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The map label for this gene is ilvI [H]

Identifier: 209550115

GI number: 209550115

Start: 2562141

End: 2563931

Strand: Reverse

Name: ilvI [H]

Synonym: Rleg2_2532

Alternate gene names: 209550115

Gene position: 2563931-2562141 (Counterclockwise)

Preceding gene: 209550117

Following gene: 209550114

Centisome position: 56.5

GC content: 62.14

Gene sequence:

>1791_bases
ATGAGCACGGACAATCAGGCGGCAGGCAATCGGATGACGGGAGCGGAGATCGTTCTCAAGGCGCTGAAAGACAACGGCGT
CGAACATATCTTCGGCTATCCCGGCGGCGCGGTCCTGCCGATCTATGACGAGATCTTCCAGCAGGAAGAGGTCAAGCACA
TCCTCGTCCGTCACGAGCAGGGGGCAGGCCATGCGGCCGAAGGGTACGCCCGCTCCACCGGCAAAGTCGGCGTCATGCTG
GTCACCTCGGGCCCGGGCGCCACCAATGCGGTCACGCCGCTGCAGGATGCGCTGATGGATTCGATCCCGCTCGTCTGCCT
GACCGGTCAGGTTCCGACCCCGCTGATCGGCTCCGACGCCTTCCAGGAATGCGATACGGTCGGCATCACCCGGCCCTGCA
CCAAGCACAACTGGCTGGTCAAGGACGTCAACCAGCTCGCTGCCGTCATTCACGAGGCCTTCCGTATCGCGCAGTCCGGC
CGTCCGGGCCCGGTCGTCGTCGATATTCCGAAAGACGTGCAGTTTGCGACCGGCACCTACACGCCGCCGGCCGATTACGC
GATCCAGAAGAGCTATCAGCCGAAGATCCAGGGCGACCTCAACCAGATCCATGCGGCGATCGAGCTGATGGCGACTGCGC
GCCGTCCGATCATCTATTCCGGCGGCGGGGTCATCAATTCCGGCCCTGAGGCCTCCAAGCTGCTGCGCGAGCTGGTCGAG
CTCACCGATTTCCCGATCACCTCGACGCTGATGGGCCTCGGCGCCTACCCGGCTTCGGGCAAGAACTGGCTGAAGATGCT
CGGCATGCACGGCTCTTACGAGGCCAACATGGCGATGCACGACTGCGATGTCATGGTCTGCATCGGCGCCCGCTTCGACG
ACCGCATCACCGGCCGTCTCAACGCCTTTTCGCCGAACTCGAAGAAGATCCATATCGATGTCGATCCCTCTTCGATCAAC
AAGAATGTCCGCGTCGATATCGGCATCCGTGGCGATGTCGGCCATGTCCTCGAAGATATGGTTCGCTTGTGGCGGGCGCT
GCCGAAGAAGCCGGAGAAGAACCGTCTCGAAGGCTGGTGGACCGATATCGCCCGCTGGCGGGCGCGCAACTCCTTCGCCT
ATACGAGGAGCAACGATGTCATCATGCCGCAATATGCGCTGGAGCGGCTCTATGCGCATACCAAGGACCGCGATACCTAC
ATCACCACCGAAGTCGGCCAGCATCAGATGTGGGCGGCGCAGTTCTTCGGCTTCGAGCAGCCGAACCGTTGGATGACGTC
GGGTGGCCTTGGCACGATGGGCTACGGCCTGCCGGCCGCGCTCGGCGTGCAGATTGCCCATCCCGACAGCCTCGTCATCG
ATATTGCCGGCGACGCCTCGATCCAGATGTGCATCCAGGAAATGTCGGCGGCGATCCAGCACGATGCGCCGATCAAGATC
TTCATCATGAACAACCAGTACATGGGCATGGTGCGTCAGTGGCAGCAGCTGCTGCACGGCAACCGCCTGTCGAACTCCTA
TACGGAGGCGATGCCTGATTTCGTCAAGCTGGCGGAAGCCTATGGCGCCGTCGGCCTGCGCTGCGAAAAGCCGGATGAAC
TCGACGACGCCATTGTCGAGATGATCGAGGTCAGAAAGCCGGTGATCTTCGATTGCCGCGTTGCCAATCTCGCCAACTGC
TTCCCGATGATCCCCTCGGGCAAGGCGCATAACGAAATGCTGCTGCCTGATGAGGCTACCGACGAAGCGGTCGCCAATGC
GATCGACGCCAAGGGCCGCGCGCTCGTCTGA

Upstream 100 bases:

>100_bases
CCCATGCGCTAGATGACAGGCTCCTCCGGGGCCTTTTTTTATGCCCGAAATTCGGGCTTCCGGCCACAAACGCAAATCCC
AGCATAAACGGAACAGACAG

Downstream 100 bases:

>100_bases
CATTTTTAATTTGCGCATAATCCTTTCCGAAAATCGCTTCCGGTTTTCGGGGTTATGCGACAGGGAAGAGGAAACTCAAG
ACCATGAACGCACACCTACA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MSTDNQAAGNRMTGAEIVLKALKDNGVEHIFGYPGGAVLPIYDEIFQQEEVKHILVRHEQGAGHAAEGYARSTGKVGVML
VTSGPGATNAVTPLQDALMDSIPLVCLTGQVPTPLIGSDAFQECDTVGITRPCTKHNWLVKDVNQLAAVIHEAFRIAQSG
RPGPVVVDIPKDVQFATGTYTPPADYAIQKSYQPKIQGDLNQIHAAIELMATARRPIIYSGGGVINSGPEASKLLRELVE
LTDFPITSTLMGLGAYPASGKNWLKMLGMHGSYEANMAMHDCDVMVCIGARFDDRITGRLNAFSPNSKKIHIDVDPSSIN
KNVRVDIGIRGDVGHVLEDMVRLWRALPKKPEKNRLEGWWTDIARWRARNSFAYTRSNDVIMPQYALERLYAHTKDRDTY
ITTEVGQHQMWAAQFFGFEQPNRWMTSGGLGTMGYGLPAALGVQIAHPDSLVIDIAGDASIQMCIQEMSAAIQHDAPIKI
FIMNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGLRCEKPDELDDAIVEMIEVRKPVIFDCRVANLANC
FPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV

Sequences:

>Translated_596_residues
MSTDNQAAGNRMTGAEIVLKALKDNGVEHIFGYPGGAVLPIYDEIFQQEEVKHILVRHEQGAGHAAEGYARSTGKVGVML
VTSGPGATNAVTPLQDALMDSIPLVCLTGQVPTPLIGSDAFQECDTVGITRPCTKHNWLVKDVNQLAAVIHEAFRIAQSG
RPGPVVVDIPKDVQFATGTYTPPADYAIQKSYQPKIQGDLNQIHAAIELMATARRPIIYSGGGVINSGPEASKLLRELVE
LTDFPITSTLMGLGAYPASGKNWLKMLGMHGSYEANMAMHDCDVMVCIGARFDDRITGRLNAFSPNSKKIHIDVDPSSIN
KNVRVDIGIRGDVGHVLEDMVRLWRALPKKPEKNRLEGWWTDIARWRARNSFAYTRSNDVIMPQYALERLYAHTKDRDTY
ITTEVGQHQMWAAQFFGFEQPNRWMTSGGLGTMGYGLPAALGVQIAHPDSLVIDIAGDASIQMCIQEMSAAIQHDAPIKI
FIMNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGLRCEKPDELDDAIVEMIEVRKPVIFDCRVANLANC
FPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV
>Mature_595_residues
STDNQAAGNRMTGAEIVLKALKDNGVEHIFGYPGGAVLPIYDEIFQQEEVKHILVRHEQGAGHAAEGYARSTGKVGVMLV
TSGPGATNAVTPLQDALMDSIPLVCLTGQVPTPLIGSDAFQECDTVGITRPCTKHNWLVKDVNQLAAVIHEAFRIAQSGR
PGPVVVDIPKDVQFATGTYTPPADYAIQKSYQPKIQGDLNQIHAAIELMATARRPIIYSGGGVINSGPEASKLLRELVEL
TDFPITSTLMGLGAYPASGKNWLKMLGMHGSYEANMAMHDCDVMVCIGARFDDRITGRLNAFSPNSKKIHIDVDPSSINK
NVRVDIGIRGDVGHVLEDMVRLWRALPKKPEKNRLEGWWTDIARWRARNSFAYTRSNDVIMPQYALERLYAHTKDRDTYI
TTEVGQHQMWAAQFFGFEQPNRWMTSGGLGTMGYGLPAALGVQIAHPDSLVIDIAGDASIQMCIQEMSAAIQHDAPIKIF
IMNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGLRCEKPDELDDAIVEMIEVRKPVIFDCRVANLANCF
PMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=25.3521126760563, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI21361361, Length=568, Percent_Identity=24.8239436619718, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI87081685, Length=569, Percent_Identity=48.8576449912127, Blast_Score=565, Evalue=1e-162,
Organism=Escherichia coli, GI1790104, Length=578, Percent_Identity=40.6574394463668, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=33.5097001763668, Blast_Score=306, Evalue=3e-84,
Organism=Escherichia coli, GI1787096, Length=550, Percent_Identity=26.7272727272727, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI1788716, Length=567, Percent_Identity=24.8677248677249, Blast_Score=148, Evalue=8e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=570, Percent_Identity=26.140350877193, Blast_Score=143, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=570, Percent_Identity=26.140350877193, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=515, Percent_Identity=26.2135922330097, Blast_Score=131, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=44.253859348199, Blast_Score=498, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6320816, Length=490, Percent_Identity=23.8775510204082, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922626, Length=545, Percent_Identity=24.5871559633028, Blast_Score=154, Evalue=2e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 65411; Mature: 65279

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTDNQAAGNRMTGAEIVLKALKDNGVEHIFGYPGGAVLPIYDEIFQQEEVKHILVRHEQ
CCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCC
GAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCLTGQVPTPLIGSDA
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHH
FQECDTVGITRPCTKHNWLVKDVNQLAAVIHEAFRIAQSGRPGPVVVDIPKDVQFATGTY
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCC
TPPADYAIQKSYQPKIQGDLNQIHAAIELMATARRPIIYSGGGVINSGPEASKLLRELVE
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHH
LTDFPITSTLMGLGAYPASGKNWLKMLGMHGSYEANMAMHDCDVMVCIGARFDDRITGRL
HCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCCCEE
NAFSPNSKKIHIDVDPSSINKNVRVDIGIRGDVGHVLEDMVRLWRALPKKPEKNRLEGWW
ECCCCCCCEEEEEECHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TDIARWRARNSFAYTRSNDVIMPQYALERLYAHTKDRDTYITTEVGQHQMWAAQFFGFEQ
HHHHHHHHCCCEEEECCCCEECHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCC
PNRWMTSGGLGTMGYGLPAALGVQIAHPDSLVIDIAGDASIQMCIQEMSAAIQHDAPIKI
CCCEEECCCCCCCCCCCHHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEE
FIMNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGLRCEKPDELDDAIVE
EEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHH
MIEVRKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV
HHHHCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STDNQAAGNRMTGAEIVLKALKDNGVEHIFGYPGGAVLPIYDEIFQQEEVKHILVRHEQ
CCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHCCCC
GAGHAAEGYARSTGKVGVMLVTSGPGATNAVTPLQDALMDSIPLVCLTGQVPTPLIGSDA
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCHH
FQECDTVGITRPCTKHNWLVKDVNQLAAVIHEAFRIAQSGRPGPVVVDIPKDVQFATGTY
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCC
TPPADYAIQKSYQPKIQGDLNQIHAAIELMATARRPIIYSGGGVINSGPEASKLLRELVE
CCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHH
LTDFPITSTLMGLGAYPASGKNWLKMLGMHGSYEANMAMHDCDVMVCIGARFDDRITGRL
HCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCCCCCCCEE
NAFSPNSKKIHIDVDPSSINKNVRVDIGIRGDVGHVLEDMVRLWRALPKKPEKNRLEGWW
ECCCCCCCEEEEEECHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TDIARWRARNSFAYTRSNDVIMPQYALERLYAHTKDRDTYITTEVGQHQMWAAQFFGFEQ
HHHHHHHHCCCEEEECCCCEECHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHCCCCC
PNRWMTSGGLGTMGYGLPAALGVQIAHPDSLVIDIAGDASIQMCIQEMSAAIQHDAPIKI
CCCEEECCCCCCCCCCCHHHHEEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEE
FIMNNQYMGMVRQWQQLLHGNRLSNSYTEAMPDFVKLAEAYGAVGLRCEKPDELDDAIVE
EEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHH
MIEVRKPVIFDCRVANLANCFPMIPSGKAHNEMLLPDEATDEAVANAIDAKGRALV
HHHHCCCEEEEEEHHHHHHHCCCCCCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]