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The map label for this gene is gsiB [H]

Identifier: 209551772

GI number: 209551772

Start: 4375382

End: 4376908

Strand: Reverse

Name: gsiB [H]

Synonym: Rleg2_4201

Alternate gene names: 209551772

Gene position: 4376908-4375382 (Counterclockwise)

Preceding gene: 209551773

Following gene: 209551771

Centisome position: 96.45

GC content: 62.08

Gene sequence:

>1527_bases
ATGATCAAGGTTTCCTTCGTACCGGCGCGCTTCGCCGGGCAACTTTCCCTCAGGGCGGCGCTTGCTGCGGGACTTATGAT
GGCGGCGATGGCGCCGGCCGAGGCAGCGAAGACCACTTTCACTCTCGGCATGAGCGTCGAGCCGACCGGCCTCGATCCGA
CGATCGCGGCACCGGTGGCGATCGGCCAGGTGACCTGGCAGAACGTGTTCGAGGGACTGGTGACGATCGACCAGGCCGGC
AAGATCCAGCCGCAGCTGGCAAAAAGCTGGGAGATTTCGCCTGACGGCCTGACCTATACGTTCAAGCTGCAGACCGGCGT
CACATTCCATGACGGCGAGGTCTTCGATGCCGCCAGCGCCAAGTTTACGCTCGACCGCGCCCGCGGCGCGGATTCGGTCA
ATCCGCAGAAACGCTTCTTCGCCTCGATCGCTTCGATCGATACGCCCGATGCCGAAACGCTGGTGCTGCATCTTTCGGCG
CCGACCGGCAGCCTGATCTACTGGCTCGGCTGGCCGGCCTCGGTGATGGTCGCGCCCAAGACCGCGGCCGACGACAAGGC
GACGCCGATCGGCACCGGCCCCTTCAAATTTGTCGGCTGGGCGAAGGGCGACAAGGTCGAACTGGAAAAGAATGCCGATT
ACTGGAACAAGGCTGCGGCCGCCAAGCTCGACAAGGTGACCTTCCGCTTTATCGCCGATCCGCAGGCGCAGGCGGCTGCG
CTGAAATCCGGCGATCTCGATGCCCTTCCGGAATTTGCCGCACCGGAGCTGATGAGTTCTTTCGAGGGCGATGCAAGGCT
TGTCACCAGGATCGGCAATACCGAGCTCAAGGTCGTCGCCGGCATGAACAATGCCAGGAAGCCGTTCGACGACAAACGCG
TGCGCCAGGCGCTGATGATGGCGATCGACCGCAAGACGGTGATCGACGGCGCATGGTCCGGCCTCGGCACGCCGATCGGC
AGCCATTACACGCCGAACGATCCCGGCTATCAGGATATGACGGGCGTGCTGCCTTACGATGTCGAGAAGGCGAAGGCGCT
GCTGGCAGAAGCCGGCTATCCCAACGGCTTCACCTTCACGATCAAATCGCCGCAGATGGCCTATGCGCCGCGCAGCGCTG
AGGTAATGCAGGCGATGTTTGCCGAGATCGGCGTGACGATGAATATCGAGCCGACCGAGTTTCCGGCAAAATGGGTCCAG
GACATCATGAAGGACCGCAACTTCGACATGACGATCGTCGCCCATGCCGAGCCGCTCGACATCGACATCTACGGGCGCGA
TCCCTATTATTTCAACTATAAGAACCCGGTGTTTACCGCGCTGATGAAGAAGGTCCAGGAGACCGCCGATCCCGCCACGC
AGAACGCGATCTACGGCGAGACGCAGAAGATCCTCGCCGAGGACGTGCCGGCGCTCTACCTCTTCGTCATGCCGAAACTC
GGCGTCTGGGACGGAAAGCTGAAGGGGTTGTGGGAGAACGAGCCGATGCCGTCCAATGTGCTGTCCGGGGTTTCGTGGGA
GGAGTGA

Upstream 100 bases:

