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The map label for this gene is argG1 [H]

Identifier: 209549819

GI number: 209549819

Start: 2286291

End: 2287505

Strand: Direct

Name: argG1 [H]

Synonym: Rleg2_2231

Alternate gene names: 209549819

Gene position: 2286291-2287505 (Clockwise)

Preceding gene: 209549817

Following gene: 209549820

Centisome position: 50.38

GC content: 61.23

Gene sequence:

>1215_bases
ATGGCAAGACAGATAGATAAAATCGTGCTCGCTTATTCCGGCGGCTTGGATACCTCGATCATCCTCAAATGGCTGCAGGA
GACCTACGGATGCGAGGTCGTGACCTTCACCGCCGATCTCGGGCAGGGCGAGGAGCTTGAGCCGGCGCGGGCGAAGGCGG
CAATGTTGGGGGTCAAGGACATCCGTATCGTCGACCTGCGCGAGGAATTCGTTCGTGACTTCGTCTTTCCGATGCTGCGC
GCCAACACGCTTTATGAGGGACAGTATCTGCTCGGCAGCTCCATCGCCCGGCCGCTGATAGCCAAGCATCTGGTCGCCAT
AGCCCGGCAAATCGGCGCTGATGCCGTTGCCCACGGCTCGACAGGCAAGGGCAATGACCAGGTCCGGTTCGAACTTGCCG
TAAATGCGCTCGATCCGTCGATTAAGGTCATTGCTCTCTGGCGCCAATGGAACATCCGCTCCCGCATGCAGCTTCTGGAA
TATGCCGAGATGCATCGCATCCCGGTGCCGAAGGACAAGCGCGGTGAAGCGCCCTTCTCGATGGACGCCAACCTGCTGCA
CACGTCCACCGAAGGCAAAATCCTCGAAGATCCGGCCGAGGTTGCGCCCGAATACATCTATCAGCGCACGGTCGACCCCG
TCGATGCGCCCGATACCCCCGAGACCGCGACCATCGGCTTCGAAAACGGCGACCCCGTATCCGTCAACGGGAAGGCCATG
ACACCCGCTGCGTTGCTGACCGAGCTCAACAGCTTCGGCGGCCGGCACGGCGTCGGGCGGCTCGATCTCGTCGAAAACCG
CTTCATCGGCATGAAGTCGCGAGGCGTCTATGAGACGCCGGGAGGCACGATCCTGCTTGCGGCGCACCGAGGCATTGAAT
CGATCACGCTCGATCGTGCCGCCGCACACCTGAAGGATGAGATCATGCCCCGCTACGCCGAGCTGATCTACAACGGCTTC
TGGTTCTCTCCCGAGCGGGACATGCTGCAGGCTCTGATCGACCGGAGCCAGGACTTCGTCAGCGGCGAAGTGACGGTACG
GCTCTATAAGGGAAGCGCTACGGTCATCTCCCGCACCTCGCCCTGCTCACTCTATTCCGCCGACCTCGTCACCTTCGAGG
AAAGCGCCATCGCCTATGACCATCACGACGCCGAAGGCTTCATCAAACTCAACGGATTGCGGCTCAGGAGCTGGGCAGCC
CGCAACGGCAGATGA

Upstream 100 bases:

>100_bases
ATAGCATAATATGCCAAATATTCGCAATTTTGAGGCCTCTTCGCAAGCTCATTGCGCAAGACATGCTCTATTGTTGCAAG
CATTGGTGTGGAGACGGCTG

Downstream 100 bases:

>100_bases
GCCCTGCCTTGCGTCGGCGCGTTGGCAGCCACGACCCGAATCCGATTTCAAACGATCTTAAAAATACGCACGAAAAAACT
AATCGATTTTAATTTTATCA

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase 1 [H]

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MARQIDKIVLAYSGGLDTSIILKWLQETYGCEVVTFTADLGQGEELEPARAKAAMLGVKDIRIVDLREEFVRDFVFPMLR
ANTLYEGQYLLGSSIARPLIAKHLVAIARQIGADAVAHGSTGKGNDQVRFELAVNALDPSIKVIALWRQWNIRSRMQLLE
YAEMHRIPVPKDKRGEAPFSMDANLLHTSTEGKILEDPAEVAPEYIYQRTVDPVDAPDTPETATIGFENGDPVSVNGKAM
TPAALLTELNSFGGRHGVGRLDLVENRFIGMKSRGVYETPGGTILLAAHRGIESITLDRAAAHLKDEIMPRYAELIYNGF
WFSPERDMLQALIDRSQDFVSGEVTVRLYKGSATVISRTSPCSLYSADLVTFEESAIAYDHHDAEGFIKLNGLRLRSWAA
RNGR

Sequences:

>Translated_404_residues
MARQIDKIVLAYSGGLDTSIILKWLQETYGCEVVTFTADLGQGEELEPARAKAAMLGVKDIRIVDLREEFVRDFVFPMLR
ANTLYEGQYLLGSSIARPLIAKHLVAIARQIGADAVAHGSTGKGNDQVRFELAVNALDPSIKVIALWRQWNIRSRMQLLE
YAEMHRIPVPKDKRGEAPFSMDANLLHTSTEGKILEDPAEVAPEYIYQRTVDPVDAPDTPETATIGFENGDPVSVNGKAM
TPAALLTELNSFGGRHGVGRLDLVENRFIGMKSRGVYETPGGTILLAAHRGIESITLDRAAAHLKDEIMPRYAELIYNGF
WFSPERDMLQALIDRSQDFVSGEVTVRLYKGSATVISRTSPCSLYSADLVTFEESAIAYDHHDAEGFIKLNGLRLRSWAA
RNGR
>Mature_403_residues
ARQIDKIVLAYSGGLDTSIILKWLQETYGCEVVTFTADLGQGEELEPARAKAAMLGVKDIRIVDLREEFVRDFVFPMLRA
NTLYEGQYLLGSSIARPLIAKHLVAIARQIGADAVAHGSTGKGNDQVRFELAVNALDPSIKVIALWRQWNIRSRMQLLEY
AEMHRIPVPKDKRGEAPFSMDANLLHTSTEGKILEDPAEVAPEYIYQRTVDPVDAPDTPETATIGFENGDPVSVNGKAMT
PAALLTELNSFGGRHGVGRLDLVENRFIGMKSRGVYETPGGTILLAAHRGIESITLDRAAAHLKDEIMPRYAELIYNGFW
FSPERDMLQALIDRSQDFVSGEVTVRLYKGSATVISRTSPCSLYSADLVTFEESAIAYDHHDAEGFIKLNGLRLRSWAAR
NGR

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI53759107, Length=400, Percent_Identity=48.5, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI16950633, Length=400, Percent_Identity=48.5, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1789563, Length=410, Percent_Identity=29.0243902439024, Blast_Score=134, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6324514, Length=407, Percent_Identity=42.2604422604423, Blast_Score=314, Evalue=1e-86,
Organism=Drosophila melanogaster, GI21358151, Length=404, Percent_Identity=45.5445544554455, Blast_Score=339, Evalue=2e-93,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00764 Arginosuc_synth [H]

EC number: =6.3.4.5 [H]

Molecular weight: Translated: 44737; Mature: 44605

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1 ; PS00565 ARGININOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQIDKIVLAYSGGLDTSIILKWLQETYGCEVVTFTADLGQGEELEPARAKAAMLGVKD
CCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCCCC
IRIVDLREEFVRDFVFPMLRANTLYEGQYLLGSSIARPLIAKHLVAIARQIGADAVAHGS
EEEEHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEECCC
TGKGNDQVRFELAVNALDPSIKVIALWRQWNIRSRMQLLEYAEMHRIPVPKDKRGEAPFS
CCCCCCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
MDANLLHTSTEGKILEDPAEVAPEYIYQRTVDPVDAPDTPETATIGFENGDPVSVNGKAM
CCCCEEEECCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCC
TPAALLTELNSFGGRHGVGRLDLVENRFIGMKSRGVYETPGGTILLAAHRGIESITLDRA
CHHHHHHHHHHCCCCCCCCCEEEHHCCEECCCCCCCEECCCCEEEEEECCCCCHHHHHHH
AAHLKDEIMPRYAELIYNGFWFSPERDMLQALIDRSQDFVSGEVTVRLYKGSATVISRTS
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEECCC
PCSLYSADLVTFEESAIAYDHHDAEGFIKLNGLRLRSWAARNGR
CCCEEECCEEEEECCEEEECCCCCCCEEEECCEEEEECCCCCCC
>Mature Secondary Structure 
ARQIDKIVLAYSGGLDTSIILKWLQETYGCEVVTFTADLGQGEELEPARAKAAMLGVKD
CCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHCCCCC
IRIVDLREEFVRDFVFPMLRANTLYEGQYLLGSSIARPLIAKHLVAIARQIGADAVAHGS
EEEEHHHHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHCCHHEECCC
TGKGNDQVRFELAVNALDPSIKVIALWRQWNIRSRMQLLEYAEMHRIPVPKDKRGEAPFS
CCCCCCEEEEEEEEECCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
MDANLLHTSTEGKILEDPAEVAPEYIYQRTVDPVDAPDTPETATIGFENGDPVSVNGKAM
CCCCEEEECCCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCCC
TPAALLTELNSFGGRHGVGRLDLVENRFIGMKSRGVYETPGGTILLAAHRGIESITLDRA
CHHHHHHHHHHCCCCCCCCCEEEHHCCEECCCCCCCEECCCCEEEEEECCCCCHHHHHHH
AAHLKDEIMPRYAELIYNGFWFSPERDMLQALIDRSQDFVSGEVTVRLYKGSATVISRTS
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCEEEEECCC
PCSLYSADLVTFEESAIAYDHHDAEGFIKLNGLRLRSWAARNGR
CCCEEECCEEEEECCEEEECCCCCCCEEEECCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA