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The map label for this gene is gloB [H]

Identifier: 209551158

GI number: 209551158

Start: 3709245

End: 3710015

Strand: Direct

Name: gloB [H]

Synonym: Rleg2_3582

Alternate gene names: 209551158

Gene position: 3709245-3710015 (Clockwise)

Preceding gene: 209551153

Following gene: 209551159

Centisome position: 81.74

GC content: 60.57

Gene sequence:

>771_bases
ATGAAACCTTTGGAATTGGACGTTTTTCTCTGCCGCACCGACAATTTCGGCGTTCTCGTCCACGACCCGGAAACAGGATT
TACCGCGGCGATCGACGCGCCGGAGGAGGCGCCGATCCTGGAAGCGGCGACACGCCGCGGCTGGAAGATCACCCATATCT
TCACCACCCATCACCACACCGACCATGTCGCCGCCAATCTGGCGCTGAAGGAGCAGTTCGGCTGCGAGATCATCGGCCCG
ATCAACGAGGCCATTGCCATTCCCGGCCTCGACCGGACGATGGCCGATGGAGACAGCTTCCTGTTCGGCGATCACACGAT
CAATGTCATCGAGACACCTGGCCACACTGCCGGCCACATCTGCTATCACTTCGTCGACGACAAGCTGCTCTTTGCCGCCG
ACACGCTGTTTGCCCTCGGCTGCGGCCGCCTGTTCGAACGCCCCGCCGCCGACATGTGGCATTCGCTGCAGAAGCTTGCC
GTGCTGCCCGATGAGACCGCCATCTATTTCGGTCACGAATATACGCTCTCCAACGCCCGTTTCGCACTGACGGTCGATCC
CGGCAATGAACGGCTGAAGGGCCGTGCCGCCGAGATCGAGGCCTTGCGCGCCGACGGCAAATTCACCATCCCGACGACGC
TGGGGCTGGAAAAGGAAACTAACCCGTTCCTGCGCGCCGCCGATCCGGCAATCCGCCGCAATCTGCTGATGGAAGGCAAG
ACCAATGAAGAAGTCTTTGCCGAGATCCGCAAGCGCAAGGATAATTTCTGA

Upstream 100 bases:

>100_bases
TCGGCGTGCATTGCGACAAATGTTATCAGCGGGCGGTTGGACTTCAAGGCCGGACCCTCTACATTTCCGCGAGACTCGCA
TGACAAGGGACTATTGCACG

Downstream 100 bases:

>100_bases
TGTCGCCTGAGGACATCATCCGCGAACTCGGCATGCAGCCGCATCCGGAAGGCGGCTGGTATGTGCAGACATTCCGCGAC
ACGGCAGGCGGAGACCGCGG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKPLELDVFLCRTDNFGVLVHDPETGFTAAIDAPEEAPILEAATRRGWKITHIFTTHHHTDHVAANLALKEQFGCEIIGP
INEAIAIPGLDRTMADGDSFLFGDHTINVIETPGHTAGHICYHFVDDKLLFAADTLFALGCGRLFERPAADMWHSLQKLA
VLPDETAIYFGHEYTLSNARFALTVDPGNERLKGRAAEIEALRADGKFTIPTTLGLEKETNPFLRAADPAIRRNLLMEGK
TNEEVFAEIRKRKDNF

Sequences:

