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The map label for this gene is bepF [H]

Identifier: 209551089

GI number: 209551089

Start: 3631640

End: 3632836

Strand: Direct

Name: bepF [H]

Synonym: Rleg2_3513

Alternate gene names: 209551089

Gene position: 3631640-3632836 (Clockwise)

Preceding gene: 209551088

Following gene: 209551090

Centisome position: 80.03

GC content: 61.24

Gene sequence:

>1197_bases
ATGCGTGTGAGATTGGATCAGGGTCTGCACCTCTTGATGGTGCTCGCCGGCATGGCAATTCTTGCCGGCTGCGAGGAAAG
CGGCAACACCTATGTTCCTCCGCCGCCACCTCCGGTACGGGTCGCACAGCCGGTCCAGCAGCCGGTGACGCTCTATTTCG
AACTCACCGGCAATACGGCGCCGCTCAATGCCGTCGATATCGAGGCCCGCGTCCAGGGCTATATCCAGTCCATCGACTAC
CAGGACGGCATGATGGTGATGAAGGGCGCCAAGCTTTTCGGCATCGAGCGCGACACCTATCAGGCGCAACTGGATCAGGC
AAAGGCATCGCTCGCCTCGCAGCAGGCCTCCCAGGTCGGCGCAAAGCAGGAATATGACCGGCAGCTCAATCTCATGAAGC
AACAGGTCACCACCCAGACCGCGGTCGACAGCGCCAAGGCGACCCTCGACGAGGCGAATGCTTCCATCCTCAACGCCCAG
GCCAATGTCGAACTCGCGACGATCAACCTCGGCTATACCGAAGTGCTTGCTCCCTTCGACGGCATCGTCACGGATCACCT
TGTCGATATCGGCACGCTGGTCGGCGTGTCCGGCCCCACCAAACTCGCCAGCATCGTCCAGACCGATCCGCTGTATGCCT
ATTTCAACGTCAGCGAGACGCAGGTGCTGATGATCAAGGAAACCATGGCAAAGCAGGGACATACCTTCAGGCAGACGGAC
CTTCCGAGCATACCGGCGGAGATCGGTTTACAGACGGAAGAGGGTTACCCGCACAAGGGACATCTCGATTACGTATCGCC
TCAGCTCGACGCCTCCACAGGCACGCTTCAGGTCCGCGCCCTGTTCGACAACAAGGACCACGCCATGCTGCCCGGCCTCT
TCGTGCGGGTTCGGGTGCCGGTCGGCCACAACGACAAGGCTCTGCTGGTGCGCGACGACACCATCGGAACCAACCAACTC
GGCAGCTACGTACTCGTTCTCGGCAAGGACGACGTGGTCGAGCAGAAACAGGTCAAGACCGGGCAGCGGGAAGGCGCGCT
CCGCGTCATCCAATCCGGCCTCGATCCGGCTGACTGGGTGGTGACCCAAGGGATCCAGCAGGCCATTCCCGGCAGCAAGG
TCACGCCCGAGAAAATCGAAATGAACCCGACGGCGAATGCCGCCGGTGATGCCAAGGCCAACTCCACCACGCAATGA

Upstream 100 bases:

>100_bases
CAAAAGGACGCTGTAGATCTTTGAAATCGCAGCATAATTCATCCAATCGATTCGATTGGATGAATTATGCAGCAGGCTCG
TAAACATCGGGGCGGCGGCA

Downstream 100 bases:

>100_bases
ATTCGCCACCACGCTTCATCTGAACGTCTGAGGACAACAGCATGATCTCGCGTTTCTTCATTGAGCGACCGGTACTCGCC
AACGTCTTGGCATTGGTTTT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRVRLDQGLHLLMVLAGMAILAGCEESGNTYVPPPPPPVRVAQPVQQPVTLYFELTGNTAPLNAVDIEARVQGYIQSIDY
QDGMMVMKGAKLFGIERDTYQAQLDQAKASLASQQASQVGAKQEYDRQLNLMKQQVTTQTAVDSAKATLDEANASILNAQ
ANVELATINLGYTEVLAPFDGIVTDHLVDIGTLVGVSGPTKLASIVQTDPLYAYFNVSETQVLMIKETMAKQGHTFRQTD
LPSIPAEIGLQTEEGYPHKGHLDYVSPQLDASTGTLQVRALFDNKDHAMLPGLFVRVRVPVGHNDKALLVRDDTIGTNQL
GSYVLVLGKDDVVEQKQVKTGQREGALRVIQSGLDPADWVVTQGIQQAIPGSKVTPEKIEMNPTANAAGDAKANSTTQ

Sequences:

>Translated_398_residues
MRVRLDQGLHLLMVLAGMAILAGCEESGNTYVPPPPPPVRVAQPVQQPVTLYFELTGNTAPLNAVDIEARVQGYIQSIDY
QDGMMVMKGAKLFGIERDTYQAQLDQAKASLASQQASQVGAKQEYDRQLNLMKQQVTTQTAVDSAKATLDEANASILNAQ
ANVELATINLGYTEVLAPFDGIVTDHLVDIGTLVGVSGPTKLASIVQTDPLYAYFNVSETQVLMIKETMAKQGHTFRQTD
LPSIPAEIGLQTEEGYPHKGHLDYVSPQLDASTGTLQVRALFDNKDHAMLPGLFVRVRVPVGHNDKALLVRDDTIGTNQL
GSYVLVLGKDDVVEQKQVKTGQREGALRVIQSGLDPADWVVTQGIQQAIPGSKVTPEKIEMNPTANAAGDAKANSTTQ
>Mature_398_residues
MRVRLDQGLHLLMVLAGMAILAGCEESGNTYVPPPPPPVRVAQPVQQPVTLYFELTGNTAPLNAVDIEARVQGYIQSIDY
QDGMMVMKGAKLFGIERDTYQAQLDQAKASLASQQASQVGAKQEYDRQLNLMKQQVTTQTAVDSAKATLDEANASILNAQ
ANVELATINLGYTEVLAPFDGIVTDHLVDIGTLVGVSGPTKLASIVQTDPLYAYFNVSETQVLMIKETMAKQGHTFRQTD
LPSIPAEIGLQTEEGYPHKGHLDYVSPQLDASTGTLQVRALFDNKDHAMLPGLFVRVRVPVGHNDKALLVRDDTIGTNQL
GSYVLVLGKDDVVEQKQVKTGQREGALRVIQSGLDPADWVVTQGIQQAIPGSKVTPEKIEMNPTANAAGDAKANSTTQ

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=364, Percent_Identity=31.043956043956, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1786668, Length=387, Percent_Identity=32.0413436692506, Blast_Score=161, Evalue=5e-41,
Organism=Escherichia coli, GI1789665, Length=363, Percent_Identity=31.129476584022, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI87082036, Length=340, Percent_Identity=28.2352941176471, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42957; Mature: 42957

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRLDQGLHLLMVLAGMAILAGCEESGNTYVPPPPPPVRVAQPVQQPVTLYFELTGNTA
CEEECCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCC
PLNAVDIEARVQGYIQSIDYQDGMMVMKGAKLFGIERDTYQAQLDQAKASLASQQASQVG
CCCEEEHHHHHHHHHHHCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
AKQEYDRQLNLMKQQVTTQTAVDSAKATLDEANASILNAQANVELATINLGYTEVLAPFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCHHHHHCCCC
GIVTDHLVDIGTLVGVSGPTKLASIVQTDPLYAYFNVSETQVLMIKETMAKQGHTFRQTD
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEHHHHHCCCCEECCC
LPSIPAEIGLQTEEGYPHKGHLDYVSPQLDASTGTLQVRALFDNKDHAMLPGLFVRVRVP
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEC
VGHNDKALLVRDDTIGTNQLGSYVLVLGKDDVVEQKQVKTGQREGALRVIQSGLDPADWV
CCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
VTQGIQQAIPGSKVTPEKIEMNPTANAAGDAKANSTTQ
HHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVRLDQGLHLLMVLAGMAILAGCEESGNTYVPPPPPPVRVAQPVQQPVTLYFELTGNTA
CEEECCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCCEEEEEEECCCCC
PLNAVDIEARVQGYIQSIDYQDGMMVMKGAKLFGIERDTYQAQLDQAKASLASQQASQVG
CCCEEEHHHHHHHHHHHCCCCCCEEEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHCC
AKQEYDRQLNLMKQQVTTQTAVDSAKATLDEANASILNAQANVELATINLGYTEVLAPFD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEEECCHHHHHCCCC
GIVTDHLVDIGTLVGVSGPTKLASIVQTDPLYAYFNVSETQVLMIKETMAKQGHTFRQTD
HHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCEEEEEEHHHHHCCCCEECCC
LPSIPAEIGLQTEEGYPHKGHLDYVSPQLDASTGTLQVRALFDNKDHAMLPGLFVRVRVP
CCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEC
VGHNDKALLVRDDTIGTNQLGSYVLVLGKDDVVEQKQVKTGQREGALRVIQSGLDPADWV
CCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCCHHHHHHHCCCCHHHHH
VTQGIQQAIPGSKVTPEKIEMNPTANAAGDAKANSTTQ
HHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]