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The map label for this gene is galE [C]

Identifier: 209550782

GI number: 209550782

Start: 3294626

End: 3295846

Strand: Direct

Name: galE [C]

Synonym: Rleg2_3206

Alternate gene names: 209550782

Gene position: 3294626-3295846 (Clockwise)

Preceding gene: 209550767

Following gene: 209550783

Centisome position: 72.6

GC content: 60.93

Gene sequence:

>1221_bases
ATGAAGATTGCGGTTATGGGCGGAGACGGTTTCATTGGCTGGCCAACGTCGCTGCACCTCTCCGATGCCGGGCATGACGT
TCATATCCTCGACAATCTCTCACGCCGCTGGATCGACACGGAACTTGGCGTTCAGTCGCTGACACCGATGGATTCGATCC
AGGAGCGCACCCGCATCTGGCATGCCGAAACCGGACGCCGCATCCACTTCAATCTGATCGATCTCGCCAAGGATTACGAA
CTGCTGAAGAAATGGCTTTCCGAACACCGCCCGGATGCCGTCATCCATTTCGCCGAGCAGCGGGCCGCGCCCTATTCGAT
GAAAAGCGACCGCCACAAGAATTACACGGTTAACAACAATGTCAGCGCCACGCACAACCTGCTTAACGCGCTGACCGAGC
TCAATCTCGACGCCCATCTCATCCACCTCGGCACCATGGGCGTCTACGGCTATTCGACTGTGGGCGCAGCGATCCCCGAA
GGCTACCTGCCGGTCGGCATCGAAACCGCAGCCGGCGAGACTGTCAGCCAGGAGATCCTCTATCCCTCCAATCCCGGCTC
GATCTACCACATGACCAAGTGCCTGGATCAGCTCCTCTTCCAGTTCTACGCCAAGAACGACGGATTGAGGATCACCGACC
TGCACCAGGGCATCGTCTGGGGCACGCATACCGAACAGACGCGCCGCCATGCGCAGCTGATCAACCGTTTCGATTACGAC
GGCGATTACGGCACCGTGCTGAACCGCTTCCTCATCCAAGCGGCGATCGGCTACCCGCTGACGGTGCACGGCACCGGCGG
CCAGACCCGCGCCTTCATCCATATCCAGGATTCGGTACGCTGCATCGAACTGGCGCTCAAGAATCCGCCCGCCCGCGGCG
CCCGCGTTGAAATCTTCAACCAGATGACAGAAACCCACCGGGTGCGCGACCTCGCCCAGATGATCGCGGGGATAAGCGGC
GCCGACATCGCCTGGCTGCCCAACCCGCGCAAGGAAGCCGCCGAGAACGAGCTGATCGTCCAGAACGAGAAATTCCGCAT
TCTCGGCCTCGAGCCGATCACGCTGGAAGCAGGCCTTCTCGGCGAGATCGTCGACGTGGCGAAGAAATTCGCCTATCGCG
TCGACCGCTCGCGCGTGCCGGCCGTGTCCGCCTGGACAAAGGACATCGCCGCGACGATCAATCACGACCCGGAAGGCAAG
CGGCTGAAGTCGGTGTCATGA

Upstream 100 bases:

>100_bases
ACCGGCAGGCAGGCATTGAATGCCCAGCCGGTAGGCATTGAATGATTGTCATAATTCCTAACTATTCCCGATATCGAGCG
ACAGATCAGGAGACGATGTG

Downstream 100 bases:

>100_bases
GTGATGGTTTGCTACTGAAGCGCCGCCAATCCAACGATCTCTCCCTCATTCCTGTGCTTGTCACAGGAATCCAGCGCGCC
CAAGTCTTTGGGCGCAGGAG

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NAD-Dependent Epimerase/Dehydratase; Sulfolipid Biosynthesis Protein; NAD Dependent Epimerase/Dehydratase Family; Sulfolipid Biosynthesis Protein SqdB; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase; UDP Sulfoquinovose Synthase; NAD Dependent Epimerase/Dehydratase Family Protein

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MKIAVMGGDGFIGWPTSLHLSDAGHDVHILDNLSRRWIDTELGVQSLTPMDSIQERTRIWHAETGRRIHFNLIDLAKDYE
LLKKWLSEHRPDAVIHFAEQRAAPYSMKSDRHKNYTVNNNVSATHNLLNALTELNLDAHLIHLGTMGVYGYSTVGAAIPE
GYLPVGIETAAGETVSQEILYPSNPGSIYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHAQLINRFDYD
GDYGTVLNRFLIQAAIGYPLTVHGTGGQTRAFIHIQDSVRCIELALKNPPARGARVEIFNQMTETHRVRDLAQMIAGISG
ADIAWLPNPRKEAAENELIVQNEKFRILGLEPITLEAGLLGEIVDVAKKFAYRVDRSRVPAVSAWTKDIAATINHDPEGK
RLKSVS

Sequences:

>Translated_406_residues
MKIAVMGGDGFIGWPTSLHLSDAGHDVHILDNLSRRWIDTELGVQSLTPMDSIQERTRIWHAETGRRIHFNLIDLAKDYE
LLKKWLSEHRPDAVIHFAEQRAAPYSMKSDRHKNYTVNNNVSATHNLLNALTELNLDAHLIHLGTMGVYGYSTVGAAIPE
GYLPVGIETAAGETVSQEILYPSNPGSIYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHAQLINRFDYD
GDYGTVLNRFLIQAAIGYPLTVHGTGGQTRAFIHIQDSVRCIELALKNPPARGARVEIFNQMTETHRVRDLAQMIAGISG
ADIAWLPNPRKEAAENELIVQNEKFRILGLEPITLEAGLLGEIVDVAKKFAYRVDRSRVPAVSAWTKDIAATINHDPEGK
RLKSVS
>Mature_406_residues
MKIAVMGGDGFIGWPTSLHLSDAGHDVHILDNLSRRWIDTELGVQSLTPMDSIQERTRIWHAETGRRIHFNLIDLAKDYE
LLKKWLSEHRPDAVIHFAEQRAAPYSMKSDRHKNYTVNNNVSATHNLLNALTELNLDAHLIHLGTMGVYGYSTVGAAIPE
GYLPVGIETAAGETVSQEILYPSNPGSIYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHAQLINRFDYD
GDYGTVLNRFLIQAAIGYPLTVHGTGGQTRAFIHIQDSVRCIELALKNPPARGARVEIFNQMTETHRVRDLAQMIAGISG
ADIAWLPNPRKEAAENELIVQNEKFRILGLEPITLEAGLLGEIVDVAKKFAYRVDRSRVPAVSAWTKDIAATINHDPEGK
RLKSVS

Specific function: Galactose metabolism; third step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI42516563, Length=387, Percent_Identity=24.031007751938, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 45449; Mature: 45449

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVMGGDGFIGWPTSLHLSDAGHDVHILDNLSRRWIDTELGVQSLTPMDSIQERTRIW
CEEEEECCCCEECCCCEEEECCCCCEEEEHHHHHHHHCCHHCCHHHCCCHHHHHHHHHEE
HAETGRRIHFNLIDLAKDYELLKKWLSEHRPDAVIHFAEQRAAPYSMKSDRHKNYTVNNN
ECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCEEECCC
VSATHNLLNALTELNLDAHLIHLGTMGVYGYSTVGAAIPEGYLPVGIETAAGETVSQEIL
CHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCEECCCCCCHHHCCC
YPSNPGSIYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHAQLINRFDYD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCHHHHHHHHHHHHHCCCC
GDYGTVLNRFLIQAAIGYPLTVHGTGGQTRAFIHIQDSVRCIELALKNPPARGARVEIFN
CCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEHHHCCCCCCCCHHHHHH
QMTETHRVRDLAQMIAGISGADIAWLPNPRKEAAENELIVQNEKFRILGLEPITLEAGLL
HHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCCEEEECCCEEEEECCCEEECCHHH
GEIVDVAKKFAYRVDRSRVPAVSAWTKDIAATINHDPEGKRLKSVS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIAVMGGDGFIGWPTSLHLSDAGHDVHILDNLSRRWIDTELGVQSLTPMDSIQERTRIW
CEEEEECCCCEECCCCEEEECCCCCEEEEHHHHHHHHCCHHCCHHHCCCHHHHHHHHHEE
HAETGRRIHFNLIDLAKDYELLKKWLSEHRPDAVIHFAEQRAAPYSMKSDRHKNYTVNNN
ECCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCCCCCCCEEECCC
VSATHNLLNALTELNLDAHLIHLGTMGVYGYSTVGAAIPEGYLPVGIETAAGETVSQEIL
CHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCCEECCCCCCHHHCCC
YPSNPGSIYHMTKCLDQLLFQFYAKNDGLRITDLHQGIVWGTHTEQTRRHAQLINRFDYD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCHHHHHHHHHHHHHCCCC
GDYGTVLNRFLIQAAIGYPLTVHGTGGQTRAFIHIQDSVRCIELALKNPPARGARVEIFN
CCHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEECCCCEEEEHHHCCCCCCCCHHHHHH
QMTETHRVRDLAQMIAGISGADIAWLPNPRKEAAENELIVQNEKFRILGLEPITLEAGLL
HHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHCCCCEEEECCCEEEEECCCEEECCHHH
GEIVDVAKKFAYRVDRSRVPAVSAWTKDIAATINHDPEGKRLKSVS
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA