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The map label for this gene is purH [H]

Identifier: 209551486

GI number: 209551486

Start: 4061273

End: 4062889

Strand: Reverse

Name: purH [H]

Synonym: Rleg2_3915

Alternate gene names: 209551486

Gene position: 4062889-4061273 (Counterclockwise)

Preceding gene: 209551487

Following gene: 209551483

Centisome position: 89.53

GC content: 63.82

Gene sequence:

>1617_bases
ATGGCCGTTATTTCCAAGAAGATCCCCGCCCCCGACAAGGTCGAAATCAAGACCGCCCTCCTCTCCGTCTTCGACAAGAC
CGGGATCGTCGAACTCGCCCAGGCACTGTCGGAACAGGGCGTGCGGCTGCTGTCGACCGGCGGCACCTACAAGGCGATCG
CCGCCGCCGGCCTTGCCGTTACCGATGTTTCCGAAATTACCGGCTTTCCCGAGATCATGGACGGGCGGGTCAAGACGCTG
CATCCGACGGTGCATGGCGGCCTGCTGGCGATCCGCGACGATAGCGAACACCAGGAGGCGATGAAAACCCACGGCATCGA
GGCCATCGACCTCGCCGTCATCAACCTCTATCCCTTCGAAGACGTGCGCGCCGCCGGCGGCGATTATCCGACGACCGTCG
AAAATATCGACATCGGCGGCCCGGCGATGATCCGCGCTTCGGCCAAGAACCATGCCTATGTGACGATCCTGACCGATCCG
AACGACTATGCCGAATTCACCGAGCAGCTTTCCGCGGATGGTGGCAAGACCGCCTACGCCTTCCGGCAGCGCATGGCCGC
CAAGGCCTATGCCCGCACCGCGGCCTATGACGCTGTGATTTCCAACTGGTTCGCAGAAGCGCTGTCGATCGACACGCCGC
GCCACCGCGTTATCGGCGGTGCGCTGAAGGAAGAGATGCGTTACGGCGAAAATCCGCACCAGAAGGCCGCCTTTTACGTC
ACCGGCGAGAAGCGCCCGGGCGTTTCGACGGCTGCCCTCCTCCAGGGCAAGCAGCTCTCCTACAACAATATCAACGATAC
CGATGCCGCTTACGAGCTGGTCGCCGAGTTCCTGCCGGAAAAGGAGCCGGCCTGCGCCATCATCAAACATGCCAATCCCT
GTGGCGTCGCCACCGGGTCGAGCCTGGTCGAGGCCTATCGGCGGGCGTTGGCCTGCGACAGCGTTTCCGCCTTCGGCGGC
ATCATTGCACTCAATCGGACGCTGGATGCCGAAACGGCTGAGGAGATCGTCAAGCTCTTCACCGAAGTGATCATCGCGCC
CGATGTGACCGAGGAGGCGAAGGCGATCATCGCCCGCAAGCCGAACCTGCGGCTGCTGTCGGCCGGCGGCCTGCCCGATC
CGCGTGCCGCGGGCCTGACGGCGAAGACCGTTTCCGGCGGCCTGCTGGTCCAGAGCCGCGACAACGGCATGGTCGAGGAT
CTGGAGCTCAAGGTCGTCACCAAGCGCGCGCCGACGGCTCAGGAGCTTGATGATATGAAGTTCGCCTTCAAGATCGGCAA
ACACGTGAAATCGAACGCCGTGGTCTATGCCAAGGACGGCCAGACCGCCGGCATCGGCGCCGGCCAGATGAGCCGGGTCG
ATTCTGCCCGTATCGCCGCGCTGAAGGCGGAAGAAGCCGCCAAGGCGCTCGGCCTTGCCGTGCCGATGACGCATGGCTCG
GCAGTCGCCTCCGAAGCCTTCCTGCCGTTTGCCGACGGTCTCTTGTCGATGATCGCAGCGGGGGCGACGGCGGTGATCCA
GCCTGGCGGTTCGATGCGCGACCAGGAAGTCATCGATGCCGCCGACGAACACGGCATTGCGATGGTCTTTACCGGCATGC
GCCATTTCCGGCACTGA

Upstream 100 bases:

