Click here to switch to the map view.

The map label for this gene is rnhA [H]

Identifier: 209549587

GI number: 209549587

Start: 2051312

End: 2051845

Strand: Reverse

Name: rnhA [H]

Synonym: Rleg2_1994

Alternate gene names: 209549587

Gene position: 2051845-2051312 (Counterclockwise)

Preceding gene: 209549589

Following gene: 209549585

Centisome position: 45.22

GC content: 59.36

Gene sequence:

>534_bases
ATGACAGGCATTCCCGATGACATCCGACCCTCCGCACCGGCATCAGGCGTACCAGAATCCCAACTGGGGCTGCACCTCTT
CGCGGACGGTTGTTATGAGCCCGGTTCCGGGCATGGAGGCTGGTCGTTTGTCGCTTATCGCGATGCGGTGGAAATCGCCT
CCGGCTTCGGTGGCGCTGAAGATTCCAGCAATAATTCCATGGAATTGACCGCCGTTCTCAGGGCAGCCATGTGGATCAAT
AGCCAAACAGCGGGCGAAGCCGCGATCATCTGGTCCGATTCCATCTATGCCGTCAAAGGCTGCAACAGCCGGCGACATAT
CTGGAAGAATAACGGCTGGAAGAAGAGCAGCCCGAATGGACAGGGCCGAAGGCGAACGATCGACAATGCGGAGCTCTGGA
AAGCGGTCGATCTTCAGCTATCACAAAACGCCCTGGTGACCATTGCCTGGTGCAAGGGGCATTCGGGGGTTGTCGGCAAC
GAGCGCGCGGACGCGCTCGCCGATAGAGGACGGCTGTCGATAGCCGGCGGCTGA

Upstream 100 bases:

>100_bases
CCGCGCGCTTCCTCTTAATCTATTTCTCTTAATAAGCTCAAAGAAAATCCTAGCGTATTTTCGCGATCTTATCCAGCAAT
CGCCCGAGACCCCGGTAAAA

Downstream 100 bases:

>100_bases
TACGGTGCTGGCCGGTTTCAGGCGCATGTCGTTGCTCGGCCGGCAACCGGCTTACGCCGGCTGCGATGCCTCGACCACGG
CGAGGGCGGCCATGTTGACG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MTGIPDDIRPSAPASGVPESQLGLHLFADGCYEPGSGHGGWSFVAYRDAVEIASGFGGAEDSSNNSMELTAVLRAAMWIN
SQTAGEAAIIWSDSIYAVKGCNSRRHIWKNNGWKKSSPNGQGRRRTIDNAELWKAVDLQLSQNALVTIAWCKGHSGVVGN
ERADALADRGRLSIAGG

Sequences:

>Translated_177_residues
MTGIPDDIRPSAPASGVPESQLGLHLFADGCYEPGSGHGGWSFVAYRDAVEIASGFGGAEDSSNNSMELTAVLRAAMWIN
SQTAGEAAIIWSDSIYAVKGCNSRRHIWKNNGWKKSSPNGQGRRRTIDNAELWKAVDLQLSQNALVTIAWCKGHSGVVGN
ERADALADRGRLSIAGG
>Mature_176_residues
TGIPDDIRPSAPASGVPESQLGLHLFADGCYEPGSGHGGWSFVAYRDAVEIASGFGGAEDSSNNSMELTAVLRAAMWINS
QTAGEAAIIWSDSIYAVKGCNSRRHIWKNNGWKKSSPNGQGRRRTIDNAELWKAVDLQLSQNALVTIAWCKGHSGVVGNE
RADALADRGRLSIAGG

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Escherichia coli, GI1786408, Length=145, Percent_Identity=31.0344827586207, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 18813; Mature: 18682

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGIPDDIRPSAPASGVPESQLGLHLFADGCYEPGSGHGGWSFVAYRDAVEIASGFGGAE
CCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCC
DSSNNSMELTAVLRAAMWINSQTAGEAAIIWSDSIYAVKGCNSRRHIWKNNGWKKSSPNG
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCEECCCCCCCCCCCC
QGRRRTIDNAELWKAVDLQLSQNALVTIAWCKGHSGVVGNERADALADRGRLSIAGG
CCCEEECCCHHHHEEECEEECCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEECCC
>Mature Secondary Structure 
TGIPDDIRPSAPASGVPESQLGLHLFADGCYEPGSGHGGWSFVAYRDAVEIASGFGGAE
CCCCCCCCCCCCCCCCCHHHCCEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHCCCCCC
DSSNNSMELTAVLRAAMWINSQTAGEAAIIWSDSIYAVKGCNSRRHIWKNNGWKKSSPNG
CCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCEECCCCCCCCCCCC
QGRRRTIDNAELWKAVDLQLSQNALVTIAWCKGHSGVVGNERADALADRGRLSIAGG
CCCEEECCCHHHHEEECEEECCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA