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The map label for this gene is arrD [H]

Identifier: 209549987

GI number: 209549987

Start: 2462234

End: 2462698

Strand: Reverse

Name: arrD [H]

Synonym: Rleg2_2404

Alternate gene names: 209549987

Gene position: 2462698-2462234 (Counterclockwise)

Preceding gene: 209549988

Following gene: 209549986

Centisome position: 54.27

GC content: 63.87

Gene sequence:

>465_bases
ATGGCAAACCGCCTTCAGAAGGGTAGCGCCGCGGCTGCAATGGCCGTGGCATTGGTCGGCAGCTTCGAGGGGCTTCGTCA
GAGTGCCTATCCAGATCCGGCAACGCAGGGGCAGCCGTGGACGATCTGCTACGGCTCAACGAACGGCGTTAAGCCGGGCG
ACCGCAAGACGGTGGAACAGTGCAAAGCGCTGCTGGCGCTCGAGCTGCAGACCTATGCCGCGGGCATCGATCATTGCGTC
GCCGTTCCGCTGCCGGATGCTCGCTTCGTGGCGCTGACCTCCTTCGCCTACAATGTCGGGGTCAAGGCAGCCTGCGGATC
GAGCGCCGTCAAACTCATCAACAAGGGCAAGACGGCTGAGGGCTGCGAGGCTCTGCTGAAGTGGAACCGCGCCGCCGGCA
TCGTCTTTCCCGGGCTGACCCGCCGTCGGCAAAAGGAACGCCAATTCTGCCTTGAGGACCTGTGA

Upstream 100 bases:

>100_bases
TTCTATGTCCGCCTCGCAATGTCGGCGCTTACCGGCATCATCGCCGCCGCAGCCCTCCTGGCGCGGTTCTTCGTTCAACA
GAAACTCTCAGGGGATTTGA

Downstream 100 bases:

>100_bases
TGTTCGGAATCCTCGATTATGCCAAGCTTGGCGCCGGCGTCGCGGCCGGAATCCTCTTTTATCATGTCTACGCCGTCGCC
ATCGGCTACCCTTCCGCGGC

Product: glycoside hydrolase family 24

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MANRLQKGSAAAAMAVALVGSFEGLRQSAYPDPATQGQPWTICYGSTNGVKPGDRKTVEQCKALLALELQTYAAGIDHCV
AVPLPDARFVALTSFAYNVGVKAACGSSAVKLINKGKTAEGCEALLKWNRAAGIVFPGLTRRRQKERQFCLEDL

Sequences:

>Translated_154_residues
MANRLQKGSAAAAMAVALVGSFEGLRQSAYPDPATQGQPWTICYGSTNGVKPGDRKTVEQCKALLALELQTYAAGIDHCV
AVPLPDARFVALTSFAYNVGVKAACGSSAVKLINKGKTAEGCEALLKWNRAAGIVFPGLTRRRQKERQFCLEDL
>Mature_153_residues
ANRLQKGSAAAAMAVALVGSFEGLRQSAYPDPATQGQPWTICYGSTNGVKPGDRKTVEQCKALLALELQTYAAGIDHCVA
VPLPDARFVALTSFAYNVGVKAACGSSAVKLINKGKTAEGCEALLKWNRAAGIVFPGLTRRRQKERQFCLEDL

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=131, Percent_Identity=35.8778625954198, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 16403; Mature: 16272

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQKGSAAAAMAVALVGSFEGLRQSAYPDPATQGQPWTICYGSTNGVKPGDRKTVEQ
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
CKALLALELQTYAAGIDHCVAVPLPDARFVALTSFAYNVGVKAACGSSAVKLINKGKTAE
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHH
GCEALLKWNRAAGIVFPGLTRRRQKERQFCLEDL
HHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANRLQKGSAAAAMAVALVGSFEGLRQSAYPDPATQGQPWTICYGSTNGVKPGDRKTVEQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCHHHHHH
CKALLALELQTYAAGIDHCVAVPLPDARFVALTSFAYNVGVKAACGSSAVKLINKGKTAE
HHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHH
GCEALLKWNRAAGIVFPGLTRRRQKERQFCLEDL
HHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]