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The map label for this gene is cca [H]

Identifier: 209550368

GI number: 209550368

Start: 2832539

End: 2833807

Strand: Reverse

Name: cca [H]

Synonym: Rleg2_2789

Alternate gene names: 209550368

Gene position: 2833807-2832539 (Counterclockwise)

Preceding gene: 209550369

Following gene: 209550364

Centisome position: 62.45

GC content: 65.09

Gene sequence:

>1269_bases
ATGACCGGTCTTGCCGACCAAGCATGGTTCCGCGACCCGGCGCTCGGCCGGATCCTGGAACTTCTGAATGCCGATGGCGG
GGCCGATGGTGGCGGGGAGGCGCGGGTGGTCGGCGGCGCGGTGCGCAACAGCCTGATGGACCTGCCGGTCAGCGATATCG
ACATCGCTACCACGCTCAGGCCGGAGGCGGTGATGGAGAAGGCGGCTGCGGCCGGCATCAAGGCGGTTCCGACGGGCCTG
CAGCACGGCACGGTGACGCTTGTTGTCGATGGCAAGCCTTTCGAAGTGACGACGCTTCGCACCGATGTCGAGACCGACGG
CCGCCATGCCAAGGTTGCCTTCAGCACTGATTGGAAGGCGGATGCCGAGCGGCGGGATCTGACCATGAATGCGCTTTATG
CGGATGCCAATGGCGAGGTGGTTGATCTGGTCGGCGGGCTTGCCGATATCGAGAGGCGCAACATCCGCTTCATCGGTGAT
GCGGCCAAGCGTATTGCCGAAGATCATTTGCGCATCCTGCGCTTTTTCCGCTTCTTTGCCTATTACGGCTCCGGACGGCC
GGATGCCGAGGGGCTGAAGGCGTGTTCGGCGGCACGTTCGAAATTGAAGACGCTGTCGGCGGAGCGTGTCTGGTCGGAGC
TGCGCAAGCTGCTGAGTGCTGCCGATCCCGGCCGGGCGCTGCTCTGGATGCGGCAGGTGGCGGTGCTGACGGAAATCCTG
CCGGAATCGGAACGATGGGGGATCGATGCGATCCCTTCGCTGGTTGCCACCGAAAAGGCGCTCGGCTGGGCGCCCGATCC
GCTGCTGCGGCTTGCGGCGATCGTGCCGCCCGATGCGGCGCGGCTGGAGGCGCTGGCAGCCCGACTCAAGCTTGCGAACG
CGGAAGCGGCTTTCCTCAAGGCCTGGGCAATGGCAGCCCCCGTCAATGACGACATGTCGTCGGCCGCCTTCGAACGGCTG
CTTTACCGCAACGGCGCCGATGGCATCACGACGCGGCTGAAGCTGGCGCTCGGCGTTGCGCGTGGCAAAGCTGAAGGGGA
TTTCGACGAAATGGCGCGGTCGGCCCGGCTTACCAAGCTCCTGGATCAGGCCGCGAACTGGGAGAAACCGCAATTTCCGC
TGAACGGCGGCGACGTGATGTCTATGGGAATTCCGTCCGGCCCGCGTGTCGGCGAGCTGCTAGCCGGCCTGGAAAACCAG
TGGGTGGAGGAAAATTTCGTTTCCGACCGGGCGGCGCTGCTCGCCCGGCTGCAGGAACGGGTGCAATGA

Upstream 100 bases:

>100_bases
ACGGCATCGACCGGCATTTCTACCGGATGCCCTATGAAACCAGAATGATCTGGGGCATCACCGCCGGCATCGTCCGCACG
CTTTACGAAAGGCTTTATGC

Downstream 100 bases:

>100_bases
TCAGGCTGCGTTGACCTCGTCTCGGATGCGAGACTTGATATTATCCACCATGTTCTCGCGAATCGTGGTTTCGCCGTGAG
CGTTTTTCAGGTGCTCGACG

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTGLADQAWFRDPALGRILELLNADGGADGGGEARVVGGAVRNSLMDLPVSDIDIATTLRPEAVMEKAAAAGIKAVPTGL
QHGTVTLVVDGKPFEVTTLRTDVETDGRHAKVAFSTDWKADAERRDLTMNALYADANGEVVDLVGGLADIERRNIRFIGD
AAKRIAEDHLRILRFFRFFAYYGSGRPDAEGLKACSAARSKLKTLSAERVWSELRKLLSAADPGRALLWMRQVAVLTEIL
PESERWGIDAIPSLVATEKALGWAPDPLLRLAAIVPPDAARLEALAARLKLANAEAAFLKAWAMAAPVNDDMSSAAFERL
LYRNGADGITTRLKLALGVARGKAEGDFDEMARSARLTKLLDQAANWEKPQFPLNGGDVMSMGIPSGPRVGELLAGLENQ
WVEENFVSDRAALLARLQERVQ

Sequences:

>Translated_422_residues
MTGLADQAWFRDPALGRILELLNADGGADGGGEARVVGGAVRNSLMDLPVSDIDIATTLRPEAVMEKAAAAGIKAVPTGL
QHGTVTLVVDGKPFEVTTLRTDVETDGRHAKVAFSTDWKADAERRDLTMNALYADANGEVVDLVGGLADIERRNIRFIGD
AAKRIAEDHLRILRFFRFFAYYGSGRPDAEGLKACSAARSKLKTLSAERVWSELRKLLSAADPGRALLWMRQVAVLTEIL
PESERWGIDAIPSLVATEKALGWAPDPLLRLAAIVPPDAARLEALAARLKLANAEAAFLKAWAMAAPVNDDMSSAAFERL
LYRNGADGITTRLKLALGVARGKAEGDFDEMARSARLTKLLDQAANWEKPQFPLNGGDVMSMGIPSGPRVGELLAGLENQ
WVEENFVSDRAALLARLQERVQ
>Mature_421_residues
TGLADQAWFRDPALGRILELLNADGGADGGGEARVVGGAVRNSLMDLPVSDIDIATTLRPEAVMEKAAAAGIKAVPTGLQ
HGTVTLVVDGKPFEVTTLRTDVETDGRHAKVAFSTDWKADAERRDLTMNALYADANGEVVDLVGGLADIERRNIRFIGDA
AKRIAEDHLRILRFFRFFAYYGSGRPDAEGLKACSAARSKLKTLSAERVWSELRKLLSAADPGRALLWMRQVAVLTEILP
ESERWGIDAIPSLVATEKALGWAPDPLLRLAAIVPPDAARLEALAARLKLANAEAAFLKAWAMAAPVNDDMSSAAFERLL
YRNGADGITTRLKLALGVARGKAEGDFDEMARSARLTKLLDQAANWEKPQFPLNGGDVMSMGIPSGPRVGELLAGLENQW
VEENFVSDRAALLARLQERVQ

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=397, Percent_Identity=33.5012594458438, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1789436, Length=240, Percent_Identity=30.4166666666667, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87081691, Length=230, Percent_Identity=28.695652173913, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71995920, Length=420, Percent_Identity=32.8571428571429, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI281360112, Length=378, Percent_Identity=31.7460317460317, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI21357337, Length=378, Percent_Identity=31.7460317460317, Blast_Score=179, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 45603; Mature: 45471

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGLADQAWFRDPALGRILELLNADGGADGGGEARVVGGAVRNSLMDLPVSDIDIATTLR
CCCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCHHHCCC
PEAVMEKAAAAGIKAVPTGLQHGTVTLVVDGKPFEVTTLRTDVETDGRHAKVAFSTDWKA
HHHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECCCCC
DAERRDLTMNALYADANGEVVDLVGGLADIERRNIRFIGDAAKRIAEDHLRILRFFRFFA
CCHHHCCEEEEEEECCCCCCHHHHHCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
YYGSGRPDAEGLKACSAARSKLKTLSAERVWSELRKLLSAADPGRALLWMRQVAVLTEIL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
PESERWGIDAIPSLVATEKALGWAPDPLLRLAAIVPPDAARLEALAARLKLANAEAAFLK
CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHH
AWAMAAPVNDDMSSAAFERLLYRNGADGITTRLKLALGVARGKAEGDFDEMARSARLTKL
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LDQAANWEKPQFPLNGGDVMSMGIPSGPRVGELLAGLENQWVEENFVSDRAALLARLQER
HHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQ
CC
>Mature Secondary Structure 
TGLADQAWFRDPALGRILELLNADGGADGGGEARVVGGAVRNSLMDLPVSDIDIATTLR
CCCCCHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECHHHHHHHHCCCCCCCCHHHCCC
PEAVMEKAAAAGIKAVPTGLQHGTVTLVVDGKPFEVTTLRTDVETDGRHAKVAFSTDWKA
HHHHHHHHHHCCCHHCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCEEEEEEECCCCC
DAERRDLTMNALYADANGEVVDLVGGLADIERRNIRFIGDAAKRIAEDHLRILRFFRFFA
CCHHHCCEEEEEEECCCCCCHHHHHCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHH
YYGSGRPDAEGLKACSAARSKLKTLSAERVWSELRKLLSAADPGRALLWMRQVAVLTEIL
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHC
PESERWGIDAIPSLVATEKALGWAPDPLLRLAAIVPPDAARLEALAARLKLANAEAAFLK
CCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHH
AWAMAAPVNDDMSSAAFERLLYRNGADGITTRLKLALGVARGKAEGDFDEMARSARLTKL
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LDQAANWEKPQFPLNGGDVMSMGIPSGPRVGELLAGLENQWVEENFVSDRAALLARLQER
HHHHCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11442348 [H]