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The map label for this gene is glnG [H]

Identifier: 209549920

GI number: 209549920

Start: 2397149

End: 2397550

Strand: Reverse

Name: glnG [H]

Synonym: Rleg2_2336

Alternate gene names: 209549920

Gene position: 2397550-2397149 (Counterclockwise)

Preceding gene: 209549921

Following gene: 209549918

Centisome position: 52.83

GC content: 58.96

Gene sequence:

>402_bases
ATGGTTACAGTCCTGTGCATAGAAGATGAAGTCGAGATCCGGAACCTCCTCGTCGAAGAGTTGACCGAAGCCGGCTACAG
GACGCTCGAGGCCTCGAACGGCGCCGAAGGGCTGGAAATGATCCTGTCGAAATGGCCCGACATCGTCATCAGCGATATTT
CCATGCCGGTCATGGACGGGCACCAGCTTCTGGCCGAAATTCAGATCAATCACGCCGAGCTCTCCAACATCCCCTTCATC
CTGCTGACGGCGCTGACCGACCGCGAAAACACGCTTGCCGGCCTTCGCGCCGGTGCGGCGGACTATCTGACCAAGCCGCT
CGATTTCGACCTGTTGCTCGCCAAGCTGGAGGGCTGCGTGACGCGGCTGGAGAATGACAAGGCGGTCAATCGGTCGTTTT
GA

Upstream 100 bases:

>100_bases
GAGGTGACGAGCGAGCTCGGCAAGGGCACAACATTTATCATTCGTCTGCCAATAGGGTCTCTATTGTCGGATCAACACGT
CGCAGCTTAGGGAAAAGCTT

Downstream 100 bases:

>100_bases
AGGGGCTTGAGCGATGATGGAGAGTGTGCCGTGCGGCCCTGGACCGGTATCGACTAGCGCCTAACCTCTCCTCCTTCATT
CCTGTGCTTGTCACAGGAAT

Product: response regulator receiver protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 133

Protein sequence:

>133_residues
MVTVLCIEDEVEIRNLLVEELTEAGYRTLEASNGAEGLEMILSKWPDIVISDISMPVMDGHQLLAEIQINHAELSNIPFI
LLTALTDRENTLAGLRAGAADYLTKPLDFDLLLAKLEGCVTRLENDKAVNRSF

Sequences:

>Translated_133_residues
MVTVLCIEDEVEIRNLLVEELTEAGYRTLEASNGAEGLEMILSKWPDIVISDISMPVMDGHQLLAEIQINHAELSNIPFI
LLTALTDRENTLAGLRAGAADYLTKPLDFDLLLAKLEGCVTRLENDKAVNRSF
>Mature_133_residues
MVTVLCIEDEVEIRNLLVEELTEAGYRTLEASNGAEGLEMILSKWPDIVISDISMPVMDGHQLLAEIQINHAELSNIPFI
LLTALTDRENTLAGLRAGAADYLTKPLDFDLLLAKLEGCVTRLENDKAVNRSF

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=37.9032258064516, Blast_Score=83, Evalue=8e-18,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=36.697247706422, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1786599, Length=120, Percent_Identity=29.1666666666667, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1786911, Length=122, Percent_Identity=34.4262295081967, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1790437, Length=117, Percent_Identity=36.7521367521368, Blast_Score=69, Evalue=1e-13,
Organism=Escherichia coli, GI87082012, Length=130, Percent_Identity=33.8461538461538, Blast_Score=68, Evalue=2e-13,
Organism=Escherichia coli, GI1786784, Length=119, Percent_Identity=34.453781512605, Blast_Score=67, Evalue=6e-13,
Organism=Escherichia coli, GI1788905, Length=114, Percent_Identity=37.719298245614, Blast_Score=65, Evalue=1e-12,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI1788394, Length=129, Percent_Identity=28.6821705426357, Blast_Score=63, Evalue=5e-12,
Organism=Escherichia coli, GI1787375, Length=119, Percent_Identity=30.2521008403361, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1790863, Length=123, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 14712; Mature: 14712

Theoretical pI: Translated: 4.08; Mature: 4.08

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVLCIEDEVEIRNLLVEELTEAGYRTLEASNGAEGLEMILSKWPDIVISDISMPVMDG
CEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCEEEECCCCCCHHH
HQLLAEIQINHAELSNIPFILLTALTDRENTLAGLRAGAADYLTKPLDFDLLLAKLEGCV
HHHHHHHHCCHHHHCCCCEEEEEEECCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHH
TRLENDKAVNRSF
HHHCCCCCCCCCC
>Mature Secondary Structure
MVTVLCIEDEVEIRNLLVEELTEAGYRTLEASNGAEGLEMILSKWPDIVISDISMPVMDG
CEEEEEECCCHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCEEEECCCCCCHHH
HQLLAEIQINHAELSNIPFILLTALTDRENTLAGLRAGAADYLTKPLDFDLLLAKLEGCV
HHHHHHHHCCHHHHCCCCEEEEEEECCCCHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHH
TRLENDKAVNRSF
HHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]