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The map label for this gene is hipO [H]

Identifier: 209551183

GI number: 209551183

Start: 3732915

End: 3734078

Strand: Direct

Name: hipO [H]

Synonym: Rleg2_3609

Alternate gene names: 209551183

Gene position: 3732915-3734078 (Clockwise)

Preceding gene: 209551178

Following gene: 209551184

Centisome position: 82.26

GC content: 65.98

Gene sequence:

>1164_bases
ATGCCGATTTTGAACAGAGCCGCCGAATTGCAGGACGAGGTCGCCGAATGGCGCCGTCATATCCACGCCCGGCCCGAGCT
GCTCTTCGCGGTTGAGAACACGGCCGCCTTCGTCGCTGAAAAGCTCAAGGAATTCGGCGTCGACGAGATCGTCACCGGCA
TCGGCCGCACCGGCGTCGTCGGCCTGATCAAGGGCAAGGGCGAAGGCCGCCGCACCGTCGGCCTGCGCGCCGACATGGAC
GCCCTGCCGCTGACCGAAATATCAGGCAAGGCCTGGGCCTCGAAGACGCCGGGCAAGATGCATGCCTGCGGCCATGACGG
CCACACCGCCATGCTGCTCGGCGCTGCGAAATACCTGGCCGAAACCCGTAACTTCAGCGGCAATATCGCCGTCATCTTCC
AGCCCGCCGAAGAAGGCGGCGGCGGCGGCAACCTGATGGTCAAGGACGGCATGATGGAGCGCTTCGGCATTGAAGAGGTC
TACGGCATGCACAATCTGCCGGGCCTGCCTGTGGGACAGTTCGCCACCCGCAAGGGCGCGATCATGGCGGCGACCGACGA
ATTCACCGTCACCATCAAGGGCCGCGGCGGCCACGCCGCCCAGCCGCACCGGACGATCGACCCGATCGCCATCAGCGCCC
AGATCGTCGCCAACCTGCAGATGATCGCCTCGCGCACCGCCGATCCGATCAGCTCGGTCGTCGTCTCGGTGACGAAGTTC
AATGCCGGCTTCGCCCATAACGTCATTCCGAACGACGCAACCTTCGCCGGCACGGTGCGCACTCTCGACCCCGAGGTGCG
CACGCTGGCCGAAACGCGGTTCCGGCAGATCGTCGAGGGTGTCGCCGCCGCCCACGGCGCCGAGGCCGAAATCAGCTTCC
ACCGCAACTACCCCGTCACCGTCAATCATCCGGACGAGACCGAACATGCGGTGGCCACCGCCAGCGCCATTGCCGGCGAG
GCCAACGTCAACGCCGAGATCGACCCGATGATGGGCGGCGAGGATTTCTCCTACATGCTGAACGCCCGCCCCGGCGCCTT
CATCTTCATCGGCAACGGCGACAGCGCCGGCCTGCACAACCCGGCCTACGACTTCAACGACGAAGCCATCGCCCACGGCA
TCTCCTACTGGGTCCGCCTGGCCGAACAACGCCTCGGCCTCTGA

Upstream 100 bases:

>100_bases
TAATTTCCACAGGCTCACGCCTCAGCCGCCTCACACCCCCCGCCTCACGCCCTTGTGATGCGATGAACGATTGCCGGGAT
TTCATAACAGGAACGCCAAC

Downstream 100 bases:

>100_bases
CAACAATTAAGGCTTGGCTTCGCGCCAAGCCTTTTGTATGCATGGCATGCAAGTGGTCCCGTAGCTCAGCAGGATAGAGC
ACCAGATTCCTAATCTGGGG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MPILNRAAELQDEVAEWRRHIHARPELLFAVENTAAFVAEKLKEFGVDEIVTGIGRTGVVGLIKGKGEGRRTVGLRADMD
ALPLTEISGKAWASKTPGKMHACGHDGHTAMLLGAAKYLAETRNFSGNIAVIFQPAEEGGGGGNLMVKDGMMERFGIEEV
YGMHNLPGLPVGQFATRKGAIMAATDEFTVTIKGRGGHAAQPHRTIDPIAISAQIVANLQMIASRTADPISSVVVSVTKF
NAGFAHNVIPNDATFAGTVRTLDPEVRTLAETRFRQIVEGVAAAHGAEAEISFHRNYPVTVNHPDETEHAVATASAIAGE
ANVNAEIDPMMGGEDFSYMLNARPGAFIFIGNGDSAGLHNPAYDFNDEAIAHGISYWVRLAEQRLGL

Sequences:

>Translated_387_residues
MPILNRAAELQDEVAEWRRHIHARPELLFAVENTAAFVAEKLKEFGVDEIVTGIGRTGVVGLIKGKGEGRRTVGLRADMD
ALPLTEISGKAWASKTPGKMHACGHDGHTAMLLGAAKYLAETRNFSGNIAVIFQPAEEGGGGGNLMVKDGMMERFGIEEV
YGMHNLPGLPVGQFATRKGAIMAATDEFTVTIKGRGGHAAQPHRTIDPIAISAQIVANLQMIASRTADPISSVVVSVTKF
NAGFAHNVIPNDATFAGTVRTLDPEVRTLAETRFRQIVEGVAAAHGAEAEISFHRNYPVTVNHPDETEHAVATASAIAGE
ANVNAEIDPMMGGEDFSYMLNARPGAFIFIGNGDSAGLHNPAYDFNDEAIAHGISYWVRLAEQRLGL
>Mature_386_residues
PILNRAAELQDEVAEWRRHIHARPELLFAVENTAAFVAEKLKEFGVDEIVTGIGRTGVVGLIKGKGEGRRTVGLRADMDA
LPLTEISGKAWASKTPGKMHACGHDGHTAMLLGAAKYLAETRNFSGNIAVIFQPAEEGGGGGNLMVKDGMMERFGIEEVY
GMHNLPGLPVGQFATRKGAIMAATDEFTVTIKGRGGHAAQPHRTIDPIAISAQIVANLQMIASRTADPISSVVVSVTKFN
AGFAHNVIPNDATFAGTVRTLDPEVRTLAETRFRQIVEGVAAAHGAEAEISFHRNYPVTVNHPDETEHAVATASAIAGEA
NVNAEIDPMMGGEDFSYMLNARPGAFIFIGNGDSAGLHNPAYDFNDEAIAHGISYWVRLAEQRLGL

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=433, Percent_Identity=27.0207852193995, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41341; Mature: 41210

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPILNRAAELQDEVAEWRRHIHARPELLFAVENTAAFVAEKLKEFGVDEIVTGIGRTGVV
CCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCHHHHHHHCCCCCEE
GLIKGKGEGRRTVGLRADMDALPLTEISGKAWASKTPGKMHACGHDGHTAMLLGAAKYLA
EEEECCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHH
ETRNFSGNIAVIFQPAEEGGGGGNLMVKDGMMERFGIEEVYGMHNLPGLPVGQFATRKGA
HHCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHCHHHHHCCCCCCCCCCCHHHCCCCE
IMAATDEFTVTIKGRGGHAAQPHRTIDPIAISAQIVANLQMIASRTADPISSVVVSVTKF
EEEECCEEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
NAGFAHNVIPNDATFAGTVRTLDPEVRTLAETRFRQIVEGVAAAHGAEAEISFHRNYPVT
CCCCCCCCCCCCCEEECEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEE
VNHPDETEHAVATASAIAGEANVNAEIDPMMGGEDFSYMLNARPGAFIFIGNGDSAGLHN
ECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCC
PAYDFNDEAIAHGISYWVRLAEQRLGL
CCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PILNRAAELQDEVAEWRRHIHARPELLFAVENTAAFVAEKLKEFGVDEIVTGIGRTGVV
CCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHCCHHHHHHHCCCCCEE
GLIKGKGEGRRTVGLRADMDALPLTEISGKAWASKTPGKMHACGHDGHTAMLLGAAKYLA
EEEECCCCCCEEEEEECCCCCCCCHHCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHH
ETRNFSGNIAVIFQPAEEGGGGGNLMVKDGMMERFGIEEVYGMHNLPGLPVGQFATRKGA
HHCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHCHHHHHCCCCCCCCCCCHHHCCCCE
IMAATDEFTVTIKGRGGHAAQPHRTIDPIAISAQIVANLQMIASRTADPISSVVVSVTKF
EEEECCEEEEEEECCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
NAGFAHNVIPNDATFAGTVRTLDPEVRTLAETRFRQIVEGVAAAHGAEAEISFHRNYPVT
CCCCCCCCCCCCCEEECEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEE
VNHPDETEHAVATASAIAGEANVNAEIDPMMGGEDFSYMLNARPGAFIFIGNGDSAGLHN
ECCCCCHHHHHHHHHHHCCCCCCCCEECCCCCCCCCEEEEECCCCEEEEEECCCCCCCCC
PAYDFNDEAIAHGISYWVRLAEQRLGL
CCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]