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The map label for this gene is aglA [H]

Identifier: 209550412

GI number: 209550412

Start: 2882260

End: 2883906

Strand: Reverse

Name: aglA [H]

Synonym: Rleg2_2833

Alternate gene names: 209550412

Gene position: 2883906-2882260 (Counterclockwise)

Preceding gene: 209550420

Following gene: 209550408

Centisome position: 63.55

GC content: 61.38

Gene sequence:

>1647_bases
ATGGCATTGAAGGCAGGCGGGAATGCGGACTGGTGGCGCGGCGCGGTGATCTACCAGGTCTATCCGCGCTCGTTTCAGGA
CACCAACAGCGACGGGCTTGGCGACCTCAAGGGGATTACCCGCCGCCTGCCGCATATCGCTAGTCTCGGTGTCGACGCGA
TCTGGCTTTCGCCCTTCTTCAAGTCGCCGATGGCCGACATGGGGTATGATGTTTCCGATTATTGCGACGTCGACCCGATC
TTCGGCACGCTCGCCGATTTCGACGAGATGATGGCGGAGGCGCACAGGCTCGGCATCAAGGTCGTCATCGACCAGGTGAT
CTCGCACACATCGGACCGGCACCCCTGGTTCGTCGAGAGCCGGACGAGCCGGACCAATGCGAAGGCGGACTGGTATGTCT
GGGCCGATCCGAAGCCGGATGGCACGGCGCCGAACAATTGGCTGTCGATTTTCGGCGGGCCGGGCTGGGAGTGGGACGGC
GTGCGCCGGCAATATTACCAGCACAATTTCCTGACCTCGCAGCCGGACCTGAATTTCCACAGCAGGGAAGTGCAGGATGC
GGTGCTGGAGACGGTGAAATTCTGGCTCGACCGCGGCGTCGACGGCTTCCGGCTGGATACCGTCAACTATTATTTCTGCG
ACAAGCAGCTCAGAAGCAATCCGCCGCACGAGCCCGATGAGGATGATGCAGGCCTCGATGCGCCCGACAGCAATCCCTAT
GGCATGCAGAATCATCTCTATGACAAGACGCAGCCGGAAAACGTCGATTTCCTCAAACGCTTCCGGGTGCTGCTCGACCA
ATATCAGGATCGTACCACCGTCGGCGAGGTCGGCGACGGCGCGCGTTCACTGAAGACGGTGGCGGCCTATACGAGCGGCG
GCGACAAGCTGCACATGTGCTACACCTTCGACCTGCTGGGACCGGATTTTACCGCCGAGCATATTCGCGGCTGCGTCGAA
GCTTTCCAAAAATCCGTGACCGATGGCTGGGTCTGTTGGGCTTTTTCAAACCACGACGTCATGCGCCATGTCAGCCGTTT
TGCGGTGACGGAGGAAGAGCGGCCCGTCATCGCCAAACTGGCGATCTCGGTGCTGTCGGCGCTGCGCGGTTCGATCTGCC
TCTATCAGGGCGAGGAGCTCGGGCTGCCGGAGGCGGAGCTTGCCTTCGAAGATCTGCGCGACCCTTACGGCATCCGCTTC
TGGCCGGCCTTCAAAGGCCGCGACGGATGCCGCACGCCGATGCCCTGGGAGGCGGGCAAGGCACATGCGGGCTTTACTTC
AGCCGAAAAGAGCTGGCTGCCGGTGCCTTACGAGCAGGCGGCACTTTCCGTGGATACGCAGGAGGGAAGCGACAGCTCGG
TGCTGCATCACTATCGCAAGACGCTCGCATTCCGGAAGAGCCATGCGGCGCTAGTCGACGGCGAAATGACCTTCATCGCC
ACCAATCAGGATCTGCTCGTCTTCACCCGGGAAAAGGACGGGGGAAAACTGCTCTTCGTTTTCAACCTGACGCGCAAACC
GGCGGAATTCCGTCTGCCTGAAGGCATCGTGTTGGGCGAACCGCTCGAGATGCCCGGGTTCGAGGCGGTGTCGGTTTCGG
GGCTGGTGAAGCTTGCGGCGCTGGATGGGTTCTGTGCGCGGATTTGA

Upstream 100 bases:

>100_bases
ACTTTGCGACATTCCAGCACCTTCATGACAGTCTCACATGTTGTCTTTTGTTTCGTCCGCTTTAGTGTGGCGAGCGCTAA
CGAGGCAGGAGATATGGCCG

Downstream 100 bases:

>100_bases
TGTCTATTTTAGAAGCTTATCGTATTCGGCATGAGAGCCGATCCAGAACCAGACGATATCGTCGCCGTCCCTGATGGCCA
AAGCTCTCCAGGAGGTGCCG

Product: alpha amylase catalytic protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MALKAGGNADWWRGAVIYQVYPRSFQDTNSDGLGDLKGITRRLPHIASLGVDAIWLSPFFKSPMADMGYDVSDYCDVDPI
FGTLADFDEMMAEAHRLGIKVVIDQVISHTSDRHPWFVESRTSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDG
VRRQYYQHNFLTSQPDLNFHSREVQDAVLETVKFWLDRGVDGFRLDTVNYYFCDKQLRSNPPHEPDEDDAGLDAPDSNPY
GMQNHLYDKTQPENVDFLKRFRVLLDQYQDRTTVGEVGDGARSLKTVAAYTSGGDKLHMCYTFDLLGPDFTAEHIRGCVE
AFQKSVTDGWVCWAFSNHDVMRHVSRFAVTEEERPVIAKLAISVLSALRGSICLYQGEELGLPEAELAFEDLRDPYGIRF
WPAFKGRDGCRTPMPWEAGKAHAGFTSAEKSWLPVPYEQAALSVDTQEGSDSSVLHHYRKTLAFRKSHAALVDGEMTFIA
TNQDLLVFTREKDGGKLLFVFNLTRKPAEFRLPEGIVLGEPLEMPGFEAVSVSGLVKLAALDGFCARI

Sequences:

>Translated_548_residues
MALKAGGNADWWRGAVIYQVYPRSFQDTNSDGLGDLKGITRRLPHIASLGVDAIWLSPFFKSPMADMGYDVSDYCDVDPI
FGTLADFDEMMAEAHRLGIKVVIDQVISHTSDRHPWFVESRTSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDG
VRRQYYQHNFLTSQPDLNFHSREVQDAVLETVKFWLDRGVDGFRLDTVNYYFCDKQLRSNPPHEPDEDDAGLDAPDSNPY
GMQNHLYDKTQPENVDFLKRFRVLLDQYQDRTTVGEVGDGARSLKTVAAYTSGGDKLHMCYTFDLLGPDFTAEHIRGCVE
AFQKSVTDGWVCWAFSNHDVMRHVSRFAVTEEERPVIAKLAISVLSALRGSICLYQGEELGLPEAELAFEDLRDPYGIRF
WPAFKGRDGCRTPMPWEAGKAHAGFTSAEKSWLPVPYEQAALSVDTQEGSDSSVLHHYRKTLAFRKSHAALVDGEMTFIA
TNQDLLVFTREKDGGKLLFVFNLTRKPAEFRLPEGIVLGEPLEMPGFEAVSVSGLVKLAALDGFCARI
>Mature_547_residues
ALKAGGNADWWRGAVIYQVYPRSFQDTNSDGLGDLKGITRRLPHIASLGVDAIWLSPFFKSPMADMGYDVSDYCDVDPIF
GTLADFDEMMAEAHRLGIKVVIDQVISHTSDRHPWFVESRTSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGV
RRQYYQHNFLTSQPDLNFHSREVQDAVLETVKFWLDRGVDGFRLDTVNYYFCDKQLRSNPPHEPDEDDAGLDAPDSNPYG
MQNHLYDKTQPENVDFLKRFRVLLDQYQDRTTVGEVGDGARSLKTVAAYTSGGDKLHMCYTFDLLGPDFTAEHIRGCVEA
FQKSVTDGWVCWAFSNHDVMRHVSRFAVTEEERPVIAKLAISVLSALRGSICLYQGEELGLPEAELAFEDLRDPYGIRFW
PAFKGRDGCRTPMPWEAGKAHAGFTSAEKSWLPVPYEQAALSVDTQEGSDSSVLHHYRKTLAFRKSHAALVDGEMTFIAT
NQDLLVFTREKDGGKLLFVFNLTRKPAEFRLPEGIVLGEPLEMPGFEAVSVSGLVKLAALDGFCARI