>100_bases
CGTCCTGGAGCAGGATGATCTTAGGCCCGAGCCGGCCTGAAATCCGAATCCTGCTCTAACCCAAGCGGATGTCTCCCGAA
GCCCGAACGGAGAAGGTTTG

Downstream 100 bases:

>100_bases
ATGAGGACTGCGCCATGCTTCTGTCATGGTCCGACGCGCGTGACCCTACGCGCGATCGGCCGGATAGGGCGTGCGGACGA
GCAGCACCAGGCCACCGGCG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MAVISKKIPAPDKVEIKTALLSVFDKTGIVELAQALSEQGVRLLSTGGTYKAIAAAGLAVTDVSEITGFPEIMDGRVKTL
HPTVHGGLLAIRDDSEHQEAMKTHGIEAIDLAVINLYPFEDVRAAGGDYPTTVENIDIGGPAMIRASAKNHAYVTILTDP
NDYAEFTEQLSADGGKTAYAFRQRMAAKAYARTAAYDAVISNWFAEALSIDTPRHRVIGGALKEEMRYGENPHQKAAFYV
TGEKRPGVSTAALLQGKQLSYNNINDTDAAYELVAEFLPEKEPACAIIKHANPCGVATGSSLVEAYRRALACDSVSAFGG
IIALNRTLDAETAEEIVKLFTEVIIAPDVTEEAKAIIARKPNLRLLSAGGLPDPRAAGLTAKTVSGGLLVQSRDNGMVED
LELKVVTKRAPTAQELDDMKFAFKIGKHVKSNAVVYAKDGQTAGIGAGQMSRVDSARIAALKAEEAAKALGLAVPMTHGS
AVASEAFLPFADGLLSMIAAGATAVIQPGGSMRDQEVIDAADEHGIAMVFTGMRHFRH

Sequences:

>Translated_538_residues
MAVISKKIPAPDKVEIKTALLSVFDKTGIVELAQALSEQGVRLLSTGGTYKAIAAAGLAVTDVSEITGFPEIMDGRVKTL
HPTVHGGLLAIRDDSEHQEAMKTHGIEAIDLAVINLYPFEDVRAAGGDYPTTVENIDIGGPAMIRASAKNHAYVTILTDP
NDYAEFTEQLSADGGKTAYAFRQRMAAKAYARTAAYDAVISNWFAEALSIDTPRHRVIGGALKEEMRYGENPHQKAAFYV
TGEKRPGVSTAALLQGKQLSYNNINDTDAAYELVAEFLPEKEPACAIIKHANPCGVATGSSLVEAYRRALACDSVSAFGG
IIALNRTLDAETAEEIVKLFTEVIIAPDVTEEAKAIIARKPNLRLLSAGGLPDPRAAGLTAKTVSGGLLVQSRDNGMVED
LELKVVTKRAPTAQELDDMKFAFKIGKHVKSNAVVYAKDGQTAGIGAGQMSRVDSARIAALKAEEAAKALGLAVPMTHGS
AVASEAFLPFADGLLSMIAAGATAVIQPGGSMRDQEVIDAADEHGIAMVFTGMRHFRH
>Mature_537_residues
AVISKKIPAPDKVEIKTALLSVFDKTGIVELAQALSEQGVRLLSTGGTYKAIAAAGLAVTDVSEITGFPEIMDGRVKTLH
PTVHGGLLAIRDDSEHQEAMKTHGIEAIDLAVINLYPFEDVRAAGGDYPTTVENIDIGGPAMIRASAKNHAYVTILTDPN
DYAEFTEQLSADGGKTAYAFRQRMAAKAYARTAAYDAVISNWFAEALSIDTPRHRVIGGALKEEMRYGENPHQKAAFYVT
GEKRPGVSTAALLQGKQLSYNNINDTDAAYELVAEFLPEKEPACAIIKHANPCGVATGSSLVEAYRRALACDSVSAFGGI
IALNRTLDAETAEEIVKLFTEVIIAPDVTEEAKAIIARKPNLRLLSAGGLPDPRAAGLTAKTVSGGLLVQSRDNGMVEDL
ELKVVTKRAPTAQELDDMKFAFKIGKHVKSNAVVYAKDGQTAGIGAGQMSRVDSARIAALKAEEAAKALGLAVPMTHGSA
VASEAFLPFADGLLSMIAAGATAVIQPGGSMRDQEVIDAADEHGIAMVFTGMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=480, Percent_Identity=39.1666666666667, Blast_Score=266, Evalue=5e-71,
Organism=Escherichia coli, GI1790439, Length=536, Percent_Identity=54.8507462686567, Blast_Score=531, Evalue=1e-152,
Organism=Caenorhabditis elegans, GI71985564, Length=606, Percent_Identity=33.3333333333333, Blast_Score=281, Evalue=9e-76,
Organism=Caenorhabditis elegans, GI71985574, Length=306, Percent_Identity=31.0457516339869, Blast_Score=102, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323768, Length=605, Percent_Identity=34.8760330578512, Blast_Score=298, Evalue=1e-81,
Organism=Saccharomyces cerevisiae, GI6323056, Length=601, Percent_Identity=34.4425956738769, Blast_Score=296, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24649832, Length=602, Percent_Identity=34.21926910299, Blast_Score=280, Evalue=1e-75,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57028; Mature: 56896

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVISKKIPAPDKVEIKTALLSVFDKTGIVELAQALSEQGVRLLSTGGTYKAIAAAGLAV
CCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCE
TDVSEITGFPEIMDGRVKTLHPTVHGGLLAIRDDSEHQEAMKTHGIEAIDLAVINLYPFE
ECHHHHCCCHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEECCCH
DVRAAGGDYPTTVENIDIGGPAMIRASAKNHAYVTILTDPNDYAEFTEQLSADGGKTAYA
HHHHCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHH
FRQRMAAKAYARTAAYDAVISNWFAEALSIDTPRHRVIGGALKEEMRYGENPHQKAAFYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
TGEKRPGVSTAALLQGKQLSYNNINDTDAAYELVAEFLPEKEPACAIIKHANPCGVATGS
ECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCH
SLVEAYRRALACDSVSAFGGIIALNRTLDAETAEEIVKLFTEVIIAPDVTEEAKAIIARK
HHHHHHHHHHHHCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PNLRLLSAGGLPDPRAAGLTAKTVSGGLLVQSRDNGMVEDLELKVVTKRAPTAQELDDMK
CCEEEEECCCCCCCCCCCCEEEECCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHH
FAFKIGKHVKSNAVVYAKDGQTAGIGAGQMSRVDSARIAALKAEEAAKALGLAVPMTHGS
HHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCEEEECCCCC
AVASEAFLPFADGLLSMIAAGATAVIQPGGSMRDQEVIDAADEHGIAMVFTGMRHFRH
HHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
AVISKKIPAPDKVEIKTALLSVFDKTGIVELAQALSEQGVRLLSTGGTYKAIAAAGLAV
CCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHCCCCE
TDVSEITGFPEIMDGRVKTLHPTVHGGLLAIRDDSEHQEAMKTHGIEAIDLAVINLYPFE
ECHHHHCCCHHHHCCCCEEECCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEEEEECCCH
DVRAAGGDYPTTVENIDIGGPAMIRASAKNHAYVTILTDPNDYAEFTEQLSADGGKTAYA
HHHHCCCCCCCCCCEECCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHCCCCCHHHH
FRQRMAAKAYARTAAYDAVISNWFAEALSIDTPRHRVIGGALKEEMRYGENPHQKAAFYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEE
TGEKRPGVSTAALLQGKQLSYNNINDTDAAYELVAEFLPEKEPACAIIKHANPCGVATGS
ECCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCH
SLVEAYRRALACDSVSAFGGIIALNRTLDAETAEEIVKLFTEVIIAPDVTEEAKAIIARK
HHHHHHHHHHHHCCHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
PNLRLLSAGGLPDPRAAGLTAKTVSGGLLVQSRDNGMVEDLELKVVTKRAPTAQELDDMK
CCEEEEECCCCCCCCCCCCEEEECCCCEEEEECCCCCEEEEEEEEEECCCCCHHHHHHHH
FAFKIGKHVKSNAVVYAKDGQTAGIGAGQMSRVDSARIAALKAEEAAKALGLAVPMTHGS
HHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCEEEECCCCC
AVASEAFLPFADGLLSMIAAGATAVIQPGGSMRDQEVIDAADEHGIAMVFTGMRHFRH
HHHHHCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA