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The map label for this gene is yxeN [H]

Identifier: 209551328

GI number: 209551328

Start: 3881452

End: 3882264

Strand: Direct

Name: yxeN [H]

Synonym: Rleg2_3757

Alternate gene names: 209551328

Gene position: 3881452-3882264 (Clockwise)

Preceding gene: 209551327

Following gene: 209551329

Centisome position: 85.53

GC content: 61.13

Gene sequence:

>813_bases
ATGGCCTTGCGACCATCTCCCGACAGACACGTCAAGGACGACTATCCCTGGTGGCTGGTCGCGCTCGTCGCGATCGGCAT
CGTCCTTGCCGCCGTCATCGTCACCAACGATATCTACGCCCAGGTTTTCCGCACCGTCGTCAACGGAGTCGGCATCACAG
TCTTCGTCACGCTGGTCGCCTTCGTGCTCGCCACCGTGCTCGGTCTCGGCATCTGCCTGCTCGGCATGGCCGACAGCCAG
ATCGCGCGGCAGATCGCCCGTTTCTACATCGAGATCATCCGCGGCATCCCGATCCTTGTCCTCTTGTTCTACGTTGCCTT
CGTCGGCGCCCCGGCCCTGGTCGCCGGCTATAATTTTCTGATTTCGCCGCTGGTGAGTTCAGGCATGGCCGAGCCCATCC
TGGTGCGCGATCTCTCGCTGATGTGGCGGGCCATCATTGCGCTGACGATCGGTTACTCCTCCTTCATCGCTGAAATCTTC
CGGGCCGGTTTCCAATCGGTCGATCTCGGCCAGATCGAGGCGGCCAAGGCGCTCGGCCTTTCCCGCTACCGGCGCTTCCG
CTCGGTGGTTTTCCCGCAGGCGATCCGCGTGATCTTCCCGCCGCTTTCCAACGATTTCGTTTCGATGGTGAAGGATTCAT
CGCTCGTCTCAGTACTCGGCGTCGCCGACATCACCCAGATGGGCAAGGTCTATGCCGCCGGCTCCTTCCGCTTCTTCGAG
ACCTATTCGATCGTCACCTATATCTACCTGATCCTGACGATCGGCCTGTCGCTGGCGCTGCGGCGGACCGAGAAATGGAT
GAAGTCGCGGTAA

Upstream 100 bases:

>100_bases
GGCGCCATCTCGGCGCTCAAGGCCGACGGCACGCTGGACGCGCTGAACAAGAAGTGGTTCCTCGACTACAAGATGGGCGA
ATGACGCCGAGCCATTGCTG

Downstream 100 bases:

>100_bases
AGGGTGGCACGCAGAGCAGGCCGCGGACGGGCTGTTGATGATTCAAATTGAAGCCGCCGCGAAAAATCGTTATATGCAGG
CAATGGAATCCAAGTTCAGA

Product: polar amino acid ABC transporter inner membrane subunit

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MALRPSPDRHVKDDYPWWLVALVAIGIVLAAVIVTNDIYAQVFRTVVNGVGITVFVTLVAFVLATVLGLGICLLGMADSQ
IARQIARFYIEIIRGIPILVLLFYVAFVGAPALVAGYNFLISPLVSSGMAEPILVRDLSLMWRAIIALTIGYSSFIAEIF
RAGFQSVDLGQIEAAKALGLSRYRRFRSVVFPQAIRVIFPPLSNDFVSMVKDSSLVSVLGVADITQMGKVYAAGSFRFFE
TYSIVTYIYLILTIGLSLALRRTEKWMKSR

Sequences:

>Translated_270_residues
MALRPSPDRHVKDDYPWWLVALVAIGIVLAAVIVTNDIYAQVFRTVVNGVGITVFVTLVAFVLATVLGLGICLLGMADSQ
IARQIARFYIEIIRGIPILVLLFYVAFVGAPALVAGYNFLISPLVSSGMAEPILVRDLSLMWRAIIALTIGYSSFIAEIF
RAGFQSVDLGQIEAAKALGLSRYRRFRSVVFPQAIRVIFPPLSNDFVSMVKDSSLVSVLGVADITQMGKVYAAGSFRFFE
TYSIVTYIYLILTIGLSLALRRTEKWMKSR
>Mature_269_residues
ALRPSPDRHVKDDYPWWLVALVAIGIVLAAVIVTNDIYAQVFRTVVNGVGITVFVTLVAFVLATVLGLGICLLGMADSQI
ARQIARFYIEIIRGIPILVLLFYVAFVGAPALVAGYNFLISPLVSSGMAEPILVRDLSLMWRAIIALTIGYSSFIAEIFR
AGFQSVDLGQIEAAKALGLSRYRRFRSVVFPQAIRVIFPPLSNDFVSMVKDSSLVSVLGVADITQMGKVYAAGSFRFFET
YSIVTYIYLILTIGLSLALRRTEKWMKSR

Specific function: Probably part of the ABC transporter complex yxeMNO that could be involved in amino-acid import. May transport S- methylcysteine. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=227, Percent_Identity=35.6828193832599, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788226, Length=212, Percent_Identity=29.7169811320755, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI48994930, Length=220, Percent_Identity=33.6363636363636, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1788645, Length=224, Percent_Identity=27.2321428571429, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788646, Length=208, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1786874, Length=221, Percent_Identity=24.4343891402715, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI87082239, Length=221, Percent_Identity=27.6018099547511, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787087, Length=241, Percent_Identity=25.7261410788382, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=26.3392857142857, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29769; Mature: 29638

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALRPSPDRHVKDDYPWWLVALVAIGIVLAAVIVTNDIYAQVFRTVVNGVGITVFVTLVA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
FVLATVLGLGICLLGMADSQIARQIARFYIEIIRGIPILVLLFYVAFVGAPALVAGYNFL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
ISPLVSSGMAEPILVRDLSLMWRAIIALTIGYSSFIAEIFRAGFQSVDLGQIEAAKALGL
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
SRYRRFRSVVFPQAIRVIFPPLSNDFVSMVKDSSLVSVLGVADITQMGKVYAAGSFRFFE
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TYSIVTYIYLILTIGLSLALRRTEKWMKSR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]