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The map label for this gene is greA [H]

Identifier: 209549738

GI number: 209549738

Start: 2205644

End: 2206126

Strand: Reverse

Name: greA [H]

Synonym: Rleg2_2146

Alternate gene names: 209549738

Gene position: 2206126-2205644 (Counterclockwise)

Preceding gene: 209549740

Following gene: 209549737

Centisome position: 48.62

GC content: 63.77

Gene sequence:

>483_bases
TTGGCCGTCGCCTTCACCAAGGAAGAGAGTTTCGAAACTGCTTCGGAAACCCTGCTGCCCGATCGTCCGGTTTCGCCGCA
TCTGAACCTGGTCACGGAGGCTGGGCTGAAGGCTCTGGAATTGCAGTTCCAGCAGGCGCGCGAGGCCTATGATGCGGCGA
GCACCGTCGAAGACGTCAACGAACGGCGGCGGCAGGCGGCAGGTCCCCTGCGCGACCTGCGCTACCTTGCCGCAAGGCTG
CGAACCGCCCAACTCATGGCCGATCCTGCGTCAACCGACACTGTTGCCTTCGGCAGCACGGTGACCTTCAGCCGTGACGA
CGGGCGCGTGCAGACCTATCGGATCGTCGGCGAGGATGAGGCCGATCCCAAGGCCGGATCGATTTCCTATGTCTCGCCGG
TGGCGCGGTTGCTGATGGGCAAGGCTGTCGGCGACGTGGTGGAAACCGGCGGCCAGGAACTGGAGATCATTGCTATCGCG
TAG

Upstream 100 bases:

>100_bases
CCAGATTGGCGGTTCGGCTGCAGCCGGTGTATGTATGGTTTCAAATATGGGCGGTTCGCCCGTCAACTAAAGGGCACTGC
GCCCTAAACGGAGGATAGAA

Downstream 100 bases:

>100_bases
GATGCGATAGTCCAAGCGGTGCCGATTCAGCGAAACGCAATTATTGTTATAAAAACGGTACTGTTTGGTACTTTATTTAT
CTAGCATTTACCAATCGTGA

Product: transcription elongation factor regulatory protein

Products: NA

Alternate protein names: Transcript cleavage factor greA [H]

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MAVAFTKEESFETASETLLPDRPVSPHLNLVTEAGLKALELQFQQAREAYDAASTVEDVNERRRQAAGPLRDLRYLAARL
RTAQLMADPASTDTVAFGSTVTFSRDDGRVQTYRIVGEDEADPKAGSISYVSPVARLLMGKAVGDVVETGGQELEIIAIA

Sequences:

>Translated_160_residues
MAVAFTKEESFETASETLLPDRPVSPHLNLVTEAGLKALELQFQQAREAYDAASTVEDVNERRRQAAGPLRDLRYLAARL
RTAQLMADPASTDTVAFGSTVTFSRDDGRVQTYRIVGEDEADPKAGSISYVSPVARLLMGKAVGDVVETGGQELEIIAIA
>Mature_159_residues
AVAFTKEESFETASETLLPDRPVSPHLNLVTEAGLKALELQFQQAREAYDAASTVEDVNERRRQAAGPLRDLRYLAARLR
TAQLMADPASTDTVAFGSTVTFSRDDGRVQTYRIVGEDEADPKAGSISYVSPVARLLMGKAVGDVVETGGQELEIIAIA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082225, Length=124, Percent_Identity=39.5161290322581, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 17300; Mature: 17168

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAFTKEESFETASETLLPDRPVSPHLNLVTEAGLKALELQFQQAREAYDAASTVEDVN
CCEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRRQAAGPLRDLRYLAARLRTAQLMADPASTDTVAFGSTVTFSRDDGRVQTYRIVGEDE
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCEEEEEEECCCC
ADPKAGSISYVSPVARLLMGKAVGDVVETGGQELEIIAIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC
>Mature Secondary Structure 
AVAFTKEESFETASETLLPDRPVSPHLNLVTEAGLKALELQFQQAREAYDAASTVEDVN
CEECCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ERRRQAAGPLRDLRYLAARLRTAQLMADPASTDTVAFGSTVTFSRDDGRVQTYRIVGEDE
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCEEEEEEECCCC
ADPKAGSISYVSPVARLLMGKAVGDVVETGGQELEIIAIA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA