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The map label for this gene is braC [H]

Identifier: 209549511

GI number: 209549511

Start: 1963315

End: 1964421

Strand: Direct

Name: braC [H]

Synonym: Rleg2_1917

Alternate gene names: 209549511

Gene position: 1963315-1964421 (Clockwise)

Preceding gene: 209549508

Following gene: 209549512

Centisome position: 43.26

GC content: 67.21

Gene sequence:

>1107_bases
ATGGCTAGGGGATCTCCGCCTTTCATGATCAACCTGCGTGGCATAGCAGCTTCTCTGGTGCTGCTGGGAGCGGCGGCGCA
AGGCCATGCGGCCGGCGTGACGATCGGCGTCGTCGCCCCTCAGAACGGCGCGCTCGCCTTGCTTGGCGCTCAGATTGCCG
CCGGCGCCGGTTTCGAGATCCAGCAATCCGGAAACACCCTCGTCGCCATCAACGAGACCTGCGAGGACAATAGCGGCGCT
GCGGTTGCGGATGCGCTGGTCGCTGCCAAGGTGCAGGTCGCGGTCGGCTTTCTCTGCAGCGAGACGCTGGAAGGCGCGCT
GCCGAAGCTGAAAGACGCGGGCATTCCCGCCATGACCATCTCGGCGCGCTCCCGCATCCTGATGGAGGATGCGCTGAAGA
ACGGCTGGCCGCTCTTTCGTCTGGCGCCCGCCGACGGCACTGAGGCGGCAAAGATCAACGAAGTGATCCTGAAGGACTGG
GCCGCCGATCCGATCGCCCTGATCGAAGACGGCACCATCCATGGCCGCGAACTGACCGAAGCGGTGCGCAACGCCCTGGA
ACAGAACGGCCTGAAGCCGGTCTTTACCGATACCTACCGGCCGGGACAGGAGCAGCAGATTGCCCTGGTCCGCCGTCTGA
AGCGCGCCGGCGCCACCCGGGTCTTCATCGGCGGCGACCGCAACGATGTCGCCGTCATCGCCCGCGACGCCAAGGCGGAA
AATATTCCGCTCTCCATTCTCGGCGGCGATGCCATGCGCGCCGCCGACCAGCCGCTGCCGCTTGACCCCGGCGTGCGCGC
CGTCGCCCTGCCCGAATATGCCGTACTGCCGGAAGGCACGGCGGCAGCCGAGGCGCTCAGCGCCAAAGGCATCGAGCCGG
AAGGTTATGTCCTGCCGTCGCTCGCCGCAGCGCTGATCGCCGGCCAGGCGGCGGAAGCTGCGGCCGCCGCCGGCAAGCCG
CTGCAGGAGACGCTCCTCGGCACGACATTCCAGACGCCGGTCGGCGCCATCGCCTTCACTGCTGGGCACGAACTTGCCCA
AAACCCCTACCGCCTGCTCGAATGGCGGGGCAATGGCTTTTTTCCACCTCGCGCGCCGACGCAATGA

Upstream 100 bases:

>100_bases
AGCATCGGTTACCTTTTCTACAATAGGACCAGTGAAAAGCACTGCTTCACGCTTGTTTTTCTTTGACGATCGCAGGCTTG
CGGATAAAAGGGCATGGACG

Downstream 100 bases:

>100_bases
GCGGCGGCCGCCCGTCTTGAAATCGGGCGCCGCGCTCAAACGGCGGCCCACAGGGAGTAAAACTTGATGACGCTGCCCAT
TCGCATTGCCCCCTCGATCC

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MARGSPPFMINLRGIAASLVLLGAAAQGHAAGVTIGVVAPQNGALALLGAQIAAGAGFEIQQSGNTLVAINETCEDNSGA
AVADALVAAKVQVAVGFLCSETLEGALPKLKDAGIPAMTISARSRILMEDALKNGWPLFRLAPADGTEAAKINEVILKDW
AADPIALIEDGTIHGRELTEAVRNALEQNGLKPVFTDTYRPGQEQQIALVRRLKRAGATRVFIGGDRNDVAVIARDAKAE
NIPLSILGGDAMRAADQPLPLDPGVRAVALPEYAVLPEGTAAAEALSAKGIEPEGYVLPSLAAALIAGQAAEAAAAAGKP
LQETLLGTTFQTPVGAIAFTAGHELAQNPYRLLEWRGNGFFPPRAPTQ

Sequences:

>Translated_368_residues
MARGSPPFMINLRGIAASLVLLGAAAQGHAAGVTIGVVAPQNGALALLGAQIAAGAGFEIQQSGNTLVAINETCEDNSGA
AVADALVAAKVQVAVGFLCSETLEGALPKLKDAGIPAMTISARSRILMEDALKNGWPLFRLAPADGTEAAKINEVILKDW
AADPIALIEDGTIHGRELTEAVRNALEQNGLKPVFTDTYRPGQEQQIALVRRLKRAGATRVFIGGDRNDVAVIARDAKAE
NIPLSILGGDAMRAADQPLPLDPGVRAVALPEYAVLPEGTAAAEALSAKGIEPEGYVLPSLAAALIAGQAAEAAAAAGKP
LQETLLGTTFQTPVGAIAFTAGHELAQNPYRLLEWRGNGFFPPRAPTQ
>Mature_367_residues
ARGSPPFMINLRGIAASLVLLGAAAQGHAAGVTIGVVAPQNGALALLGAQIAAGAGFEIQQSGNTLVAINETCEDNSGAA
VADALVAAKVQVAVGFLCSETLEGALPKLKDAGIPAMTISARSRILMEDALKNGWPLFRLAPADGTEAAKINEVILKDWA
ADPIALIEDGTIHGRELTEAVRNALEQNGLKPVFTDTYRPGQEQQIALVRRLKRAGATRVFIGGDRNDVAVIARDAKAEN
IPLSILGGDAMRAADQPLPLDPGVRAVALPEYAVLPEGTAAAEALSAKGIEPEGYVLPSLAAALIAGQAAEAAAAAGKPL
QETLLGTTFQTPVGAIAFTAGHELAQNPYRLLEWRGNGFFPPRAPTQ

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=367, Percent_Identity=24.2506811989101, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789867, Length=374, Percent_Identity=22.9946524064171, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 37958; Mature: 37827

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGSPPFMINLRGIAASLVLLGAAAQGHAAGVTIGVVAPQNGALALLGAQIAAGAGFEI
CCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEHHHHCCCCCEE
QQSGNTLVAINETCEDNSGAAVADALVAAKVQVAVGFLCSETLEGALPKLKDAGIPAMTI
ECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEE
SARSRILMEDALKNGWPLFRLAPADGTEAAKINEVILKDWAADPIALIEDGTIHGRELTE
HHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
AVRNALEQNGLKPVFTDTYRPGQEQQIALVRRLKRAGATRVFIGGDRNDVAVIARDAKAE
HHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCC
NIPLSILGGDAMRAADQPLPLDPGVRAVALPEYAVLPEGTAAAEALSAKGIEPEGYVLPS
CCCEEEECCCHHHHCCCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHCCCCCCCEEHHH
LAAALIAGQAAEAAAAAGKPLQETLLGTTFQTPVGAIAFTAGHELAQNPYRLLEWRGNGF
HHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCHHHHCCCEEEEEECCCCC
FPPRAPTQ
CCCCCCCC
>Mature Secondary Structure 
ARGSPPFMINLRGIAASLVLLGAAAQGHAAGVTIGVVAPQNGALALLGAQIAAGAGFEI
CCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEEEEHHHHCCCCCEE
QQSGNTLVAINETCEDNSGAAVADALVAAKVQVAVGFLCSETLEGALPKLKDAGIPAMTI
ECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEE
SARSRILMEDALKNGWPLFRLAPADGTEAAKINEVILKDWAADPIALIEDGTIHGRELTE
HHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHH
AVRNALEQNGLKPVFTDTYRPGQEQQIALVRRLKRAGATRVFIGGDRNDVAVIARDAKAE
HHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEECCCCC
NIPLSILGGDAMRAADQPLPLDPGVRAVALPEYAVLPEGTAAAEALSAKGIEPEGYVLPS
CCCEEEECCCHHHHCCCCCCCCCCCEEEECCCEEECCCCHHHHHHHHHCCCCCCCEEHHH
LAAALIAGQAAEAAAAAGKPLQETLLGTTFQTPVGAIAFTAGHELAQNPYRLLEWRGNGF
HHHHHHHCCHHHHHHHCCCCHHHHHHCCCCCCCCCEEEEECCHHHHCCCEEEEEECCCCC
FPPRAPTQ
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]