Click here to switch to the map view.

The map label for this gene is kefA [C]

Identifier: 209549755

GI number: 209549755

Start: 2217024

End: 2219555

Strand: Reverse

Name: kefA [C]

Synonym: Rleg2_2163

Alternate gene names: 209549755

Gene position: 2219555-2217024 (Counterclockwise)

Preceding gene: 209549756

Following gene: 209549754

Centisome position: 48.91

GC content: 63.11

Gene sequence:

>2532_bases
TTGCGGCTGAGGTCCATCCTGCTTCGCACCATTCTTCTCGTGACGCTGGCGCTCGCCGGCGCGCATTTCGAGGGTGCCGC
GGCCTTCGCGCAGGAAGCGCAGACGCAGGCGGCCGCGCCGGCGCCTGTCGATACCCCGTTCGACCAGGCGGCGAAGGAGA
TCGACAAGGCGAAGGTTCAGCTGACGGCGCTTCAGGACGGCGTCAAGCAGAATGCCGACAATGACGATGCGCTGGTCGGG
CTTGCGACCAAGGCCGACGAGCTCAGCCGCGCCGTCATCACCATATCGGTCAATCTCAGGCCGCGTTTCGACCAGATCAA
GAACCGGCTTGCCGAGATCGGCGATCCGCCGAAGGACGGGCAGCCTCCCGAAGCCGAGATCGTCACCCAGGAGCGCAATA
CGCTCGCCGCCGAGCGGGCGCAGATCAACGCGGTGACGGGCGACGCCGAAAACCTGTCGATTGCGGTGACGAAGCTCGTC
AACCAGATCATCGAGATGCGGCGGCAGTTGTTTGCCGATACGCTTTTCAAGCGGACCGAGGTCTCCGCTTCGGTGATCGA
CGATGCGGCCGGCGCCTTCGTGCACGAGATCAGCGAATTCTCGCAGGCTCTGTCGAGCTGGGCCGTCTTCGTCTGGAAAT
TCAAGCGTTTTCCGATGTTTGCCGCGATCTTCCTGTCGCTCGCCTCGGCGCTCATCCTGCTTTCCGGCAGCTACCGGCTG
TTCGGCGCATATCTGCGCCGCGATGAGGCGGTCGAGGAGCCGTCCTATATCGGCCGGCTGTCGATTGCCTTCTGGTCGAC
GCTGATCCGTACGATGGCGCTGTCAGTGCTGCTTGTCACCTCGTTCTTCTTCCTCAACGGCTTCAACGTGCTGAGGCCGG
ATATCGCGCCGGTGATCGGTGCGTTGTTCGGAACGATTGGGCTCGTCTATTTCGTCGGCCGCTTCGCCAACGCCATCTTC
GCGCCGCGCGAGCCGCGCTGGCGGCTTGTGCGGCTTTCCAATCTCGGCGCCCGCTCGATCGGCTATTGCCTGCTCGCGAT
GGCTGTGGTCAACGGGCTCGACTATCTGTTCGGCACCGTCAGCGAGGCGATGGGCTCGCCGCTGGTGCTGACCGTGATCA
GAAGCCTGTTTGCGGCGCTGATCATCGGCATGATCCTGATCACGGTCTCCTTCGGCAAGCCGATGCTGGCAAGAAACGGC
GATCCCGATGCGCCGGGAAGGCATTGGCCGCGCGGCATGGCGATCATCCTGCGGGTCGTCGGCGCCAGCCTGATCATCGC
CGCGCTGACGGGGTATGTCGGGCTCGCGCGCTTCGTCGCCACGCAGCTCATCATCACCGGTGCGGTTGTCGCTACCATGT
ATGTCGGCCTGCTTTCCGGAAAAGCGATTTCAAGGCAGGAAAGCTTCGGCGACACCTTCTTCGCGCGCTTCCTGACGCGC
CGCTTCAATCTCGGGCCGGTGGCGATCGACCAGGCCGGCCTGCTCGTCGGCCTGGCCATCTATGCCGTGGCGCTGATTAT
CGGCGTCCCGCTGATCCTGCTGCTCTGGGGCTTTCATGTGCAGGATCTGCAGATTATCGCCTATCGGCTGTTTACCGAGG
TCAGGCTCGGCGGCATCAGCATCTCGCTGCTCGGCATCTGCACCGGCATCCTGCTGTTTGCCGGCGTCTACCTGCTGACA
CGCTGGCTGCAGCGCTGGCTCGACGGCAATGTCATGGCAAGAAGCCATGTCGATCTCGGCGTGCGCAATTCCGTCAAGAC
GGGCATCGGCTATCTCGGCGTCGGCATCGCCGCGATCATCGGCGTCTCGGCCGCCGGCATCGATCTCTCGAGCTTCGCAC
TCGTCGCCTCGGCGCTCTCCGTCGGTATCGGTTTCGGCCTGCAGAACATCGTCTCCAACTTCGTCTCCGGCCTGATCCTG
CTCGTCGAACGGCCGTTCAAGGTCGGCGACCACGTCGTGTCGGGCACGGCCGAAGGCATCGTCAAACGCATCTCGGTTCG
TGCCACCGAGATCGAGACTTTCCGCAAGCAGTCGATCATCGTGCCGAATTCGGAGCTGATCAACGGTCTGGTCGGCAACT
GGACGCACCGCAACAAGATCGGCCGCTCGGAAATTCCGGTTTCCGTCAGCTACAATGCCGATCCGCAGCAGGTGATGGAT
ATTCTGCTGGAGCTGACGGCGAAGATACCGCTCGTCATGCGCAATCCCGAGCCGCACGTCGAATTCCTGCGCTTCGGACC
CTATTCGCTTGATTTCGAGCTGCGCTTCTTCCTCGCCGATATGGGCGACGGCATGACGGTGCGCAACAATCTCAGGATCG
AGATCCTCCGGCGCTTCAAGGCCGAAGGTATCGAGATCCCGCTGCCGCAGAGCGACCTCACCATCCATCGCGATGCTCCG
CCTGTCCTTTCCAATGCCGTCAAGGAGCAGACGGACGGCCGAGACGATCCAAAGAACAAGCCCATCGAGGAAGAGGAGCG
GCCGGTGCGGCAGCTGCGCGGGCGGACGGCGGATGGCAGCCTGAAGAGCTGA

Upstream 100 bases:

>100_bases
CGGCTTCTGAACCTTTCCAGGAGCTTCCGCCTTTCTGTCGCCTTTCTTTGCTAGGAAAAGCTGGAACTCCATCCCGACGA
CAACCTTTCCGGAGGCGCTT

Downstream 100 bases:

>100_bases
ACGGCTCATTCAGCCATCATTCACAGGTCTATGTGCATGATCTCCAGCATCGGGATCGCTGCCGAGGGCAGCACCGGTCG
TTCGAGGGGAGGGCGCGTCT

Product: MscS Mechanosensitive ion channel

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 843; Mature: 843

Protein sequence:

>843_residues
MRLRSILLRTILLVTLALAGAHFEGAAAFAQEAQTQAAAPAPVDTPFDQAAKEIDKAKVQLTALQDGVKQNADNDDALVG
LATKADELSRAVITISVNLRPRFDQIKNRLAEIGDPPKDGQPPEAEIVTQERNTLAAERAQINAVTGDAENLSIAVTKLV
NQIIEMRRQLFADTLFKRTEVSASVIDDAAGAFVHEISEFSQALSSWAVFVWKFKRFPMFAAIFLSLASALILLSGSYRL
FGAYLRRDEAVEEPSYIGRLSIAFWSTLIRTMALSVLLVTSFFFLNGFNVLRPDIAPVIGALFGTIGLVYFVGRFANAIF
APREPRWRLVRLSNLGARSIGYCLLAMAVVNGLDYLFGTVSEAMGSPLVLTVIRSLFAALIIGMILITVSFGKPMLARNG
DPDAPGRHWPRGMAIILRVVGASLIIAALTGYVGLARFVATQLIITGAVVATMYVGLLSGKAISRQESFGDTFFARFLTR
RFNLGPVAIDQAGLLVGLAIYAVALIIGVPLILLLWGFHVQDLQIIAYRLFTEVRLGGISISLLGICTGILLFAGVYLLT
RWLQRWLDGNVMARSHVDLGVRNSVKTGIGYLGVGIAAIIGVSAAGIDLSSFALVASALSVGIGFGLQNIVSNFVSGLIL
LVERPFKVGDHVVSGTAEGIVKRISVRATEIETFRKQSIIVPNSELINGLVGNWTHRNKIGRSEIPVSVSYNADPQQVMD
ILLELTAKIPLVMRNPEPHVEFLRFGPYSLDFELRFFLADMGDGMTVRNNLRIEILRRFKAEGIEIPLPQSDLTIHRDAP
PVLSNAVKEQTDGRDDPKNKPIEEEERPVRQLRGRTADGSLKS

Sequences:

>Translated_843_residues
MRLRSILLRTILLVTLALAGAHFEGAAAFAQEAQTQAAAPAPVDTPFDQAAKEIDKAKVQLTALQDGVKQNADNDDALVG
LATKADELSRAVITISVNLRPRFDQIKNRLAEIGDPPKDGQPPEAEIVTQERNTLAAERAQINAVTGDAENLSIAVTKLV
NQIIEMRRQLFADTLFKRTEVSASVIDDAAGAFVHEISEFSQALSSWAVFVWKFKRFPMFAAIFLSLASALILLSGSYRL
FGAYLRRDEAVEEPSYIGRLSIAFWSTLIRTMALSVLLVTSFFFLNGFNVLRPDIAPVIGALFGTIGLVYFVGRFANAIF
APREPRWRLVRLSNLGARSIGYCLLAMAVVNGLDYLFGTVSEAMGSPLVLTVIRSLFAALIIGMILITVSFGKPMLARNG
DPDAPGRHWPRGMAIILRVVGASLIIAALTGYVGLARFVATQLIITGAVVATMYVGLLSGKAISRQESFGDTFFARFLTR
RFNLGPVAIDQAGLLVGLAIYAVALIIGVPLILLLWGFHVQDLQIIAYRLFTEVRLGGISISLLGICTGILLFAGVYLLT
RWLQRWLDGNVMARSHVDLGVRNSVKTGIGYLGVGIAAIIGVSAAGIDLSSFALVASALSVGIGFGLQNIVSNFVSGLIL
LVERPFKVGDHVVSGTAEGIVKRISVRATEIETFRKQSIIVPNSELINGLVGNWTHRNKIGRSEIPVSVSYNADPQQVMD
ILLELTAKIPLVMRNPEPHVEFLRFGPYSLDFELRFFLADMGDGMTVRNNLRIEILRRFKAEGIEIPLPQSDLTIHRDAP
PVLSNAVKEQTDGRDDPKNKPIEEEERPVRQLRGRTADGSLKS
>Mature_843_residues
MRLRSILLRTILLVTLALAGAHFEGAAAFAQEAQTQAAAPAPVDTPFDQAAKEIDKAKVQLTALQDGVKQNADNDDALVG
LATKADELSRAVITISVNLRPRFDQIKNRLAEIGDPPKDGQPPEAEIVTQERNTLAAERAQINAVTGDAENLSIAVTKLV
NQIIEMRRQLFADTLFKRTEVSASVIDDAAGAFVHEISEFSQALSSWAVFVWKFKRFPMFAAIFLSLASALILLSGSYRL
FGAYLRRDEAVEEPSYIGRLSIAFWSTLIRTMALSVLLVTSFFFLNGFNVLRPDIAPVIGALFGTIGLVYFVGRFANAIF
APREPRWRLVRLSNLGARSIGYCLLAMAVVNGLDYLFGTVSEAMGSPLVLTVIRSLFAALIIGMILITVSFGKPMLARNG
DPDAPGRHWPRGMAIILRVVGASLIIAALTGYVGLARFVATQLIITGAVVATMYVGLLSGKAISRQESFGDTFFARFLTR
RFNLGPVAIDQAGLLVGLAIYAVALIIGVPLILLLWGFHVQDLQIIAYRLFTEVRLGGISISLLGICTGILLFAGVYLLT
RWLQRWLDGNVMARSHVDLGVRNSVKTGIGYLGVGIAAIIGVSAAGIDLSSFALVASALSVGIGFGLQNIVSNFVSGLIL
LVERPFKVGDHVVSGTAEGIVKRISVRATEIETFRKQSIIVPNSELINGLVGNWTHRNKIGRSEIPVSVSYNADPQQVMD
ILLELTAKIPLVMRNPEPHVEFLRFGPYSLDFELRFFLADMGDGMTVRNNLRIEILRRFKAEGIEIPLPQSDLTIHRDAP
PVLSNAVKEQTDGRDDPKNKPIEEEERPVRQLRGRTADGSLKS

Specific function: Unknown

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=278, Percent_Identity=33.0935251798561, Blast_Score=149, Evalue=9e-37,
Organism=Escherichia coli, GI2367355, Length=251, Percent_Identity=35.0597609561753, Blast_Score=144, Evalue=3e-35,
Organism=Escherichia coli, GI1789291, Length=227, Percent_Identity=27.3127753303965, Blast_Score=96, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 91826; Mature: 91826

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLRSILLRTILLVTLALAGAHFEGAAAFAQEAQTQAAAPAPVDTPFDQAAKEIDKAKVQ
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LTALQDGVKQNADNDDALVGLATKADELSRAVITISVNLRPRFDQIKNRLAEIGDPPKDG
HHHHHHHHHCCCCCCCCEEEEECCHHHHCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCC
QPPEAEIVTQERNTLAAERAQINAVTGDAENLSIAVTKLVNQIIEMRRQLFADTLFKRTE
CCCCHHEEEHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSASVIDDAAGAFVHEISEFSQALSSWAVFVWKFKRFPMFAAIFLSLASALILLSGSYRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FGAYLRRDEAVEEPSYIGRLSIAFWSTLIRTMALSVLLVTSFFFLNGFNVLRPDIAPVIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHHH
ALFGTIGLVYFVGRFANAIFAPREPRWRLVRLSNLGARSIGYCLLAMAVVNGLDYLFGTV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SEAMGSPLVLTVIRSLFAALIIGMILITVSFGKPMLARNGDPDAPGRHWPRGMAIILRVV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHH
GASLIIAALTGYVGLARFVATQLIITGAVVATMYVGLLSGKAISRQESFGDTFFARFLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RFNLGPVAIDQAGLLVGLAIYAVALIIGVPLILLLWGFHVQDLQIIAYRLFTEVRLGGIS
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
ISLLGICTGILLFAGVYLLTRWLQRWLDGNVMARSHVDLGVRNSVKTGIGYLGVGIAAII
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
GVSAAGIDLSSFALVASALSVGIGFGLQNIVSNFVSGLILLVERPFKVGDHVVSGTAEGI
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCHHHCCHHHHH
VKRISVRATEIETFRKQSIIVPNSELINGLVGNWTHRNKIGRSEIPVSVSYNADPQQVMD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCEEEECCCCHHHHHH
ILLELTAKIPLVMRNPEPHVEFLRFGPYSLDFELRFFLADMGDGMTVRNNLRIEILRRFK
HHHHHHHCCCEEECCCCCCHHHEECCCCEECCEEEEEEECCCCCCEEECCHHHHHHHHHH
AEGIEIPLPQSDLTIHRDAPPVLSNAVKEQTDGRDDPKNKPIEEEERPVRQLRGRTADGS
HCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
LKS
CCC
>Mature Secondary Structure
MRLRSILLRTILLVTLALAGAHFEGAAAFAQEAQTQAAAPAPVDTPFDQAAKEIDKAKVQ
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
LTALQDGVKQNADNDDALVGLATKADELSRAVITISVNLRPRFDQIKNRLAEIGDPPKDG
HHHHHHHHHCCCCCCCCEEEEECCHHHHCCEEEEEEEECCCCHHHHHHHHHHCCCCCCCC
QPPEAEIVTQERNTLAAERAQINAVTGDAENLSIAVTKLVNQIIEMRRQLFADTLFKRTE
CCCCHHEEEHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSASVIDDAAGAFVHEISEFSQALSSWAVFVWKFKRFPMFAAIFLSLASALILLSGSYRL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FGAYLRRDEAVEEPSYIGRLSIAFWSTLIRTMALSVLLVTSFFFLNGFNVLRPDIAPVIG
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHHHHH
ALFGTIGLVYFVGRFANAIFAPREPRWRLVRLSNLGARSIGYCLLAMAVVNGLDYLFGTV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
SEAMGSPLVLTVIRSLFAALIIGMILITVSFGKPMLARNGDPDAPGRHWPRGMAIILRVV
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHH
GASLIIAALTGYVGLARFVATQLIITGAVVATMYVGLLSGKAISRQESFGDTFFARFLTR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RFNLGPVAIDQAGLLVGLAIYAVALIIGVPLILLLWGFHVQDLQIIAYRLFTEVRLGGIS
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCH
ISLLGICTGILLFAGVYLLTRWLQRWLDGNVMARSHVDLGVRNSVKTGIGYLGVGIAAII
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
GVSAAGIDLSSFALVASALSVGIGFGLQNIVSNFVSGLILLVERPFKVGDHVVSGTAEGI
HHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHCCHHHCCHHHHH
VKRISVRATEIETFRKQSIIVPNSELINGLVGNWTHRNKIGRSEIPVSVSYNADPQQVMD
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHCCCCCCCCCEEEECCCCHHHHHH
ILLELTAKIPLVMRNPEPHVEFLRFGPYSLDFELRFFLADMGDGMTVRNNLRIEILRRFK
HHHHHHHCCCEEECCCCCCHHHEECCCCEECCEEEEEEECCCCCCEEECCHHHHHHHHHH
AEGIEIPLPQSDLTIHRDAPPVLSNAVKEQTDGRDDPKNKPIEEEERPVRQLRGRTADGS
HCCCCCCCCCCCCEEECCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCC
LKS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]