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The map label for this gene is ddl [H]

Identifier: 209549694

GI number: 209549694

Start: 2162982

End: 2164055

Strand: Direct

Name: ddl [H]

Synonym: Rleg2_2101

Alternate gene names: 209549694

Gene position: 2162982-2164055 (Clockwise)

Preceding gene: 209549693

Following gene: 209549695

Centisome position: 47.66

GC content: 64.9

Gene sequence:

>1074_bases
ATGACCCTTTCGCCAAACAGGCTGCGCATTGCCGTGCTCTTCGGCGGCCGCTCGGCCGAGCATGAGGTTTCCATCCTTTC
GGCGACCAATGTCATGGGTGCGCTCAAGCCCGAGAAATATGACGCCATCCCCGTTTTCATTACCCGCGAGGGACAATGGC
TGCTGAGCAATTTCGAGGACGGCGTATTGGCGCGCCCCTCATCCGGCACGGAAATCTGCATCGTCCCGGGCGGACGGGGA
AGGATGCTGGCAATCCCGGCCCATGGCGCACCGCATGAGCTGGCAAGGATCGACATCCTGTTTCCGGTGTTGCACGGCCT
GCATGGCGAAGACGGATCGGTGCAGGGCCTGGCGGAGGTGGCGCGCGTGCCGCTCGCCGGCTGCGGCATTCTCGGTTCGG
CCGCGGCGCTCGACAAGGATATCGCCAAGCGCCTACTCAGGGCGGCGGGCCTGCCGGTGGCGCGCTCGGTGACGATGCGT
GAGGACGCTGCCCCTTCGCTGGCAGCCCTCGAAAGCGAGCTTGGACTGCCGCTCTTCATCAAGCCGGCGCGTCAGGGATC
GTCGGTCGGCGTCGCCAAGGTCCATGCCAGCCAGGAATTCGCGCCTGCCCTTGCCGAGGCGTTCCGCCACGATCGCATGC
TGCTCGCCGAGGAATTCGTCGCCGGCCGCGAGATCGAATTCAGCGTGTTGGAGGATGCGGCAGGCGAGCTCTTCGTCTCC
CGGCCGGGCGAGATCGTTCCGGCCGAGAGCCACGGCTTCTACAGCTACGATGCCAAATATATCGACGAGAAGGGCGCGGC
GTTGAAGGTCCCGGCCGACCTGCCTGAGGAGGTCGAGGCTGCCATGCGCGACATGGCAGCAAGCGCTTTCCGTGCGGTCG
GATGCGACGGCATGGCCCGCGTCGACTTTTTCCTGACGCAGGACATGCGGTTCCTCGTCAACGAGATCAACACCATCCCC
GGCTTCACCGATATCAGCATGTATTCTAAGGCGATGGCTGCAAGCGGTGTCGGTTACGCCGAAATCATCGACAGGCTGGT
GGAGCACGGCCTGGCGCGCGCGGGCCGGACCTGA

Upstream 100 bases:

>100_bases
TGTAACGCTGCCGCTTGCCGTCTTCGACCGCTTCTTCGCCAATCGCGGCATATCGGTCGACGCCTGACCTTTTCAACACC
AGATCTTTTCGGAGGCTTTC

Downstream 100 bases:

>100_bases
GCATGAACAGATCAGTCCTGATTTCAGGATGTTCCGGCGGCGGAAAATCGACGCTGCTGGCCGAACTTGGCCGTCGCGGT
CATGCGATCGTCGAAGAGCC

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase A; D-alanylalanine synthetaseA [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTLSPNRLRIAVLFGGRSAEHEVSILSATNVMGALKPEKYDAIPVFITREGQWLLSNFEDGVLARPSSGTEICIVPGGRG
RMLAIPAHGAPHELARIDILFPVLHGLHGEDGSVQGLAEVARVPLAGCGILGSAAALDKDIAKRLLRAAGLPVARSVTMR
EDAAPSLAALESELGLPLFIKPARQGSSVGVAKVHASQEFAPALAEAFRHDRMLLAEEFVAGREIEFSVLEDAAGELFVS
RPGEIVPAESHGFYSYDAKYIDEKGAALKVPADLPEEVEAAMRDMAASAFRAVGCDGMARVDFFLTQDMRFLVNEINTIP
GFTDISMYSKAMAASGVGYAEIIDRLVEHGLARAGRT

Sequences:

>Translated_357_residues
MTLSPNRLRIAVLFGGRSAEHEVSILSATNVMGALKPEKYDAIPVFITREGQWLLSNFEDGVLARPSSGTEICIVPGGRG
RMLAIPAHGAPHELARIDILFPVLHGLHGEDGSVQGLAEVARVPLAGCGILGSAAALDKDIAKRLLRAAGLPVARSVTMR
EDAAPSLAALESELGLPLFIKPARQGSSVGVAKVHASQEFAPALAEAFRHDRMLLAEEFVAGREIEFSVLEDAAGELFVS
RPGEIVPAESHGFYSYDAKYIDEKGAALKVPADLPEEVEAAMRDMAASAFRAVGCDGMARVDFFLTQDMRFLVNEINTIP
GFTDISMYSKAMAASGVGYAEIIDRLVEHGLARAGRT
>Mature_356_residues
TLSPNRLRIAVLFGGRSAEHEVSILSATNVMGALKPEKYDAIPVFITREGQWLLSNFEDGVLARPSSGTEICIVPGGRGR
MLAIPAHGAPHELARIDILFPVLHGLHGEDGSVQGLAEVARVPLAGCGILGSAAALDKDIAKRLLRAAGLPVARSVTMRE
DAAPSLAALESELGLPLFIKPARQGSSVGVAKVHASQEFAPALAEAFRHDRMLLAEEFVAGREIEFSVLEDAAGELFVSR
PGEIVPAESHGFYSYDAKYIDEKGAALKVPADLPEEVEAAMRDMAASAFRAVGCDGMARVDFFLTQDMRFLVNEINTIPG
FTDISMYSKAMAASGVGYAEIIDRLVEHGLARAGRT

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=358, Percent_Identity=44.9720670391061, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1786280, Length=349, Percent_Identity=33.2378223495702, Blast_Score=159, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 38061; Mature: 37930

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSPNRLRIAVLFGGRSAEHEVSILSATNVMGALKPEKYDAIPVFITREGQWLLSNFED
CCCCCCEEEEEEEECCCCCCCEEEEEEHHHHHHCCCCCCCCCEEEEEECCCCCEECCCCC
GVLARPSSGTEICIVPGGRGRMLAIPAHGAPHELARIDILFPVLHGLHGEDGSVQGLAEV
CEEECCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ARVPLAGCGILGSAAALDKDIAKRLLRAAGLPVARSVTMREDAAPSLAALESELGLPLFI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCHHHHHHHHCCCCEEE
KPARQGSSVGVAKVHASQEFAPALAEAFRHDRMLLAEEFVAGREIEFSVLEDAAGELFVS
ECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCEEEC
RPGEIVPAESHGFYSYDAKYIDEKGAALKVPADLPEEVEAAMRDMAASAFRAVGCDGMAR
CCCCEEECCCCCCEECCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
VDFFLTQDMRFLVNEINTIPGFTDISMYSKAMAASGVGYAEIIDRLVEHGLARAGRT
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLSPNRLRIAVLFGGRSAEHEVSILSATNVMGALKPEKYDAIPVFITREGQWLLSNFED
CCCCCEEEEEEEECCCCCCCEEEEEEHHHHHHCCCCCCCCCEEEEEECCCCCEECCCCC
GVLARPSSGTEICIVPGGRGRMLAIPAHGAPHELARIDILFPVLHGLHGEDGSVQGLAEV
CEEECCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
ARVPLAGCGILGSAAALDKDIAKRLLRAAGLPVARSVTMREDAAPSLAALESELGLPLFI
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEECCCCCCCHHHHHHHHCCCCEEE
KPARQGSSVGVAKVHASQEFAPALAEAFRHDRMLLAEEFVAGREIEFSVLEDAAGELFVS
ECCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCCCCEEEC
RPGEIVPAESHGFYSYDAKYIDEKGAALKVPADLPEEVEAAMRDMAASAFRAVGCDGMAR
CCCCEEECCCCCCEECCCHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
VDFFLTQDMRFLVNEINTIPGFTDISMYSKAMAASGVGYAEIIDRLVEHGLARAGRT
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]