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The map label for this gene is garR [C]

Identifier: 209549665

GI number: 209549665

Start: 2132249

End: 2133148

Strand: Direct

Name: garR [C]

Synonym: Rleg2_2072

Alternate gene names: 209549665

Gene position: 2132249-2133148 (Clockwise)

Preceding gene: 209549657

Following gene: 209549666

Centisome position: 46.99

GC content: 61.78

Gene sequence:

>900_bases
ATGGCTAAAGTCGCATTCATCGGTCTCGGCGTCATGGGTTTTCCCATGGCCGGTCATCTGAAGACGAAGGGCGGCCACGA
TGTCACCGTCTACAATCGCACAGTCGAAAAAGCCGTCGCCTGGGCTGAAAAATTCTCCGGCAAATCCGCATCCACCCCGG
CCGAGGCTGCCGCGGGCGCCGATTTCGTCTTCGTCTGCGTCGGCAATGACGAAGATCTCCGCTCGGTGACATCGGGCGAA
AACGGCGTCCTGCACGGCATGAAGCCGGGGTCGGTGCTGATCGACAACACCACCGCCAGCGCCGAGGTCGCGCGCGAACT
TTATGCCGCTGCCAAGGAAAAAGGCGTCGATTTCATCGACGCTCCCGTTTCCGGCGGCCAGGCCGGCGCTGAAAACGGCG
TCCTGACCGTGATGTGCGGCGGCGACGAGGCCGTCTTCGATCGTGCCAGGCCCGTCATCGACGCCTATGCCCGCATGGTC
GGTCTGATGGGACCTGCAGGCTCTGGCCAGTTGACCAAGATGGTCAACCAGATCTGCATCGCCGGCCTCGTCCAGGGGCT
TGCCGAAGGATTGCATTTCGGCAAGCGCGCCGGCCTTGATATCGAAAAGGTCGTCGAGGTGATCTCCAAGGGGGCGGCAG
GCTCCTGGCAGATGGAAAACCGCCACAAGACCATGAACGCCGGCAAGTATGATTTCGGCTTCGCGGTCGACTGGATGCGC
AAGGATCTCGGTATCGTGCTCACCGAAGCCCGCCGCAACGGCGCCAAGCTGCCGGTCACCGCGGTCGTCGACCAGTTCTA
CGGCGACGTCCAGGCGATGGGCGGCAATCGCTGGGATACATCTTCGTTGCTTGCGCGTCTGGAACAGGAAGATTTTAGGC
CGGATCGGCCTAAAATCTGA

Upstream 100 bases:

>100_bases
CATATCCGTCTATCGCGAGGAACTCCGTCTTCACGGCTTTTCTGCTCCGGCCGTTTCGGTTAATCCTCAGGGCCTCGCAC
TCGGGATAAGGACAGGACAT

Downstream 100 bases:

>100_bases
ATCCTGTTCCAAACTAAGAGGAAGAGCATGATGTCGTCCGAAAACCGCTCACACTTTTCGGCATCATGCTCTGGAAAAAT
GATCGACCTCTCCTCCAGCG

Product: NAD-binding 6-phosphogluconate dehydrogenase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MAKVAFIGLGVMGFPMAGHLKTKGGHDVTVYNRTVEKAVAWAEKFSGKSASTPAEAAAGADFVFVCVGNDEDLRSVTSGE
NGVLHGMKPGSVLIDNTTASAEVARELYAAAKEKGVDFIDAPVSGGQAGAENGVLTVMCGGDEAVFDRARPVIDAYARMV
GLMGPAGSGQLTKMVNQICIAGLVQGLAEGLHFGKRAGLDIEKVVEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
KDLGIVLTEARRNGAKLPVTAVVDQFYGDVQAMGGNRWDTSSLLARLEQEDFRPDRPKI

Sequences:

>Translated_299_residues
MAKVAFIGLGVMGFPMAGHLKTKGGHDVTVYNRTVEKAVAWAEKFSGKSASTPAEAAAGADFVFVCVGNDEDLRSVTSGE
NGVLHGMKPGSVLIDNTTASAEVARELYAAAKEKGVDFIDAPVSGGQAGAENGVLTVMCGGDEAVFDRARPVIDAYARMV
GLMGPAGSGQLTKMVNQICIAGLVQGLAEGLHFGKRAGLDIEKVVEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
KDLGIVLTEARRNGAKLPVTAVVDQFYGDVQAMGGNRWDTSSLLARLEQEDFRPDRPKI
>Mature_298_residues
AKVAFIGLGVMGFPMAGHLKTKGGHDVTVYNRTVEKAVAWAEKFSGKSASTPAEAAAGADFVFVCVGNDEDLRSVTSGEN
GVLHGMKPGSVLIDNTTASAEVARELYAAAKEKGVDFIDAPVSGGQAGAENGVLTVMCGGDEAVFDRARPVIDAYARMVG
LMGPAGSGQLTKMVNQICIAGLVQGLAEGLHFGKRAGLDIEKVVEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMRK
DLGIVLTEARRNGAKLPVTAVVDQFYGDVQAMGGNRWDTSSLLARLEQEDFRPDRPKI

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=295, Percent_Identity=31.1864406779661, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI40556376, Length=281, Percent_Identity=25.6227758007117, Blast_Score=129, Evalue=3e-30,
Organism=Escherichia coli, GI145693186, Length=287, Percent_Identity=36.2369337979094, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1786719, Length=286, Percent_Identity=32.8671328671329, Blast_Score=160, Evalue=8e-41,
Organism=Escherichia coli, GI1790315, Length=291, Percent_Identity=31.2714776632302, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1789092, Length=226, Percent_Identity=32.7433628318584, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17557316, Length=276, Percent_Identity=28.9855072463768, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24655230, Length=297, Percent_Identity=28.2828282828283, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI19922568, Length=297, Percent_Identity=28.2828282828283, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574115, Length=283, Percent_Identity=24.0282685512367, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- InterPro:   IPR006183 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 31656; Mature: 31525

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVAFIGLGVMGFPMAGHLKTKGGHDVTVYNRTVEKAVAWAEKFSGKSASTPAEAAAGA
CCEEEEEECCHHHCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
DFVFVCVGNDEDLRSVTSGENGVLHGMKPGSVLIDNTTASAEVARELYAAAKEKGVDFID
CEEEEEECCCHHHHHHHCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEE
APVSGGQAGAENGVLTVMCGGDEAVFDRARPVIDAYARMVGLMGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AGLVQGLAEGLHFGKRAGLDIEKVVEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCHHHCCCCCCCCCHHHHHHH
KDLGIVLTEARRNGAKLPVTAVVDQFYGDVQAMGGNRWDTSSLLARLEQEDFRPDRPKI
HCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AKVAFIGLGVMGFPMAGHLKTKGGHDVTVYNRTVEKAVAWAEKFSGKSASTPAEAAAGA
CEEEEEECCHHHCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
DFVFVCVGNDEDLRSVTSGENGVLHGMKPGSVLIDNTTASAEVARELYAAAKEKGVDFID
CEEEEEECCCHHHHHHHCCCCCEEECCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEE
APVSGGQAGAENGVLTVMCGGDEAVFDRARPVIDAYARMVGLMGPAGSGQLTKMVNQICI
CCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
AGLVQGLAEGLHFGKRAGLDIEKVVEVISKGAAGSWQMENRHKTMNAGKYDFGFAVDWMR
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCEECCCHHHCCCCCCCCCHHHHHHH
KDLGIVLTEARRNGAKLPVTAVVDQFYGDVQAMGGNRWDTSSLLARLEQEDFRPDRPKI
HCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA