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The map label for this gene is gltD [H]

Identifier: 209550892

GI number: 209550892

Start: 3409663

End: 3411138

Strand: Reverse

Name: gltD [H]

Synonym: Rleg2_3316

Alternate gene names: 209550892

Gene position: 3411138-3409663 (Counterclockwise)

Preceding gene: 209550893

Following gene: 209550889

Centisome position: 75.17

GC content: 64.16

Gene sequence:

>1476_bases
ATGAGGGTAAGGGACGAAGATATGGGTAAGGTAACAGGGTTTCTGGAAATCGACCGGCAAGTGGCGAAGTACCAGCCGGC
GTCGGATCGTATCCGCCATTTCCGTGAGTTCACGATCCCGATGTCGGACCCGGAAGTGCAGAAACAGGCTGCGCGCTGCA
TGGACTGTGGCATCCCCTATTGTCACGGCCCGACGGGCTGCCCTGTGCACAACCAGATCCCCGACTGGAACGACCTCGTC
TACAACAACAACTGGGAAGCAGCGATCCAGAACCTGCATTCGACCAACAACTTCCCGGAGTTCACCGGCCGCGTCTGCCC
GGCGCCCTGCGAGGAAGCCTGTACTTTGAATCTCGAAGATGCGCCTGTTGCCATCAAGACGGTCGAACAGGCGATCGCCG
ACAAGGCCTATGAGCTCGGCTTCATCCGGCCGCAGCCGGCCACGGTTCATACCGGCAAGAAGGTCGCGGTCATCGGCTCG
GGTCCCGCTGGCATGGCGGCTGCCCAGCAGCTCGGCCGTGCCGGCCACGAGGTGCATGTCTATGAGCGCGAGACCAAGCC
GGGCGGTCTGCTGCGCTATGGCATTCCCGACTTCAAGATGGAGAAGAACTTCATCGACCGCCGCGTCGAGCAGATGAAGG
GCGAGGGCGTCACCTTCCATTGCGGCGCCAATGTCGGCGTCGACGTCAAGGTCGAGCAGCTGCTCGCCGATCACGACGCC
GTCCTCTATTGCGGCGGCTCCGAGACGCCACGCGAGGCAGGCATTCCAGGCACGGATCTTGCCGGCGTGCATGATGCCAT
GCCCTATCTGGTGCAGCAGAACCGCCGCGTCGGGCGCGAGAACATCGACAGCGTCGGCTGGCCGTCGGACCCGATCCTTG
CCGGCGCCAAACATATCGTCGTCGTCGGCGGCGGCGACACGGCTTCGGACTGCGTCGGCACGGCGTTTCGCCAGGGCGCC
GTCAAGGTCACCCAGCTCGACATCCGGCCGCAGCCGCCGGAGAAGGAGGACAAGCTTGCTGTCTGGCCGTTCTGGGCAAC
GAAGATGCGCACCTCCTCCTCGCAGGCTGAGGGCGCCGTGCGCGAATTCCAGGTGGCGACACTCGAATTCGTTGGCGAAG
ACGGCGTGTTGACCGGGGTCAAGTGCTGCGAGGTCGACGAGCGCCGGCGGCCGGTTCCAGGCACGGAATTCGTCATCCGC
GCCGATCTCGCCTTCATCGCCATCGGTTTCCGCGGCCCGTTCACGACAAGCGTGCTGAAGGAACTCGAGGGAAAGCTGAC
GCTCAACACTGACAAGCGCGGCTCGACCAATGTCGTCGCCAACGACCGCGACTACAAGACCTCGGTCGATAAGTTCTGGA
CGGCAGGCGACGTGCGCCGCGGCCAGTCGCTGGTGGTCTGGGCAATCCGCGAAGGCCGCCAGGCGGCGCGCGCCATCGAC
GAGGCATTGATGGGCTCGACCGTTCTGCCGAACTGA

Upstream 100 bases:

>100_bases
GCGGATGTTTCAGAGGACATGTTCCAATGACGGTCACGAGAAGCAACTTGCCCCGGTTACCGGGACTGACGATAGACAGA
TTGGCTGCGGTGAGGCGGTC

Downstream 100 bases:

>100_bases
CCGCAAGCCGCGTAGAGATCCCGAGACAAAACTCCGCCCTTCCGGCGGAGTTTTTTGTTTGGGAAACGTTCTCGGTGCGA
CCGCTTGACGCGCCGGTATC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MRVRDEDMGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDPEVQKQAARCMDCGIPYCHGPTGCPVHNQIPDWNDLV
YNNNWEAAIQNLHSTNNFPEFTGRVCPAPCEEACTLNLEDAPVAIKTVEQAIADKAYELGFIRPQPATVHTGKKVAVIGS
GPAGMAAAQQLGRAGHEVHVYERETKPGGLLRYGIPDFKMEKNFIDRRVEQMKGEGVTFHCGANVGVDVKVEQLLADHDA
VLYCGGSETPREAGIPGTDLAGVHDAMPYLVQQNRRVGRENIDSVGWPSDPILAGAKHIVVVGGGDTASDCVGTAFRQGA
VKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVATLEFVGEDGVLTGVKCCEVDERRRPVPGTEFVIR
ADLAFIAIGFRGPFTTSVLKELEGKLTLNTDKRGSTNVVANDRDYKTSVDKFWTAGDVRRGQSLVVWAIREGRQAARAID
EALMGSTVLPN

Sequences:

>Translated_491_residues
MRVRDEDMGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDPEVQKQAARCMDCGIPYCHGPTGCPVHNQIPDWNDLV
YNNNWEAAIQNLHSTNNFPEFTGRVCPAPCEEACTLNLEDAPVAIKTVEQAIADKAYELGFIRPQPATVHTGKKVAVIGS
GPAGMAAAQQLGRAGHEVHVYERETKPGGLLRYGIPDFKMEKNFIDRRVEQMKGEGVTFHCGANVGVDVKVEQLLADHDA
VLYCGGSETPREAGIPGTDLAGVHDAMPYLVQQNRRVGRENIDSVGWPSDPILAGAKHIVVVGGGDTASDCVGTAFRQGA
VKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVATLEFVGEDGVLTGVKCCEVDERRRPVPGTEFVIR
ADLAFIAIGFRGPFTTSVLKELEGKLTLNTDKRGSTNVVANDRDYKTSVDKFWTAGDVRRGQSLVVWAIREGRQAARAID
EALMGSTVLPN
>Mature_491_residues
MRVRDEDMGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDPEVQKQAARCMDCGIPYCHGPTGCPVHNQIPDWNDLV
YNNNWEAAIQNLHSTNNFPEFTGRVCPAPCEEACTLNLEDAPVAIKTVEQAIADKAYELGFIRPQPATVHTGKKVAVIGS
GPAGMAAAQQLGRAGHEVHVYERETKPGGLLRYGIPDFKMEKNFIDRRVEQMKGEGVTFHCGANVGVDVKVEQLLADHDA
VLYCGGSETPREAGIPGTDLAGVHDAMPYLVQQNRRVGRENIDSVGWPSDPILAGAKHIVVVGGGDTASDCVGTAFRQGA
VKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAVREFQVATLEFVGEDGVLTGVKCCEVDERRRPVPGTEFVIR
ADLAFIAIGFRGPFTTSVLKELEGKLTLNTDKRGSTNVVANDRDYKTSVDKFWTAGDVRRGQSLVVWAIREGRQAARAID
EALMGSTVLPN

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=330, Percent_Identity=27.5757575757576, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI111118983, Length=234, Percent_Identity=28.6324786324786, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI111118981, Length=233, Percent_Identity=27.4678111587983, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1789606, Length=487, Percent_Identity=37.3716632443532, Blast_Score=261, Evalue=5e-71,
Organism=Escherichia coli, GI1788811, Length=466, Percent_Identity=38.412017167382, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI87082180, Length=479, Percent_Identity=32.776617954071, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788468, Length=457, Percent_Identity=27.5711159737418, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789243, Length=455, Percent_Identity=29.010989010989, Blast_Score=118, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=511, Percent_Identity=42.0743639921722, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI71984108, Length=305, Percent_Identity=24.5901639344262, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17543860, Length=169, Percent_Identity=34.3195266272189, Blast_Score=81, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=502, Percent_Identity=38.6454183266932, Blast_Score=372, Evalue=1e-104,
Organism=Drosophila melanogaster, GI28574881, Length=498, Percent_Identity=44.578313253012, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24665539, Length=498, Percent_Identity=44.578313253012, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI24665547, Length=498, Percent_Identity=44.578313253012, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24665543, Length=498, Percent_Identity=44.578313253012, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24640763, Length=477, Percent_Identity=24.7379454926625, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18858217, Length=477, Percent_Identity=24.7379454926625, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53798; Mature: 53798

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRDEDMGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDPEVQKQAARCMDCGIPY
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
CHGPTGCPVHNQIPDWNDLVYNNNWEAAIQNLHSTNNFPEFTGRVCPAPCEEACTLNLED
CCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHCCCCCCCCCCHHEEECCCC
APVAIKTVEQAIADKAYELGFIRPQPATVHTGKKVAVIGSGPAGMAAAQQLGRAGHEVHV
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHCCCCEEEE
YERETKPGGLLRYGIPDFKMEKNFIDRRVEQMKGEGVTFHCGANVGVDVKVEQLLADHDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHCCCCE
VLYCGGSETPREAGIPGTDLAGVHDAMPYLVQQNRRVGRENIDSVGWPSDPILAGAKHIV
EEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEE
VVGGGDTASDCVGTAFRQGAVKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAV
EEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHHCCCCCHHHHHH
REFQVATLEFVGEDGVLTGVKCCEVDERRRPVPGTEFVIRADLAFIAIGFRGPFTTSVLK
HHHEEEEEEEECCCCCEECCEEECCHHHCCCCCCCCEEEEECEEEEEEECCCCHHHHHHH
ELEGKLTLNTDKRGSTNVVANDRDYKTSVDKFWTAGDVRRGQSLVVWAIREGRQAARAID
HHCCCEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHH
EALMGSTVLPN
HHHHCCCCCCC
>Mature Secondary Structure
MRVRDEDMGKVTGFLEIDRQVAKYQPASDRIRHFREFTIPMSDPEVQKQAARCMDCGIPY
CCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
CHGPTGCPVHNQIPDWNDLVYNNNWEAAIQNLHSTNNFPEFTGRVCPAPCEEACTLNLED
CCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCCHHCCCCCCCCCCHHEEECCCC
APVAIKTVEQAIADKAYELGFIRPQPATVHTGKKVAVIGSGPAGMAAAQQLGRAGHEVHV
CCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCHHHHHHHHCCCCEEEE
YERETKPGGLLRYGIPDFKMEKNFIDRRVEQMKGEGVTFHCGANVGVDVKVEQLLADHDA
EECCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEHHHHHCCCCE
VLYCGGSETPREAGIPGTDLAGVHDAMPYLVQQNRRVGRENIDSVGWPSDPILAGAKHIV
EEEECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCEEE
VVGGGDTASDCVGTAFRQGAVKVTQLDIRPQPPEKEDKLAVWPFWATKMRTSSSQAEGAV
EEECCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCEEEEEEHHHHHCCCCCHHHHHH
REFQVATLEFVGEDGVLTGVKCCEVDERRRPVPGTEFVIRADLAFIAIGFRGPFTTSVLK
HHHEEEEEEEECCCCCEECCEEECCHHHCCCCCCCCEEEEECEEEEEEECCCCHHHHHHH
ELEGKLTLNTDKRGSTNVVANDRDYKTSVDKFWTAGDVRRGQSLVVWAIREGRQAARAID
HHCCCEEEECCCCCCCCEEECCCCCHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHH
EALMGSTVLPN
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]