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The map label for this gene is ada [H]

Identifier: 209551015

GI number: 209551015

Start: 3554504

End: 3555376

Strand: Reverse

Name: ada [H]

Synonym: Rleg2_3439

Alternate gene names: 209551015

Gene position: 3555376-3554504 (Counterclockwise)

Preceding gene: 209551016

Following gene: 209551013

Centisome position: 78.35

GC content: 64.49

Gene sequence:

>873_bases
ATGAATATGATTGCGAACCTGCAGACAGACATCACGCCTGACGGCCCTGATTATGACATTGTCCGCCGGGTGATCGAACT
CATCACCGAGGATTACCGCGACCAGCCTTCGCTGGAGGTGATCGCGGCGCGGCTGAACCAGTCGCCGACCCAGCTGCAGA
AGACCTTCACCAGATGGGCCGGCCTGTCGCCCAAGGGTTTCCTGCAGGCGGTGACGCTCGACCACGCCAAGCGGCTGCTG
CGCCAGGAAGATATGCCGTTGCTGGAGACCTCGATCGAGGTCGGCCTCTCCGGGCCGAGCCGGCTGCATGATCTCTTCGT
CACCCATGAGGCGATGTCACCCGGCGAGTGGAAGGCCAAGGGTGGTGGTCTCACCATCCGCTACGGCTTCCACATCTGCC
CCTTCGGCATCGCGCTGATCATGGTGACCGACCGCGGCCTTGCCGGCCTTGCCTTCAGCGATTCCGGCGACGAGCGGGCC
TGCCTCGAGGATATGACCTGCCGCTGGCCGAATGCCCAATATGTCGAGGACCTGCAGGCGACCGTTCCCTACGCCGCCCG
CATCTTCGAGCCCGGCAAATGGTCTTCGGAACAGCCGTTGCGCGTCGTGCTGATCGGCACGGATTTCCAGGTGAGCGTCT
GGCAAAGCCTGATGAAAATCCCGTTCGGCAAGGCCGTCACCTATTCCGATATCGCCAAGGATATCGGCCGGCCGACGGCG
CAGCGCGCCGTGGGTGCGGCGGTCGGGGCCAACCCGATCTCCTTCGTGGTGCCATGCCATCGCGCGGTCGGCAAGAACGG
CGCGCTGACCGGTTACCATTGGGGCCTGACCCGCAAGCGGGCGATGCTCGGCTGGGAATCGGCGCACGCCTGA

Upstream 100 bases:

>100_bases
CACGGTCAAGCAGCGTATCTGAACTTAGTTCGCGGTGCGCAAGAAACGGGTGGTTTGCGAACAGCCAATTGATTATCTCC
TTATGACAAGGAGAAAGACG

Downstream 100 bases:

>100_bases
GGGGCGTTTTTCGTCAGGAGCGGCGGGTCGTTCGTCGCTTATCGCTCCGACCGTTCGCTCCTCGCGTCGCTCGCCCCCTC
ATCGCCTCGCTAACGCTCCG

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 290; Mature: 290

Protein sequence:

>290_residues
MNMIANLQTDITPDGPDYDIVRRVIELITEDYRDQPSLEVIAARLNQSPTQLQKTFTRWAGLSPKGFLQAVTLDHAKRLL
RQEDMPLLETSIEVGLSGPSRLHDLFVTHEAMSPGEWKAKGGGLTIRYGFHICPFGIALIMVTDRGLAGLAFSDSGDERA
CLEDMTCRWPNAQYVEDLQATVPYAARIFEPGKWSSEQPLRVVLIGTDFQVSVWQSLMKIPFGKAVTYSDIAKDIGRPTA
QRAVGAAVGANPISFVVPCHRAVGKNGALTGYHWGLTRKRAMLGWESAHA

Sequences:

>Translated_290_residues
MNMIANLQTDITPDGPDYDIVRRVIELITEDYRDQPSLEVIAARLNQSPTQLQKTFTRWAGLSPKGFLQAVTLDHAKRLL
RQEDMPLLETSIEVGLSGPSRLHDLFVTHEAMSPGEWKAKGGGLTIRYGFHICPFGIALIMVTDRGLAGLAFSDSGDERA
CLEDMTCRWPNAQYVEDLQATVPYAARIFEPGKWSSEQPLRVVLIGTDFQVSVWQSLMKIPFGKAVTYSDIAKDIGRPTA
QRAVGAAVGANPISFVVPCHRAVGKNGALTGYHWGLTRKRAMLGWESAHA
>Mature_290_residues
MNMIANLQTDITPDGPDYDIVRRVIELITEDYRDQPSLEVIAARLNQSPTQLQKTFTRWAGLSPKGFLQAVTLDHAKRLL
RQEDMPLLETSIEVGLSGPSRLHDLFVTHEAMSPGEWKAKGGGLTIRYGFHICPFGIALIMVTDRGLAGLAFSDSGDERA
CLEDMTCRWPNAQYVEDLQATVPYAARIFEPGKWSSEQPLRVVLIGTDFQVSVWQSLMKIPFGKAVTYSDIAKDIGRPTA
QRAVGAAVGANPISFVVPCHRAVGKNGALTGYHWGLTRKRAMLGWESAHA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=253, Percent_Identity=29.6442687747036, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787596, Length=78, Percent_Identity=55.1282051282051, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=45.7831325301205, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=46.3414634146341, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=87, Percent_Identity=50.5747126436782, Blast_Score=100, Evalue=3e-22,
Organism=Drosophila melanogaster, GI17137554, Length=172, Percent_Identity=36.046511627907, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31993; Mature: 31993

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMIANLQTDITPDGPDYDIVRRVIELITEDYRDQPSLEVIAARLNQSPTQLQKTFTRWA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHC
GLSPKGFLQAVTLDHAKRLLRQEDMPLLETSIEVGLSGPSRLHDLFVTHEAMSPGEWKAK
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEC
GGGLTIRYGFHICPFGIALIMVTDRGLAGLAFSDSGDERACLEDMTCRWPNAQYVEDLQA
CCCEEEEECCEECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHH
TVPYAARIFEPGKWSSEQPLRVVLIGTDFQVSVWQSLMKIPFGKAVTYSDIAKDIGRPTA
HCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHH
QRAVGAAVGANPISFVVPCHRAVGKNGALTGYHWGLTRKRAMLGWESAHA
HHHHHHHHCCCCEEEEEECHHHHCCCCCEEEEECCCHHHHHHHCCHHCCC
>Mature Secondary Structure
MNMIANLQTDITPDGPDYDIVRRVIELITEDYRDQPSLEVIAARLNQSPTQLQKTFTRWA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHC
GLSPKGFLQAVTLDHAKRLLRQEDMPLLETSIEVGLSGPSRLHDLFVTHEAMSPGEWKAK
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEEC
GGGLTIRYGFHICPFGIALIMVTDRGLAGLAFSDSGDERACLEDMTCRWPNAQYVEDLQA
CCCEEEEECCEECCCCEEEEEEECCCCEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHH
TVPYAARIFEPGKWSSEQPLRVVLIGTDFQVSVWQSLMKIPFGKAVTYSDIAKDIGRPTA
HCCHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHH
QRAVGAAVGANPISFVVPCHRAVGKNGALTGYHWGLTRKRAMLGWESAHA
HHHHHHHHCCCCEEEEEECHHHHCCCCCEEEEECCCHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904855; 11677609 [H]