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The map label for this gene is dme [H]

Identifier: 209549586

GI number: 209549586

Start: 2048948

End: 2051260

Strand: Direct

Name: dme [H]

Synonym: Rleg2_1993

Alternate gene names: 209549586

Gene position: 2048948-2051260 (Clockwise)

Preceding gene: 209549584

Following gene: 209549588

Centisome position: 45.15

GC content: 64.72

Gene sequence:

>2313_bases
ATGGATCACCAGGAAAAATCCAAGACGGAAAAGAACCTGACAAGCGGCGATCTCGACGAGCAGGCGCTGTTCTTCCACCG
CTATCCCCGCCCCGGCAAGCTGGAAATCCAGGCAACCAAGCCGCTCGGCAACCAGCGCGATCTGGCGCTCGCCTATTCGC
CCGGCGTCGCCGCCCCTTGCCTTGCCATCCGCGACAATCCTGAGATGGCGGCCGAATATACCTCGCGCGCCAATCTCGTC
GCCGTCATCTCGAACGGCACCGCCGTGCTCGGCCTCGGCAATATCGGCCCGCTGGCCTCGAAGCCGGTGATGGAGGGCAA
GGCCGTGCTCTTCAAGAAATTCGCCGGCATCGATGTCTTCGATATCGAAATCGACGCGGCAAGCGTCGAGCAGATGGTCT
CGACCGTCTCCTCGCTGGAGCCGACCTTCGGCGGCATCAACCTCGAGGACATCAAGGCGCCCGAATGCTTCGAAGTCGAG
CGGCGCCTGCGCGAAAAGATGAAGATCCCGGTCTTCCACGACGACCAGCACGGCACGGCGATCATCGTCGCCGCCGCGAT
CCTGAACGGACTGGAACTCGCCGGCAAGGCGATCGAGAACGTTAAGATCGTCGCCTCAGGCGCCGGGGCCGCCGCCCTTG
CCTGCCTCAACCTGCTTGTCATTCTCGGTGCAAAACGCGAAAACATCTGGGTCCACGATCTCGAAGGCCTCGTCTATGAG
GGCCGCACCGAGCTGATGGACGAATGGAAATCCGTCTATGCCCAGAAGAGCGACACGCGCACGCTCGCCGAAAATATCGG
CGGCGCCGACGTCTTCCTCGGCCTGTCAGCCGCCGGCGTACTCAAGCCCGAACTGCTGGCGCAGATGGCCGACAAGCCGC
TGATCATGGCGCTCGCCAATCCGACGCCCGAAATCATGCCCGATCTCGCACGTGCCGCCCGCCCCGACGCGATGATCTGC
ACCGGCCGCTCGGATTTCGCCAACCAGGTCAACAACGTCCTCTGCTTCCCCTATATCTTCCGCGGCGCGCTAGATTGCGG
CGCCGAGACGATCAACGAGGAAATGAAGATGGCGGCCGTGCGCGCCATCGCCGCCCTTGCCCGCGAAGAGCCGTCCGATG
TCGCCGCCCGCGCCTATTCCGGCGAAACCCCGGTCTTCGGCCCGGATTACCTGATCCCCTCGCCCTTCGATCCGCGCCTC
ATCCTGCGCATCGCGCCTGCAGTCGCCAAGGCCGCCGAACAGAGCGGCGTGGCGCGCCGCCCGATCCAGGATTTCGACGC
CTATCTCGATCAGTTGAACCGCTTCGTCTTCCGCTCCGGCTTCGTCATGAAGCCGATCTTCACCGCGGCCAAGGCCGCCG
AGCGTAAGCGCGTCATCTTCTCCGAAGGCGAGGACGAACGCGTGCTGCGCGCCGCCCAGGTACTGCTTGAGGAAGGCCTT
GCCGATCCCATCCTGATTGGCCGCCCGCAGGTCATCGAGACGCGCCTGAAGCGCTACGGCCTGCGCATCCGGCCGCTGCA
GGATTTCGAGGTCATCAATCCGGAAGACGATCCGCGCTTCCGCGAATATGTCGATCTCTATTTCTCCCTCGTCGGCCGCC
GCGGCGTCATCCCGGAGGCCGCCCGCACCATCGTGCGTACCAATACCACCGTCATCGGCGCGCTGGCGCTGAGGCGCGGC
GAGGCCGATGCGCTGATCTGCGGTCTGGAAGGCCGCTATGAAAAGCATCTGCGCGATGTCCGCCAGATCATCGGCAAGCG
CAAGAATGTCCGCGATTTCTCAGCCCTCAGCCTGATGATCTCGCAGCGCGGCGCCACCTTCTTCACCGACACCTACGTCA
CCTTCAATCCCAGCGCCGAGGAAGTTGCCGAGGCAACGGTGCTGGCGGCCGAAGAAATCCGCCGTTTCGGCATCACCCCG
CGCGCTGCCCTCGTCTCGCACTCCAACTTCGGCTCTCGCGAATCCGAAAGCGCCACGAAGATGCGCAACGCCCTGCAGCT
GGTGCGCGAGACCGCCCCAGATCTCGAGGTCGACGGCGAAATGCATGGCGAAAGCGCCATCACCGAGGCACTGCGCAAGC
GCGTCATGCCGGATACGACGCTGCACGACGAGGCGAACCTGCTGGTCTTCCCGAACCTCGACGCCGCCAACATCACGCTC
GGCGTCGTCAAGTCGATGACCGACGGCCTGCATGTCGGCCCGATCCTGCTCGGCACCGCCCTGCCCGCCCACATCCTGGC
GCCTTCGGTCACCTCCCGCGGCGTCGTCAACATGGCCGCCCTCGCCGTGGTCGAGGCATCGCAGCCGGCGTAA

Upstream 100 bases:

>100_bases
CCGCCTCGCGAAAACTTCTCCCATCCCCCATTTCCATACTGTCGCCCGTATCCGATTGATGTATGGAGGAACTTCAAAAA
AGACGTGCACGGAGGCGGCA

Downstream 100 bases:

>100_bases
GCCGGTTGCCGGCCGAGCAACGACATGCGCCTGAAACCGGCCAGCACCGTATCAGCCGCCGGCTATCGACAGCCGTCCTC
TATCGGCGAGCGCGTCCGCG

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MDHQEKSKTEKNLTSGDLDEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIRDNPEMAAEYTSRANLV
AVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAASVEQMVSTVSSLEPTFGGINLEDIKAPECFEVE
RRLREKMKIPVFHDDQHGTAIIVAAAILNGLELAGKAIENVKIVASGAGAAALACLNLLVILGAKRENIWVHDLEGLVYE
GRTELMDEWKSVYAQKSDTRTLAENIGGADVFLGLSAAGVLKPELLAQMADKPLIMALANPTPEIMPDLARAARPDAMIC
TGRSDFANQVNNVLCFPYIFRGALDCGAETINEEMKMAAVRAIAALAREEPSDVAARAYSGETPVFGPDYLIPSPFDPRL
ILRIAPAVAKAAEQSGVARRPIQDFDAYLDQLNRFVFRSGFVMKPIFTAAKAAERKRVIFSEGEDERVLRAAQVLLEEGL
ADPILIGRPQVIETRLKRYGLRIRPLQDFEVINPEDDPRFREYVDLYFSLVGRRGVIPEAARTIVRTNTTVIGALALRRG
EADALICGLEGRYEKHLRDVRQIIGKRKNVRDFSALSLMISQRGATFFTDTYVTFNPSAEEVAEATVLAAEEIRRFGITP
RAALVSHSNFGSRESESATKMRNALQLVRETAPDLEVDGEMHGESAITEALRKRVMPDTTLHDEANLLVFPNLDAANITL
GVVKSMTDGLHVGPILLGTALPAHILAPSVTSRGVVNMAALAVVEASQPA

Sequences:

