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The map label for this gene is pfpI [H]

Identifier: 209551084

GI number: 209551084

Start: 3627087

End: 3627683

Strand: Direct

Name: pfpI [H]

Synonym: Rleg2_3508

Alternate gene names: 209551084

Gene position: 3627087-3627683 (Clockwise)

Preceding gene: 209551083

Following gene: 209551085

Centisome position: 79.93

GC content: 62.98

Gene sequence:

>597_bases
ATGCCAGCTTCCAAAATCCTGATGATCACGGGTGATTTCACCGAAGATTACGAAACCATGGTGCCGTTCCAGACGCTGCT
TGCCTGCGGCTACACGGTCGACGCCGTCTGCCCCGGAAAGAACGCAGGCGAGACGGTCGCCACCGCTATTCATGATTTCG
AAGGCGACCAGACCTATTCCGAAAAGCGCGGTCACAATTTCGCGCTGAACGCCACTTTCGACAGCGTGCGACCCGAAGAT
TACGACGCCCTCGTCATCCCAGGCGGCCGCGCGCCGGAATATCTGCGCCTCAATGCAGACGTCATCAAAGCGGTCCGCCA
CTTCTTCGATGCCGGAAAACCCGTTGCCGCCATCTGCCATGGCGCGCAGCTGCTCGCTGCCGCCGGCGTCTTGAAGGGCC
GCACATGCTCTGCCTATCCCGCCTGCCGGCCGGAAGTCGAGCTTGCCGGCGCCACCTTCGCCGATATCTCGATCACCGAT
GCGGTCTCGGATGGCAACCTGGTCACGGCGCCGGCCTGGCCGGCGCATCCGTCGTGGTTGCGCCAGTTCATGGCCGTGCT
CGATGCGGCAGCGCTGCTCGAAACCAACGCCGCCTGA

Upstream 100 bases:

>100_bases
TACTCTCACTCTCAGGGAATTTCGGCTGTAGTATTCCCATAGTACCTTCTGCATGCCGCTCTTTGTTAATCTCCGGCGGT
TGGTTTCAAAGGAGGATACG

Downstream 100 bases:

>100_bases
GGAGGCGGCACCGGGAGGATCACATGTGCGAATTGTTCATCAAGGCGGATGCGCGGCTCTGGGAAAGCACCACGCGGTCG
CTGCGCATCGACGGCATGGT

Product: intracellular protease PfpI family

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MPASKILMITGDFTEDYETMVPFQTLLACGYTVDAVCPGKNAGETVATAIHDFEGDQTYSEKRGHNFALNATFDSVRPED
YDALVIPGGRAPEYLRLNADVIKAVRHFFDAGKPVAAICHGAQLLAAAGVLKGRTCSAYPACRPEVELAGATFADISITD
AVSDGNLVTAPAWPAHPSWLRQFMAVLDAAALLETNAA

Sequences:

>Translated_198_residues
MPASKILMITGDFTEDYETMVPFQTLLACGYTVDAVCPGKNAGETVATAIHDFEGDQTYSEKRGHNFALNATFDSVRPED
YDALVIPGGRAPEYLRLNADVIKAVRHFFDAGKPVAAICHGAQLLAAAGVLKGRTCSAYPACRPEVELAGATFADISITD
AVSDGNLVTAPAWPAHPSWLRQFMAVLDAAALLETNAA
>Mature_197_residues
PASKILMITGDFTEDYETMVPFQTLLACGYTVDAVCPGKNAGETVATAIHDFEGDQTYSEKRGHNFALNATFDSVRPEDY
DALVIPGGRAPEYLRLNADVIKAVRHFFDAGKPVAAICHGAQLLAAAGVLKGRTCSAYPACRPEVELAGATFADISITDA
VSDGNLVTAPAWPAHPSWLRQFMAVLDAAALLETNAA

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=188, Percent_Identity=32.9787234042553, Blast_Score=95, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 21048; Mature: 20917

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASKILMITGDFTEDYETMVPFQTLLACGYTVDAVCPGKNAGETVATAIHDFEGDQTYS
CCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCHH
EKRGHNFALNATFDSVRPEDYDALVIPGGRAPEYLRLNADVIKAVRHFFDAGKPVAAICH
HHCCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHH
GAQLLAAAGVLKGRTCSAYPACRPEVELAGATFADISITDAVSDGNLVTAPAWPAHPSWL
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEECEEEEEECCCCCEEECCCCCCCHHHH
RQFMAVLDAAALLETNAA
HHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PASKILMITGDFTEDYETMVPFQTLLACGYTVDAVCPGKNAGETVATAIHDFEGDQTYS
CCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCCCHH
EKRGHNFALNATFDSVRPEDYDALVIPGGRAPEYLRLNADVIKAVRHFFDAGKPVAAICH
HHCCCEEEEECCCCCCCCCCCCEEEECCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHH
GAQLLAAAGVLKGRTCSAYPACRPEVELAGATFADISITDAVSDGNLVTAPAWPAHPSWL
HHHHHHHHHHHCCCCCCCCCCCCCCEEECCCEEECEEEEEECCCCCEEECCCCCCCHHHH
RQFMAVLDAAALLETNAA
HHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]