHAHIHHRTFINGNRTVVFPASPGSYASERMG
>Mature_294_residues
MKSYVLTVSCKSTRGIVAAISSYLAEKGCNIIDSSQFDDLDTGKFFMRVSFISEEGLSGSEIGADFAAVAAPFSMEYEFH
DSESRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVSNHFDYQKVVVNHDIPFHHIPVTKANKVQAEAHIMEVAEQT
GTELIVLARYMQILSDEMCQKMSGKIINIHHSFLPSFKGANPYKQAYGRGVKLIGATAHYVTADLDEGPIIEQDTARITH
AQSPDDYVSIGRDVESQVLARAIHAHIHHRTFINGNRTVVFPASPGSYASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=183, Percent_Identity=31.1475409836066, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI209869995, Length=183, Percent_Identity=31.1475409836066, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI209869993, Length=183, Percent_Identity=31.1475409836066, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=37.9432624113475, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1788846, Length=84, Percent_Identity=36.9047619047619, Blast_Score=64, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17567511, Length=158, Percent_Identity=34.8101265822785, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32567; Mature: 32567

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYVLTVSCKSTRGIVAAISSYLAEKGCNIIDSSQFDDLDTGKFFMRVSFISEEGLSGS
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCC
EIGADFAAVAAPFSMEYEFHDSESRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
NHFDYQKVVVNHDIPFHHIPVTKANKVQAEAHIMEVAEQTGTELIVLARYMQILSDEMCQ
CCCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMSGKIINIHHSFLPSFKGANPYKQAYGRGVKLIGATAHYVTADLDEGPIIEQDTARITH
HHCCCEEEEEHHHCCCCCCCCHHHHHHCCCEEEEEEEEEEEEEECCCCCCCCCCCHHEEC
AQSPDDYVSIGRDVESQVLARAIHAHIHHRTFINGNRTVVFPASPGSYASERMG
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHCCC
>Mature Secondary Structure
MKSYVLTVSCKSTRGIVAAISSYLAEKGCNIIDSSQFDDLDTGKFFMRVSFISEEGLSGS
CCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEHHCCCCCCC
EIGADFAAVAAPFSMEYEFHDSESRMKVLLMVSRFGHCLNDLLYRWKIGALPIDIVGVVS
HHCCCHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHH
NHFDYQKVVVNHDIPFHHIPVTKANKVQAEAHIMEVAEQTGTELIVLARYMQILSDEMCQ
CCCCCEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMSGKIINIHHSFLPSFKGANPYKQAYGRGVKLIGATAHYVTADLDEGPIIEQDTARITH
HHCCCEEEEEHHHCCCCCCCCHHHHHHCCCEEEEEEEEEEEEEECCCCCCCCCCCHHEEC
AQSPDDYVSIGRDVESQVLARAIHAHIHHRTFINGNRTVVFPASPGSYASERMG
CCCCCHHHHHCCCHHHHHHHHHHHHHHHHHEEECCCEEEEEECCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]