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The map label for this gene is pbpG [H]

Identifier: 209551668

GI number: 209551668

Start: 4271573

End: 4273903

Strand: Reverse

Name: pbpG [H]

Synonym: Rleg2_4097

Alternate gene names: 209551668

Gene position: 4273903-4271573 (Counterclockwise)

Preceding gene: 209551669

Following gene: 209551667

Centisome position: 94.18

GC content: 64.44

Gene sequence:

>2331_bases
ATGGCAGGCAGAGGCAGATCAGGCGACAGAATCGAACCGTCCTTCAGCGGCCGCCCGGCCCGCGACGATGACGAGTTTTC
CCTCGATGCCGATGACCGCATTGCCGGAAGCCGATCCACTCGGCACGGCGGCTCAAAACCGCCGCCGCAGCGTGCCGCCC
CCCGGCGGCGGCGCGAGCCGCGGGAACGCGAAGGCGGCGGCTTGTTCGGCTTCGTGCGCCGCGCGATCTACTGGTGCGTC
GTGCTGTTCATATGGGCCGGCATCGGCGTTGCCGGGCTGGTGGTCTATTATGGCTCACGCATGCCGAGCGCCAGCACATG
GGCGATCCCGGAACGGCCGCCGAACGTCAAGATCACCGCCGTCGACGGCAGCGTCATCGCCAATCGCGGCGCCACCGGCG
GCGAGGCGTTGTCGCTCGAAAACATGTCGCCCTATATTCCGGAAGCCGTTATCGCCATCGAGGACCGGCGCTTCTATTCG
CATTTCGGTGTCGATCCGCTCGGCCTCGGCCGGGCGATCGTGACCAACTTCACGGCTGGCCATATGGTGCAAGGCGGCTC
GACGCTGACGCAGCAGCTCGCCAAGAACCTCTTCCTCTCCCCGGAAAGAACGCTGGAACGCAAGGTGCAGGAGGTGCTGC
TCTCGCTCTGGCTGGAGCAGAAATACACCAAGGATCAGATCCTTGCGATGTATCTCAACCGGGTGTTCTTCGGATCGAAC
GCCTATGGCGTTGAGGCGGCGTCACGCCGCTATTTCAACAAATCGGCGCGTGATGTGAACCTCGGCGAGGCCGCGGTGCT
GGCCGGCCTGCTCAAGGCGCCGTCGCGGCTTTCGCCGGCCCGCGACGCGGACGCCGCCAATGCGCGCGCCCAGCTCGTGC
TTGCGGCAATGCGTGAGCAGGGCTTCATCACCGATTCCGAGGTCAAGACCGCGATGTCGCAGACCCCGGCTTCGGCCAAG
AGCTATTGGTCGGGCGCCGGACACTATGTCGCCGACATGGTGATGGACGAGCTGCCGGGCCTGATCGGCGACGTCAAGGA
AGACGTCATCGTCGATACGACCATCGACAAGTCGCTGGAAAAGAAAGCCGAGCAATCGCTCGTCGACGTGCTCGACAAGG
AGGGCGGAAAGCTCGACGCTTCGCAGGCGGCCCTCGTCTCGATCGACGGCACCGGTGCGATCCGGGCGCTCGTCGGCGGC
AGGGATTATGCGACCAGCCAGTTCAACCGGGCCGTCAAGGCCAAACGCCAGCCAGGCTCCTCGTTCAAGCCCTTCGTCTA
TGCCGCCGCGCTCGAAAAAGGACTGACGCCCTTTTCGGTGTTCAACGACGCGCCGATCCGCATCGGCGACTGGACGCCCG
AAAATTACGAGAAGAAATACAATGGCGAGGTGACGCTGGCGACCGCACTTGCCAAGTCGCTGAACACCGTGGCCGCCCAA
CTGGTGATGTATGACGGGCCGGACCAGGTGATCAAGCTTGCCCACCGGCTCGGCATCGAAAGCGAGCTGCAGCCGAATGC
CTCGATCGCGCTCGGCACCTCTGAAGTGTCGCTGATGGAACTCACCGCCTCCTATGCCGCCTTCATGAACGGCGGCTATA
AGGCGACGCCGCACGTCATCCGCCGGGTGACGACTGCCGAGGGCAAAGTTCTCTACGAAAATACCTATGACAGCCCGCCG
CGTGTGCTGTCCGAGCAGATCGTCGCCGAAATGGATGCGATGATGATGGGCGTCATCGACAACGGCACCGGCAAGAGCGC
CAAGATCCCCGGCTGGCAGGCGGCGGGAAAGACCGGCACGACGCAGAATTCACGCGACGCGCTCTTCGTCGGCTTCACCA
GCAACCTCACGACAGGTGTCTGGTTCGGCAATGACGACGGCAAACCGATGAAAAAGGTGACTGGCGGCGGCCTTCCGGCC
AAAGCCTGGAAAGAATTCATGATCGCCGCCCACAAGGGCCTTTCGCCGGCGCCGCTATTCGGCAACGGCCAGTTGATCGC
CGATCCGAACAATGGTCAGCCGATGGCGCAGGCAGCACCCGGCGATGGACAATCAGCCAGCGGGCAACCGATGACAGCCG
AGGCGCCGCCTTCGACGATCGGCGGCATTATTTCCGGCGTCTTCGGCGGTGACGACAGCGCCAACCGCTATCCGCAGGCG
CCTGTGCGGCAACAGGGCGCGACCTCCGGCAACGGGCCGGTTCCTCCCGCCGACGTTGCCGAAGGCGGCTACGACAGCAT
GGTGCCGCCCGGCGATGTCGGGGGGCCGCAATCGACCTCTTCGGTCCAACCGCGGCGCACCACCCTGCTCGATCTGATCA
TGGGCCAATGA

Upstream 100 bases:

>100_bases
AATCGCCGCATTTACAGTTCAGTCGGGTGACGGCGCCTGCAAGAGTGCCGTTGTGCGAAGTCACGCTTCGCGATCGAACA
TGGGGTGTGACCGGTCGGCG

Downstream 100 bases:

>100_bases
GATCTTTGGCTGCATCGCCGACACAAGCCGGACGCGCCGGAAATCGGCCGCGTCCCGGACGGCGGGCGGCCTCTTTCCCG
ATGACTGTAAAAATTGAATG

Product: penicillin-binding protein, 1A family

Products: NA

Alternate protein names: PBP-2D; PBP2d [H]

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MAGRGRSGDRIEPSFSGRPARDDDEFSLDADDRIAGSRSTRHGGSKPPPQRAAPRRRREPREREGGGLFGFVRRAIYWCV
VLFIWAGIGVAGLVVYYGSRMPSASTWAIPERPPNVKITAVDGSVIANRGATGGEALSLENMSPYIPEAVIAIEDRRFYS
HFGVDPLGLGRAIVTNFTAGHMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLSLWLEQKYTKDQILAMYLNRVFFGSN
AYGVEAASRRYFNKSARDVNLGEAAVLAGLLKAPSRLSPARDADAANARAQLVLAAMREQGFITDSEVKTAMSQTPASAK
SYWSGAGHYVADMVMDELPGLIGDVKEDVIVDTTIDKSLEKKAEQSLVDVLDKEGGKLDASQAALVSIDGTGAIRALVGG
RDYATSQFNRAVKAKRQPGSSFKPFVYAAALEKGLTPFSVFNDAPIRIGDWTPENYEKKYNGEVTLATALAKSLNTVAAQ
LVMYDGPDQVIKLAHRLGIESELQPNASIALGTSEVSLMELTASYAAFMNGGYKATPHVIRRVTTAEGKVLYENTYDSPP
RVLSEQIVAEMDAMMMGVIDNGTGKSAKIPGWQAAGKTGTTQNSRDALFVGFTSNLTTGVWFGNDDGKPMKKVTGGGLPA
KAWKEFMIAAHKGLSPAPLFGNGQLIADPNNGQPMAQAAPGDGQSASGQPMTAEAPPSTIGGIISGVFGGDDSANRYPQA
PVRQQGATSGNGPVPPADVAEGGYDSMVPPGDVGGPQSTSSVQPRRTTLLDLIMGQ

Sequences:

>Translated_776_residues
MAGRGRSGDRIEPSFSGRPARDDDEFSLDADDRIAGSRSTRHGGSKPPPQRAAPRRRREPREREGGGLFGFVRRAIYWCV
VLFIWAGIGVAGLVVYYGSRMPSASTWAIPERPPNVKITAVDGSVIANRGATGGEALSLENMSPYIPEAVIAIEDRRFYS
HFGVDPLGLGRAIVTNFTAGHMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLSLWLEQKYTKDQILAMYLNRVFFGSN
AYGVEAASRRYFNKSARDVNLGEAAVLAGLLKAPSRLSPARDADAANARAQLVLAAMREQGFITDSEVKTAMSQTPASAK
SYWSGAGHYVADMVMDELPGLIGDVKEDVIVDTTIDKSLEKKAEQSLVDVLDKEGGKLDASQAALVSIDGTGAIRALVGG
RDYATSQFNRAVKAKRQPGSSFKPFVYAAALEKGLTPFSVFNDAPIRIGDWTPENYEKKYNGEVTLATALAKSLNTVAAQ
LVMYDGPDQVIKLAHRLGIESELQPNASIALGTSEVSLMELTASYAAFMNGGYKATPHVIRRVTTAEGKVLYENTYDSPP
RVLSEQIVAEMDAMMMGVIDNGTGKSAKIPGWQAAGKTGTTQNSRDALFVGFTSNLTTGVWFGNDDGKPMKKVTGGGLPA
KAWKEFMIAAHKGLSPAPLFGNGQLIADPNNGQPMAQAAPGDGQSASGQPMTAEAPPSTIGGIISGVFGGDDSANRYPQA
PVRQQGATSGNGPVPPADVAEGGYDSMVPPGDVGGPQSTSSVQPRRTTLLDLIMGQ
>Mature_775_residues
AGRGRSGDRIEPSFSGRPARDDDEFSLDADDRIAGSRSTRHGGSKPPPQRAAPRRRREPREREGGGLFGFVRRAIYWCVV
LFIWAGIGVAGLVVYYGSRMPSASTWAIPERPPNVKITAVDGSVIANRGATGGEALSLENMSPYIPEAVIAIEDRRFYSH
FGVDPLGLGRAIVTNFTAGHMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLSLWLEQKYTKDQILAMYLNRVFFGSNA
YGVEAASRRYFNKSARDVNLGEAAVLAGLLKAPSRLSPARDADAANARAQLVLAAMREQGFITDSEVKTAMSQTPASAKS
YWSGAGHYVADMVMDELPGLIGDVKEDVIVDTTIDKSLEKKAEQSLVDVLDKEGGKLDASQAALVSIDGTGAIRALVGGR
DYATSQFNRAVKAKRQPGSSFKPFVYAAALEKGLTPFSVFNDAPIRIGDWTPENYEKKYNGEVTLATALAKSLNTVAAQL
VMYDGPDQVIKLAHRLGIESELQPNASIALGTSEVSLMELTASYAAFMNGGYKATPHVIRRVTTAEGKVLYENTYDSPPR
VLSEQIVAEMDAMMMGVIDNGTGKSAKIPGWQAAGKTGTTQNSRDALFVGFTSNLTTGVWFGNDDGKPMKKVTGGGLPAK
AWKEFMIAAHKGLSPAPLFGNGQLIADPNNGQPMAQAAPGDGQSASGQPMTAEAPPSTIGGIISGVFGGDDSANRYPQAP
VRQQGATSGNGPVPPADVAEGGYDSMVPPGDVGGPQSTSSVQPRRTTLLDLIMGQ

Specific function: Involved in the polymerization and cross-linking of spore peptidoglycan. May be required for synthesis of the spore germ cell wall, the first layer of peptidoglycan synthesized on the surface of the inner forespore membrane [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=508, Percent_Identity=34.0551181102362, Blast_Score=233, Evalue=4e-62,
Organism=Escherichia coli, GI1788867, Length=567, Percent_Identity=30.5114638447972, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87082258, Length=311, Percent_Identity=36.9774919614148, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1789601, Length=235, Percent_Identity=33.1914893617021, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 82643; Mature: 82512

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS01159 WW_DOMAIN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRGRSGDRIEPSFSGRPARDDDEFSLDADDRIAGSRSTRHGGSKPPPQRAAPRRRREP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
REREGGGLFGFVRRAIYWCVVLFIWAGIGVAGLVVYYGSRMPSASTWAIPERPPNVKITA
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
VDGSVIANRGATGGEALSLENMSPYIPEAVIAIEDRRFYSHFGVDPLGLGRAIVTNFTAG
ECCCEEECCCCCCCCEEEECCCCCCCCCEEEEEECCHHHHHCCCCHHHCCHHHHHCCCCC
HMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLSLWLEQKYTKDQILAMYLNRVFFGSN
CEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
AYGVEAASRRYFNKSARDVNLGEAAVLAGLLKAPSRLSPARDADAANARAQLVLAAMREQ
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GFITDSEVKTAMSQTPASAKSYWSGAGHYVADMVMDELPGLIGDVKEDVIVDTTIDKSLE
CCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCEEEECHHHHHHH
KKAEQSLVDVLDKEGGKLDASQAALVSIDGTGAIRALVGGRDYATSQFNRAVKAKRQPGS
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCC
SFKPFVYAAALEKGLTPFSVFNDAPIRIGDWTPENYEKKYNGEVTLATALAKSLNTVAAQ
CCCCHHHHHHHHHCCCCCHHCCCCCEEECCCCCCCHHHHCCCCEEHHHHHHHHHHHHHHH
LVMYDGPDQVIKLAHRLGIESELQPNASIALGTSEVSLMELTASYAAFMNGGYKATPHVI
HHEECCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHH
RRVTTAEGKVLYENTYDSPPRVLSEQIVAEMDAMMMGVIDNGTGKSAKIPGWQAAGKTGT
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCC
TQNSRDALFVGFTSNLTTGVWFGNDDGKPMKKVTGGGLPAKAWKEFMIAAHKGLSPAPLF
CCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
GNGQLIADPNNGQPMAQAAPGDGQSASGQPMTAEAPPSTIGGIISGVFGGDDSANRYPQA
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
PVRQQGATSGNGPVPPADVAEGGYDSMVPPGDVGGPQSTSSVQPRRTTLLDLIMGQ
CHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AGRGRSGDRIEPSFSGRPARDDDEFSLDADDRIAGSRSTRHGGSKPPPQRAAPRRRREP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCC
REREGGGLFGFVRRAIYWCVVLFIWAGIGVAGLVVYYGSRMPSASTWAIPERPPNVKITA
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEE
VDGSVIANRGATGGEALSLENMSPYIPEAVIAIEDRRFYSHFGVDPLGLGRAIVTNFTAG
ECCCEEECCCCCCCCEEEECCCCCCCCCEEEEEECCHHHHHCCCCHHHCCHHHHHCCCCC
HMVQGGSTLTQQLAKNLFLSPERTLERKVQEVLLSLWLEQKYTKDQILAMYLNRVFFGSN
CEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
AYGVEAASRRYFNKSARDVNLGEAAVLAGLLKAPSRLSPARDADAANARAQLVLAAMREQ
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHC
GFITDSEVKTAMSQTPASAKSYWSGAGHYVADMVMDELPGLIGDVKEDVIVDTTIDKSLE
CCCCHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHCHHHHHCCCCEEEECHHHHHHH
KKAEQSLVDVLDKEGGKLDASQAALVSIDGTGAIRALVGGRDYATSQFNRAVKAKRQPGS
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCC
SFKPFVYAAALEKGLTPFSVFNDAPIRIGDWTPENYEKKYNGEVTLATALAKSLNTVAAQ
CCCCHHHHHHHHHCCCCCHHCCCCCEEECCCCCCCHHHHCCCCEEHHHHHHHHHHHHHHH
LVMYDGPDQVIKLAHRLGIESELQPNASIALGTSEVSLMELTASYAAFMNGGYKATPHVI
HHEECCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHH
RRVTTAEGKVLYENTYDSPPRVLSEQIVAEMDAMMMGVIDNGTGKSAKIPGWQAAGKTGT
HHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCC
TQNSRDALFVGFTSNLTTGVWFGNDDGKPMKKVTGGGLPAKAWKEFMIAAHKGLSPAPLF
CCCCCCEEEEEECCCCCEEEEECCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCC
GNGQLIADPNNGQPMAQAAPGDGQSASGQPMTAEAPPSTIGGIISGVFGGDDSANRYPQA
CCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
PVRQQGATSGNGPVPPADVAEGGYDSMVPPGDVGGPQSTSSVQPRRTTLLDLIMGQ
CHHHCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]