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The map label for this gene is tolB [H]

Identifier: 209550776

GI number: 209550776

Start: 3289508

End: 3290815

Strand: Reverse

Name: tolB [H]

Synonym: Rleg2_3200

Alternate gene names: 209550776

Gene position: 3290815-3289508 (Counterclockwise)

Preceding gene: 209550777

Following gene: 209550775

Centisome position: 72.52

GC content: 62.08

Gene sequence:

>1308_bases
ATGGTCAAGTGTTCCCTCATCCGCGCTCTGATGGTCATTGCAGGCCTGATTGGGGCTGCAGCCTTTACGACCCCGGCGAA
CGCGTTGGTCACCCTCGACCTTCGAAAAGGCAATGTCCAGCCGATGCCGATTGCGGTGACCGACTTCCAGGGTGACCTGG
GCGCGCAGGTCTCGCAAGTCATCGCCGCCGACCTGCAGCGTTCCGGTCTGTTTGCCCCGATCAACAAGACGGCCTTTATC
GAAAAGATTTCCAATCCGGATGCTGCCCCGCGTTTCGAGGACTGGAAGGTCATCAATGCACAGGCACTGGTTACCGGTCG
GGTTACCAAGGAAGCGGATGGCCGCCTTCGCGCCGAATTCCGGCTCTGGGACCCGTTCGCCGGACAGCAGATGACCGGTC
AGCAGTTCTATACGCAGCCGGAGAACTGGCGCCGTGTGGCTCACATCATCGCCGATGCGATCTACAAGCAGATCACCGGC
GAAGAGGGTTATTTCGACACCCGCGTGGTCTTCGTCTCCGAATCCGGCACCAAGCAGCAGCGCAAGCGGCAGCTCGCGAT
CATGGACCAGGACGGCTTCAACGTGCGTATGCTGACGGATGGCAGCGATCTCGTGCTGACGCCGCGCTTCTCGCCGAGCC
GGCAGGAAGTCACCTACATGTCCTTTGCCAACCAGCAGCCGCGGGTCTACCTGCTGCAGCTCGAAACCGGGCAGCGTGAA
GTCGTCGGCAATTTCCCCGGCATGACCTTCTCGCCGCGCTTTTCGCCGGATGGTCAGAAGGTGATCATGAGCCTGCAGCA
GGACGCCAATTCCAACATCTATACGATGGACCTGCGCTCGCGCACGACGACGCGTCTGACCTCGACGGCTGCGATCGACA
CGTCGCCTTCCTATTCCCCCGACGGCGCGCGTGTCAGCTTCGAAAGCGACCGCGGCGGAAAACCGCAGATCTATGTGATG
AATGCCGATGGTTCCGGCCAGACCCGTATTTCCTTCGGTGACGGCTCCTATTCGACGCCGGTCTGGTCGCCGCGCGGCGA
TCTCATCGCCTTCACCAAGCAGGCCGGCGGCAAGTTCTCGATCGGCGTGATGAAACCGGATGGTTCCGGCGAGCGCATTC
TGACGACCGGCTTCCACAATGAGGGCCCGACCTGGGCGCCGAACGGCCGCGTGCTGATGTTCTTCCGCCAGGCGGCAGGT
GCCGGCGGGCCGCAGCTTTATTCGATCGATCTGACCGGCTACAACGAGCAGCTTGTGAAGACGCCGAGCTACGGTTCCGA
CCCGGCCTGGTCGCCGCTGATGGAGTAG

Upstream 100 bases:

>100_bases
TACAATGCTTCCAAAAGATAAATACGATGGCTGGAAGGAAGTCATTTTGAACTTCGACACCAGCGCTCTTACCCATTAGA
TGCTGAAAGGCTTGTTTGAT

Downstream 100 bases:

>100_bases
GCGATGCGCTGCTGCCTTCATCTCGTCCCAAAGTCATGAAATCGAGCGACCAAGGGACAAATCAGTAATTCGTTAACCAT
AATTTTTGAGGGGCGGTTAA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MVKCSLIRALMVIAGLIGAAAFTTPANALVTLDLRKGNVQPMPIAVTDFQGDLGAQVSQVIAADLQRSGLFAPINKTAFI
EKISNPDAAPRFEDWKVINAQALVTGRVTKEADGRLRAEFRLWDPFAGQQMTGQQFYTQPENWRRVAHIIADAIYKQITG
EEGYFDTRVVFVSESGTKQQRKRQLAIMDQDGFNVRMLTDGSDLVLTPRFSPSRQEVTYMSFANQQPRVYLLQLETGQRE
VVGNFPGMTFSPRFSPDGQKVIMSLQQDANSNIYTMDLRSRTTTRLTSTAAIDTSPSYSPDGARVSFESDRGGKPQIYVM
NADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQAGGKFSIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQAAG
AGGPQLYSIDLTGYNEQLVKTPSYGSDPAWSPLME

Sequences:

>Translated_435_residues
MVKCSLIRALMVIAGLIGAAAFTTPANALVTLDLRKGNVQPMPIAVTDFQGDLGAQVSQVIAADLQRSGLFAPINKTAFI
EKISNPDAAPRFEDWKVINAQALVTGRVTKEADGRLRAEFRLWDPFAGQQMTGQQFYTQPENWRRVAHIIADAIYKQITG
EEGYFDTRVVFVSESGTKQQRKRQLAIMDQDGFNVRMLTDGSDLVLTPRFSPSRQEVTYMSFANQQPRVYLLQLETGQRE
VVGNFPGMTFSPRFSPDGQKVIMSLQQDANSNIYTMDLRSRTTTRLTSTAAIDTSPSYSPDGARVSFESDRGGKPQIYVM
NADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQAGGKFSIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQAAG
AGGPQLYSIDLTGYNEQLVKTPSYGSDPAWSPLME
>Mature_435_residues
MVKCSLIRALMVIAGLIGAAAFTTPANALVTLDLRKGNVQPMPIAVTDFQGDLGAQVSQVIAADLQRSGLFAPINKTAFI
EKISNPDAAPRFEDWKVINAQALVTGRVTKEADGRLRAEFRLWDPFAGQQMTGQQFYTQPENWRRVAHIIADAIYKQITG
EEGYFDTRVVFVSESGTKQQRKRQLAIMDQDGFNVRMLTDGSDLVLTPRFSPSRQEVTYMSFANQQPRVYLLQLETGQRE
VVGNFPGMTFSPRFSPDGQKVIMSLQQDANSNIYTMDLRSRTTTRLTSTAAIDTSPSYSPDGARVSFESDRGGKPQIYVM
NADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQAGGKFSIGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQAAG
AGGPQLYSIDLTGYNEQLVKTPSYGSDPAWSPLME

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=437, Percent_Identity=27.2311212814645, Blast_Score=164, Evalue=9e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 47779; Mature: 47779

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKCSLIRALMVIAGLIGAAAFTTPANALVTLDLRKGNVQPMPIAVTDFQGDLGAQVSQV
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHH
IAADLQRSGLFAPINKTAFIEKISNPDAAPRFEDWKVINAQALVTGRVTKEADGRLRAEF
HHHHHHHCCCCCCCCCCEEEHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEE
RLWDPFAGQQMTGQQFYTQPENWRRVAHIIADAIYKQITGEEGYFDTRVVFVSESGTKQQ
EEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHH
RKRQLAIMDQDGFNVRMLTDGSDLVLTPRFSPSRQEVTYMSFANQQPRVYLLQLETGQRE
HHCEEEEEECCCCEEEEEECCCCEEEECCCCCCHHEEEEEECCCCCCCEEEEEECCCCEE
VVGNFPGMTFSPRFSPDGQKVIMSLQQDANSNIYTMDLRSRTTTRLTSTAAIDTSPSYSP
EEECCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCC
DGARVSFESDRGGKPQIYVMNADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQAGGKFS
CCCEEEECCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEE
IGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQAAGAGGPQLYSIDLTGYNEQLVK
EEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCHHEEE
TPSYGSDPAWSPLME
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MVKCSLIRALMVIAGLIGAAAFTTPANALVTLDLRKGNVQPMPIAVTDFQGDLGAQVSQV
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHH
IAADLQRSGLFAPINKTAFIEKISNPDAAPRFEDWKVINAQALVTGRVTKEADGRLRAEF
HHHHHHHCCCCCCCCCCEEEHHHCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCEEEEE
RLWDPFAGQQMTGQQFYTQPENWRRVAHIIADAIYKQITGEEGYFDTRVVFVSESGTKQQ
EEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCHHH
RKRQLAIMDQDGFNVRMLTDGSDLVLTPRFSPSRQEVTYMSFANQQPRVYLLQLETGQRE
HHCEEEEEECCCCEEEEEECCCCEEEECCCCCCHHEEEEEECCCCCCCEEEEEECCCCEE
VVGNFPGMTFSPRFSPDGQKVIMSLQQDANSNIYTMDLRSRTTTRLTSTAAIDTSPSYSP
EEECCCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEEEEEEEEECCCCCCCC
DGARVSFESDRGGKPQIYVMNADGSGQTRISFGDGSYSTPVWSPRGDLIAFTKQAGGKFS
CCCEEEECCCCCCCCEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEE
IGVMKPDGSGERILTTGFHNEGPTWAPNGRVLMFFRQAAGAGGPQLYSIDLTGYNEQLVK
EEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCHHEEE
TPSYGSDPAWSPLME
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA