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The map label for this gene is norM [H]

Identifier: 209550288

GI number: 209550288

Start: 2748707

End: 2750110

Strand: Reverse

Name: norM [H]

Synonym: Rleg2_2708

Alternate gene names: 209550288

Gene position: 2750110-2748707 (Counterclockwise)

Preceding gene: 209550289

Following gene: 209550285

Centisome position: 60.6

GC content: 64.17

Gene sequence:

>1404_bases
ATGGACACGCCGATCGACGCGCGCAGGCTTGCGCCGACAACCGATAATCGTTGGGGCGCACATTTCCGTGCAACGCTGGC
GCTCGGCATTCCGCTGATCGGCGCGCAGCTCGCCCAGCTCGGCATCAACACCACCGATGTGATGATCGTCGGCCGCCTCG
GCGCCGAGCATCTGGCGGCAATGGTGCTGTCCGCCCAGTTCCTGTTTACGATCCTGATCTTCGGTTCCGGCTTTGCCATT
GCGGTCATCCCGATGGTGGCGCAGGCCTATGGCCGCGGCGACGTCGTCTCGGTGCGCCGGGCGCTGCGTATGGGTCTCTG
GGTGGTGATCGCATATTGGGTCATCATGCAGCCCGCCTTCTTCAATTCGGAGCAGATCCTCCTTTTCGCGCAGCAGAAGC
CCGAGGTGGCCAAGCTCGCCCATGGCTACATCATGATCGGCCAGTTCGGCGTGCTGCCGGCGCTGCTTTTCAATGTCATG
CGCGCGCTGGTCAGCGCCATCGGCAAGGCGGGCATCATCCTCAACGTCACCATCGCCACGCTGGTGCTGAACGCGATCTT
CGCCTATGCCCTGGTGCTCGGCCATTTCGGCTTTCCGGCGATGGGGCTCGAGGGTGCTGCGATCGTGTCTGTCGTCGTGC
AGACGGCCGGTTTTCTCTTCATCCTCGCCTTCGTCCAGAAGCGCGAGGAGACACGGCGCTACGAGATCTTCGTGCGGTTC
TGGAAGCCCGACTGGCATGCGCTGCTGGAGGTCCTCCGGCTCGGTTTTCCGATCAGCGTCACCGTGCTTGCCGAGGTAAG
TCTGTTCACGGTGGCCTCGCTGCTGATGGGCTATATCGGCACCATCGAACTCGCCGCTCACGGTATCGCCCTGCAATGGG
CTTCGATCGCCTTCATGATCCCGCTCGGCCTTAGCCAGGCGGCGACGGTGCGCGTCGGTGTCGCCCACGGGCAGGGCGAT
TACCGCGGGCTGGTGCGGGCTTCGATCATGGTGCTCATCCTTGCCTGCGCCATTTCGGCGGTGGGCTCGGTGCTGTTTGC
CACCATGCCGCAATTCCTCGGCAGCTGGTTCCTCGACGTCAATTCGCCGGAGGCGCCCGCCGTGCTGGCTTATGCCGGGC
CGCTGATCGTCGTCGCCGGGCTTTTCCAGCTGGTCGACGGATTGCAGGTGATCGCCAACGGATTGCTGCGCGGTCTGAAA
GACGCGCGGGTGCCTATGATCATGGCACTGATTGCCTATTGGCCGATCGGCTTCTTCCTCGCCTGGGCCTTTGCCTTCCC
GCTGGGCTTCGGCGGCATCGGCATCTGGTTCGGCTTCCTCGTCGGGCTGGCGGCTGCCGCCGTCATGCTCTGCTGGCGGT
TCTATCTTCTGCTTCGCCGGGAAGGAGCGATGGCCGGCGCCTGA

Upstream 100 bases:

>100_bases
CTGTTTAGGGTGGTTCTTTGCTGGGGTTTGATCTAAATCCCGATGATATTTCTCCGCAGATTGCGCGGCGACCCATATTT
CAATCACAGGACTAGGTGTC

Downstream 100 bases:

>100_bases
GCCGGCCATCCTTATCCTCAAGCCGCGACAGGCTTGTAGCTCGCCATGTCGGCGCGAATGCCGAGACGCGCAAACATTTC
CTGCGGGTCGAAATTCGCGG

Product: MATE efflux family protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MDTPIDARRLAPTTDNRWGAHFRATLALGIPLIGAQLAQLGINTTDVMIVGRLGAEHLAAMVLSAQFLFTILIFGSGFAI
AVIPMVAQAYGRGDVVSVRRALRMGLWVVIAYWVIMQPAFFNSEQILLFAQQKPEVAKLAHGYIMIGQFGVLPALLFNVM
RALVSAIGKAGIILNVTIATLVLNAIFAYALVLGHFGFPAMGLEGAAIVSVVVQTAGFLFILAFVQKREETRRYEIFVRF
WKPDWHALLEVLRLGFPISVTVLAEVSLFTVASLLMGYIGTIELAAHGIALQWASIAFMIPLGLSQAATVRVGVAHGQGD
YRGLVRASIMVLILACAISAVGSVLFATMPQFLGSWFLDVNSPEAPAVLAYAGPLIVVAGLFQLVDGLQVIANGLLRGLK
DARVPMIMALIAYWPIGFFLAWAFAFPLGFGGIGIWFGFLVGLAAAAVMLCWRFYLLLRREGAMAGA

Sequences:

>Translated_467_residues
MDTPIDARRLAPTTDNRWGAHFRATLALGIPLIGAQLAQLGINTTDVMIVGRLGAEHLAAMVLSAQFLFTILIFGSGFAI
AVIPMVAQAYGRGDVVSVRRALRMGLWVVIAYWVIMQPAFFNSEQILLFAQQKPEVAKLAHGYIMIGQFGVLPALLFNVM
RALVSAIGKAGIILNVTIATLVLNAIFAYALVLGHFGFPAMGLEGAAIVSVVVQTAGFLFILAFVQKREETRRYEIFVRF
WKPDWHALLEVLRLGFPISVTVLAEVSLFTVASLLMGYIGTIELAAHGIALQWASIAFMIPLGLSQAATVRVGVAHGQGD
YRGLVRASIMVLILACAISAVGSVLFATMPQFLGSWFLDVNSPEAPAVLAYAGPLIVVAGLFQLVDGLQVIANGLLRGLK
DARVPMIMALIAYWPIGFFLAWAFAFPLGFGGIGIWFGFLVGLAAAAVMLCWRFYLLLRREGAMAGA
>Mature_467_residues
MDTPIDARRLAPTTDNRWGAHFRATLALGIPLIGAQLAQLGINTTDVMIVGRLGAEHLAAMVLSAQFLFTILIFGSGFAI
AVIPMVAQAYGRGDVVSVRRALRMGLWVVIAYWVIMQPAFFNSEQILLFAQQKPEVAKLAHGYIMIGQFGVLPALLFNVM
RALVSAIGKAGIILNVTIATLVLNAIFAYALVLGHFGFPAMGLEGAAIVSVVVQTAGFLFILAFVQKREETRRYEIFVRF
WKPDWHALLEVLRLGFPISVTVLAEVSLFTVASLLMGYIGTIELAAHGIALQWASIAFMIPLGLSQAATVRVGVAHGQGD
YRGLVRASIMVLILACAISAVGSVLFATMPQFLGSWFLDVNSPEAPAVLAYAGPLIVVAGLFQLVDGLQVIANGLLRGLK
DARVPMIMALIAYWPIGFFLAWAFAFPLGFGGIGIWFGFLVGLAAAAVMLCWRFYLLLRREGAMAGA

Specific function: Multidrug efflux pump [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=434, Percent_Identity=27.1889400921659, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI153792564, Length=444, Percent_Identity=25.9009009009009, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI38261960, Length=479, Percent_Identity=24.4258872651357, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=29.1028446389497, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI226510954, Length=443, Percent_Identity=23.2505643340858, Blast_Score=66, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6321821, Length=387, Percent_Identity=25.5813953488372, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6320545, Length=425, Percent_Identity=24.4705882352941, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 50283; Mature: 50283

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPIDARRLAPTTDNRWGAHFRATLALGIPLIGAQLAQLGINTTDVMIVGRLGAEHLAA
CCCCCCCHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
MVLSAQFLFTILIFGSGFAIAVIPMVAQAYGRGDVVSVRRALRMGLWVVIAYWVIMQPAF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
FNSEQILLFAQQKPEVAKLAHGYIMIGQFGVLPALLFNVMRALVSAIGKAGIILNVTIAT
CCCCEEEEEECCCCHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
LVLNAIFAYALVLGHFGFPAMGLEGAAIVSVVVQTAGFLFILAFVQKREETRRYEIFVRF
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
WKPDWHALLEVLRLGFPISVTVLAEVSLFTVASLLMGYIGTIELAAHGIALQWASIAFMI
ECCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
PLGLSQAATVRVGVAHGQGDYRGLVRASIMVLILACAISAVGSVLFATMPQFLGSWFLDV
HCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
NSPEAPAVLAYAGPLIVVAGLFQLVDGLQVIANGLLRGLKDARVPMIMALIAYWPIGFFL
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWAFAFPLGFGGIGIWFGFLVGLAAAAVMLCWRFYLLLRREGAMAGA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MDTPIDARRLAPTTDNRWGAHFRATLALGIPLIGAQLAQLGINTTDVMIVGRLGAEHLAA
CCCCCCCHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHH
MVLSAQFLFTILIFGSGFAIAVIPMVAQAYGRGDVVSVRRALRMGLWVVIAYWVIMQPAF
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
FNSEQILLFAQQKPEVAKLAHGYIMIGQFGVLPALLFNVMRALVSAIGKAGIILNVTIAT
CCCCEEEEEECCCCHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
LVLNAIFAYALVLGHFGFPAMGLEGAAIVSVVVQTAGFLFILAFVQKREETRRYEIFVRF
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
WKPDWHALLEVLRLGFPISVTVLAEVSLFTVASLLMGYIGTIELAAHGIALQWASIAFMI
ECCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
PLGLSQAATVRVGVAHGQGDYRGLVRASIMVLILACAISAVGSVLFATMPQFLGSWFLDV
HCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
NSPEAPAVLAYAGPLIVVAGLFQLVDGLQVIANGLLRGLKDARVPMIMALIAYWPIGFFL
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AWAFAFPLGFGGIGIWFGFLVGLAAAAVMLCWRFYLLLRREGAMAGA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]