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The map label for this gene is pit [H]

Identifier: 209550754

GI number: 209550754

Start: 3261819

End: 3263420

Strand: Reverse

Name: pit [H]

Synonym: Rleg2_3178

Alternate gene names: 209550754

Gene position: 3263420-3261819 (Counterclockwise)

Preceding gene: 209550757

Following gene: 209550750

Centisome position: 71.91

GC content: 62.67

Gene sequence:

>1602_bases
ATGGCCGATATTGCCCCCAGCCAGGTTCATAGCGACGCTTCCCACCCGCTCCACAGCTCGAATTCATCAGGCAAATGGTT
CTTGCCGCTCTTCGCGCTCGTTCTGGTCTGCGGTCTTGGATATGTCGCCTATGCGCTCGGACGCGACCTGACAACCGCAG
TTGCGGTTCCCTGGATTCTCCTGGGCCTTGCGCTGCTGATCGCGCTCGGCTTCGAATTCGTCAACGGTTTCCACGATACG
GCCAATGCCGTCGCCACCGTCATTTACACCCGTTCGATGCCTGCCGAATTCGCCGTCATCTGGTCGGGCTTCTTTAACTT
CCTCGGGGTGCTGACCTCGAGCGGCGCCGTCGCTTTCGGCATTCTGGCGCTGCTGCCGGTGGAACTCATCCTGCAGGTCG
GCTCCGGTTCCGGCCTTGCCATGGTCTTCGCGCTGCTGATCGCCGCGATCGTCTGGAATCTCGGCACCTGGTATCTTGGC
CTGCCGTCGTCGAGCTCGCATACGCTTGTGGGTTCGATCATCGGTGTCGGCCTCGCCAACCAGTTTCTCGCGCCGGCAGG
CACGGCGACGAGCGGCGTCGACTGGTCGCAGGCGACCAATATCGGCCTGTCGCTGCTGATCTCGCCACTGATCGGCTTCG
GTCTTTCCGCTGTTCTGCTGCTGGTGATGAAGCTTCTGGTGCGCAACAAGGCGCTCTATGCCGAACCGAAGGGCAACCAG
CCGCCGCCGCTCTGGATCCGCGCGATCCTGATCTGCACCTGCACCGGCGTCAGCTTCGCCCACGGCTCCAACGACGGCCA
GAAGGGCATGGGTCTGATCATGCTCATCCTGATCGGCCTGGTGCCGACGGCCTTCGCGCTGAACCGCACGCCGGATGTGA
ACTATCTCGAAGCCTACAAGTCGGCATCGGTCCAGGTCGAAACCGCGCTCGCCAAATATGTGAGGCCGGGTGTCGCCGTC
ACCGACTACAAGGCCGAGGTCAGCAACGCCGTCAAGAACAAGACCTGGACGGATGCGACGACACCGGCCCTGCAGCAATA
TATCCATCAGACAAGCGCCGAAGTCGCCGCCTTCCCGACGCTCGAAGCGGTGCCGACCAATCTCGTCGGCAACGTCCGCA
ACGACATCTACCTGATCGGCGAGGCGCTGAAGCTGATCGACAAGCAGAAGCTGCTGCCGATGGATGCCGGCGATCTCTCG
GCGGTCACGAACTACCACAAGGCAGTCGACAATGCGACGAAGTTCATCCCCCTCTGGGTGAAGGTGGCGGTCGCGATCGC
GCTTGGCCTCGGCACGATGGTGGGCTGGAAGCGCATCGTCGTCACCGTCGGCGAAAAGATCGGCAAGACGCATCTGACGT
ACGGTCAGGGCGCGGCAGCCGAAGTCGTGGCGATGGTCACCATCGCTTCGGCCGATCACCTCGGCCTGCCGGTCTCGACC
ACGCATGTCCTGTCGTCAGGCGTCGCCGGCACCATGGCGGCGAACGGTTCCGGCCTGCAATGGTCGACCGTGCGCAACAT
GCTGATGGCCTGGGTGCTGACGCTGCCGGCATCGATCGCGATCGCCTTCGTGCTCTTCGTGATCCTGCGCCAGGTATTTT
AA

Upstream 100 bases:

>100_bases
CAGTGCCTTAGATGGCGAACCAGACAGGCGTCATGAGAGCTTCACGCTGCGGAATTATTAACAAAAGGCAAATTTGCAAA
ACGGAATAAGGAACTATGCC

Downstream 100 bases:

>100_bases
AAACGCAGCGTCAGGCCGTGGAGGCCAGACTGGCATTGCGATCGCTGATGAAGATGCCCGCCTCGGCGGCGGGCATCGCC
TTGCCGAAAAGATAACCTTG

Product: phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MADIAPSQVHSDASHPLHSSNSSGKWFLPLFALVLVCGLGYVAYALGRDLTTAVAVPWILLGLALLIALGFEFVNGFHDT
ANAVATVIYTRSMPAEFAVIWSGFFNFLGVLTSSGAVAFGILALLPVELILQVGSGSGLAMVFALLIAAIVWNLGTWYLG
LPSSSSHTLVGSIIGVGLANQFLAPAGTATSGVDWSQATNIGLSLLISPLIGFGLSAVLLLVMKLLVRNKALYAEPKGNQ
PPPLWIRAILICTCTGVSFAHGSNDGQKGMGLIMLILIGLVPTAFALNRTPDVNYLEAYKSASVQVETALAKYVRPGVAV
TDYKAEVSNAVKNKTWTDATTPALQQYIHQTSAEVAAFPTLEAVPTNLVGNVRNDIYLIGEALKLIDKQKLLPMDAGDLS
AVTNYHKAVDNATKFIPLWVKVAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAEVVAMVTIASADHLGLPVST
THVLSSGVAGTMAANGSGLQWSTVRNMLMAWVLTLPASIAIAFVLFVILRQVF

Sequences:

>Translated_533_residues
MADIAPSQVHSDASHPLHSSNSSGKWFLPLFALVLVCGLGYVAYALGRDLTTAVAVPWILLGLALLIALGFEFVNGFHDT
ANAVATVIYTRSMPAEFAVIWSGFFNFLGVLTSSGAVAFGILALLPVELILQVGSGSGLAMVFALLIAAIVWNLGTWYLG
LPSSSSHTLVGSIIGVGLANQFLAPAGTATSGVDWSQATNIGLSLLISPLIGFGLSAVLLLVMKLLVRNKALYAEPKGNQ
PPPLWIRAILICTCTGVSFAHGSNDGQKGMGLIMLILIGLVPTAFALNRTPDVNYLEAYKSASVQVETALAKYVRPGVAV
TDYKAEVSNAVKNKTWTDATTPALQQYIHQTSAEVAAFPTLEAVPTNLVGNVRNDIYLIGEALKLIDKQKLLPMDAGDLS
AVTNYHKAVDNATKFIPLWVKVAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAEVVAMVTIASADHLGLPVST
THVLSSGVAGTMAANGSGLQWSTVRNMLMAWVLTLPASIAIAFVLFVILRQVF
>Mature_532_residues
ADIAPSQVHSDASHPLHSSNSSGKWFLPLFALVLVCGLGYVAYALGRDLTTAVAVPWILLGLALLIALGFEFVNGFHDTA
NAVATVIYTRSMPAEFAVIWSGFFNFLGVLTSSGAVAFGILALLPVELILQVGSGSGLAMVFALLIAAIVWNLGTWYLGL
PSSSSHTLVGSIIGVGLANQFLAPAGTATSGVDWSQATNIGLSLLISPLIGFGLSAVLLLVMKLLVRNKALYAEPKGNQP
PPLWIRAILICTCTGVSFAHGSNDGQKGMGLIMLILIGLVPTAFALNRTPDVNYLEAYKSASVQVETALAKYVRPGVAVT
DYKAEVSNAVKNKTWTDATTPALQQYIHQTSAEVAAFPTLEAVPTNLVGNVRNDIYLIGEALKLIDKQKLLPMDAGDLSA
VTNYHKAVDNATKFIPLWVKVAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAEVVAMVTIASADHLGLPVSTT
HVLSSGVAGTMAANGSGLQWSTVRNMLMAWVLTLPASIAIAFVLFVILRQVF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789907, Length=483, Percent_Identity=41.2008281573499, Blast_Score=338, Evalue=8e-94,
Organism=Escherichia coli, GI1789360, Length=507, Percent_Identity=38.4615384615385, Blast_Score=336, Evalue=2e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 56103; Mature: 55972

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIAPSQVHSDASHPLHSSNSSGKWFLPLFALVLVCGLGYVAYALGRDLTTAVAVPWIL
CCCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGLALLIALGFEFVNGFHDTANAVATVIYTRSMPAEFAVIWSGFFNFLGVLTSSGAVAFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
ILALLPVELILQVGSGSGLAMVFALLIAAIVWNLGTWYLGLPSSSSHTLVGSIIGVGLAN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHH
QFLAPAGTATSGVDWSQATNIGLSLLISPLIGFGLSAVLLLVMKLLVRNKALYAEPKGNQ
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PPPLWIRAILICTCTGVSFAHGSNDGQKGMGLIMLILIGLVPTAFALNRTPDVNYLEAYK
CCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SASVQVETALAKYVRPGVAVTDYKAEVSNAVKNKTWTDATTPALQQYIHQTSAEVAAFPT
CCCHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
LEAVPTNLVGNVRNDIYLIGEALKLIDKQKLLPMDAGDLSAVTNYHKAVDNATKFIPLWV
HHHCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KVAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAEVVAMVTIASADHLGLPVST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
THVLSSGVAGTMAANGSGLQWSTVRNMLMAWVLTLPASIAIAFVLFVILRQVF
HHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ADIAPSQVHSDASHPLHSSNSSGKWFLPLFALVLVCGLGYVAYALGRDLTTAVAVPWIL
CCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LGLALLIALGFEFVNGFHDTANAVATVIYTRSMPAEFAVIWSGFFNFLGVLTSSGAVAFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHH
ILALLPVELILQVGSGSGLAMVFALLIAAIVWNLGTWYLGLPSSSSHTLVGSIIGVGLAN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHH
QFLAPAGTATSGVDWSQATNIGLSLLISPLIGFGLSAVLLLVMKLLVRNKALYAEPKGNQ
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCC
PPPLWIRAILICTCTGVSFAHGSNDGQKGMGLIMLILIGLVPTAFALNRTPDVNYLEAYK
CCHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SASVQVETALAKYVRPGVAVTDYKAEVSNAVKNKTWTDATTPALQQYIHQTSAEVAAFPT
CCCHHHHHHHHHHHCCCCEEECHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
LEAVPTNLVGNVRNDIYLIGEALKLIDKQKLLPMDAGDLSAVTNYHKAVDNATKFIPLWV
HHHCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KVAVAIALGLGTMVGWKRIVVTVGEKIGKTHLTYGQGAAAEVVAMVTIASADHLGLPVST
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHH
THVLSSGVAGTMAANGSGLQWSTVRNMLMAWVLTLPASIAIAFVLFVILRQVF
HHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10993077 [H]