BacMap

Definition	Rhizobium leguminosarum bv. trifolii WSM2304 chromosome, complete genome.
Accession	NC_011369
Length	4,537,948

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The map label for this gene is braF [H]

Identifier: 209550762

GI number: 209550762

Start: 3272734

End: 3273498

Strand: Direct

Name: braF [H]

Synonym: Rleg2_3186

Alternate gene names: 209550762

Gene position: 3272734-3273498 (Clockwise)

Preceding gene: 209550761

Following gene: 209550763

Centisome position: 72.12

GC content: 63.4

Gene sequence:

>765_bases
ATGAGCCCGGTCTTCGAAGTCGCCAATCTCAAAAAAGCCTTCGGCGGCCTTGCCGTCACCAATGATGTCTCACTGGCGAT
GTCGCCGGGCGATCGTGTGGCGCTGATCGGTCCGAACGGGGCCGGCAAGACCACTTTCGTCAACCTGGTGACCGGCAACC
TTCCCCCCGATTCCGGCGAGGTGCGGCTTGGCGGCGAGGTGGTCACGAAAGTCGGCGCGATCGGCAGGGTCAAACGCGGT
CTGGTGCGATCCTTCCAGGTGACACGGCTTTTCCAGGACATGACACCGGCCGAACATGTCGGGCTCGCCGTTCTTCAGCG
GGACGGACGCAGCGGACGCATGTTCGGCAATTTCCTGCATATGCCCGAGGTCATGGCGGAGGTCGACGATCTCCTGGGAA
AGCTCGGGCTGGCGCCGCTGATGCATCGCAAGGTCAGCGAGATCGCCTATGGCCAGCAGCGGCTTCTCGAAATCGCCGTG
GCGCTGGCGCTAAAGCCGAAAGTGCTGCTGCTCGACGAGCCGGCAGCCGGCGTGCCGCAAAGCGATACCGGCCGCATCGA
GCAGGCGCTGGCCGATCTGCCTGCCGATCTCGCCGTCCTGATGATCGAACACGATATGGATCTTGTCTTCCGTTTTGCCA
GACGCGTCATCGTGCTGGCGGCCGGCGCGATCATCTTCGATGGCCGGCCTGAAGACGTCACCAAGGATGCCCGCGTGCGC
GAGGCTTATCTCGGGAGCTATGCCAATGCCAGCCCTGTCGCTTGA

Upstream 100 bases:

>100_bases
CCATGGTCGGCGCGCTGTTGATTGCCGTCGTGCTTTTCGCGCCGAAAGGTCTCTACGGGACCGCCGCGAGCCTTATCGGC
CGCCGCAAGGAGCAGCGGTC

Downstream 100 bases:

>100_bases
GGTCGAAAATCTCTCGGCCGGATATGGGCCGACGCGCGTGCTCGAAGACATTTCCTTCTCCATACCGGCGGGCGCCCGGC
TTGCCGTGCTCGGCCGCAAC

Product: ABC transporter

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSPVFEVANLKKAFGGLAVTNDVSLAMSPGDRVALIGPNGAGKTTFVNLVTGNLPPDSGEVRLGGEVVTKVGAIGRVKRG
LVRSFQVTRLFQDMTPAEHVGLAVLQRDGRSGRMFGNFLHMPEVMAEVDDLLGKLGLAPLMHRKVSEIAYGQQRLLEIAV
ALALKPKVLLLDEPAAGVPQSDTGRIEQALADLPADLAVLMIEHDMDLVFRFARRVIVLAAGAIIFDGRPEDVTKDARVR
EAYLGSYANASPVA

Sequences:

>Translated_254_residues
MSPVFEVANLKKAFGGLAVTNDVSLAMSPGDRVALIGPNGAGKTTFVNLVTGNLPPDSGEVRLGGEVVTKVGAIGRVKRG
LVRSFQVTRLFQDMTPAEHVGLAVLQRDGRSGRMFGNFLHMPEVMAEVDDLLGKLGLAPLMHRKVSEIAYGQQRLLEIAV
ALALKPKVLLLDEPAAGVPQSDTGRIEQALADLPADLAVLMIEHDMDLVFRFARRVIVLAAGAIIFDGRPEDVTKDARVR
EAYLGSYANASPVA
>Mature_253_residues
SPVFEVANLKKAFGGLAVTNDVSLAMSPGDRVALIGPNGAGKTTFVNLVTGNLPPDSGEVRLGGEVVTKVGAIGRVKRGL
VRSFQVTRLFQDMTPAEHVGLAVLQRDGRSGRMFGNFLHMPEVMAEVDDLLGKLGLAPLMHRKVSEIAYGQQRLLEIAVA
LALKPKVLLLDEPAAGVPQSDTGRIEQALADLPADLAVLMIEHDMDLVFRFARRVIVLAAGAIIFDGRPEDVTKDARVRE
AYLGSYANASPVA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI105990541, Length=226, Percent_Identity=30.0884955752212, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116734710, Length=224, Percent_Identity=29.9107142857143, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI27881501, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI30795238, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI150417984, Length=233, Percent_Identity=31.3304721030043, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI21536376, Length=223, Percent_Identity=30.4932735426009, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI47078218, Length=210, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI45446740, Length=210, Percent_Identity=25.7142857142857, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI31657092, Length=224, Percent_Identity=26.3392857142857, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI27477115, Length=224, Percent_Identity=26.3392857142857, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI27436953, Length=224, Percent_Identity=23.2142857142857, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI27262624, Length=205, Percent_Identity=23.9024390243902, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI27262626, Length=205, Percent_Identity=23.9024390243902, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1789864, Length=254, Percent_Identity=36.2204724409449, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1788761, Length=239, Percent_Identity=33.8912133891213, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87081709, Length=251, Percent_Identity=31.4741035856574, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI1789593, Length=242, Percent_Identity=33.0578512396694, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI87081791, Length=250, Percent_Identity=26.8, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1787370, Length=240, Percent_Identity=28.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787029, Length=237, Percent_Identity=29.535864978903, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1790190, Length=243, Percent_Identity=27.5720164609054, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788450, Length=231, Percent_Identity=26.8398268398268, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789672, Length=247, Percent_Identity=26.7206477732794, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790544, Length=241, Percent_Identity=26.1410788381743, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790739, Length=243, Percent_Identity=27.9835390946502, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788225, Length=235, Percent_Identity=28.0851063829787, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788897, Length=258, Percent_Identity=27.5193798449612, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789991, Length=228, Percent_Identity=26.7543859649123, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1790525, Length=243, Percent_Identity=25.1028806584362, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI48995001, Length=227, Percent_Identity=26.431718061674, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1786563, Length=226, Percent_Identity=27.8761061946903, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786872, Length=236, Percent_Identity=27.5423728813559, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1790162, Length=257, Percent_Identity=28.4046692607004, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788210, Length=223, Percent_Identity=25.5605381165919, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787792, Length=237, Percent_Identity=24.4725738396624, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788165, Length=240, Percent_Identity=28.75, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1789891, Length=211, Percent_Identity=30.3317535545024, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787089, Length=221, Percent_Identity=27.1493212669683, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693107, Length=242, Percent_Identity=31.404958677686, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1786253, Length=232, Percent_Identity=27.5862068965517, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082268, Length=237, Percent_Identity=25.3164556962025, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786345, Length=262, Percent_Identity=25.5725190839695, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786398, Length=230, Percent_Identity=29.5652173913043, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI2367384, Length=215, Percent_Identity=27.4418604651163, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=26.8722466960352, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI87081834, Length=224, Percent_Identity=25.8928571428571, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787712, Length=255, Percent_Identity=24.7058823529412, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI48994943, Length=253, Percent_Identity=24.901185770751, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081782, Length=238, Percent_Identity=26.0504201680672, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786319, Length=228, Percent_Identity=27.1929824561404, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789873, Length=217, Percent_Identity=27.1889400921659, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788472, Length=223, Percent_Identity=23.3183856502242, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1788506, Length=222, Percent_Identity=27.027027027027, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787500, Length=240, Percent_Identity=25.8333333333333, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI48994997, Length=250, Percent_Identity=24, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786803, Length=244, Percent_Identity=23.7704918032787, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1789586, Length=236, Percent_Identity=29.2372881355932, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789032, Length=262, Percent_Identity=24.0458015267176, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788002, Length=225, Percent_Identity=27.5555555555556, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787759, Length=259, Percent_Identity=27.027027027027, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1789751, Length=236, Percent_Identity=23.728813559322, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786698, Length=224, Percent_Identity=24.1071428571429, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786703, Length=223, Percent_Identity=27.3542600896861, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1788644, Length=243, Percent_Identity=28.8065843621399, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=229, Percent_Identity=27.9475982532751, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983510, Length=229, Percent_Identity=27.9475982532751, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983523, Length=229, Percent_Identity=27.9475982532751, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=223, Percent_Identity=27.3542600896861, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17510237, Length=232, Percent_Identity=22.8448275862069, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24666092, Length=219, Percent_Identity=27.3972602739726, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24643674, Length=224, Percent_Identity=27.6785714285714, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221512771, Length=223, Percent_Identity=26.0089686098655, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28574150, Length=218, Percent_Identity=28.8990825688073, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007328, Length=218, Percent_Identity=28.8990825688073, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI221500365, Length=221, Percent_Identity=28.0542986425339, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI116007184, Length=221, Percent_Identity=28.0542986425339, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648314, Length=223, Percent_Identity=26.0089686098655, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581191, Length=212, Percent_Identity=27.3584905660377, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581189, Length=212, Percent_Identity=27.3584905660377, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581193, Length=212, Percent_Identity=27.3584905660377, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581195, Length=212, Percent_Identity=27.3584905660377, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI28571209, Length=207, Percent_Identity=26.0869565217391, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24659289, Length=218, Percent_Identity=24.7706422018349, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 27118; Mature: 26987

