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The map label for this gene is mdtE [H]

Identifier: 209549583

GI number: 209549583

Start: 2045213

End: 2046391

Strand: Reverse

Name: mdtE [H]

Synonym: Rleg2_1990

Alternate gene names: 209549583

Gene position: 2046391-2045213 (Counterclockwise)

Preceding gene: 209549585

Following gene: 209549581

Centisome position: 45.1

GC content: 65.22

Gene sequence:

>1179_bases
ATGAACATGCTTTTTCATACGAAATCATTGCGTTGCGCGCTAATGCTGGCGCTGCTGCTCTTCGCCGGCCTGCCGGCTTA
CGCGCAGGAAGGCGGCGCCATGCCGCCCGCTGCCGTCAGCGTGCTGACGCTCGACGCGCGTCCGGTTCCCGTTGTCAGCG
AATTGCCCGGGCGCATTGCCGCGACGCGGGTTTCGGAGGTGCGGGCCAGGGTTTCCGGCATCCTGCAGGAACGGGTCTTC
GAGCAGGGCAGTCTGGTTCAGCAGGGCGACGTGCTCTATCGGATCGATCCGGCTCTGTTTCGGGTTCGCGTCGCAAGCGC
CGAAGCAAGCCTCGAGCGTGCCAAGGCGACGCAGTTGAATGCCCGCCAGCAGCTGGAGCGCCAGAAATCGCTGCGTGACC
GCGATGTCGCAAGCGGGGTCGAATATGATGCGGCAGCCGTCGCCCTTGCCCAGGCCGATGCCGATGTCGCGCTGGCGCAG
GCCGCACTCGACGAAGCGAAAATCAATCTCGGCTATACCGAAGTCCGGGCACCGATATCAGGCATCATCGGCGGTGCGCT
GGTCACCGAGGGCGCGCTGGTGACGGCTGATGCCGGCGATGCGCTGGCGATGATCCAGCAGATCGACCCCGTCTATGCCG
ATTTCACGCAATCCTCCGGCGACTTGCTGGCGCTGAAGCGGGCGGTCGAGAACGGCAGCCTCGCCTCGACGGAAGCCGGG
AAGGCCGACATCAAGCTGGTCTTCGACGACGGCACGGTCTATGGCCAATCCGGAAAGCTGCTATTCCGCAGCGCCAGTGT
CGATACGACCACCGGCCAGGTCACACTGCGCGGCGAATTCCCCAATCCCAAAGGCGATCTGCTGCCGGGTCTCTACGTCA
GGGTCCGCATCGAGCAGGCCGTCCGCGAGAAGGCGATCCTCATCCCGCAACGCGCCGTTATCCGCGCGGCCGATGGCAAG
GCGCAGGTCTATGTCGTCGAGCAGGGAGACGTGGCGCAACCGCGCGATGTCGAGCTCAGTCAATCCTCCGGCAATGAATG
GGTGGTCGAAAGCGGCTTGTCCGCAGGCGAACGCCTGGTCGTCGACGGCGTGCAGAAACTGCAGCCGGGCGCCAAGGTCG
CGCCTGAGGAGTGGCGCAGTGGCGAAGTCGCGTCCGGTGACGCCAAGGAGCCGGAGTAG

Upstream 100 bases:

>100_bases
GCGAAAAAAGATCGACTATCTCAGGGCTCAGGAGGCCCGCCGAACGTCATTTCGGCGGGCTGTGCTTTTTTGGACCTCAG
CGGGACCATCAGGAAGGCTT

Downstream 100 bases:

>100_bases
GATCGATGGCACGTTTCTTTATCGATCGCCCGATCTTGGCCTGGGTCTTTGCGATCTTCATCTCCATCGCCGGCCTGATC
GCGCTGCCGTTCCTGCCGGT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MNMLFHTKSLRCALMLALLLFAGLPAYAQEGGAMPPAAVSVLTLDARPVPVVSELPGRIAATRVSEVRARVSGILQERVF
EQGSLVQQGDVLYRIDPALFRVRVASAEASLERAKATQLNARQQLERQKSLRDRDVASGVEYDAAAVALAQADADVALAQ
AALDEAKINLGYTEVRAPISGIIGGALVTEGALVTADAGDALAMIQQIDPVYADFTQSSGDLLALKRAVENGSLASTEAG
KADIKLVFDDGTVYGQSGKLLFRSASVDTTTGQVTLRGEFPNPKGDLLPGLYVRVRIEQAVREKAILIPQRAVIRAADGK
AQVYVVEQGDVAQPRDVELSQSSGNEWVVESGLSAGERLVVDGVQKLQPGAKVAPEEWRSGEVASGDAKEPE

Sequences:

>Translated_392_residues
MNMLFHTKSLRCALMLALLLFAGLPAYAQEGGAMPPAAVSVLTLDARPVPVVSELPGRIAATRVSEVRARVSGILQERVF
EQGSLVQQGDVLYRIDPALFRVRVASAEASLERAKATQLNARQQLERQKSLRDRDVASGVEYDAAAVALAQADADVALAQ
AALDEAKINLGYTEVRAPISGIIGGALVTEGALVTADAGDALAMIQQIDPVYADFTQSSGDLLALKRAVENGSLASTEAG
KADIKLVFDDGTVYGQSGKLLFRSASVDTTTGQVTLRGEFPNPKGDLLPGLYVRVRIEQAVREKAILIPQRAVIRAADGK
AQVYVVEQGDVAQPRDVELSQSSGNEWVVESGLSAGERLVVDGVQKLQPGAKVAPEEWRSGEVASGDAKEPE
>Mature_392_residues
MNMLFHTKSLRCALMLALLLFAGLPAYAQEGGAMPPAAVSVLTLDARPVPVVSELPGRIAATRVSEVRARVSGILQERVF
EQGSLVQQGDVLYRIDPALFRVRVASAEASLERAKATQLNARQQLERQKSLRDRDVASGVEYDAAAVALAQADADVALAQ
AALDEAKINLGYTEVRAPISGIIGGALVTEGALVTADAGDALAMIQQIDPVYADFTQSSGDLLALKRAVENGSLASTEAG
KADIKLVFDDGTVYGQSGKLLFRSASVDTTTGQVTLRGEFPNPKGDLLPGLYVRVRIEQAVREKAILIPQRAVIRAADGK
AQVYVVEQGDVAQPRDVELSQSSGNEWVVESGLSAGERLVVDGVQKLQPGAKVAPEEWRSGEVASGDAKEPE

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=43.1937172774869, Blast_Score=295, Evalue=5e-81,
Organism=Escherichia coli, GI1786668, Length=379, Percent_Identity=43.7994722955145, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI1789665, Length=364, Percent_Identity=41.7582417582418, Blast_Score=262, Evalue=2e-71,
Organism=Escherichia coli, GI87082036, Length=339, Percent_Identity=28.6135693215339, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI87081951, Length=146, Percent_Identity=32.8767123287671, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87081796, Length=332, Percent_Identity=25.9036144578313, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41539; Mature: 41539

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMLFHTKSLRCALMLALLLFAGLPAYAQEGGAMPPAAVSVLTLDARPVPVVSELPGRIA
CCCEECCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCCCCCHHHHCCCHHH
ATRVSEVRARVSGILQERVFEQGSLVQQGDVLYRIDPALFRVRVASAEASLERAKATQLN
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECHHHEEEEEHHHHHHHHHHHHHHHH
ARQQLERQKSLRDRDVASGVEYDAAAVALAQADADVALAQAALDEAKINLGYTEVRAPIS
HHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHCCCHHHHHHHHHHHHHEECCHHHHHCCHH
GIIGGALVTEGALVTADAGDALAMIQQIDPVYADFTQSSGDLLALKRAVENGSLASTEAG
HHHHHHHHHCCEEEEECCCHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
KADIKLVFDDGTVYGQSGKLLFRSASVDTTTGQVTLRGEFPNPKGDLLPGLYVRVRIEQA
CCEEEEEEECCEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEHHH
VREKAILIPQRAVIRAADGKAQVYVVEQGDVAQPRDVELSQSSGNEWVVESGLSAGERLV
HHHHHEECCCHHEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCEEEECCCCCCCEEE
VDGVQKLQPGAKVAPEEWRSGEVASGDAKEPE
HHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNMLFHTKSLRCALMLALLLFAGLPAYAQEGGAMPPAAVSVLTLDARPVPVVSELPGRIA
CCCEECCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCCCCCHHHHCCCHHH
ATRVSEVRARVSGILQERVFEQGSLVQQGDVLYRIDPALFRVRVASAEASLERAKATQLN
HHHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEECHHHEEEEEHHHHHHHHHHHHHHHH
ARQQLERQKSLRDRDVASGVEYDAAAVALAQADADVALAQAALDEAKINLGYTEVRAPIS
HHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHCCCHHHHHHHHHHHHHEECCHHHHHCCHH
GIIGGALVTEGALVTADAGDALAMIQQIDPVYADFTQSSGDLLALKRAVENGSLASTEAG
HHHHHHHHHCCEEEEECCCHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCC
KADIKLVFDDGTVYGQSGKLLFRSASVDTTTGQVTLRGEFPNPKGDLLPGLYVRVRIEQA
CCEEEEEEECCEEECCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEEHHH
VREKAILIPQRAVIRAADGKAQVYVVEQGDVAQPRDVELSQSSGNEWVVESGLSAGERLV
HHHHHEECCCHHEEEECCCCEEEEEECCCCCCCCCCCEEECCCCCCEEEECCCCCCCEEE
VDGVQKLQPGAKVAPEEWRSGEVASGDAKEPE
HHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]