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The map label for this gene is ddl [H]

Identifier: 209550169

GI number: 209550169

Start: 2628351

End: 2629277

Strand: Reverse

Name: ddl [H]

Synonym: Rleg2_2589

Alternate gene names: 209550169

Gene position: 2629277-2628351 (Counterclockwise)

Preceding gene: 209550170

Following gene: 209550168

Centisome position: 57.94

GC content: 58.36

Gene sequence:

>927_bases
ATGAGTCGCAAGCATGTCGCTGTCCTGATGGGCGGATTTTCCTCGGAACGGCCTGTCAGCCTTTCCTCGGGGAAGGCTTG
CGCAGAAGCCCTCGAAGCGGAAGGCTTCGACGTGACGCGCATCGATGTGGCGCGCGATGTTTCGGAAAAGCTTGCCGCCT
TGAAGCCCGATGTGGTCTTCAATGCGCTGCATGGCCCCTTCGGCGAGGACGGCACCATTCAGGGCATCCTCGAATATCTC
GAAATCCCCTATACGCATTCGGGCGTGCTTGCCTCGGCGCTGGCAATGGACAAGGACAAGGCGAAGCTTGTTGCTGCCGC
CGCCGGCATTCCGGTCGCCGAATCGCAGGTGATCAACCGTTTCGCCTTTCCCTCGACGCATCCGATGAAGCCTCCCTATG
TGGTGAAGCCGGTGCGGGAAGGGTCGAGCTTCGGCGTCGTCATTGTCACGGAAGATCAGGCGCATCCGCCGCAAGTCGTC
AGCTCGCCCGAGTGGCGCTACGGCGAGGAAGTGCTTGTCGAGCGCTATGTCTACGGCCGCGAACTCACCTGCGGCGTCAT
GGGCGAGACTGCGCTCGGCGTCACCGAGGTGGTGCCGCAGGGACACAATTTTTATGATTACGATTCCAAGTATGCGGCGG
GCGGTTCAAAACACGTCATCCCGGCAAAAATTTCACCGAATATTTACCAAAAAATACAAAGATTGGCCCTAAGGGCGCAT
CAGGCAATCGGTTGTCGCGGCGTCAGTCGGTCCGACTTTCGTTACGACGATCGTTTCACCGAAGACGGCGAAATCATCTG
GCTGGAAGTCAATACCCAGCCAGGCATGACTCCAACCTCGCTTGTGCCCGAAATGGCCGGTCATGCCGGTTATTCCTTTG
GTGAGTTTCTCCGGTGGATGGTGGAGGACGCGTCTTGTTTGCGTTGA

Upstream 100 bases:

>100_bases
GATGCTCTAATGCATGCAGGTCGGACAAAGGCCCGAAACGGCGCAGACAGCTTCGCGCAAGCGCCGATTTCGAGAATGAA
AGTTATTTTTGGGGGTGTTG

Downstream 100 bases:

>100_bases
CAGTCAAGAGGATAGGGCGCCCCAGCCATCATGCCGTGCTTCCGGTCATGGAAGCCGAAGAGCGATTCGTCCTGCCGCGT
CCGCTGCGCCGCGTTACCCG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSRKHVAVLMGGFSSERPVSLSSGKACAEALEAEGFDVTRIDVARDVSEKLAALKPDVVFNALHGPFGEDGTIQGILEYL
EIPYTHSGVLASALAMDKDKAKLVAAAAGIPVAESQVINRFAFPSTHPMKPPYVVKPVREGSSFGVVIVTEDQAHPPQVV
SSPEWRYGEEVLVERYVYGRELTCGVMGETALGVTEVVPQGHNFYDYDSKYAAGGSKHVIPAKISPNIYQKIQRLALRAH
QAIGCRGVSRSDFRYDDRFTEDGEIIWLEVNTQPGMTPTSLVPEMAGHAGYSFGEFLRWMVEDASCLR

Sequences:

>Translated_308_residues
MSRKHVAVLMGGFSSERPVSLSSGKACAEALEAEGFDVTRIDVARDVSEKLAALKPDVVFNALHGPFGEDGTIQGILEYL
EIPYTHSGVLASALAMDKDKAKLVAAAAGIPVAESQVINRFAFPSTHPMKPPYVVKPVREGSSFGVVIVTEDQAHPPQVV
SSPEWRYGEEVLVERYVYGRELTCGVMGETALGVTEVVPQGHNFYDYDSKYAAGGSKHVIPAKISPNIYQKIQRLALRAH
QAIGCRGVSRSDFRYDDRFTEDGEIIWLEVNTQPGMTPTSLVPEMAGHAGYSFGEFLRWMVEDASCLR
>Mature_307_residues
SRKHVAVLMGGFSSERPVSLSSGKACAEALEAEGFDVTRIDVARDVSEKLAALKPDVVFNALHGPFGEDGTIQGILEYLE
IPYTHSGVLASALAMDKDKAKLVAAAAGIPVAESQVINRFAFPSTHPMKPPYVVKPVREGSSFGVVIVTEDQAHPPQVVS
SPEWRYGEEVLVERYVYGRELTCGVMGETALGVTEVVPQGHNFYDYDSKYAAGGSKHVIPAKISPNIYQKIQRLALRAHQ
AIGCRGVSRSDFRYDDRFTEDGEIIWLEVNTQPGMTPTSLVPEMAGHAGYSFGEFLRWMVEDASCLR

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=309, Percent_Identity=39.1585760517799, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1786579, Length=355, Percent_Identity=30.7042253521127, Blast_Score=156, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33622; Mature: 33490

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKHVAVLMGGFSSERPVSLSSGKACAEALEAEGFDVTRIDVARDVSEKLAALKPDVVF
CCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHHH
NALHGPFGEDGTIQGILEYLEIPYTHSGVLASALAMDKDKAKLVAAAAGIPVAESQVINR
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHEEHHHCCCCCHHHHHHHH
FAFPSTHPMKPPYVVKPVREGSSFGVVIVTEDQAHPPQVVSSPEWRYGEEVLVERYVYGR
HCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
ELTCGVMGETALGVTEVVPQGHNFYDYDSKYAAGGSKHVIPAKISPNIYQKIQRLALRAH
CEEEEECCCHHCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
QAIGCRGVSRSDFRYDDRFTEDGEIIWLEVNTQPGMTPTSLVPEMAGHAGYSFGEFLRWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
VEDASCLR
HHHHHHCC
>Mature Secondary Structure 
SRKHVAVLMGGFSSERPVSLSSGKACAEALEAEGFDVTRIDVARDVSEKLAALKPDVVF
CCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCHHHH
NALHGPFGEDGTIQGILEYLEIPYTHSGVLASALAMDKDKAKLVAAAAGIPVAESQVINR
HHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHEEHHHCCCCCHHHHHHHH
FAFPSTHPMKPPYVVKPVREGSSFGVVIVTEDQAHPPQVVSSPEWRYGEEVLVERYVYGR
HCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCC
ELTCGVMGETALGVTEVVPQGHNFYDYDSKYAAGGSKHVIPAKISPNIYQKIQRLALRAH
CEEEEECCCHHCCHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
QAIGCRGVSRSDFRYDDRFTEDGEIIWLEVNTQPGMTPTSLVPEMAGHAGYSFGEFLRWM
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
VEDASCLR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]