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The map label for this gene is pecT [H]

Identifier: 209550895

GI number: 209550895

Start: 3417707

End: 3418561

Strand: Reverse

Name: pecT [H]

Synonym: Rleg2_3319

Alternate gene names: 209550895

Gene position: 3418561-3417707 (Counterclockwise)

Preceding gene: 209550897

Following gene: 209550893

Centisome position: 75.33

GC content: 65.15

Gene sequence:

>855_bases
ATGCTGGACCTTTCGCAACTGCGCAGTTTCGTCGCCGTCGAACAGATGGGCAGCTTTACGCTGGCGGCGGAAAGGCTCGG
CCTCGGCCAGTCGACGGTCAGCCAGCACATTCAGCGGCTGGAGGCGGCACTCGGCCGTAAGCTCCTGGCGCGTGACACGC
ATAAGGTCATTTTGACAGGCGATGGCGAGGCGCTGCTGTCGCATGCGCGCAGCATGCTTTCGATCGAAGGGCAGGTGCAG
TCGCTGTTTCGGGGCAACAGCCTGCGCGGCAGCCTGCGGCTCGGCGTGTCGGAGGATTTCGTCACCAGCCAGTTGCCGGC
GGTGCTGGAGGATTTCGTTCGTTCGCACCCCTCCGTCGATCTGGAGCTGACGGTGGCGCTATCTGGCGTGCTTTATGAGA
TGCAGGACAATGGCGAGATCGATCTAGTGCTTGCCAAGCGCCGGCTCGGCGATGCCCGCGGAAAACTCGTTTATCGCGAG
CCGCTCGTTTGGCTGGCGCGCGATCCCGAACGTGTGCTCGCCGCCGGCCCTTTGCCGCTGATCGCCTTTCCGCCGCCGAG
CGTCACACGGGCGATTGCGCTGGAAGCGCTGGCGCGAAACGGCGTGGCGTGGCGGATCGTCTGCACCTGTGGGAGCCTCA
GCGGGTTGACGGCGGCGGCGCGAGCCGGGATGGGCGTGCTGGTGCAGCCGCGCAGCATGGCGCCTTCAGGGCTGAAAGAG
ATCGCCGCGGCAAAACTTCCGCTGCTGGAGGATGTGGAATTCGTGCTGGTGCCGCGCAAAGGGGCAGATCAGGCGCTGGT
CGCCGCCCTTTCGGACGATATTCTGCAGAAAGTGAGACGGCTGAGGTCGGCGTGA

Upstream 100 bases:

>100_bases
AAACGGCGCATGACGGTCTCGCGGCGTGACAGATAGTCGTTGTTTTGTCGTCCATTATGATCCACGATGCAAGAAATAAC
AGTTATCAAGAAATTGGATC

Downstream 100 bases:

>100_bases
GCCGCGGCCGTCGCCGAAAGGGCTGCGGGAGGCAGGCCTCGGAAGGCGGTCAGGCGGCCAGGCACTTCAGGAAACCATCC
ACGTCCGCTTCTGTCGTCGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Pectinase gene transcriptional regulator [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MLDLSQLRSFVAVEQMGSFTLAAERLGLGQSTVSQHIQRLEAALGRKLLARDTHKVILTGDGEALLSHARSMLSIEGQVQ
SLFRGNSLRGSLRLGVSEDFVTSQLPAVLEDFVRSHPSVDLELTVALSGVLYEMQDNGEIDLVLAKRRLGDARGKLVYRE
PLVWLARDPERVLAAGPLPLIAFPPPSVTRAIALEALARNGVAWRIVCTCGSLSGLTAAARAGMGVLVQPRSMAPSGLKE
IAAAKLPLLEDVEFVLVPRKGADQALVAALSDDILQKVRRLRSA

Sequences:

>Translated_284_residues
MLDLSQLRSFVAVEQMGSFTLAAERLGLGQSTVSQHIQRLEAALGRKLLARDTHKVILTGDGEALLSHARSMLSIEGQVQ
SLFRGNSLRGSLRLGVSEDFVTSQLPAVLEDFVRSHPSVDLELTVALSGVLYEMQDNGEIDLVLAKRRLGDARGKLVYRE
PLVWLARDPERVLAAGPLPLIAFPPPSVTRAIALEALARNGVAWRIVCTCGSLSGLTAAARAGMGVLVQPRSMAPSGLKE
IAAAKLPLLEDVEFVLVPRKGADQALVAALSDDILQKVRRLRSA
>Mature_284_residues
MLDLSQLRSFVAVEQMGSFTLAAERLGLGQSTVSQHIQRLEAALGRKLLARDTHKVILTGDGEALLSHARSMLSIEGQVQ
SLFRGNSLRGSLRLGVSEDFVTSQLPAVLEDFVRSHPSVDLELTVALSGVLYEMQDNGEIDLVLAKRRLGDARGKLVYRE
PLVWLARDPERVLAAGPLPLIAFPPPSVTRAIALEALARNGVAWRIVCTCGSLSGLTAAARAGMGVLVQPRSMAPSGLKE
IAAAKLPLLEDVEFVLVPRKGADQALVAALSDDILQKVRRLRSA

Specific function: Regulates pectinase gene expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788626, Length=267, Percent_Identity=31.0861423220974, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788748, Length=278, Percent_Identity=29.4964028776978, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787806, Length=272, Percent_Identity=26.1029411764706, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1787589, Length=157, Percent_Identity=35.6687898089172, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1786448, Length=145, Percent_Identity=34.4827586206897, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790208, Length=148, Percent_Identity=34.4594594594595, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788297, Length=245, Percent_Identity=27.7551020408163, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI145693193, Length=253, Percent_Identity=28.4584980237154, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI2367136, Length=143, Percent_Identity=30.0699300699301, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=127, Percent_Identity=36.2204724409449, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1788887, Length=167, Percent_Identity=32.3353293413174, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=164, Percent_Identity=28.0487804878049, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788481, Length=239, Percent_Identity=26.3598326359833, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI157672245, Length=142, Percent_Identity=30.9859154929577, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 30538; Mature: 30538

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLSQLRSFVAVEQMGSFTLAAERLGLGQSTVSQHIQRLEAALGRKLLARDTHKVILTG
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
DGEALLSHARSMLSIEGQVQSLFRGNSLRGSLRLGVSEDFVTSQLPAVLEDFVRSHPSVD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
LELTVALSGVLYEMQDNGEIDLVLAKRRLGDARGKLVYREPLVWLARDPERVLAAGPLPL
EEEEEEEEEEEEEECCCCCEEEEEECHHCCHHHCCEEEECCEEEEECCHHHHHCCCCCCE
IAFPPPSVTRAIALEALARNGVAWRIVCTCGSLSGLTAAARAGMGVLVQPRSMAPSGLKE
EECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCEEEECCCCCCHHHHH
IAAAKLPLLEDVEFVLVPRKGADQALVAALSDDILQKVRRLRSA
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLDLSQLRSFVAVEQMGSFTLAAERLGLGQSTVSQHIQRLEAALGRKLLARDTHKVILTG
CCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
DGEALLSHARSMLSIEGQVQSLFRGNSLRGSLRLGVSEDFVTSQLPAVLEDFVRSHPSVD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
LELTVALSGVLYEMQDNGEIDLVLAKRRLGDARGKLVYREPLVWLARDPERVLAAGPLPL
EEEEEEEEEEEEEECCCCCEEEEEECHHCCHHHCCEEEECCEEEEECCHHHHHCCCCCCE
IAFPPPSVTRAIALEALARNGVAWRIVCTCGSLSGLTAAARAGMGVLVQPRSMAPSGLKE
EECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHCCCCEEEECCCCCCHHHHH
IAAAKLPLLEDVEFVLVPRKGADQALVAALSDDILQKVRRLRSA
HHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626286 [H]