>Translated_256_residues
MKPLELDVFLCRTDNFGVLVHDPETGFTAAIDAPEEAPILEAATRRGWKITHIFTTHHHTDHVAANLALKEQFGCEIIGP
INEAIAIPGLDRTMADGDSFLFGDHTINVIETPGHTAGHICYHFVDDKLLFAADTLFALGCGRLFERPAADMWHSLQKLA
VLPDETAIYFGHEYTLSNARFALTVDPGNERLKGRAAEIEALRADGKFTIPTTLGLEKETNPFLRAADPAIRRNLLMEGK
TNEEVFAEIRKRKDNF
>Mature_256_residues
MKPLELDVFLCRTDNFGVLVHDPETGFTAAIDAPEEAPILEAATRRGWKITHIFTTHHHTDHVAANLALKEQFGCEIIGP
INEAIAIPGLDRTMADGDSFLFGDHTINVIETPGHTAGHICYHFVDDKLLFAADTLFALGCGRLFERPAADMWHSLQKLA
VLPDETAIYFGHEYTLSNARFALTVDPGNERLKGRAAEIEALRADGKFTIPTTLGLEKETNPFLRAADPAIRRNLLMEGK
TNEEVFAEIRKRKDNF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=262, Percent_Identity=33.587786259542, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI94538322, Length=262, Percent_Identity=33.587786259542, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI14150041, Length=236, Percent_Identity=35.5932203389831, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI116642887, Length=219, Percent_Identity=32.8767123287671, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI21703352, Length=255, Percent_Identity=31.3725490196078, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI46361987, Length=194, Percent_Identity=36.0824742268041, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI41327741, Length=188, Percent_Identity=30.8510638297872, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786406, Length=248, Percent_Identity=38.7096774193548, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787158, Length=141, Percent_Identity=31.9148936170213, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=259, Percent_Identity=36.2934362934363, Blast_Score=147, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6324614, Length=288, Percent_Identity=30.2083333333333, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320478, Length=236, Percent_Identity=30.0847457627119, Blast_Score=90, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356335, Length=265, Percent_Identity=33.2075471698113, Blast_Score=150, Evalue=6e-37,
Organism=Drosophila melanogaster, GI24667711, Length=265, Percent_Identity=33.2075471698113, Blast_Score=150, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24667703, Length=265, Percent_Identity=33.2075471698113, Blast_Score=150, Evalue=9e-37,
Organism=Drosophila melanogaster, GI221330176, Length=178, Percent_Identity=30.8988764044944, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28463; Mature: 28463

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLELDVFLCRTDNFGVLVHDPETGFTAAIDAPEEAPILEAATRRGWKITHIFTTHHHT
CCCEEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCHHHHHHCCCEEEEEEEEECCCC
DHVAANLALKEQFGCEIIGPINEAIAIPGLDRTMADGDSFLFGDHTINVIETPGHTAGHI
CHHHEEEEEHHHCCCEEECCCCCEEECCCCCCHHCCCCCEEECCEEEEEEECCCCCCCCE
CYHFVDDKLLFAADTLFALGCGRLFERPAADMWHSLQKLAVLPDETAIYFGHEYTLSNAR
EEEEECCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCE
FALTVDPGNERLKGRAAEIEALRADGKFTIPTTLGLEKETNPFLRAADPAIRRNLLMEGK
EEEEECCCCHHHCCCHHHEEEEECCCCEECCEEECCCCCCCCCEECCCHHHHHHEEECCC
TNEEVFAEIRKRKDNF
CCHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPLELDVFLCRTDNFGVLVHDPETGFTAAIDAPEEAPILEAATRRGWKITHIFTTHHHT
CCCEEEEEEEEECCCCEEEEECCCCCEEEEECCCCCCCHHHHHHCCCEEEEEEEEECCCC
DHVAANLALKEQFGCEIIGPINEAIAIPGLDRTMADGDSFLFGDHTINVIETPGHTAGHI
CHHHEEEEEHHHCCCEEECCCCCEEECCCCCCHHCCCCCEEECCEEEEEEECCCCCCCCE
CYHFVDDKLLFAADTLFALGCGRLFERPAADMWHSLQKLAVLPDETAIYFGHEYTLSNAR
EEEEECCEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCE
FALTVDPGNERLKGRAAEIEALRADGKFTIPTTLGLEKETNPFLRAADPAIRRNLLMEGK
EEEEECCCCHHHCCCHHHEEEEECCCCEECCEEECCCCCCCCCEECCCHHHHHHEEECCC
TNEEVFAEIRKRKDNF
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA