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The map label for this gene is smaIM [H]

Identifier: 209550029

GI number: 209550029

Start: 2492356

End: 2493495

Strand: Reverse

Name: smaIM [H]

Synonym: Rleg2_2446

Alternate gene names: 209550029

Gene position: 2493495-2492356 (Counterclockwise)

Preceding gene: 209550031

Following gene: 209550028

Centisome position: 54.95

GC content: 61.14

Gene sequence:

>1140_bases
ATGACCGAAACTCGCGTCCTTCTCGATGGCCGTTGCAGGATCCATGTCGGTGATTGCATTGAAGTGATGCGCTCGATGCC
GGCCAATTCGATCGACTGCGTTGTGACATCGCCGCCCTATTGGGGTCTTCGCGATTATGGCGTAGCCGGCCAGATCGGGC
TTGAGCGGACGCTTGGCGAGCACCTCGAGGTGATGGTTTCCGTTTTCCGAGAGATCCGCCGCATTCTCAAGGCTCATGGC
ACCGTTTGGGTCAATTATGGAGACTGCTACGCTGCCACCCCCAACGGGAAGAGTGCAGAAGCCTATAAGGCGGACGGCAG
CGACGACCGGACCTTTCGCGACAAGCCATTTTCGACGGTCGGCCCGGTCTATGACCCGAAGCATTCGAAAGCGCGGGGGC
TTTTTGTCTCTGGCGATCGCCAATCACACGTCGAAAGCGGCGGCCGTGTTGTCGCCGGCGGCTATATGAAGCCGAAGGAT
GTTTGTATGATCCCGAACCGGCTCGCGATTGCCCTGCAGGATGATGGTTGGTGGGTTCGCTCGGAGATCATCTGGAACAA
AACGAATCCGAAGCCCGAGTCCATTGATGACCGGCCGGCGGCGGTGCACGAGAAGATCTGGCTTCTGACAAAAAGCGAGC
GGTATTTCTTCGACGCCGACTCGCTGAAAGAGCCCACCACCGGCAACGCCCACGCGCGCCGCAAGGACGGCCAGTACAAG
GCGGCCAAAGGCAGCACCGCCGGGCAGAACCGGGAAGGGACGTGGGTCGAGACCTATGTGCCTGATCTTCGAAACGGCCG
GAACGTCTGGACCTTCAACATTGAGGGCTACCGCGGCGCCCATTTCGCCACGTTCCCACGCGAGCTCGCCCGCCGCTGCA
TCCTAGCTGGAACACCGAAGACGGTCTGTGGCTGCTGCGGCGCTGCCGAGGGCTGTGGCCCGATCTGCGACACGTTCGAG
CGTCTTCCCGGCATCGTGCTCGATCCCTTTGGTGGTTCTGGAACAGTAGCGGTGGTTGCCGAACAGTACAGCTACCGGAG
CGCGTTGATCGAGCTGAAGCCGGAATATGCCGACATCGCCGAGCGCCGCATCGCCGGCGCGCCAGTCGTCTCGATCGAGA
ACGAAGAGGAGGCGGCATGA

Upstream 100 bases:

>100_bases
GGGGCAAACTCATCGAATGTGTGCCATTTCATGCCGTTGGGTCTTTCCACGGTAAACCTATTGGTAACTGTGCAAAAACC
ATTCCCGTTGAGGTGGCGGC

Downstream 100 bases:

>100_bases
CCAGCCACAACAACAATGCGGCTCTAGGCAAACTCGGCGTTTTGATCCTCTCCCGCAAAATGCTCGTCGGCGTCTATGGC
CGCGGATGCCAAGTGAACAT

Product: DNA methylase N-4/N-6

Products: NA

Alternate protein names: M.SmaI; N-4 cytosine-specific methyltransferase SmaI [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MTETRVLLDGRCRIHVGDCIEVMRSMPANSIDCVVTSPPYWGLRDYGVAGQIGLERTLGEHLEVMVSVFREIRRILKAHG
TVWVNYGDCYAATPNGKSAEAYKADGSDDRTFRDKPFSTVGPVYDPKHSKARGLFVSGDRQSHVESGGRVVAGGYMKPKD
VCMIPNRLAIALQDDGWWVRSEIIWNKTNPKPESIDDRPAAVHEKIWLLTKSERYFFDADSLKEPTTGNAHARRKDGQYK
AAKGSTAGQNREGTWVETYVPDLRNGRNVWTFNIEGYRGAHFATFPRELARRCILAGTPKTVCGCCGAAEGCGPICDTFE
RLPGIVLDPFGGSGTVAVVAEQYSYRSALIELKPEYADIAERRIAGAPVVSIENEEEAA

Sequences:

>Translated_379_residues
MTETRVLLDGRCRIHVGDCIEVMRSMPANSIDCVVTSPPYWGLRDYGVAGQIGLERTLGEHLEVMVSVFREIRRILKAHG
TVWVNYGDCYAATPNGKSAEAYKADGSDDRTFRDKPFSTVGPVYDPKHSKARGLFVSGDRQSHVESGGRVVAGGYMKPKD
VCMIPNRLAIALQDDGWWVRSEIIWNKTNPKPESIDDRPAAVHEKIWLLTKSERYFFDADSLKEPTTGNAHARRKDGQYK
AAKGSTAGQNREGTWVETYVPDLRNGRNVWTFNIEGYRGAHFATFPRELARRCILAGTPKTVCGCCGAAEGCGPICDTFE
RLPGIVLDPFGGSGTVAVVAEQYSYRSALIELKPEYADIAERRIAGAPVVSIENEEEAA
>Mature_378_residues
TETRVLLDGRCRIHVGDCIEVMRSMPANSIDCVVTSPPYWGLRDYGVAGQIGLERTLGEHLEVMVSVFREIRRILKAHGT
VWVNYGDCYAATPNGKSAEAYKADGSDDRTFRDKPFSTVGPVYDPKHSKARGLFVSGDRQSHVESGGRVVAGGYMKPKDV
CMIPNRLAIALQDDGWWVRSEIIWNKTNPKPESIDDRPAAVHEKIWLLTKSERYFFDADSLKEPTTGNAHARRKDGQYKA
AKGSTAGQNREGTWVETYVPDLRNGRNVWTFNIEGYRGAHFATFPRELARRCILAGTPKTVCGCCGAAEGCGPICDTFER
LPGIVLDPFGGSGTVAVVAEQYSYRSALIELKPEYADIAERRIAGAPVVSIENEEEAA

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the SmaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 41854; Mature: 41722

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETRVLLDGRCRIHVGDCIEVMRSMPANSIDCVVTSPPYWGLRDYGVAGQIGLERTLGE
CCCCEEEECCCEEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
HLEVMVSVFREIRRILKAHGTVWVNYGDCYAATPNGKSAEAYKADGSDDRTFRDKPFSTV
HHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCC
GPVYDPKHSKARGLFVSGDRQSHVESGGRVVAGGYMKPKDVCMIPNRLAIALQDDGWWVR
CCCCCCCCCCCCEEEEECCCHHHHHCCCEEEECCCCCCCCEEECCCEEEEEEECCCEEEE
SEIIWNKTNPKPESIDDRPAAVHEKIWLLTKSERYFFDADSLKEPTTGNAHARRKDGQYK
EEEEECCCCCCCCCCCCCCHHHCCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCEE
AAKGSTAGQNREGTWVETYVPDLRNGRNVWTFNIEGYRGAHFATFPRELARRCILAGTPK
ECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEECCCCH
TVCGCCGAAEGCGPICDTFERLPGIVLDPFGGSGTVAVVAEQYSYRSALIELKPEYADIA
HHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCEEEEEEEHHCCCEEEEEECCCHHHHH
ERRIAGAPVVSIENEEEAA
HHHHCCCCEEEECCCCCCC
>Mature Secondary Structure 
TETRVLLDGRCRIHVGDCIEVMRSMPANSIDCVVTSPPYWGLRDYGVAGQIGLERTLGE
CCCEEEECCCEEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHH
HLEVMVSVFREIRRILKAHGTVWVNYGDCYAATPNGKSAEAYKADGSDDRTFRDKPFSTV
HHHHHHHHHHHHHHHHHHCCEEEEEECCEEEECCCCCCCCEEECCCCCCCCCCCCCCCCC
GPVYDPKHSKARGLFVSGDRQSHVESGGRVVAGGYMKPKDVCMIPNRLAIALQDDGWWVR
CCCCCCCCCCCCEEEEECCCHHHHHCCCEEEECCCCCCCCEEECCCEEEEEEECCCEEEE
SEIIWNKTNPKPESIDDRPAAVHEKIWLLTKSERYFFDADSLKEPTTGNAHARRKDGQYK
EEEEECCCCCCCCCCCCCCHHHCCEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCEE
AAKGSTAGQNREGTWVETYVPDLRNGRNVWTFNIEGYRGAHFATFPRELARRCILAGTPK
ECCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHEECCCCH
TVCGCCGAAEGCGPICDTFERLPGIVLDPFGGSGTVAVVAEQYSYRSALIELKPEYADIA
HHHHHCCCCCCCCHHHHHHHHCCCEEEECCCCCCEEEEEEEHHCCCEEEEEECCCHHHHH
ERRIAGAPVVSIENEEEAA
HHHHCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2690008; 2251121 [H]