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The map label for this gene is leuC [H]

Identifier: 209551330

GI number: 209551330

Start: 3882539

End: 3883948

Strand: Direct

Name: leuC [H]

Synonym: Rleg2_3759

Alternate gene names: 209551330

Gene position: 3882539-3883948 (Clockwise)

Preceding gene: 209551329

Following gene: 209551331

Centisome position: 85.56

GC content: 64.75

Gene sequence:

>1410_bases
ATGAGCGCACCGCGTACCCTCTACGACAAGATCTGGGACGATCACCTGGTCGACGAACAGCCGGACGGCACCTGTCTTCT
CTACATCGACCGCCATCTGGTCCACGAAGTCACCTCGCCGCAGGCGTTCGAAGGCCTGCGGATGACCGGCCGCAAGGTTC
GCGCCCCGGAAAAGACGCTTGCCGTCGTCGACCATAACGTTCCGACCTCGCCCGACCGCCATCTCGGCATCAAGAACGAG
GAAAGCCGCATCCAGGTGGAAGCGCTGGCGACCAACGCCGCCGAATTCGGCGTCGAATATTATTCGGCAAGCGACAAGCG
CCAGGGCATCGTTCACATCGTCGGCCCGGAACAGGGTTTCACCCTGCCCGGCATGACCATCGTCTGCGGTGACAGCCACA
CCTCGACGCACGGCGCCTTCGGCGCGCTGGCGCACGGCATCGGCACCTCCGAGGTCGAGCATGTGCTGGCGACCCAGACG
CTGATCCAGAAGAAGGCGAAGAACATGCTGGTGCGAGTCGACGGCCTGCTGCCGCCGCACGTCACCGCCAAGGACATCAT
CCTCGCCATCATCGGCGAAATCGGCACCGCCGGCGGCACCGGCCACGTCATCGAATTTGCCGGCGAAGCGATCCGCTCGC
TGTCGATGGAAGGCCGCATGACCATCTGCAACATGACGATCGAAGGCGGCGCCCGCGCCGGCCTGATCGCCCCTGACGAA
AAGACCTTCGAATACATCAAGGGCAAGCCGCGCGCGCCGAAGGGCGAAGCACTGGAACAGGCGATCGCCTACTGGAAGAC
GCTGCAGACCGACGAGGGCGCGCATTACGACCGCGTCGTCGTGCTCGATGCCGCCAGCCTGCCACCGATCGTCTCCTGGG
GCTCCTCGCCCGAGGACGTCATCTCCGTCCAGGGCATCGTGCCGAACCCCGACGACATCCAGGACGAGACCAAGCGCACC
TCCAAATGGCGCGCCCTCGACTATATGGGTCTTAAGCCCGGCACCAAGATGACCGACATCACGCTTGACCGCGTCTTCAT
CGGCTCCTGCACCAACGGCCGCATCGAGGACCTGCGCGAAGTCGCCAAGGTCGTCGAAGGCAAGACGGTTGCTTCAACCG
TCGATGCGATGATCGTTCCGGGCTCCGGCCTCGTCAAGGAACAGGCGGAAGCCGAAGGCCTCGACAAGATCTTCAAGGCC
GCCGGTTTCGACTGGCGCGAGCCGGGCTGCTCCATGTGCCTTGCGATGAACGACGACCGCCTGAAGCCGGGCGAGCGCTG
CGCTTCGACCTCGAACCGCAACTTCGAGGGTCGCCAGGGCTTCAAGGGCCGCACGCACCTCGTCTCGCCGGCAATGGCGG
CGGCCGCAGCGATCGCCGGTCACTTCGTCGACATCCGCGAGTGGAACTGA

Upstream 100 bases:

>100_bases
TGCGCTCAACAGCCGACTTCGTTGATCGAATGACGGCCGGCGGAGCTTGATCCGCTTTTTACTCGATTTCCCAAAAGCAT
CCAAAAACGGACTTAACGCC

Downstream 100 bases:

>100_bases
TCCGTCGTCCGACGGAAGAGAATGAAAACCGCCCGATGCGCTCTGCGCATCGGGCGGTTTTGCTTTTCATGGCGGCGGTG
CTTCCCCATCCCGCTGTCAT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSAPRTLYDKIWDDHLVDEQPDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNE
ESRIQVEALATNAAEFGVEYYSASDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGLLPPHVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDE
KTFEYIKGKPRAPKGEALEQAIAYWKTLQTDEGAHYDRVVVLDAASLPPIVSWGSSPEDVISVQGIVPNPDDIQDETKRT
SKWRALDYMGLKPGTKMTDITLDRVFIGSCTNGRIEDLREVAKVVEGKTVASTVDAMIVPGSGLVKEQAEAEGLDKIFKA
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIREWN

Sequences:

>Translated_469_residues
MSAPRTLYDKIWDDHLVDEQPDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNE
ESRIQVEALATNAAEFGVEYYSASDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQT
LIQKKAKNMLVRVDGLLPPHVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDE
KTFEYIKGKPRAPKGEALEQAIAYWKTLQTDEGAHYDRVVVLDAASLPPIVSWGSSPEDVISVQGIVPNPDDIQDETKRT
SKWRALDYMGLKPGTKMTDITLDRVFIGSCTNGRIEDLREVAKVVEGKTVASTVDAMIVPGSGLVKEQAEAEGLDKIFKA
AGFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIREWN
>Mature_468_residues
SAPRTLYDKIWDDHLVDEQPDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTLAVVDHNVPTSPDRHLGIKNEE
SRIQVEALATNAAEFGVEYYSASDKRQGIVHIVGPEQGFTLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTL
IQKKAKNMLVRVDGLLPPHVTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDEK
TFEYIKGKPRAPKGEALEQAIAYWKTLQTDEGAHYDRVVVLDAASLPPIVSWGSSPEDVISVQGIVPNPDDIQDETKRTS
KWRALDYMGLKPGTKMTDITLDRVFIGSCTNGRIEDLREVAKVVEGKTVASTVDAMIVPGSGLVKEQAEAEGLDKIFKAA
GFDWREPGCSMCLAMNDDRLKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIREWN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=476, Percent_Identity=26.2605042016807, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI8659555, Length=384, Percent_Identity=25.78125, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI41352693, Length=401, Percent_Identity=25.9351620947631, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786259, Length=462, Percent_Identity=66.017316017316, Blast_Score=618, Evalue=1e-178,
Organism=Escherichia coli, GI87081781, Length=357, Percent_Identity=24.0896358543417, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=25.9052924791086, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25149337, Length=480, Percent_Identity=27.5, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=382, Percent_Identity=29.0575916230367, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=29, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=405, Percent_Identity=27.6543209876543, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=64.755838641189, Blast_Score=618, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6320440, Length=428, Percent_Identity=27.1028037383178, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=29.1338582677165, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=493, Percent_Identity=25.9634888438134, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365315, Length=478, Percent_Identity=26.1506276150628, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17864292, Length=478, Percent_Identity=26.1506276150628, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI28571643, Length=480, Percent_Identity=26.0416666666667, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI161076999, Length=382, Percent_Identity=27.4869109947644, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24645686, Length=378, Percent_Identity=28.3068783068783, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=27.7628032345013, Blast_Score=92, Evalue=8e-19,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50828; Mature: 50697

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPRTLYDKIWDDHLVDEQPDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTL
CCCCHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEE
AVVDHNVPTSPDRHLGIKNEESRIQVEALATNAAEFGVEYYSASDKRQGIVHIVGPEQGF
EEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCHHHCCCCCCCCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGLLPPH
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCH
KTFEYIKGKPRAPKGEALEQAIAYWKTLQTDEGAHYDRVVVLDAASLPPIVSWGSSPEDV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCHHHE
ISVQGIVPNPDDIQDETKRTSKWRALDYMGLKPGTKMTDITLDRVFIGSCTNGRIEDLRE
EEEECCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
VAKVVEGKTVASTVDAMIVPGSGLVKEQAEAEGLDKIFKAAGFDWREPGCSMCLAMNDDR
HHHHHCCCHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIREWN
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEEECCC
>Mature Secondary Structure 
SAPRTLYDKIWDDHLVDEQPDGTCLLYIDRHLVHEVTSPQAFEGLRMTGRKVRAPEKTL
CCCHHHHHHHHCCCCCCCCCCCEEEEEECHHHHHHCCCCHHHHHHHHCCCCCCCCCCEE
AVVDHNVPTSPDRHLGIKNEESRIQVEALATNAAEFGVEYYSASDKRQGIVHIVGPEQGF
EEEECCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHCHHHCCCCCCCCCEEEEECCCCCC
TLPGMTIVCGDSHTSTHGAFGALAHGIGTSEVEHVLATQTLIQKKAKNMLVRVDGLLPPH
CCCCEEEEECCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCEEEEECCCCCCC
VTAKDIILAIIGEIGTAGGTGHVIEFAGEAIRSLSMEGRMTICNMTIEGGARAGLIAPDE
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCH
KTFEYIKGKPRAPKGEALEQAIAYWKTLQTDEGAHYDRVVVLDAASLPPIVSWGSSPEDV
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEECCCCCHHHE
ISVQGIVPNPDDIQDETKRTSKWRALDYMGLKPGTKMTDITLDRVFIGSCTNGRIEDLRE
EEEECCCCCCCCCHHHHHHHHHHHEEEECCCCCCCCEEEEEEEEEEEECCCCCHHHHHHH
VAKVVEGKTVASTVDAMIVPGSGLVKEQAEAEGLDKIFKAAGFDWREPGCSMCLAMNDDR
HHHHHCCCHHHHHHCEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCC
LKPGERCASTSNRNFEGRQGFKGRTHLVSPAMAAAAAIAGHFVDIREWN
CCCHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA