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The map label for this gene is glpK [H]

Identifier: 209550438

GI number: 209550438

Start: 2909024

End: 2910517

Strand: Reverse

Name: glpK [H]

Synonym: Rleg2_2859

Alternate gene names: 209550438

Gene position: 2910517-2909024 (Counterclockwise)

Preceding gene: 209550443

Following gene: 209550437

Centisome position: 64.14

GC content: 65.19

Gene sequence:

>1494_bases
ATGGGTGGATATGTTCTGGCGATCGATCAGGGGACGACGTCGACGCGGGCGATCGTCTTCGATGGCGACATGCATGTCGC
CGGCAAGGGCCAGAAGGAATTCACCCAGATCTATCCTCGCTCCGGCTGGGTCGAGCATGATCCCGAGGAAATCTGGGATT
CGGTCGTCGCCACTGTCCAGATGGCGCTGAGCGATGCCGGCGTTACGGCCAGGGATATTGCTGCACTTGGGATCACCAAC
CAGCGCGAGACGGTCGTCGTCTGGGAGCGCGAGACGGGCCGGCCGATCCAGAATGCCATCGTCTGGCAGGACCGGCGCAC
CGCAAGCTATTGCGACAATCTGAAACGGCAGGATCTCGAGCGGCTGTTCACCAAGAAGACCGGCCTGCTCCTCGATCCCT
ATTTCTCCGGGACGAAGCTTTCCTGGATGCTCGCCAACGTGAAGGGTGCCCGGGCACGCGCCGCCAGAGGCGATCTCTGC
TTCGGCACGATCGACACCTTCCTGATCTGGCGGCTGACGGGCGGCAAGAGCTTCGTCACCGACGCCACCAATGCCTCGCG
CACGCTGATGTACAATATCGCCGAAAACAGCTGGGACGAGGACCTGCTCGATATTCTGCGCGTGCCTGCCGCCATGCTGC
CTGAAGTGAAGGATTGCGCCGCCGAATTCGGCACGGTCGATTCCGAGATTTTCGGTGCTGCGATCCCGATCCTGGGCGTC
GCCGGCGACCAGCAGGCGGCGACCATCGGCCAGGCGTGTTTCCAGCCGGGCATGCTGAAATCGACCTACGGCACCGGCTG
TTTCGCGCTGCTCAATACCGGCGCCGACATGGTGCGTTCGAAAAACCGGCTGCTCACCACCATCGCCTATCGCCTGAACG
GCGAGACGACCTATGCGCTCGAGGGGTCGATCTTCATCGCCGGTGCCGCCGTGCAATGGCTGCGGGATGGGCTCGGCATC
ATCGGCAGCGCCGCCGAAACCAACGCGCTGGCCGAGAGGGCCGATCCGACGCAGGAGGTCTATCTCGTGCCCGCCTTCAC
CGGCCTCGGTGCACCGCATTGGGATGCCAAGGCACGCGGTGCGATCTTCGGGCTGACGCGCAATAGCGGCCCGGCGGAAC
TGTCCCGGGCGGCGCTCGAAGCCGTCTGCTACCAGACCCGTGATCTGCTCGACGCCATGCAGAAGGACTGGAAGAATGGC
GGCGACGACACGGTGCTGCGCGTCGACGGCGGCATGGTCGCCTCCGACTGGACGATGCAGCGCCTGGCCGATCTGCTCGA
TGCCCCGGTCGACCGGCCGGTGATCCTGGAGACGACGGCACTGGGTGCTGCCTGGCTCGCCGGCAGCCGGGCAGGCGTAT
GGCCCGACAGGGACGGTTTTTCGGCGGCATGGAAGCGGGACCGGCGGTTCGAGCCCGACATGGACGAGAAGGTGCGGGCG
GCCAAGCTCAAGGGCTGGAGAAGCGCTGTCAGGCGGACGCTGAGCGAGGGGTGA

Upstream 100 bases:

>100_bases
TTTCTGCGAATCACGGAAATGTTCTATCTCTTTTTCCACGCAATTCCGGAGGCAAAACCGCTTCGCACTTTTGCTGGAAT
TGCTTTAGCGGGAGGATTTC

Downstream 100 bases:

>100_bases
GGAAAGCGCTATAGTCTCTGGGCGTCTTCACGCAGCATCCAGTTATTTCTTCCGCGGAGCGTTGCCGTGCTCCGCAAGAT
CACGAATTCCATCCAGAATT

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MGGYVLAIDQGTTSTRAIVFDGDMHVAGKGQKEFTQIYPRSGWVEHDPEEIWDSVVATVQMALSDAGVTARDIAALGITN
QRETVVVWERETGRPIQNAIVWQDRRTASYCDNLKRQDLERLFTKKTGLLLDPYFSGTKLSWMLANVKGARARAARGDLC
FGTIDTFLIWRLTGGKSFVTDATNASRTLMYNIAENSWDEDLLDILRVPAAMLPEVKDCAAEFGTVDSEIFGAAIPILGV
AGDQQAATIGQACFQPGMLKSTYGTGCFALLNTGADMVRSKNRLLTTIAYRLNGETTYALEGSIFIAGAAVQWLRDGLGI
IGSAAETNALAERADPTQEVYLVPAFTGLGAPHWDAKARGAIFGLTRNSGPAELSRAALEAVCYQTRDLLDAMQKDWKNG
GDDTVLRVDGGMVASDWTMQRLADLLDAPVDRPVILETTALGAAWLAGSRAGVWPDRDGFSAAWKRDRRFEPDMDEKVRA
AKLKGWRSAVRRTLSEG

Sequences:

>Translated_497_residues
MGGYVLAIDQGTTSTRAIVFDGDMHVAGKGQKEFTQIYPRSGWVEHDPEEIWDSVVATVQMALSDAGVTARDIAALGITN
QRETVVVWERETGRPIQNAIVWQDRRTASYCDNLKRQDLERLFTKKTGLLLDPYFSGTKLSWMLANVKGARARAARGDLC
FGTIDTFLIWRLTGGKSFVTDATNASRTLMYNIAENSWDEDLLDILRVPAAMLPEVKDCAAEFGTVDSEIFGAAIPILGV
AGDQQAATIGQACFQPGMLKSTYGTGCFALLNTGADMVRSKNRLLTTIAYRLNGETTYALEGSIFIAGAAVQWLRDGLGI
IGSAAETNALAERADPTQEVYLVPAFTGLGAPHWDAKARGAIFGLTRNSGPAELSRAALEAVCYQTRDLLDAMQKDWKNG
GDDTVLRVDGGMVASDWTMQRLADLLDAPVDRPVILETTALGAAWLAGSRAGVWPDRDGFSAAWKRDRRFEPDMDEKVRA
AKLKGWRSAVRRTLSEG
>Mature_496_residues
GGYVLAIDQGTTSTRAIVFDGDMHVAGKGQKEFTQIYPRSGWVEHDPEEIWDSVVATVQMALSDAGVTARDIAALGITNQ
RETVVVWERETGRPIQNAIVWQDRRTASYCDNLKRQDLERLFTKKTGLLLDPYFSGTKLSWMLANVKGARARAARGDLCF
GTIDTFLIWRLTGGKSFVTDATNASRTLMYNIAENSWDEDLLDILRVPAAMLPEVKDCAAEFGTVDSEIFGAAIPILGVA
GDQQAATIGQACFQPGMLKSTYGTGCFALLNTGADMVRSKNRLLTTIAYRLNGETTYALEGSIFIAGAAVQWLRDGLGII
GSAAETNALAERADPTQEVYLVPAFTGLGAPHWDAKARGAIFGLTRNSGPAELSRAALEAVCYQTRDLLDAMQKDWKNGG
DDTVLRVDGGMVASDWTMQRLADLLDAPVDRPVILETTALGAAWLAGSRAGVWPDRDGFSAAWKRDRRFEPDMDEKVRAA
KLKGWRSAVRRTLSEG

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=508, Percent_Identity=43.1102362204724, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4504007, Length=508, Percent_Identity=43.1102362204724, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI42794763, Length=513, Percent_Identity=42.8849902534113, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI41393575, Length=507, Percent_Identity=41.6173570019724, Blast_Score=376, Evalue=1e-104,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=31.8271119842829, Blast_Score=301, Evalue=1e-81,
Organism=Escherichia coli, GI1790361, Length=493, Percent_Identity=51.7241379310345, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1789987, Length=434, Percent_Identity=25.8064516129032, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787789, Length=522, Percent_Identity=24.7126436781609, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790005, Length=489, Percent_Identity=23.5173824130879, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=496, Percent_Identity=40.1209677419355, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71986140, Length=518, Percent_Identity=24.5173745173745, Blast_Score=175, Evalue=5e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=298, Percent_Identity=25.8389261744966, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321755, Length=508, Percent_Identity=35.4330708661417, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=40.8203125, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24655555, Length=508, Percent_Identity=37.7952755905512, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24655560, Length=508, Percent_Identity=37.7952755905512, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24655570, Length=508, Percent_Identity=37.7952755905512, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24655574, Length=508, Percent_Identity=37.7952755905512, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24655565, Length=508, Percent_Identity=37.7952755905512, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24654573, Length=447, Percent_Identity=41.3870246085011, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=31.015037593985, Blast_Score=246, Evalue=3e-65,
Organism=Drosophila melanogaster, GI24652871, Length=492, Percent_Identity=31.9105691056911, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=29.0448343079922, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=28.4905660377358, Blast_Score=201, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24656372, Length=347, Percent_Identity=29.6829971181556, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45552909, Length=250, Percent_Identity=29.2, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54161; Mature: 54030

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGYVLAIDQGTTSTRAIVFDGDMHVAGKGQKEFTQIYPRSGWVEHDPEEIWDSVVATVQ
CCCEEEEEECCCCCCEEEEEECCEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MALSDAGVTARDIAALGITNQRETVVVWERETGRPIQNAIVWQDRRTASYCDNLKRQDLE
HHHHCCCCCHHHHHEECCCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHHHHH
RLFTKKTGLLLDPYFSGTKLSWMLANVKGARARAARGDLCFGTIDTFLIWRLTGGKSFVT
HHHHHCCCCEECCCCCCCEEEEEEECCCCCHHHCCCCCEEEECCCEEEEEEECCCCEEEE
DATNASRTLMYNIAENSWDEDLLDILRVPAAMLPEVKDCAAEFGTVDSEIFGAAIPILGV
CCCCCCEEEEEEECCCCCCHHHHHHHHCCHHHCCCHHHHHHHHCCCCHHHHHHHHCEEEE
AGDQQAATIGQACFQPGMLKSTYGTGCFALLNTGADMVRSKNRLLTTIAYRLNGETTYAL
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCEEEEEEEEECCCEEEEE
EGSIFIAGAAVQWLRDGLGIIGSAAETNALAERADPTQEVYLVPAFTGLGAPHWDAKARG
ECCEEEEHHHHHHHHHCHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCC
AIFGLTRNSGPAELSRAALEAVCYQTRDLLDAMQKDWKNGGDDTVLRVDGGMVASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCHHHH
RLADLLDAPVDRPVILETTALGAAWLAGSRAGVWPDRDGFSAAWKRDRRFEPDMDEKVRA
HHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHH
AKLKGWRSAVRRTLSEG
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GGYVLAIDQGTTSTRAIVFDGDMHVAGKGQKEFTQIYPRSGWVEHDPEEIWDSVVATVQ
CCEEEEEECCCCCCEEEEEECCEEECCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MALSDAGVTARDIAALGITNQRETVVVWERETGRPIQNAIVWQDRRTASYCDNLKRQDLE
HHHHCCCCCHHHHHEECCCCCCCEEEEEECCCCCCHHHHEEECCCCHHHHHHHHHHHHHH
RLFTKKTGLLLDPYFSGTKLSWMLANVKGARARAARGDLCFGTIDTFLIWRLTGGKSFVT
HHHHHCCCCEECCCCCCCEEEEEEECCCCCHHHCCCCCEEEECCCEEEEEEECCCCEEEE
DATNASRTLMYNIAENSWDEDLLDILRVPAAMLPEVKDCAAEFGTVDSEIFGAAIPILGV
CCCCCCEEEEEEECCCCCCHHHHHHHHCCHHHCCCHHHHHHHHCCCCHHHHHHHHCEEEE
AGDQQAATIGQACFQPGMLKSTYGTGCFALLNTGADMVRSKNRLLTTIAYRLNGETTYAL
CCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCHHHHHCCCCEEEEEEEEECCCEEEEE
EGSIFIAGAAVQWLRDGLGIIGSAAETNALAERADPTQEVYLVPAFTGLGAPHWDAKARG
ECCEEEEHHHHHHHHHCHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCC
AIFGLTRNSGPAELSRAALEAVCYQTRDLLDAMQKDWKNGGDDTVLRVDGGMVASDWTMQ
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCHHHH
RLADLLDAPVDRPVILETTALGAAWLAGSRAGVWPDRDGFSAAWKRDRRFEPDMDEKVRA
HHHHHHCCCCCCCEEEEEHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCCCCCHHHHHHH
AKLKGWRSAVRRTLSEG
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA