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The map label for this gene is wcaL [H]

Identifier: 209550539

GI number: 209550539

Start: 3023343

End: 3024494

Strand: Direct

Name: wcaL [H]

Synonym: Rleg2_2963

Alternate gene names: 209550539

Gene position: 3023343-3024494 (Clockwise)

Preceding gene: 209550538

Following gene: 209550540

Centisome position: 66.62

GC content: 59.2

Gene sequence:

>1152_bases
ATGAGAAAAGCCGTTATTTACGTGGAAAAATTTCTGCCTGCCAGCCAGGCATTTGTTCTCAATCAGGCGGTGGCATTTCG
TTCCTTCGAAGCTGAAATTCTTGCCGGTTCGCGAATTTCTTCCGCCCATACCAAAAAATCCACGGTTCCGGTTCATGACA
TCCGTCGGTCTCCCGTTGCGCGGGCCGGTGAACTGCTTCTGAAAATCCCCCAGATCGGCCTTCCCTTTCTCTTTCCCGCG
ATCGGCAATGCCGATGTTATCCACGCCCATTTCGGCAAGAACGGTTATGTCATCGGGCCCCTGGCGCGCGCCGCCGGCAA
GCCGCTGGTCACGACATTCCACGGCTTCGACGCCACCTATGGCGGCGATCCAAAAAAGCCGGGCGGCTTCAACCAGGTGC
GCTTCTTTGCCAAGGGACGCCGGGAGATGGCCGGCTGGAACAGCTGGAATATCGCCGTTTCCGATTTCATCCGCGACCGG
CTGCTGGCGCTCGGCTTTCGCGCCGATCGGGTCTTTCGCCACCATATCGGCATCGATCTCGACCTCTTCAAAATGGAACC
GCGTCCGCGAAAAAAAGGCCTGGTGGTTTCCATTGCCCGTTTCGTCGACTATAAGGGCCACCGCTTCATGATCGACGCGC
TGTCGCGCGTGGCGGCGACCGGCACCCCGGTCGAATTCGTCATGGTGGGTCAGGGACCGCTGAAAGAGGAAATCGAGGCG
CTGGCGCGTCGTTCCTTGCCAAGCGTCACGATCCATGAAAATCTGTCGCAGACTGAGATAAGGGATCTGCTCGCCAGTGC
GGAGCTTTATCTTCATGGCAGCGTGACCCTCGACAATGGTCACGCCGAAGCGTTCGGCCTCGCCAATCTAGAGGCGGAAG
CCGTCGGGACTCCGGTCGTCGCATTTCGCTCGGGAGGCGTGGGCGAAGCGATCGAAGAGGGGAAAACTGGTTATCTCGTG
GAGGAGCGGGATGTCGCAGGAATGGCCGAAGCCGTCGGAAGGCTGCTCAACGACCAGTCCCTGTGGACAGCCTTCAGCGC
GCGGGCACCGCTTCTGGTGGCCGAGCGCTTCGACTTACGTCGTCAGACGGGGATGCTCGAGGACTATTACAGTTCCGTGC
TAGACGAATTTTCCAGCCGGGGTCGGACTTGA

Upstream 100 bases:

>100_bases
TACGACGAAAACAACACGCCGAGAAGTGCGGTTGATGGCATCCGACGGGCAATAGAGGCGATGTGATGCGTAAGAAAACA
ACGGAGACGTCGACAGATTC

Downstream 100 bases:

>100_bases
TGGTGCAGACAGGAAAGAAGCCGAAGTGGGGACTGAACGTATTTCGGCCGCTGCGGAACAGCATAGTGACGGCCAAGTGG
CTCTACTATACGAAATTCTG

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRKAVIYVEKFLPASQAFVLNQAVAFRSFEAEILAGSRISSAHTKKSTVPVHDIRRSPVARAGELLLKIPQIGLPFLFPA
IGNADVIHAHFGKNGYVIGPLARAAGKPLVTTFHGFDATYGGDPKKPGGFNQVRFFAKGRREMAGWNSWNIAVSDFIRDR
LLALGFRADRVFRHHIGIDLDLFKMEPRPRKKGLVVSIARFVDYKGHRFMIDALSRVAATGTPVEFVMVGQGPLKEEIEA
LARRSLPSVTIHENLSQTEIRDLLASAELYLHGSVTLDNGHAEAFGLANLEAEAVGTPVVAFRSGGVGEAIEEGKTGYLV
EERDVAGMAEAVGRLLNDQSLWTAFSARAPLLVAERFDLRRQTGMLEDYYSSVLDEFSSRGRT

Sequences:

>Translated_383_residues
MRKAVIYVEKFLPASQAFVLNQAVAFRSFEAEILAGSRISSAHTKKSTVPVHDIRRSPVARAGELLLKIPQIGLPFLFPA
IGNADVIHAHFGKNGYVIGPLARAAGKPLVTTFHGFDATYGGDPKKPGGFNQVRFFAKGRREMAGWNSWNIAVSDFIRDR
LLALGFRADRVFRHHIGIDLDLFKMEPRPRKKGLVVSIARFVDYKGHRFMIDALSRVAATGTPVEFVMVGQGPLKEEIEA
LARRSLPSVTIHENLSQTEIRDLLASAELYLHGSVTLDNGHAEAFGLANLEAEAVGTPVVAFRSGGVGEAIEEGKTGYLV
EERDVAGMAEAVGRLLNDQSLWTAFSARAPLLVAERFDLRRQTGMLEDYYSSVLDEFSSRGRT
>Mature_383_residues
MRKAVIYVEKFLPASQAFVLNQAVAFRSFEAEILAGSRISSAHTKKSTVPVHDIRRSPVARAGELLLKIPQIGLPFLFPA
IGNADVIHAHFGKNGYVIGPLARAAGKPLVTTFHGFDATYGGDPKKPGGFNQVRFFAKGRREMAGWNSWNIAVSDFIRDR
LLALGFRADRVFRHHIGIDLDLFKMEPRPRKKGLVVSIARFVDYKGHRFMIDALSRVAATGTPVEFVMVGQGPLKEEIEA
LARRSLPSVTIHENLSQTEIRDLLASAELYLHGSVTLDNGHAEAFGLANLEAEAVGTPVVAFRSGGVGEAIEEGKTGYLV
EERDVAGMAEAVGRLLNDQSLWTAFSARAPLLVAERFDLRRQTGMLEDYYSSVLDEFSSRGRT

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1788357, Length=373, Percent_Identity=25.4691689008043, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 42061; Mature: 42061

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAVIYVEKFLPASQAFVLNQAVAFRSFEAEILAGSRISSAHTKKSTVPVHDIRRSPVA
CCCEEEEHHHHCCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCCHHHHHCCCHH
RAGELLLKIPQIGLPFLFPAIGNADVIHAHFGKNGYVIGPLARAAGKPLVTTFHGFDATY
HHHHHHEECCCCCCCCHHHCCCCCCEEEEEECCCCEEEECHHHHCCCCEEEEECCCCCCC
GGDPKKPGGFNQVRFFAKGRREMAGWNSWNIAVSDFIRDRLLALGFRADRVFRHHIGIDL
CCCCCCCCCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCE
DLFKMEPRPRKKGLVVSIARFVDYKGHRFMIDALSRVAATGTPVEFVMVGQGPLKEEIEA
EEEECCCCCCCCCHHEEHHHHHCCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
LARRSLPSVTIHENLSQTEIRDLLASAELYLHGSVTLDNGHAEAFGLANLEAEAVGTPVV
HHHHCCCCEEECCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCEEEECCCCHHHCCCCEE
AFRSGGVGEAIEEGKTGYLVEERDVAGMAEAVGRLLNDQSLWTAFSARAPLLVAERFDLR
EEECCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEHHHHHHH
RQTGMLEDYYSSVLDEFSSRGRT
HHHCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKAVIYVEKFLPASQAFVLNQAVAFRSFEAEILAGSRISSAHTKKSTVPVHDIRRSPVA
CCCEEEEHHHHCCCHHHHHHHHHHHHHCCCHHHEECCCCCCCCCCCCCCCHHHHHCCCHH
RAGELLLKIPQIGLPFLFPAIGNADVIHAHFGKNGYVIGPLARAAGKPLVTTFHGFDATY
HHHHHHEECCCCCCCCHHHCCCCCCEEEEEECCCCEEEECHHHHCCCCEEEEECCCCCCC
GGDPKKPGGFNQVRFFAKGRREMAGWNSWNIAVSDFIRDRLLALGFRADRVFRHHIGIDL
CCCCCCCCCHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCE
DLFKMEPRPRKKGLVVSIARFVDYKGHRFMIDALSRVAATGTPVEFVMVGQGPLKEEIEA
EEEECCCCCCCCCHHEEHHHHHCCCCCEEHHHHHHHHHCCCCCEEEEEECCCCCHHHHHH
LARRSLPSVTIHENLSQTEIRDLLASAELYLHGSVTLDNGHAEAFGLANLEAEAVGTPVV
HHHHCCCCEEECCCCCHHHHHHHHHHCEEEEEEEEEECCCCCCEEEECCCCHHHCCCCEE
AFRSGGVGEAIEEGKTGYLVEERDVAGMAEAVGRLLNDQSLWTAFSARAPLLVAERFDLR
EEECCCCCHHHHCCCCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEHHHHHHH
RQTGMLEDYYSSVLDEFSSRGRT
HHHCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]