Click here to switch to the map view.

The map label for this gene is fabI2 [H]

Identifier: 209551629

GI number: 209551629

Start: 4233202

End: 4234008

Strand: Direct

Name: fabI2 [H]

Synonym: Rleg2_4058

Alternate gene names: 209551629

Gene position: 4233202-4234008 (Clockwise)

Preceding gene: 209551628

Following gene: 209551630

Centisome position: 93.28

GC content: 62.08

Gene sequence:

>807_bases
ATGACTGGAATCATGCAGGGTAAGCGCGGCCTCATCATGGGCGTCGCAAACAACCACTCGATCGCCTGGGGAATTTCAAA
AGCTCTTGCCGCACAGGGTGCGGAACTTGCCTTCACCTATCAGGGCGATGCGCTGGGCAAGCGCGTCAAGCCGCTTGCCG
CCGAAGTCGGCTCGGATTTCGTGATGCCCTGCGACGTCGAGGACATCGCCTCGGTCGATGCCCTGGTCGACGCGATCGAG
GAGCGCTGGGGCAAGCTCGATTTCATCGTCCATGCCATCGGTTTTTCCGACAAGAACGAGCTGAAGGGTCTCTACGCCGA
TACGACGCGCGAGAATTTCAGCCGCACCATGGTCATTTCCTGTTTCTCCTTCACCGAGATCGCCAAGCGCTGCGCGCCGT
TGATGGAAGATGGCGGTGCGATGCTGACGCTAACCTATAACGGCTCGACGCGGGTCATCCCGAATTACAATGTCATGGGT
GTCGCCAAGGCAGCGCTCGAGGCTTCGGTGCGTTATCTCGCTGCCGATTACGGCCCGCGCGGCATCCGCGTCAACGCCAT
ATCGGCCGGCCCGATCCGCACGCTCGCCGGCGCCGGCATCTCCGATGCGCGCGCGATCCTCTCCTGGAACCAGCGCAATG
CGCCCCTGCGCAAGACCGTCACCATCGACCAGGTCGGCAACTCCGCGCTTTACCTGCTTTCCGACCTTTCCGCCGGCGTT
ACCGGCGAAATCCACTTCGTCGACGCGGGCTTCAACGTTACCTCCATGCCGACGCTGGACACGCTGCGCAGGGCGGACGT
CGAGTAA

Upstream 100 bases:

>100_bases
CGTATCGACGATGCGAAGATCGATATCGCCCTCTCCAACTCCTTCGGCTTCGGCGGGACGAACGCCACGCTCGTCTTCCA
GCGCTATAACGGATAACAAA

Downstream 100 bases:

>100_bases
GAACTTGTTCAGATGTTCTAAAAGGCCATGCGCATCATGATGCGCATGGCCTTGCTGTTTTTGGCTGCAAGAATTATATT
GGAAAGGGAGTTGGGGCGAT

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase 2 [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGDALGKRVKPLAAEVGSDFVMPCDVEDIASVDALVDAIE
ERWGKLDFIVHAIGFSDKNELKGLYADTTRENFSRTMVISCFSFTEIAKRCAPLMEDGGAMLTLTYNGSTRVIPNYNVMG
VAKAALEASVRYLAADYGPRGIRVNAISAGPIRTLAGAGISDARAILSWNQRNAPLRKTVTIDQVGNSALYLLSDLSAGV
TGEIHFVDAGFNVTSMPTLDTLRRADVE

Sequences:

>Translated_268_residues
MTGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGDALGKRVKPLAAEVGSDFVMPCDVEDIASVDALVDAIE
ERWGKLDFIVHAIGFSDKNELKGLYADTTRENFSRTMVISCFSFTEIAKRCAPLMEDGGAMLTLTYNGSTRVIPNYNVMG
VAKAALEASVRYLAADYGPRGIRVNAISAGPIRTLAGAGISDARAILSWNQRNAPLRKTVTIDQVGNSALYLLSDLSAGV
TGEIHFVDAGFNVTSMPTLDTLRRADVE
>Mature_267_residues
TGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGDALGKRVKPLAAEVGSDFVMPCDVEDIASVDALVDAIEE
RWGKLDFIVHAIGFSDKNELKGLYADTTRENFSRTMVISCFSFTEIAKRCAPLMEDGGAMLTLTYNGSTRVIPNYNVMGV
AKAALEASVRYLAADYGPRGIRVNAISAGPIRTLAGAGISDARAILSWNQRNAPLRKTVTIDQVGNSALYLLSDLSAGVT
GEIHFVDAGFNVTSMPTLDTLRRADVE

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=52.1235521235521, Blast_Score=270, Evalue=6e-74,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=26.8482490272374, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789378, Length=258, Percent_Identity=27.1317829457364, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI2367365, Length=260, Percent_Identity=28.0769230769231, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787905, Length=240, Percent_Identity=28.3333333333333, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28653; Mature: 28522

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGDALGKRVKPLAAEVGSDF
CCCCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
VMPCDVEDIASVDALVDAIEERWGKLDFIVHAIGFSDKNELKGLYADTTRENFSRTMVIS
EECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHCCCEECCCCHHCCCEEEEEE
CFSFTEIAKRCAPLMEDGGAMLTLTYNGSTRVIPNYNVMGVAKAALEASVRYLAADYGPR
EHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCC
GIRVNAISAGPIRTLAGAGISDARAILSWNQRNAPLRKTVTIDQVGNSALYLLSDLSAGV
CEEEEEECCCCHHHHHCCCCCHHHHHEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCC
TGEIHFVDAGFNVTSMPTLDTLRRADVE
CEEEEEEECCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TGIMQGKRGLIMGVANNHSIAWGISKALAAQGAELAFTYQGDALGKRVKPLAAEVGSDF
CCCCCCCCEEEEEECCCCEEEHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHCCCC
VMPCDVEDIASVDALVDAIEERWGKLDFIVHAIGFSDKNELKGLYADTTRENFSRTMVIS
EECCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHHCCCEECCCCHHCCCEEEEEE
CFSFTEIAKRCAPLMEDGGAMLTLTYNGSTRVIPNYNVMGVAKAALEASVRYLAADYGPR
EHHHHHHHHHHHHHHHCCCEEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCC
GIRVNAISAGPIRTLAGAGISDARAILSWNQRNAPLRKTVTIDQVGNSALYLLSDLSAGV
CEEEEEECCCCHHHHHCCCCCHHHHHEECCCCCCCCEEEEEEECCCCCEEEEEECCCCCC
TGEIHFVDAGFNVTSMPTLDTLRRADVE
CEEEEEEECCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]