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=534, Percent_Identity=31.6479400749064, Blast_Score=272, Evalue=7e-73,
Organism=Escherichia coli, GI1790687, Length=529, Percent_Identity=34.9716446124764, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1786604, Length=495, Percent_Identity=23.2323232323232, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32565753, Length=494, Percent_Identity=25.9109311740891, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147709, Length=538, Percent_Identity=24.3494423791822, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322245, Length=560, Percent_Identity=33.3928571428571, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321731, Length=557, Percent_Identity=35.3680430879713, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6319776, Length=557, Percent_Identity=35.3680430879713, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6321726, Length=548, Percent_Identity=32.4817518248175, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6324416, Length=550, Percent_Identity=32.7272727272727, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322241, Length=550, Percent_Identity=32.3636363636364, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322021, Length=550, Percent_Identity=32.3636363636364, Blast_Score=262, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24586587, Length=504, Percent_Identity=36.5079365079365, Blast_Score=323, Evalue=2e-88,
Organism=Drosophila melanogaster, GI24583745, Length=546, Percent_Identity=35.1648351648352, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI45549022, Length=542, Percent_Identity=33.7638376383764, Blast_Score=306, Evalue=3e-83,
Organism=Drosophila melanogaster, GI24586599, Length=519, Percent_Identity=33.9113680154143, Blast_Score=301, Evalue=1e-81,
Organism=Drosophila melanogaster, GI24583749, Length=547, Percent_Identity=35.6489945155393, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24583747, Length=547, Percent_Identity=35.6489945155393, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24586597, Length=536, Percent_Identity=34.5149253731343, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24586593, Length=520, Percent_Identity=33.4615384615385, Blast_Score=286, Evalue=3e-77,
Organism=Drosophila melanogaster, GI24586591, Length=513, Percent_Identity=34.1130604288499, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI221330053, Length=484, Percent_Identity=35.1239669421488, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24586589, Length=505, Percent_Identity=34.0594059405941, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI281360393, Length=460, Percent_Identity=30.8695652173913, Blast_Score=210, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61653; Mature: 61522

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKAGGNADWWRGAVIYQVYPRSFQDTNSDGLGDLKGITRRLPHIASLGVDAIWLSPFF
CCCCCCCCCCHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCCEECCHHH
KSPMADMGYDVSDYCDVDPIFGTLADFDEMMAEAHRLGIKVVIDQVISHTSDRHPWFVES
CCCHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEC
RTSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGVRRQYYQHNFLTSQPDLNFH
CCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCC
SREVQDAVLETVKFWLDRGVDGFRLDTVNYYFCDKQLRSNPPHEPDEDDAGLDAPDSNPY
HHHHHHHHHHHHHHHHHCCCCCEEEECEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCC
GMQNHLYDKTQPENVDFLKRFRVLLDQYQDRTTVGEVGDGARSLKTVAAYTSGGDKLHMC
CCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEEECCCCEEEEE
YTFDLLGPDFTAEHIRGCVEAFQKSVTDGWVCWAFSNHDVMRHVSRFAVTEEERPVIAKL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHH
AISVLSALRGSICLYQGEELGLPEAELAFEDLRDPYGIRFWPAFKGRDGCRTPMPWEAGK
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCC
AHAGFTSAEKSWLPVPYEQAALSVDTQEGSDSSVLHHYRKTLAFRKSHAALVDGEMTFIA
CCCCCCCCCCCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEEE
TNQDLLVFTREKDGGKLLFVFNLTRKPAEFRLPEGIVLGEPLEMPGFEAVSVSGLVKLAA
ECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEHHHHHHHHH
LDGFCARI
HCHHHHCC
>Mature Secondary Structure 
ALKAGGNADWWRGAVIYQVYPRSFQDTNSDGLGDLKGITRRLPHIASLGVDAIWLSPFF
CCCCCCCCCHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCCEECCHHH
KSPMADMGYDVSDYCDVDPIFGTLADFDEMMAEAHRLGIKVVIDQVISHTSDRHPWFVES
CCCHHHCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEC
RTSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGVRRQYYQHNFLTSQPDLNFH
CCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCC
SREVQDAVLETVKFWLDRGVDGFRLDTVNYYFCDKQLRSNPPHEPDEDDAGLDAPDSNPY
HHHHHHHHHHHHHHHHHCCCCCEEEECEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCC
GMQNHLYDKTQPENVDFLKRFRVLLDQYQDRTTVGEVGDGARSLKTVAAYTSGGDKLHMC
CCHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHEEEECCCCEEEEE
YTFDLLGPDFTAEHIRGCVEAFQKSVTDGWVCWAFSNHDVMRHVSRFAVTEEERPVIAKL
EEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHHHHH
AISVLSALRGSICLYQGEELGLPEAELAFEDLRDPYGIRFWPAFKGRDGCRTPMPWEAGK
HHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCC
AHAGFTSAEKSWLPVPYEQAALSVDTQEGSDSSVLHHYRKTLAFRKSHAALVDGEMTFIA
CCCCCCCCCCCCCCCCHHHHEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCEEEEE
TNQDLLVFTREKDGGKLLFVFNLTRKPAEFRLPEGIVLGEPLEMPGFEAVSVSGLVKLAA
ECCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCEEEHHHHHHHHH
LDGFCARI
HCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]