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The map label for this gene is dgoD [H]

Identifier: 209551237

GI number: 209551237

Start: 3793423

End: 3794586

Strand: Reverse

Name: dgoD [H]

Synonym: Rleg2_3665

Alternate gene names: 209551237

Gene position: 3794586-3793423 (Counterclockwise)

Preceding gene: 209551238

Following gene: 209551236

Centisome position: 83.62

GC content: 61.68

Gene sequence:

>1164_bases
ATGACGACGAAGCTTAAAATTACGGCGATCAAGCCCTATCCGGTATGGGTCGGAACGCGCAACCAGATGCTGGTCAAGGT
CGAGACCGACAGCGGCATCTTCGGCTGGGGCGAGAGCGGCTTGAGCGGTCGCGAGAAGGCCGTGGCCGGCGCCGTCGAGC
ACTATCGCGAGTTTCTCATCGGCCGCGACCCGATGCAGATCGGTCGGATTTGGCAAGAGGCTTATCGCAGTCAATACTTC
GAAGGCGGGCGTGTCCTGCAGGCAGCGATTTCAGCCATCGACATTGCTCTTCACGACATCAAGGGCAAAGCGCTTGGGGT
GCCTGCCTACGAACTGCTGGGCGGCAAGCAGCGCGACCGCATTCCGACCTTCGCCTCGACCGGGGACGAGGCCGAGGGCG
AGGTTGCCATCGAACGCGCCCGCGAACTGCGCGCACAAGGCTGGCAGGCGATCCGTTTCTTCCCCATCGGGCAAAACAAC
AGGGACATTTTTGAGCCGCGGGAGTCGATCGGCGCGACCGCAGCGATGCTGAACAAGGCGCGCCAGGCGCTGGGCGACGA
CGTTGTCCTCGGCATCGATTATCATCATCGGCTGTCGGTGGCAGAGGCGGCGAGCTTCTGTAACAAGCTCGGCCGTGGCG
TGCTTGATTTCCTCGAGGAGCCGATACGAGACGAGGCGCCCGAGGCCTACGAATCCCTGCGCACGATGACTGACATCCCG
TTCGCCATCGGCGAGGAATTTGCCAGCAAGTGGCAATTCCTGCCCTACATCGAGCGTGGCATCCATCAGTTCAACCGGCT
CGATGTTTGCAATGTTGGCGGGCTCACCGAAGCGATGAAGGTCGCCGGTTGGAGTGAGGCGCATTATGTGGACCTGATGC
CGCACAATCCGCTCGGCCCAGTCTGCACCGCCGCGACCATCCATCTCGCCGCCGCGGTACCGAATTTCGCCTGGCTCGAG
ACTAGAGCACCCGAAGCAAAGCTCGGCTTTGACAATTCCGATTTCTTTCCCGTTCAACCACGGCTCGACGGGGCGGACTA
TCCGGTCAGCGATCTGCCGGGTCTCGGCGTCGAGGTCAACGAAGAGGCGATCAAGGCGGAGAGCTTTCGGTTCTGGGAAG
CGCCGCATCTGAAGCGCCGCGACGGTTCTGTCACAAACTGGTAG

Upstream 100 bases:

>100_bases
AGTTCGATCAGGAGCGAATGCATCTGTTCGACGGCGAAACAGAAATGGCCCTCAAGGCCAACTGAGACCCAGCGGATGCG
TGTGACAAACGAGAACGTAG

Downstream 100 bases:

>100_bases
TTTCATCCACCGGAGCCTAAAATGACCCATACGCCCGACATTCCGCGACCGTCCAAAGAACTGATCGACGCACTGAAGGA
AATCGGCGCCGCGACGGTTG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MTTKLKITAIKPYPVWVGTRNQMLVKVETDSGIFGWGESGLSGREKAVAGAVEHYREFLIGRDPMQIGRIWQEAYRSQYF
EGGRVLQAAISAIDIALHDIKGKALGVPAYELLGGKQRDRIPTFASTGDEAEGEVAIERARELRAQGWQAIRFFPIGQNN
RDIFEPRESIGATAAMLNKARQALGDDVVLGIDYHHRLSVAEAASFCNKLGRGVLDFLEEPIRDEAPEAYESLRTMTDIP
FAIGEEFASKWQFLPYIERGIHQFNRLDVCNVGGLTEAMKVAGWSEAHYVDLMPHNPLGPVCTAATIHLAAAVPNFAWLE
TRAPEAKLGFDNSDFFPVQPRLDGADYPVSDLPGLGVEVNEEAIKAESFRFWEAPHLKRRDGSVTNW

Sequences:

>Translated_387_residues
MTTKLKITAIKPYPVWVGTRNQMLVKVETDSGIFGWGESGLSGREKAVAGAVEHYREFLIGRDPMQIGRIWQEAYRSQYF
EGGRVLQAAISAIDIALHDIKGKALGVPAYELLGGKQRDRIPTFASTGDEAEGEVAIERARELRAQGWQAIRFFPIGQNN
RDIFEPRESIGATAAMLNKARQALGDDVVLGIDYHHRLSVAEAASFCNKLGRGVLDFLEEPIRDEAPEAYESLRTMTDIP
FAIGEEFASKWQFLPYIERGIHQFNRLDVCNVGGLTEAMKVAGWSEAHYVDLMPHNPLGPVCTAATIHLAAAVPNFAWLE
TRAPEAKLGFDNSDFFPVQPRLDGADYPVSDLPGLGVEVNEEAIKAESFRFWEAPHLKRRDGSVTNW
>Mature_386_residues
TTKLKITAIKPYPVWVGTRNQMLVKVETDSGIFGWGESGLSGREKAVAGAVEHYREFLIGRDPMQIGRIWQEAYRSQYFE
GGRVLQAAISAIDIALHDIKGKALGVPAYELLGGKQRDRIPTFASTGDEAEGEVAIERARELRAQGWQAIRFFPIGQNNR
DIFEPRESIGATAAMLNKARQALGDDVVLGIDYHHRLSVAEAASFCNKLGRGVLDFLEEPIRDEAPEAYESLRTMTDIPF
AIGEEFASKWQFLPYIERGIHQFNRLDVCNVGGLTEAMKVAGWSEAHYVDLMPHNPLGPVCTAATIHLAAAVPNFAWLET
RAPEAKLGFDNSDFFPVQPRLDGADYPVSDLPGLGVEVNEEAIKAESFRFWEAPHLKRRDGSVTNW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=391, Percent_Identity=33.2480818414322, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1787864, Length=395, Percent_Identity=30.8860759493671, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI226510960, Length=363, Percent_Identity=24.7933884297521, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 43000; Mature: 42869

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKLKITAIKPYPVWVGTRNQMLVKVETDSGIFGWGESGLSGREKAVAGAVEHYREFLI
CCCEEEEEEECCCEEEEECCCCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHC
GRDPMQIGRIWQEAYRSQYFEGGRVLQAAISAIDIALHDIKGKALGVPAYELLGGKQRDR
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCCCCCC
IPTFASTGDEAEGEVAIERARELRAQGWQAIRFFPIGQNNRDIFEPRESIGATAAMLNKA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
RQALGDDVVLGIDYHHRLSVAEAASFCNKLGRGVLDFLEEPIRDEAPEAYESLRTMTDIP
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
FAIGEEFASKWQFLPYIERGIHQFNRLDVCNVGGLTEAMKVAGWSEAHYVDLMPHNPLGP
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCH
VCTAATIHLAAAVPNFAWLETRAPEAKLGFDNSDFFPVQPRLDGADYPVSDLPGLGVEVN
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCCCCHHCCCCCCCEEC
EEAIKAESFRFWEAPHLKRRDGSVTNW
HHHHHHHCEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTKLKITAIKPYPVWVGTRNQMLVKVETDSGIFGWGESGLSGREKAVAGAVEHYREFLI
CCEEEEEEECCCEEEEECCCCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHHHC
GRDPMQIGRIWQEAYRSQYFEGGRVLQAAISAIDIALHDIKGKALGVPAYELLGGKQRDR
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCCCCCCC
IPTFASTGDEAEGEVAIERARELRAQGWQAIRFFPIGQNNRDIFEPRESIGATAAMLNKA
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
RQALGDDVVLGIDYHHRLSVAEAASFCNKLGRGVLDFLEEPIRDEAPEAYESLRTMTDIP
HHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
FAIGEEFASKWQFLPYIERGIHQFNRLDVCNVGGLTEAMKVAGWSEAHYVDLMPHNPLGP
HHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCH
VCTAATIHLAAAVPNFAWLETRAPEAKLGFDNSDFFPVQPRLDGADYPVSDLPGLGVEVN
HHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCEECCCCCCCCCCCHHCCCCCCCEEC
EEAIKAESFRFWEAPHLKRRDGSVTNW
HHHHHHHCEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]