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVFEVANLKKAFGGLAVTNDVSLAMSPGDRVALIGPNGAGKTTFVNLVTGNLPPDSGE
CCCHHHHHHHHHHHCCEEEECCCEEEECCCCEEEEECCCCCCCEEEEEEEECCCCCCCCC
VRLGGEVVTKVGAIGRVKRGLVRSFQVTRLFQDMTPAEHVGLAVLQRDGRSGRMFGNFLH
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHH
MPEVMAEVDDLLGKLGLAPLMHRKVSEIAYGQQRLLEIAVALALKPKVLLLDEPAAGVPQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
SDTGRIEQALADLPADLAVLMIEHDMDLVFRFARRVIVLAAGAIIFDGRPEDVTKDARVR
CCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHH
EAYLGSYANASPVA
HHHHCCCCCCCCCC
>Mature Secondary Structure 
SPVFEVANLKKAFGGLAVTNDVSLAMSPGDRVALIGPNGAGKTTFVNLVTGNLPPDSGE
CCHHHHHHHHHHHCCEEEECCCEEEECCCCEEEEECCCCCCCEEEEEEEECCCCCCCCC
VRLGGEVVTKVGAIGRVKRGLVRSFQVTRLFQDMTPAEHVGLAVLQRDGRSGRMFGNFLH
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHH
MPEVMAEVDDLLGKLGLAPLMHRKVSEIAYGQQRLLEIAVALALKPKVLLLDEPAAGVPQ
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCC
SDTGRIEQALADLPADLAVLMIEHDMDLVFRFARRVIVLAAGAIIFDGRPEDVTKDARVR
CCHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHH
EAYLGSYANASPVA
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]