>Translated_770_residues
MDHQEKSKTEKNLTSGDLDEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIRDNPEMAAEYTSRANLV
AVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAASVEQMVSTVSSLEPTFGGINLEDIKAPECFEVE
RRLREKMKIPVFHDDQHGTAIIVAAAILNGLELAGKAIENVKIVASGAGAAALACLNLLVILGAKRENIWVHDLEGLVYE
GRTELMDEWKSVYAQKSDTRTLAENIGGADVFLGLSAAGVLKPELLAQMADKPLIMALANPTPEIMPDLARAARPDAMIC
TGRSDFANQVNNVLCFPYIFRGALDCGAETINEEMKMAAVRAIAALAREEPSDVAARAYSGETPVFGPDYLIPSPFDPRL
ILRIAPAVAKAAEQSGVARRPIQDFDAYLDQLNRFVFRSGFVMKPIFTAAKAAERKRVIFSEGEDERVLRAAQVLLEEGL
ADPILIGRPQVIETRLKRYGLRIRPLQDFEVINPEDDPRFREYVDLYFSLVGRRGVIPEAARTIVRTNTTVIGALALRRG
EADALICGLEGRYEKHLRDVRQIIGKRKNVRDFSALSLMISQRGATFFTDTYVTFNPSAEEVAEATVLAAEEIRRFGITP
RAALVSHSNFGSRESESATKMRNALQLVRETAPDLEVDGEMHGESAITEALRKRVMPDTTLHDEANLLVFPNLDAANITL
GVVKSMTDGLHVGPILLGTALPAHILAPSVTSRGVVNMAALAVVEASQPA
>Mature_770_residues
MDHQEKSKTEKNLTSGDLDEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPCLAIRDNPEMAAEYTSRANLV
AVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAASVEQMVSTVSSLEPTFGGINLEDIKAPECFEVE
RRLREKMKIPVFHDDQHGTAIIVAAAILNGLELAGKAIENVKIVASGAGAAALACLNLLVILGAKRENIWVHDLEGLVYE
GRTELMDEWKSVYAQKSDTRTLAENIGGADVFLGLSAAGVLKPELLAQMADKPLIMALANPTPEIMPDLARAARPDAMIC
TGRSDFANQVNNVLCFPYIFRGALDCGAETINEEMKMAAVRAIAALAREEPSDVAARAYSGETPVFGPDYLIPSPFDPRL
ILRIAPAVAKAAEQSGVARRPIQDFDAYLDQLNRFVFRSGFVMKPIFTAAKAAERKRVIFSEGEDERVLRAAQVLLEEGL
ADPILIGRPQVIETRLKRYGLRIRPLQDFEVINPEDDPRFREYVDLYFSLVGRRGVIPEAARTIVRTNTTVIGALALRRG
EADALICGLEGRYEKHLRDVRQIIGKRKNVRDFSALSLMISQRGATFFTDTYVTFNPSAEEVAEATVLAAEEIRRFGITP
RAALVSHSNFGSRESESATKMRNALQLVRETAPDLEVDGEMHGESAITEALRKRVMPDTTLHDEANLLVFPNLDAANITL
GVVKSMTDGLHVGPILLGTALPAHILAPSVTSRGVVNMAALAVVEASQPA

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=355, Percent_Identity=24.7887323943662, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI62420882, Length=407, Percent_Identity=23.8329238329238, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI62420880, Length=407, Percent_Identity=23.8329238329238, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI239049447, Length=407, Percent_Identity=23.8329238329238, Blast_Score=76, Evalue=1e-13,
Organism=Homo sapiens, GI4505143, Length=345, Percent_Identity=22.6086956521739, Blast_Score=74, Evalue=7e-13,
Organism=Homo sapiens, GI270265879, Length=322, Percent_Identity=25.776397515528, Blast_Score=72, Evalue=3e-12,
Organism=Escherichia coli, GI1788806, Length=755, Percent_Identity=59.3377483443709, Blast_Score=909, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=312, Percent_Identity=31.0897435897436, Blast_Score=139, Evalue=8e-34,
Organism=Escherichia coli, GI1788635, Length=314, Percent_Identity=27.7070063694268, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI87081919, Length=294, Percent_Identity=26.530612244898, Blast_Score=66, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17537199, Length=416, Percent_Identity=23.5576923076923, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=272, Percent_Identity=28.3088235294118, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281362672, Length=404, Percent_Identity=23.019801980198, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356279, Length=404, Percent_Identity=23.019801980198, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI281362674, Length=404, Percent_Identity=23.019801980198, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646388, Length=349, Percent_Identity=26.3610315186246, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24646386, Length=349, Percent_Identity=26.3610315186246, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83751; Mature: 83751

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHQEKSKTEKNLTSGDLDEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPC
CCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCE
LAIRDNPEMAAEYTSRANLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEIDAASVEQMVSTVSSLEPTFGGINLEDIKAPECFEVERRLREKMKIPVFHDDQHGTA
EEEECHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCCH
IIVAAAILNGLELAGKAIENVKIVASGAGAAALACLNLLVILGAKRENIWVHDLEGLVYE
HHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHCC
GRTELMDEWKSVYAQKSDTRTLAENIGGADVFLGLSAAGVLKPELLAQMADKPLIMALAN
CHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECC
PTPEIMPDLARAARPDAMICTGRSDFANQVNNVLCFPYIFRGALDCGAETINEEMKMAAV
CCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHH
RAIAALAREEPSDVAARAYSGETPVFGPDYLIPSPFDPRLILRIAPAVAKAAEQSGVARR
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHCCCCCC
PIQDFDAYLDQLNRFVFRSGFVMKPIFTAAKAAERKRVIFSEGEDERVLRAAQVLLEEGL
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCC
ADPILIGRPQVIETRLKRYGLRIRPLQDFEVINPEDDPRFREYVDLYFSLVGRRGVIPEA
CCCEEECCCHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
ARTIVRTNTTVIGALALRRGEADALICGLEGRYEKHLRDVRQIIGKRKNVRDFSALSLMI
HHHHHHCCCHHEEHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SQRGATFFTDTYVTFNPSAEEVAEATVLAAEEIRRFGITPRAALVSHSNFGSRESESATK
HHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCHHHHHH
MRNALQLVRETAPDLEVDGEMHGESAITEALRKRVMPDTTLHDEANLLVFPNLDAANITL
HHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHH
GVVKSMTDGLHVGPILLGTALPAHILAPSVTSRGVVNMAALAVVEASQPA
HHHHHHCCCCEECHHHHHHHCHHHHHCCCCCCCCHHHHHHHHEEECCCCC
>Mature Secondary Structure
MDHQEKSKTEKNLTSGDLDEQALFFHRYPRPGKLEIQATKPLGNQRDLALAYSPGVAAPC
CCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCE
LAIRDNPEMAAEYTSRANLVAVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCHHHHHHHHCCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEIDAASVEQMVSTVSSLEPTFGGINLEDIKAPECFEVERRLREKMKIPVFHDDQHGTA
EEEECHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCCEECCCCCCH
IIVAAAILNGLELAGKAIENVKIVASGAGAAALACLNLLVILGAKRENIWVHDLEGLVYE
HHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHCC
GRTELMDEWKSVYAQKSDTRTLAENIGGADVFLGLSAAGVLKPELLAQMADKPLIMALAN
CHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHCCCCEEEEECC
PTPEIMPDLARAARPDAMICTGRSDFANQVNNVLCFPYIFRGALDCGAETINEEMKMAAV
CCHHHHHHHHHHCCCCCEEEECCHHHHHHHCCEEEHHHHHHHHHHHCHHHHHHHHHHHHH
RAIAALAREEPSDVAARAYSGETPVFGPDYLIPSPFDPRLILRIAPAVAKAAEQSGVARR
HHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHEEHHHHHHHHHHHCCCCCC
PIQDFDAYLDQLNRFVFRSGFVMKPIFTAAKAAERKRVIFSEGEDERVLRAAQVLLEEGL
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHCC
ADPILIGRPQVIETRLKRYGLRIRPLQDFEVINPEDDPRFREYVDLYFSLVGRRGVIPEA
CCCEEECCCHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHH
ARTIVRTNTTVIGALALRRGEADALICGLEGRYEKHLRDVRQIIGKRKNVRDFSALSLMI
HHHHHHCCCHHEEHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SQRGATFFTDTYVTFNPSAEEVAEATVLAAEEIRRFGITPRAALVSHSNFGSRESESATK
HHCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCHHHHHH
MRNALQLVRETAPDLEVDGEMHGESAITEALRKRVMPDTTLHDEANLLVFPNLDAANITL
HHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHH
GVVKSMTDGLHVGPILLGTALPAHILAPSVTSRGVVNMAALAVVEASQPA
HHHHHHCCCCEECHHHHHHHCHHHHHCCCCCCCCHHHHHHHHEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]