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The map label for this gene is miaA [H]

Identifier: 209550119

GI number: 209550119

Start: 2568128

End: 2569021

Strand: Reverse

Name: miaA [H]

Synonym: Rleg2_2537

Alternate gene names: 209550119

Gene position: 2569021-2568128 (Counterclockwise)

Preceding gene: 209550121

Following gene: 209550117

Centisome position: 56.61

GC content: 66.78

Gene sequence:

>894_bases
ATGGAAAACCTTCTGAGCACAGTGAACGCGATCCTGATAACCGGGCCGACCGCCAGCGGCAAGTCCGCGCTTGCCGTTGA
ACTGGCCAAGCGCCATGGCGGCGCGGTCGTCAACGCCGACAGCATGCAGGTCTACGACACGTTGCGGGTGCTGACCGCGC
GCCCATCGGAAGTGGAAATGCAGGGTGTGCCGCACCATCTCTACGGCCACGTGCCGGCGGGTGCTGCCTATTCCACAGGC
GCCTGGCTGCGGGATGTCTCGGCGCTGTTGCCGGCGCTGAGGGCCGCCGGACAACTGCCGGTCTTCGTCGGCGGCACCGG
GCTTTATTTCAAGGCGCTGACCGGCGGCCTCTCCGATATGCCCGATATACCCGAGGCGCTGCGGGAAGAGTTGCGGGGGC
GTCTGCTGATGGAGGGGCCGGATAGGCTCCATGCAGAGCTTGCCGAGGTCGATCCTGCCATGGCGGCGGGCCTCAACCGC
CAGGACGGGCAGCGCATCGTCCGGGCGCTGGAGGTGGTGAAGGCAACGGGACGGTCGATCGCCGATTTCCAGGGCCAGTC
GGGACCGGTGGTGATCGACGCTGCCCAGGCTCGCAAGATCGTCGTCCTGCCGGAGCGGGCCGTGCTGCATGCCCGCATCA
ACGGCCGTTTCGAAAAGATGCTGCGCGAGAGAGCGGAAGACGAGGTGAGGGCGCTGCTTGCGCTCGGCCTGCCTGCCGAG
GCGCCTGTCATGAAGGCAATCGGCGTCTCGCAGATGGCGGCGATGCTGAGCGGCGAGATGACCCGCGACGACGTGCTGGA
GAAGGGTGCCGCGGCGACGCGGCAATATGCCAAGCGGCAGATGACGTGGTTCCGCAACCAGATGGACGAGAGCTGGGAGC
GGCTGACGCTTTAG

Upstream 100 bases:

>100_bases
GAGGCCGGAAGCGTTCACCGCTTCGGCCGCCTGTTCGGCGATCTTGGGTGTCAGCACGGGATTTGACGGATTGGCAACAA
GCGTGGCAACGAGAGCCATG

Downstream 100 bases:

>100_bases
CTGTGGGCCAGCCCGTCAGCCGCCGCACTTGTGCTGGGCATCGGGCAGAATCCAGCTTTCACCGGTGCGGCCGTTGGTGA
CGAGGAACAGTTTCCCGCCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MENLLSTVNAILITGPTASGKSALAVELAKRHGGAVVNADSMQVYDTLRVLTARPSEVEMQGVPHHLYGHVPAGAAYSTG
AWLRDVSALLPALRAAGQLPVFVGGTGLYFKALTGGLSDMPDIPEALREELRGRLLMEGPDRLHAELAEVDPAMAAGLNR
QDGQRIVRALEVVKATGRSIADFQGQSGPVVIDAAQARKIVVLPERAVLHARINGRFEKMLRERAEDEVRALLALGLPAE
APVMKAIGVSQMAAMLSGEMTRDDVLEKGAAATRQYAKRQMTWFRNQMDESWERLTL

Sequences:

>Translated_297_residues
MENLLSTVNAILITGPTASGKSALAVELAKRHGGAVVNADSMQVYDTLRVLTARPSEVEMQGVPHHLYGHVPAGAAYSTG
AWLRDVSALLPALRAAGQLPVFVGGTGLYFKALTGGLSDMPDIPEALREELRGRLLMEGPDRLHAELAEVDPAMAAGLNR
QDGQRIVRALEVVKATGRSIADFQGQSGPVVIDAAQARKIVVLPERAVLHARINGRFEKMLRERAEDEVRALLALGLPAE
APVMKAIGVSQMAAMLSGEMTRDDVLEKGAAATRQYAKRQMTWFRNQMDESWERLTL
>Mature_297_residues
MENLLSTVNAILITGPTASGKSALAVELAKRHGGAVVNADSMQVYDTLRVLTARPSEVEMQGVPHHLYGHVPAGAAYSTG
AWLRDVSALLPALRAAGQLPVFVGGTGLYFKALTGGLSDMPDIPEALREELRGRLLMEGPDRLHAELAEVDPAMAAGLNR
QDGQRIVRALEVVKATGRSIADFQGQSGPVVIDAAQARKIVVLPERAVLHARINGRFEKMLRERAEDEVRALLALGLPAE
APVMKAIGVSQMAAMLSGEMTRDDVLEKGAAATRQYAKRQMTWFRNQMDESWERLTL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=323, Percent_Identity=28.1733746130031, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1790613, Length=289, Percent_Identity=39.1003460207612, Blast_Score=191, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI25144712, Length=301, Percent_Identity=27.2425249169435, Blast_Score=91, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=27.3972602739726, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24649830, Length=343, Percent_Identity=25.0728862973761, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 32063; Mature: 32063

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLLSTVNAILITGPTASGKSALAVELAKRHGGAVVNADSMQVYDTLRVLTARPSEVEM
CCHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCC
QGVPHHLYGHVPAGAAYSTGAWLRDVSALLPALRAAGQLPVFVGGTGLYFKALTGGLSDM
CCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCCC
PDIPEALREELRGRLLMEGPDRLHAELAEVDPAMAAGLNRQDGQRIVRALEVVKATGRSI
CCCHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHH
ADFQGQSGPVVIDAAQARKIVVLPERAVLHARINGRFEKMLRERAEDEVRALLALGLPAE
HHCCCCCCCEEEECCCCCEEEEECCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APVMKAIGVSQMAAMLSGEMTRDDVLEKGAAATRQYAKRQMTWFRNQMDESWERLTL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MENLLSTVNAILITGPTASGKSALAVELAKRHGGAVVNADSMQVYDTLRVLTARPSEVEM
CCHHHHHHHEEEEECCCCCCCHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCC
QGVPHHLYGHVPAGAAYSTGAWLRDVSALLPALRAAGQLPVFVGGTGLYFKALTGGLSDM
CCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHCCCCCC
PDIPEALREELRGRLLMEGPDRLHAELAEVDPAMAAGLNRQDGQRIVRALEVVKATGRSI
CCCHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHCCCHH
ADFQGQSGPVVIDAAQARKIVVLPERAVLHARINGRFEKMLRERAEDEVRALLALGLPAE
HHCCCCCCCEEEECCCCCEEEEECCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APVMKAIGVSQMAAMLSGEMTRDDVLEKGAAATRQYAKRQMTWFRNQMDESWERLTL
CHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA