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The map label for this gene is pphA [H]

Identifier: 209549928

GI number: 209549928

Start: 2404861

End: 2405502

Strand: Reverse

Name: pphA [H]

Synonym: Rleg2_2344

Alternate gene names: 209549928

Gene position: 2405502-2404861 (Counterclockwise)

Preceding gene: 209549929

Following gene: 209549925

Centisome position: 53.01

GC content: 57.79

Gene sequence:

>642_bases
ATGCGCTACACGTTCGCCATCGGTGATATCCACGGCTGTATCGATCCACTGAACAGCATGATCGATCGCATGGAGGCTTA
TGCGTCTGAAGGTACAGTCGTTTTCCTTGGCGACTATATCGATCGCGGGCCAGACAGCAGACGTGTCCTCGACCGGCTCA
TTGCCGGTCCGCCCGGCCCGTGGCGCTGGATCTGCTTGAAAGGCAATCATGAGGACATGATGGTCGCTGCCTATGCCGAC
AGCGACGACAGAGCCGTATGGATCAGCAATGGCGGGCTTGAAACCGAAATGTCCTATGATGGCAGGGTTCTGCCTCAGCA
TTTGCAATGGGCTGGCGATCGTCCGCTTATGCATGTGGATCGATATCGCATCTTTGTCCATGCCGGCGTTGTTCCGGAAT
TTCCGCTGGAACGGCAGACCAAGCGCGATCTTCTCTGGCTGCGGTTCCCTCCCGGTCAATCCAGCGACTATTGGGGCAAA
CATCTGGTCCACGGCCATACTCCCTCACTGTCCAACCCGGTGACGACAGGAAACCGCACCAACATCGACAGCGGCTGCGT
CTTCGGCGGCAAGCTTTCCTGTGCCGTGTTCGACGATGAGGTTGCCGGCGGGCCGATCGACTTTATCGAGGTGAGGGCAT
GA

Upstream 100 bases:

>100_bases
TAAGTGCCAGGAAATGAGTCGGATTTTCTGTCGGCAGTATTGAGATCCGGCGAGGCGATCCGGACTTCACCTGGCTTCCA
ACAGGTTCTAAAAATGAAGC

Downstream 100 bases:

>100_bases
ACCTGCTCACGTCCGTAAGGGTGTAAAGCCAGAGACGGCGGAGTGGAGCAATGCGAAACGCGGGATTTATCGGGCCGCCA
GCAGAAGGTTTGGCGAGAAC

Product: bis(5'nucleosyl)-tetraphosphatase ApaH

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MRYTFAIGDIHGCIDPLNSMIDRMEAYASEGTVVFLGDYIDRGPDSRRVLDRLIAGPPGPWRWICLKGNHEDMMVAAYAD
SDDRAVWISNGGLETEMSYDGRVLPQHLQWAGDRPLMHVDRYRIFVHAGVVPEFPLERQTKRDLLWLRFPPGQSSDYWGK
HLVHGHTPSLSNPVTTGNRTNIDSGCVFGGKLSCAVFDDEVAGGPIDFIEVRA

Sequences:

>Translated_213_residues
MRYTFAIGDIHGCIDPLNSMIDRMEAYASEGTVVFLGDYIDRGPDSRRVLDRLIAGPPGPWRWICLKGNHEDMMVAAYAD
SDDRAVWISNGGLETEMSYDGRVLPQHLQWAGDRPLMHVDRYRIFVHAGVVPEFPLERQTKRDLLWLRFPPGQSSDYWGK
HLVHGHTPSLSNPVTTGNRTNIDSGCVFGGKLSCAVFDDEVAGGPIDFIEVRA
>Mature_213_residues
MRYTFAIGDIHGCIDPLNSMIDRMEAYASEGTVVFLGDYIDRGPDSRRVLDRLIAGPPGPWRWICLKGNHEDMMVAAYAD
SDDRAVWISNGGLETEMSYDGRVLPQHLQWAGDRPLMHVDRYRIFVHAGVVPEFPLERQTKRDLLWLRFPPGQSSDYWGK
HLVHGHTPSLSNPVTTGNRTNIDSGCVFGGKLSCAVFDDEVAGGPIDFIEVRA

Specific function: Plays a key role in signaling protein misfolding via the CpxR/CPXA transducing system. It also modulates the phosphorylated status of many phosphoproteins in E.coli, some of which acting as major chaperones. Has been shown, in vitro, to act on Ser, Thr an

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081988, Length=208, Percent_Identity=31.25, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.1.3.16 [H]

Molecular weight: Translated: 23859; Mature: 23859

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00125 SER_THR_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYTFAIGDIHGCIDPLNSMIDRMEAYASEGTVVFLGDYIDRGPDSRRVLDRLIAGPPGP
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCC
WRWICLKGNHEDMMVAAYADSDDRAVWISNGGLETEMSYDGRVLPQHLQWAGDRPLMHVD
EEEEEEECCCCCEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCHHHHCCCCCCCEEEE
RYRIFVHAGVVPEFPLERQTKRDLLWLRFPPGQSSDYWGKHLVHGHTPSLSNPVTTGNRT
EEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
NIDSGCVFGGKLSCAVFDDEVAGGPIDFIEVRA
CCCCCEEECCEEEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MRYTFAIGDIHGCIDPLNSMIDRMEAYASEGTVVFLGDYIDRGPDSRRVLDRLIAGPPGP
CEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCC
WRWICLKGNHEDMMVAAYADSDDRAVWISNGGLETEMSYDGRVLPQHLQWAGDRPLMHVD
EEEEEEECCCCCEEEEEEECCCCCEEEEECCCEEEEECCCCCCCCHHHHCCCCCCCEEEE
RYRIFVHAGVVPEFPLERQTKRDLLWLRFPPGQSSDYWGKHLVHGHTPSLSNPVTTGNRT
EEEEEEEECCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCC
NIDSGCVFGGKLSCAVFDDEVAGGPIDFIEVRA
CCCCCEEECCEEEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9130712; 9097040; 9278503 [H]