>100_bases
GCTACACGGATCCGTAATATTCGTCATTATGTGTGCGCCGCGTAGCCATGGTCTGATAATGAAGGGCCTTCGCCTCGACG
CGCGGCCAGACAGGGACAGG

Downstream 100 bases:

>100_bases
GGCGGCAGTAGTTTAGCTGAGTGGTTGGTGGCAGCACGGGCTTGGGAGATATCCGGCATGCAATTGGCCGATAAAGCGCG
TATCCTGCTCTCTGCATCAC

Product: family 5 extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MIKVSFVPARFAGQLSLRAALAAGLMMAAMAPAEAAKTTFTLGMSVEPTGLDPTIAAPVAIGQVTWQNVFEGLVTIDQAG
KIQPQLAKSWEISPDGLTYTFKLQTGVTFHDGEVFDAASAKFTLDRARGADSVNPQKRFFASIASIDTPDAETLVLHLSA
PTGSLIYWLGWPASVMVAPKTAADDKATPIGTGPFKFVGWAKGDKVELEKNADYWNKAAAAKLDKVTFRFIADPQAQAAA
LKSGDLDALPEFAAPELMSSFEGDARLVTRIGNTELKVVAGMNNARKPFDDKRVRQALMMAIDRKTVIDGAWSGLGTPIG
SHYTPNDPGYQDMTGVLPYDVEKAKALLAEAGYPNGFTFTIKSPQMAYAPRSAEVMQAMFAEIGVTMNIEPTEFPAKWVQ
DIMKDRNFDMTIVAHAEPLDIDIYGRDPYYFNYKNPVFTALMKKVQETADPATQNAIYGETQKILAEDVPALYLFVMPKL
GVWDGKLKGLWENEPMPSNVLSGVSWEE

Sequences:

>Translated_508_residues
MIKVSFVPARFAGQLSLRAALAAGLMMAAMAPAEAAKTTFTLGMSVEPTGLDPTIAAPVAIGQVTWQNVFEGLVTIDQAG
KIQPQLAKSWEISPDGLTYTFKLQTGVTFHDGEVFDAASAKFTLDRARGADSVNPQKRFFASIASIDTPDAETLVLHLSA
PTGSLIYWLGWPASVMVAPKTAADDKATPIGTGPFKFVGWAKGDKVELEKNADYWNKAAAAKLDKVTFRFIADPQAQAAA
LKSGDLDALPEFAAPELMSSFEGDARLVTRIGNTELKVVAGMNNARKPFDDKRVRQALMMAIDRKTVIDGAWSGLGTPIG
SHYTPNDPGYQDMTGVLPYDVEKAKALLAEAGYPNGFTFTIKSPQMAYAPRSAEVMQAMFAEIGVTMNIEPTEFPAKWVQ
DIMKDRNFDMTIVAHAEPLDIDIYGRDPYYFNYKNPVFTALMKKVQETADPATQNAIYGETQKILAEDVPALYLFVMPKL
GVWDGKLKGLWENEPMPSNVLSGVSWEE
>Mature_508_residues
MIKVSFVPARFAGQLSLRAALAAGLMMAAMAPAEAAKTTFTLGMSVEPTGLDPTIAAPVAIGQVTWQNVFEGLVTIDQAG
KIQPQLAKSWEISPDGLTYTFKLQTGVTFHDGEVFDAASAKFTLDRARGADSVNPQKRFFASIASIDTPDAETLVLHLSA
PTGSLIYWLGWPASVMVAPKTAADDKATPIGTGPFKFVGWAKGDKVELEKNADYWNKAAAAKLDKVTFRFIADPQAQAAA
LKSGDLDALPEFAAPELMSSFEGDARLVTRIGNTELKVVAGMNNARKPFDDKRVRQALMMAIDRKTVIDGAWSGLGTPIG
SHYTPNDPGYQDMTGVLPYDVEKAKALLAEAGYPNGFTFTIKSPQMAYAPRSAEVMQAMFAEIGVTMNIEPTEFPAKWVQ
DIMKDRNFDMTIVAHAEPLDIDIYGRDPYYFNYKNPVFTALMKKVQETADPATQNAIYGETQKILAEDVPALYLFVMPKL
GVWDGKLKGLWENEPMPSNVLSGVSWEE

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=496, Percent_Identity=29.2338709677419, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789966, Length=514, Percent_Identity=25.875486381323, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1789887, Length=399, Percent_Identity=28.8220551378446, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789397, Length=505, Percent_Identity=23.5643564356436, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=539, Percent_Identity=24.6753246753247, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1787762, Length=521, Percent_Identity=23.6084452975048, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787551, Length=517, Percent_Identity=23.9845261121857, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081878, Length=526, Percent_Identity=24.5247148288973, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 55275; Mature: 55275

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKVSFVPARFAGQLSLRAALAAGLMMAAMAPAEAAKTTFTLGMSVEPTGLDPTIAAPVA
CEEEEEECCHHHCHHHHHHHHHHHHHHHHHCCHHHCCEEEEEECEECCCCCCCCCCCCHH
IGQVTWQNVFEGLVTIDQAGKIQPQLAKSWEISPDGLTYTFKLQTGVTFHDGEVFDAASA
HHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCCCCCEEEEEEEECCCEEECCCEEECCCC
KFTLDRARGADSVNPQKRFFASIASIDTPDAETLVLHLSAPTGSLIYWLGWPASVMVAPK
EEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCCCEEEEECC
TAADDKATPIGTGPFKFVGWAKGDKVELEKNADYWNKAAAAKLDKVTFRFIADPQAQAAA
CCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHEEEEEECCCCHHHHH
LKSGDLDALPEFAAPELMSSFEGDARLVTRIGNTELKVVAGMNNARKPFDDKRVRQALMM
HCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHH
AIDRKTVIDGAWSGLGTPIGSHYTPNDPGYQDMTGVLPYDVEKAKALLAEAGYPNGFTFT
HHCCHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
IKSPQMAYAPRSAEVMQAMFAEIGVTMNIEPTEFPAKWVQDIMKDRNFDMTIVAHAEPLD
ECCCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCE
IDIYGRDPYYFNYKNPVFTALMKKVQETADPATQNAIYGETQKILAEDVPALYLFVMPKL
EEEEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCC
GVWDGKLKGLWENEPMPSNVLSGVSWEE
CCCCCCCCCCCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MIKVSFVPARFAGQLSLRAALAAGLMMAAMAPAEAAKTTFTLGMSVEPTGLDPTIAAPVA
CEEEEEECCHHHCHHHHHHHHHHHHHHHHHCCHHHCCEEEEEECEECCCCCCCCCCCCHH
IGQVTWQNVFEGLVTIDQAGKIQPQLAKSWEISPDGLTYTFKLQTGVTFHDGEVFDAASA
HHHHHHHHHHHHHEEECCCCCCCHHHHHCCCCCCCCEEEEEEEECCCEEECCCEEECCCC
KFTLDRARGADSVNPQKRFFASIASIDTPDAETLVLHLSAPTGSLIYWLGWPASVMVAPK
EEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCCEEEEECCCCEEEEECC
TAADDKATPIGTGPFKFVGWAKGDKVELEKNADYWNKAAAAKLDKVTFRFIADPQAQAAA
CCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCHHHHHHHHHHHHEEEEEECCCCHHHHH
LKSGDLDALPEFAAPELMSSFEGDARLVTRIGNTELKVVAGMNNARKPFDDKRVRQALMM
HCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHH
AIDRKTVIDGAWSGLGTPIGSHYTPNDPGYQDMTGVLPYDVEKAKALLAEAGYPNGFTFT
HHCCHHHCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEE
IKSPQMAYAPRSAEVMQAMFAEIGVTMNIEPTEFPAKWVQDIMKDRNFDMTIVAHAEPLD
ECCCCEEECCCHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCEEEEEECCCCE
IDIYGRDPYYFNYKNPVFTALMKKVQETADPATQNAIYGETQKILAEDVPALYLFVMPKL
EEEEECCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEECCCC
GVWDGKLKGLWENEPMPSNVLSGVSWEE
CCCCCCCCCCCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA