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The map label for this gene is hipO [H]

Identifier: 209550741

GI number: 209550741

Start: 3248767

End: 3249936

Strand: Reverse

Name: hipO [H]

Synonym: Rleg2_3165

Alternate gene names: 209550741

Gene position: 3249936-3248767 (Counterclockwise)

Preceding gene: 209550742

Following gene: 209550723

Centisome position: 71.62

GC content: 64.62

Gene sequence:

>1170_bases
ATGAGCATCCCCGCCCGGATCAAGGACGATCTGCCGTTCCTAACCTCTTTGCGCCGCGATCTGCATGCCCATCCCGAACT
CGGTTTCGAGGAGGAGCGCACTGCCAGCATCGTCGCGAGGCTTCTCGAGGAGGCTGATATCACGGTGCATCGCGGGCTGG
CCGGCACCGGCGTCGTCGGCGCGCTCACTGTCGGCAACGGCACGCGCAGGATCGGGCTCAGGGCCGATATGGATGCGCTC
GCCATGCCTGAAATGGCGGAGCGGGCTTATAAATCGACGGTACCCGGAAAGATGCATGCCTGCGGCCATGACGGCCATAC
GGCGACGCTTCTCGGCGCCGCACGATATCTTGCCGCGACGCGGGGTTTTTCGGGCACGGTGCATTTCATCTTCCAGCCGG
CCGAAGAGGGCCGTGGCGGCGCGAAGCGCATGGTGGAGGAGGGGCTGTTTAAGCTTTTCCCCTGTGACGCCGTTTACGGG
CTGCACAATATGCCGGGGCTATCGGTCGATGAGATCGCCGTGGTCGAGGGACCGCAGCTCGCCTCTTCCGACAGCTGGCG
CATCACCTTTCGCGGCATCGGCACGCACGGCGCCAAGCCGCATCTCGGCCGCGATCCGATCACCGCCGCCGCCACCTTCC
TGTCATCGCTGCAGACGATCGTCGGGCGAGTGGTCGATCCGCTGCAGCCGGCCGTCGTCAGCGCCTGTTTCCTGCAGGCG
GGCGACCCGAAGGCTTTGAATGTCATTCCCGATATCGTGGAGATCGGCGGAACGGCACGGGCCTATTCGCCGGATGTGCG
CGATCAGCTGGAGAGCGAGATCGGTCGGCTGGCCAGTGGAACGGCCGCCATGTACGGCATATCGGCCGATTATCGTTTCG
AGCGGCGCATTCCGCCCGTCGTCAATGATGGGGATGCGACCGCCCGAGCGCTTGCCGCCGCCGGCACGGTGTTCGGCGGG
AAGGTCCGGACGAGCTTTCCGCCGTCGACAGCAGGCGACGACTTTGCCTTCTTCGCGCAGAATGCGCCAGGCTGCTACGT
CTGGCTCGGCAATGGGCCGGCAGTGGACGGGGCGCTGCATCACAACACCGCTTATGATTTCAATGATGAGGCGCTTGGGT
ATGGGGCGGCTTATTGGGTGGCGTTGGTGGAACGGGAGTTGAAGGGTTAG

Upstream 100 bases:

>100_bases
GAGACTTGATGGTTGAACTTGCGGGCTGTTTGATCAACAAAGGATGATCTGACGAGGGCGCCGATGGGCGCCCGCTACGC
TTGACAGGATATCCAGGCTC

Downstream 100 bases:

>100_bases
CACCACGTTTGTGGCCCCCTCATCCGCCCTGCGGGCACCTTCTCCCCGCTGGGGAGAAGGGACTTGTAGCAACGTCTCGA
TTCCCCTCCTACCCCTCGGG

Product: amidohydrolase

Products: NA

Alternate protein names: Benzoylglycine amidohydrolase; Hippuricase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSIPARIKDDLPFLTSLRRDLHAHPELGFEEERTASIVARLLEEADITVHRGLAGTGVVGALTVGNGTRRIGLRADMDAL
AMPEMAERAYKSTVPGKMHACGHDGHTATLLGAARYLAATRGFSGTVHFIFQPAEEGRGGAKRMVEEGLFKLFPCDAVYG
LHNMPGLSVDEIAVVEGPQLASSDSWRITFRGIGTHGAKPHLGRDPITAAATFLSSLQTIVGRVVDPLQPAVVSACFLQA
GDPKALNVIPDIVEIGGTARAYSPDVRDQLESEIGRLASGTAAMYGISADYRFERRIPPVVNDGDATARALAAAGTVFGG
KVRTSFPPSTAGDDFAFFAQNAPGCYVWLGNGPAVDGALHHNTAYDFNDEALGYGAAYWVALVERELKG

Sequences:

>Translated_389_residues
MSIPARIKDDLPFLTSLRRDLHAHPELGFEEERTASIVARLLEEADITVHRGLAGTGVVGALTVGNGTRRIGLRADMDAL
AMPEMAERAYKSTVPGKMHACGHDGHTATLLGAARYLAATRGFSGTVHFIFQPAEEGRGGAKRMVEEGLFKLFPCDAVYG
LHNMPGLSVDEIAVVEGPQLASSDSWRITFRGIGTHGAKPHLGRDPITAAATFLSSLQTIVGRVVDPLQPAVVSACFLQA
GDPKALNVIPDIVEIGGTARAYSPDVRDQLESEIGRLASGTAAMYGISADYRFERRIPPVVNDGDATARALAAAGTVFGG
KVRTSFPPSTAGDDFAFFAQNAPGCYVWLGNGPAVDGALHHNTAYDFNDEALGYGAAYWVALVERELKG
>Mature_388_residues
SIPARIKDDLPFLTSLRRDLHAHPELGFEEERTASIVARLLEEADITVHRGLAGTGVVGALTVGNGTRRIGLRADMDALA
MPEMAERAYKSTVPGKMHACGHDGHTATLLGAARYLAATRGFSGTVHFIFQPAEEGRGGAKRMVEEGLFKLFPCDAVYGL
HNMPGLSVDEIAVVEGPQLASSDSWRITFRGIGTHGAKPHLGRDPITAAATFLSSLQTIVGRVVDPLQPAVVSACFLQAG
DPKALNVIPDIVEIGGTARAYSPDVRDQLESEIGRLASGTAAMYGISADYRFERRIPPVVNDGDATARALAAAGTVFGGK
VRTSFPPSTAGDDFAFFAQNAPGCYVWLGNGPAVDGALHHNTAYDFNDEALGYGAAYWVALVERELKG

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=426, Percent_Identity=27.4647887323944, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787598, Length=304, Percent_Identity=27.6315789473684, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.32 [H]

Molecular weight: Translated: 41284; Mature: 41153

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPARIKDDLPFLTSLRRDLHAHPELGFEEERTASIVARLLEEADITVHRGLAGTGVVG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCEE
ALTVGNGTRRIGLRADMDALAMPEMAERAYKSTVPGKMHACGHDGHTATLLGAARYLAAT
EEEECCCCEEECEECCCHHHCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHH
RGFSGTVHFIFQPAEEGRGGAKRMVEEGLFKLFPCDAVYGLHNMPGLSVDEIAVVEGPQL
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEECCCCC
ASSDSWRITFRGIGTHGAKPHLGRDPITAAATFLSSLQTIVGRVVDPLQPAVVSACFLQA
CCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
GDPKALNVIPDIVEIGGTARAYSPDVRDQLESEIGRLASGTAAMYGISADYRFERRIPPV
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCEECCCCCCC
VNDGDATARALAAAGTVFGGKVRTSFPPSTAGDDFAFFAQNAPGCYVWLGNGPAVDGALH
CCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEE
HNTAYDFNDEALGYGAAYWVALVERELKG
CCCCCCCCCCHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIPARIKDDLPFLTSLRRDLHAHPELGFEEERTASIVARLLEEADITVHRGLAGTGVVG
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCCCEE
ALTVGNGTRRIGLRADMDALAMPEMAERAYKSTVPGKMHACGHDGHTATLLGAARYLAAT
EEEECCCCEEECEECCCHHHCCHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHH
RGFSGTVHFIFQPAEEGRGGAKRMVEEGLFKLFPCDAVYGLHNMPGLSVDEIAVVEGPQL
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCEEEECCCCC
ASSDSWRITFRGIGTHGAKPHLGRDPITAAATFLSSLQTIVGRVVDPLQPAVVSACFLQA
CCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
GDPKALNVIPDIVEIGGTARAYSPDVRDQLESEIGRLASGTAAMYGISADYRFERRIPPV
CCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHEEECCCCCEECCCCCCC
VNDGDATARALAAAGTVFGGKVRTSFPPSTAGDDFAFFAQNAPGCYVWLGNGPAVDGALH
CCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCEEE
HNTAYDFNDEALGYGAAYWVALVERELKG
CCCCCCCCCCHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7730270; 10688204 [H]