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The map label for this gene is psuK [H]

Identifier: 209549553

GI number: 209549553

Start: 2005482

End: 2006426

Strand: Direct

Name: psuK [H]

Synonym: Rleg2_1959

Alternate gene names: 209549553

Gene position: 2005482-2006426 (Clockwise)

Preceding gene: 209549549

Following gene: 209549554

Centisome position: 44.19

GC content: 65.93

Gene sequence:

>945_bases
ATGAGCGGAAAGATTCTCGTGCTCGGCGGTGCCCATATCGATAGACGCGGCCGCATCTCCGGCGAGACGGCGCCTGGCGC
CAGCAATCCCGGCACCTGGTTCGAGGAGCCGGGCGGCGGCGGCTTCAATGCGGCGCGTAATCTGGCAAGGCTTGGTTTTC
AGGTGACGATGATCTCGCCGCGGGGCGGCGATCCGATGGGCGAGACGGTCGGCGCAGCCGCCGATTTCGCCGGCATCGAC
GACCGGCCTTTCGTCTTCCTCGACCGCAAGACCCCGAGCTATACGGCGATTATCGAGAAGGACGGCAATCTGGTCATCGC
CCTTGCCGACATGGATCTCTACCGCTTCTTCGTGCCGCGGCGCCTCTCCATCCGCTGGGTACGGGAGGCCTTCGCCGCCC
ACGATCTCATCCTTTTCGACGCCAACCTGCCGGAAGAAACGATTGCGGCGATCGTCGCCAAGGCGCATTCCCTGGCAAAG
CCGGTCGCAGCGATTGCCATTTCGCCAGCCAAGGTCGTCAGGCTGAAACCCTGCATCGGGGATATCGACTACCTGTTCCT
GAATGAGGCCGAGGCCTCAGCCCTTGCCGGGGAACAGCCGGAAGATGCTGCCGGCTGGCTGCCGCTTCTGAACGAGCTCG
GCATCAGGAACGCCGTCGTCACCCGCGGCCGCCGTGAGCTCGTGGCCCTTTGCGAGGGCCGCGCCGTGACGCTGCAGCCG
CCGATCGCCGACACGGTCGCCGATGTCACCGGAGCCGGCGATTCTCTTGCGGCCGGCACCCTCGCCGCCCTGATGGCCGG
CCTGCCGCTCGAAGAGGCGGTTCGCCACGGCACGGCCGCCGCCGCGCTCACCGTGCAGTCGCGGCACGCCGTCAACGAAA
ATCTGACGCCGGATCTCTTGAATGCGGCGCTTGCCCTTGTTCCCAAGGTCAGAATTCTGCAATGA

Upstream 100 bases:

>100_bases
TTATCGATGCAAAGCAACGGCTTAGGCCTGAAATGCCGATACCCGCGCAAGAGAACCCATCGCATCGATTCGCGTTTCGG
GCATCAAGAGAGTGGCTGCA

Downstream 100 bases:

>100_bases
AGCGGCTAAATTGAAGTTCACCATGATGTCGTCCGAAAACCGCTGAGACTTCGGCATCATGCTCTATCGACGACAGGACA
AGATCATGACCAAGCCCATT

Product: PfkB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MSGKILVLGGAHIDRRGRISGETAPGASNPGTWFEEPGGGGFNAARNLARLGFQVTMISPRGGDPMGETVGAAADFAGID
DRPFVFLDRKTPSYTAIIEKDGNLVIALADMDLYRFFVPRRLSIRWVREAFAAHDLILFDANLPEETIAAIVAKAHSLAK
PVAAIAISPAKVVRLKPCIGDIDYLFLNEAEASALAGEQPEDAAGWLPLLNELGIRNAVVTRGRRELVALCEGRAVTLQP
PIADTVADVTGAGDSLAAGTLAALMAGLPLEEAVRHGTAAAALTVQSRHAVNENLTPDLLNAALALVPKVRILQ

Sequences:

>Translated_314_residues
MSGKILVLGGAHIDRRGRISGETAPGASNPGTWFEEPGGGGFNAARNLARLGFQVTMISPRGGDPMGETVGAAADFAGID
DRPFVFLDRKTPSYTAIIEKDGNLVIALADMDLYRFFVPRRLSIRWVREAFAAHDLILFDANLPEETIAAIVAKAHSLAK
PVAAIAISPAKVVRLKPCIGDIDYLFLNEAEASALAGEQPEDAAGWLPLLNELGIRNAVVTRGRRELVALCEGRAVTLQP
PIADTVADVTGAGDSLAAGTLAALMAGLPLEEAVRHGTAAAALTVQSRHAVNENLTPDLLNAALALVPKVRILQ
>Mature_313_residues
SGKILVLGGAHIDRRGRISGETAPGASNPGTWFEEPGGGGFNAARNLARLGFQVTMISPRGGDPMGETVGAAADFAGIDD
RPFVFLDRKTPSYTAIIEKDGNLVIALADMDLYRFFVPRRLSIRWVREAFAAHDLILFDANLPEETIAAIVAKAHSLAKP
VAAIAISPAKVVRLKPCIGDIDYLFLNEAEASALAGEQPEDAAGWLPLLNELGIRNAVVTRGRRELVALCEGRAVTLQPP
IADTVADVTGAGDSLAAGTLAALMAGLPLEEAVRHGTAAAALTVQSRHAVNENLTPDLLNAALALVPKVRILQ

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: COG0524

COG function: function code G; Sugar kinases, ribokinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788491, Length=296, Percent_Identity=26.6891891891892, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788484, Length=310, Percent_Identity=28.3870967741935, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1790193, Length=279, Percent_Identity=27.2401433691756, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081971, Length=288, Percent_Identity=27.0833333333333, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 33061; Mature: 32930

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGKILVLGGAHIDRRGRISGETAPGASNPGTWFEEPGGGGFNAARNLARLGFQVTMISP
CCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECC
RGGDPMGETVGAAADFAGIDDRPFVFLDRKTPSYTAIIEKDGNLVIALADMDLYRFFVPR
CCCCCCCHHHCCHHHCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCHHHHHHCCC
RLSIRWVREAFAAHDLILFDANLPEETIAAIVAKAHSLAKPVAAIAISPAKVVRLKPCIG
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHEEECCHHEEEEECCCC
DIDYLFLNEAEASALAGEQPEDAAGWLPLLNELGIRNAVVTRGRRELVALCEGRAVTLQP
CCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCEEEECC
PIADTVADVTGAGDSLAAGTLAALMAGLPLEEAVRHGTAAAALTVQSRHAVNENLTPDLL
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCHHEEEEEHHHHHCCCCCCHHHH
NAALALVPKVRILQ
HHHHHHHCCCEECC
>Mature Secondary Structure 
SGKILVLGGAHIDRRGRISGETAPGASNPGTWFEEPGGGGFNAARNLARLGFQVTMISP
CCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEEECC
RGGDPMGETVGAAADFAGIDDRPFVFLDRKTPSYTAIIEKDGNLVIALADMDLYRFFVPR
CCCCCCCHHHCCHHHCCCCCCCCEEEEECCCCCEEEEEECCCCEEEEEECCHHHHHHCCC
RLSIRWVREAFAAHDLILFDANLPEETIAAIVAKAHSLAKPVAAIAISPAKVVRLKPCIG
HHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHHHCCHHHEEECCHHEEEEECCCC
DIDYLFLNEAEASALAGEQPEDAAGWLPLLNELGIRNAVVTRGRRELVALCEGRAVTLQP
CCEEEEECCCHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHCCHHHHHHHHCCCEEEECC
PIADTVADVTGAGDSLAAGTLAALMAGLPLEEAVRHGTAAAALTVQSRHAVNENLTPDLL
CCHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCCHHEEEEEHHHHHCCCCCCHHHH
NAALALVPKVRILQ
HHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]